Sequencing Services

2021

Blue laser-induced selective vasorelaxation by the activation of NOSs

SW Park, S Park, HK Choi, HJ Park, W Yu, HS Kim… – Microvascular …, 2021 – Elsevier

… from genome assembly sequence or the representative transcript sequences for aligning to … (Ocimum Biosolutions, India). Gene classification was based on searches done by DAVID (


2020

[HTML] Skin hydration effects of scale-up fermented Cyclopia intermedia against ultraviolet B-induced damage in keratinocyte cells and hairless mice

A Im, SH Yeon, KY Ji, RH Son, KA Um… – … and Alternative Medicine, 2020 – hindawi.com

… In brief, QuantSeq 3′ mRNA-Seq reads were aligned using … assembly sequence or the

representative transcript sequences … (Ocimum Biosolutions, India). Genes were classified based …

[HTML] An overview of pulmonary infections due to rapidly growing mycobacteria in South Asia and impressions from a subtropical region

K Shrivastava, C Kumar, A Singh, A Narang… – International Journal of …, 2020 – ijmyco.org

… by Sanger sequencing of heat-shock protein-65 (hsp65) gene in an Applied Biosystems

Automated Sequencer (Ocimum Biosolutions, Bengaluru, India). Sequences were identified by …


2017

[PDF] Sequence analysis and PCR-RFLP of growth hormone (GH) gene in Vembur and Kilakarsal breeds of sheep

M Seevagan, V Jeichitra, R Rajendran… – Indian Journal of …, 2017 – researchgate.net

… The PCR products were sequenced at M/s. Ocimum Biosolutions, Hyderabad using ABI

3730XL DNA analyzer (Applied Biosystems, USA). The variation in sequences among Vembur …

[HTML] Purinergic receptors P2Y12R and P2X7R: potential targets for PET imaging of microglia phenotypes in multiple sclerosis

W BeainoB Janssen, G Kooij, S van der Pol… – Journal of …, 2017 – Springer

… All oligonucleotides were synthesized by Ocimum Biosolutions (Ocimum Biosolutions,

Ijsselstein, The Netherlands). RNA … All primer sequences are listed in Additional file 1: Table S2.


2016

A study on metabolic prowess of Pseudomonas sp. RPT 52 to degrade imidacloprid, endosulfan and coragen

M Gupta, S MathurTK Sharma, M Rana… – Journal of hazardous …, 2016 – Elsevier

… RPT 52 was identified on the basis of the 16SrDNA sequencing using … to Ocimum biosolutions, India for sequencing. The 16S rDNA sequence of RPT 52 obtained after sequencing was …


2015

[PDF] Cloning and expression of variant chiA from native Serratia marcescens AUDS227

SR Babar, SA More, PU Krishnaraj – Journal of Pure and Applied …, 2015 – researchgate.net

… The corresponding pTZ57R/T plus chiA clones were sequenced using M13 F- 5’… primers

at Ocimum Biosolutions, Hyderabad. After sequencing, the complete nucleotide sequences of …


2013

[HTML] Differentially Expressed Genes during Contrasting Growth Stages of Artemisia annua for Artemisinin Content

P Nair, A Misra, A Singh, AK Shukla, MM Gupta… – PLoS …, 2013 – journals.plos.org… sequences. The program output files were analyzed using TblastParse (an internal report analysis program of Ocimum Biosolutions, … was compared to in house sequences using NCBI’s …

[PDF] High resolution melt curve analysis for the detection of A1, A2 β-casein variants in Indian cows

V Sharma, N Sharma, B Jawed… – … of Microbiology and …, 2013 – researchgate.net

… Sequencing of amplicon for HRMA. Eighty PCR amplified product for β-Casein gene were sent to Ocimum Biosolutions Pvt Ltd, Bangalore for sequencing. Sequence results indicated …

HTML] Methods of cell lysis and effect of detergents for the recovery of nitrile metabolizing enzyme from Amycolatopsis sp. IITR215

V Babu, B Choudhury – Journal of Genetic Engineering and Biotechnology, 2013 – Elsevier… sequenced by Ocimum biosolutions, Hyderabad, India. Forward and reverse16S rRNA gene sequences … The 16S rRNA gene sequence of strain Amycolatopsis sp. IITR215 determined …

Detection of Booroola (FecB) mutation in Indian sheep––Nilagiri

A Sudhakar, R Rajendran, PS Rahumathulla – Small ruminant research, 2013 – Elsevier… The BMPR1B gene sequencing was performed at two independent companies, MWG AG (…and Ocimum Biosolutions (Hyderabad, India) to verify and deduce the nucleotide sequences. …

[PDF] Indigofera himachalensis (Fabaceae: Indigofereae), a new species from Himachal Pradesh, India

V Chauhan, AK PandeyH Schaefer – Phytotaxa, 2013 – researchgate.net… , purified and sequenced at Ocimum Biosolutions, Hyderabad, India and Macrogen, Korea. PCR products were sequenced in both directions and forward and reverse sequences were …

[PDF] Detection of Denitrifying population from upflow anaerobic packed bed column using PCR

V Manoj, N Vasudevan… – Int J Curr Microbiol Appl Sci, 2013 – researchgate.net

… Biosystems) utilizing the commercial sequencing service (Ocimum Biosolutions, … The sequences were identified on the basis of sequence similarity, by comparison with the sequences …


2012 

Molecular characterisation of high-strength polycyclic aromatic hydrocarbon (PAH)-degrading and phenol-tolerant bacteria obtained from thermal power plant …

S Dalal, DP Panigrahi, GS Randhawa, RC Dubey – 2012 – Taylor & Francis … out. The amplified products were analysed using 2% w/v agarose gel. The desired band was cut and eluted. The products were sequenced through the sequencing service provided by Ocimum Biosolutions, Hyderabad, India. …

Enzymatic degradation of textile dye Reactive Orange 13 by newly isolated bacterial strain< i> Alcaligenes faecalis</i> PMS-1

PD Shah, SR Dave, MS Rao – International Biodeterioration & …, 2012 – Elsevier … photolytic activity etc. and based on these results the isolate was identified as Alcaligenes species. 16S rDNA sequencing of isolated bacteria was carried out at Ocimum Biosolutions, Hyderabad, India. The nucleotide sequence …

Cited by 1

[PDF] from jneuroinflammation.com

[PDF] Sphingosine 1-phosphate receptor 5 mediates the immune quiescence of the human brain endothelial barrier

R van Doorn, MAL Pinheiro, G Kooij… – Journal of …, 2012 – jneuroinflammation.com

… All oligonucleotides were synthesized by Ocimum Biosolutions (Ocimum Biosolutions, Ijsselstein,

The Netherlands) (Table 2), RNA was isolated using the … qPCR reactions were performed in an

ABI7900HT sequence detection system with the SYBR Green method (Applied …

View as HTML

[HTML] from isrn.com

[HTML] Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium

R Jha, A Kumar, A Saxena, S Tamuly… – ISRN Veterinary Science, 2012 – isrn.com … The recombinant clones were screened to obtain insert of desired size verified by colony PCR amplification. The cloned product was sequenced by Ocimum Biosolutions

Ltd., Hyderabad. The sequence was submitted to NCBI. 2.3. …

Related articles – Cached – All 3 versions

[CITATION] Quantitative competitive PCR for the detection and quantification of genetically modified cotton event MON-531

D Narra, V Srivatsava – 2012

Simultaneous adsorption and bioaccumulation: A study on continuous mass transfer in column reactor

V Mishra, C Balomajumder… – … Progress & Sustainable …, 2012 – Wiley Online Library

… The same was sent to Ocimum biosolutions, Hyderabad, India for sequencing. The nucleotide sequence similarity searches were conducted using BLAST program of NCBI and the phylogenetic

analysis was carried out using the MEGA package version 4. Carrier Preparation. …

[PDF] from academicjournals.org

[PDF] In vitro characterization of Saccharomyces cerevisiae HM535662 obtained from an indigenous fermented food “Bhaturu” of Western Himalayas

A Sourabh, SS Kanwar… – African Journal of …, 2012 – academicjournals.org

… 3730xl automated sequencer) by using same upstream and downstream primers

(Life Technologies India, Pvt. Ltd), by a commercial sequencing facility (Ocimum

biosolutions, Pvt. Ltd. Hyderabad, India). The corrected sequence …

View as HTML

Optimization of physical parameters for batch mode Zn (II) ion removal from liquid phase: A potential biosorption study

V Mishra, S Dalal, C Balomajumder – Environmental Progress & … – Wiley Online Library

… Amplification was confirmed by running the PCR products on 1% agarose gel. A desired length

was cut from the gel and purified using Bangalore Genei PCR purification kit, and after elution

the product was sent to Ocimum biosolutions for sequencing. …

Related articles

[PDF] from jcbsc.org

[PDF] Journal of Chemical, Biological and Physical Sciences

JA Johnson, T Citarasu, PAM Helen – jcbsc.org

… Fr. Prem Kumar, Correspondent and Secretary, Malankara Catholic College, Mariagiri for his

constant encouragement and support. We are also grateful to CEPC Laboratory, Kollam for the

GC-MS analysis and Ocimum Biosolutions, Hyderabad for Sequencing work. …

View as HTML

[PDF] Differential Gene Expression Profile in Essential Hypertension

P Yang – cdn.intechopen.com

… (47) SHR and BNR for the control Kidney Affymetrix U34A-C microarrays, validation by RT-PCR, DNA sequencing and RFLP analyses. … Affymetrix Scanner 428, Imagene and Genowiz softwares by Ocimum Biosolutions (India) and subjected to arcsine transformation …

View as HTML

Cold-active extracellular α-amylase production from novel bacteria< i> Microbacterium foliorum</i> GA2 and< i> Bacillus cereus</i> GA6 isolated from Gangotri …

M Kuddus, IZ Ahmad – Journal of Genetic Engineering and Biotechnology, 2012 – Elsevier

… processed for sequencing. Sequencing was carried out using the same set of primers

in both the directions to check the validity of the sequence. Sequencing was done by

Ocimum Biosolutions, India. 2.5.3. Antibiotic susceptibility test. …

[PDF] from tubitak.gov.tr

[PDF] Full length cDNA synthesis of differentially displayed ESTs during lactation in the Indian buffalo (Bubalus bubalis)

AK TRIPATHI, KM SINGH, MN VAZE… – Turk. J. Vet. Anim …, 2012 – journals.tubitak.gov.tr

… Sequence analysis Analysis of the 5′ RACE amplified products of the respective ESTs was carried out using Megablast … Seven sense primers were designed and commercially synthesized (OCIMUM BIOSOLUTIONS, Hyderabad) from the 5′ RACE sequences (Table 2) using …

Related articles – View as HTML

[PDF] from ias.ac.in

[PDF] Transcription of gD and gI gene in BHV-1 infected cells

S Chowdhury, B Sharma – ias.ac.in

… RLM product was cloned into Pgemt easy TA vector and was sequenced through vendors (Ocimum Biosolutions, Hyderabad). The sequence was aligned with BHV-1 sequence and the TSS identified from the aligned sequence. Results: Computational analysis: …

Fingolimod attenuates ceramide-induced blood–brain barrier dysfunction in multiple sclerosis by targeting reactive astrocytes

R van Doorn, PG Nijland, N Dekker, ME Witte… – Acta …, 2012 – Springer

… Oligonucleotides were synthesized by Ocimum Biosolu- tions (Ocimum Biosolutions, IJsselstein, The Netherlands). … Quantitative PCR (qPCR) reactions were performed in an ABI7900HT sequence detection system using the SYBR Green method (Applied Biosys- tems, New York …

Cited by 1

Differential expression of silent polyketide biosynthesis gene clusters in chemostat cultures of< i> Aspergillus nidulans</i>

A Sarkar, AN Funk, K Scherlach, F Horn… – Journal of …, 2012 – Elsevier

… Mining the A. nidulans full genome sequence revealed the existence of nearly 50 gene loci encoding polyketide synthases (PKSs) or nonribosomal peptide synthetases … Custom made A. nidulans full genome microarrays (Ocimum Biosolutions) were used in these studies. …

Related articles – All 2 versions

Superparamagnetic Nanoparticles Based Immunomagnetic SeparationMultiplex Polymerase Chain Reaction Assay for Detection of Salmonella

B Padmavathy, A Patel, RV Kumar… – Science of Advanced …, 2012 – ingentaconnect.com

… Four pairs of oligonucleotide primers based on the sequence of invA, prt, fliC, viaB genes are used for detec- tion of the presence of Salmonella Typhi and Salmonella … All the oligonucleotide primers were custom synthesized from Ocimum Biosolutions Pvt Ltd., Netherlands. …

Identification and distribution of putative virulence genes in clinical strains of Yersinia enterocolitica biovar 1A by suppression subtractive hybridization

P Kumar, JS Virdi – Journal of Applied Microbiology, 2012 – Wiley Online Library

… sequenced directly using appropriate primers in either one or both directions. The commercial

facilities provided by M/s Link Biotech (Ocimum Biosolutions, Delhi) and M/S Lab India (Gurgaon,

India) were used for sequencing of the samples. The sequences obtained were …

[PDF] from virologyj.com

[PDF] Non structural protein of avian influenza A (H11N1) virus is a weaker suppressor of immune responses but capable of inducing apoptosis in host cells

S Mukherjee, S Majumdar, VC Vipat, AC Mishra… – Virology …, 2012 – virologyj.com

… Amplicons cloned into pcDNA 3.0(-) expression vector (Invitrogen, Carlsbad, CA) were

screened by sequencing. … Data was analyzed using GENOWIZ Microarray and Pathway

analysis tool (Ocimum Biosolutions, Hyderabad, India). …

View as HTML

Polymorphism in RD1 locus and its effect on down stream genes among South Indian clinical isolates of M. tuberculosis

AK Refaya, SS Kumar, S Balaji… – Journal of Medical …, 2012 – Soc General Microbiol

… earlier (Baess 1974). Spoligotyping was performed with a commercially available

kit 111 (Ocimum biosolutions, Hyderabad, India) according to the instructions of the

112 manufacturer. … 134 135 Automated DNA sequencing 136 …

A novel mechanism of gall midge resistance in the rice variety Kavya revealed by microarray analysis

N Rawat, N Chiruvuri Naga… – Functional & integrative …, 2012 – Springer… RNA, target hybridization, as well as washing, staining, and scanning of the arrays were carried out by Ocimum Biosolutions (Hyderabad, India … The data were analyzed using the 7500 Sequence Detection Software (Applied Biosystems, USA) with default baseline and threshold. …

Cited by 2

[PDF] from ias.ac.in

[PDF] Inhibition of HIV-1 Integrase gene expression by 10-23 DNAzyme

N Singh, A Ranjan, S Sur, R Chandra, V Tandon – Journal of biosciences, 2012 – ias.ac.in

… The sequence GGCTAGCTACAACGA was a specific catalytic domain of 10-23 DNAzyme molecule, which was flanked by two substrate recognition arm of seven to nine deoxynucleo- tides. All the DNAzymes were synthesized by Ocimum Biosolutions, Hyderabad, India. …

Cited by 1 – View as HTML

GenoType MTBDRsl performance on clinical samples with diverse genetic background

P Miotto, AM Cabibbe, P Mantegani… – European …, 2012 – Eur Respiratory Soc

… Sequencing. Genomic regions harbouring drug-resistance mutations were amplified and … The amplified direct repeat locus of clinical isolates was investigated using the Spoligotyping kit (Ocimum Biosolutions, Hyderabad, India) following the manufacturer’s instructions. …

Cited by 1 – Related articles – All 2 versions

[PDF] from niscair.res.in

A promising strain of Streptomyces sp. with agricultural traits for growth promotion and disease management

M Alam, S Dharni, SKS Abdul-Khaliq… – Indian Journal of …, 2012 – nopr.niscair.res.in … Primers were procured from Ocimum Biosolutions, India. … The recombinants were selected by

blue/white screening and recombinant plasmid DNA was isolated for further restriction analysis and sequencing of the insert on a 3130XL Genetic analyzer (Applied Biosystems, USA …

Rapid and Sensitive Detection of Major Uropathogens in a Single-Pot Multiplex PCR Assay

B Padmavathy, R Vinoth Kumar, A Patel… – Current …, 2012 – Springer

… Comparison with closely related species was found to predict no homology or less homology with the primer sequence used in the mPCR assay. … All the primers were synthesized or custom-order from Ocimum Biosolutions, Netherlands. Optimization of Uniplex and mPCR …

Performance comparison of multiple microarray platforms for gene expression profiling.

F Liu, WP Kuo, TK Jenssen, E Hovig – Methods in molecular biology ( …, 2012 – Springer

… Following recent studies (17–19), we used probe sequence information to map probes both on levels of genes and … Biosystems (ABI), Amersham (now GE Healthcare), Compugen (now Sigma-Genosys), Mergen, MWG BioTech (now Ocimum Biosolutions), Operon, “academic …

[PDF] from tjpr.org

Detection of Salmonella enterica serovars in shrimps in eight hours by multiplex PCR assay

G Jeyasekaran, KT Raj, RJ Shakila, AJ Thangarani… – Annals of …, 2012 – Springer

… 1992), while fimA, coding for the fimbrial gene, demonstrates a high degree of sequence conservation among Salmonella serovars (Cohen et al. 1996; Townsend et al. 2001). … All primer sequences were synthe- sized by Ocimum Biosolutions (Indianapolis, IN). …

Related articles – All 2 versions

Antagonism of microRNA Function in Zebrafish Embryos by Using Locked Nucleic Acid Enzymes (LNAzymes)

H Suryawanshi, MK Lalwani, S Ramasamy… – …, 2012 – Wiley Online Library

… was exactly as previously described.18 All DNA and LNA oligonucleotides used in this study

were purchased from Ocimum Biosolutions (Hyderabad, India … RT products were then used for

real-time PCR with mature miRNA sequence as the forward primer and the universal …

Cited by 1 – Related articles – All 3 versions

Demonstration of sequential adaptation strategy for developing salt tolerance in bacteria for wastewater treatment: A study using< i> Escherichia coli</i> as model

S Puranik, S Shaligram, V Paliwal, DV Raje… – Bioresource …, 2012 – Elsevier

… The expression data for each sample was obtained on OciChip E.coli K-12 gene chip (M/s Ocimum Biosolutions, Hyderabad); the gene probe details with chip have been described in Supplementary Table 1. The … Term and GO ID, Primer Sequence(5′-3′), Product length (bp). …

Gene expression profile of early in vitro biofilms of Streptococcus pneumoniae

MK Yadav, SK Kwon, CG Cho, SW Park… – Microbiology and …, 2012 – Wiley Online Library

… Microarray data analysis was carried out by Genowiz 4.0TM (Ocimum Biosolutions, Hyderabad, India). Global lowess was used for data analysis with normalization. … Primers were designed by

standard procedures from nucleotide sequence of S. pneumoniae R6 strain …

New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Gene Expression Profiling of Escherichia coli in Response to Interactions with the Lettuce Rhizosphere

Z Hou, RC Fink, E Black, M Sugawara… – Journal of Applied …, 2012 – Wiley Online Library

… 2007). 2.4 Microarray expression analysis. Triplicate E. coli O157 microarrays (6 slides)

(OciChip™,Ocimum Biosolutions Inc., Indianapolis, IN) were used in this study. … primers listed

in Table 2, using an ABI 7500 real-time PCR system with sequence detection …

[PDF] from mdpi.com

The Effect of Secretory Factors of Adipose-Derived Stem Cells on Human Keratinocytes

KM Moon, YH Park, JS Lee, YB Chae, MM Kim… – International journal of …, 2012 – mdpi.com … Trypsin spot Protein name Accession # Protein score Pep. count Position Sequence coverage 303 Type VI collagen, alpha-2, isoform 2C2a’ NP_478055 86 9 96–393 12% … Trypsin spot Protein name Accession # Protein score Pep. count Position Sequence coverage …

Cited by 2 – Related articles – All 5 versions

[PDF] Yersiniosis and Food Safety

K Isshiki, RC Ray, D Montet – downloads.hindawi.com

… 1, no. 4, pp. 323– 333, 1999. [7] X. Wang, Y. Li, H. Jing et al., “Complete genome sequence of a Yersinia enterocolitica “old world” (3/O:9) strain and comparison with the “new world” (1B/O:8) strain,” Journal of Clinical Microbiology, vol. 49, no. 4, pp. … 2.1.1. Sequence Retrieval. …

View as HTML

[HTML] Functional Genomic Analysis of Candida glabrata-Macrophage Interaction: Role of Chromatin Remodeling in Virulence

MN Rai, S Balusu, N Gorityala, L Dandu, R Kaur – PLoS Pathogens, 2012 – dx.plos.org … the screen. Tn7 insertion in the identified mutants was mapped, by transposon rescue and sequencing analyses, to 56 C. glabrata ORFs and 20 intergenic regions

(http://www.genolevures.org) (Tables S1 and S2). Using the …


2012 

Molecular characterisation of high-strength polycyclic aromatic hydrocarbon (PAH)-degrading and phenol-tolerant bacteria obtained from thermal power plant …

S Dalal, DP Panigrahi, GS Randhawa, RC Dubey - 2012 - Taylor & Francis

The amplified products were analysed using 2% w/v agarose gel. The desired band was cut and eluted. The products were sequenced through the sequencing service provided by Ocimum Biosolutions, Hyderabad, India.

Cited by 1 - Related articles

Enzymatic degradation of textile dye Reactive Orange 13 by newly isolated bacterial strain< i> Alcaligenes faecalis</i> PMS-1

PD Shah, SR Dave, MS Rao - International Biodeterioration & …, 2012 - Elsevier

... photolytic activity etc. and based on these results the isolate was identified as Alcaligenes species. 16S rDNA sequencing of isolated bacteria was carried out at Ocimum Biosolutions, Hyderabad, India. The nucleotide sequence ...Cited by 1 [PDF] from jneuroinflammation.com

[PDF] Sphingosine 1-phosphate receptor 5 mediates the immune quiescence of the human brain endothelial barrier

R van Doorn, MAL Pinheiro, G Kooij… - Journal of …, 2012 - jneuroinflammation.com

All oligonucleotides were synthesized by Ocimum Biosolutions (Ocimum Biosolutions, Ijsselstein, The Netherlands) (Table 2), RNA was isolated using the ... qPCR reactions were performed in an ABI7900HT sequence detection system with the SYBR Green method (Applied ...View as HTML [HTML] from isrn.com

[HTML] Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium

R Jha, A Kumar, A Saxena, S Tamuly… - ISRN Veterinary Science, 2012 - isrn.com

The recombinant clones were screened to obtain insert of desired size verified by  colony PCR amplification. The cloned product was sequenced by Ocimum Biosolutions Ltd., Hyderabad. The sequence was submitted to NCBI. 

Related articles - Cached - All 3 versions

[CITATION] Quantitative competitive PCR for the detection and quantification of genetically modified cotton event MON-531

D Narra, V Srivatsava - 2012

Simultaneous adsorption and bioaccumulation: A study on continuous mass transfer in column reactor

V Mishra, C Balomajumder… - … Progress & Sustainable …, 2012 - Wiley Online Library

The same was sent to Ocimum biosolutions, Hyderabad, India for sequencing. The nucleotide sequence similarity searches were conducted using BLAST program of NCBI and the phylogenetic analysis was carried out using the MEGA package version 4. Carrier Preparation.[PDF] from academicjournals.org

[PDF] In vitro characterization of Saccharomyces cerevisiae HM535662 obtained from an indigenous fermented food “Bhaturu” of Western Himalayas

A Sourabh, SS Kanwar… - African Journal of …, 2012 - academicjournals.org

... 3730xl automated sequencer) by using same upstream and downstream primers (Life Technologies India,Pvt. Ltd), by a commercial sequencing facility (Ocimum biosolutions, Pvt. Ltd. Hyderabad, India). View as HTML

Optimization of physical parameters for batch mode Zn (II) ion removal from liquid phase: A potential biosorption study

V Mishra, S Dalal, C Balo Majumder - Environmental Progress & … - Wiley Online Library

Amplification was confirmed by running the PCR products on 1% agarose gel. A desired length was cut from the gel and purified using Bangalore Genei PCR purification kit, and after elution the product was sent to Ocimum biosolutions for sequencing. Related articles [PDF] from jcbsc.org

[PDF] Journal of Chemical, Biological and Physical Sciences

JA Johnson, T Citarasu, PAM Helen - jcbsc.org

Fr. Prem Kumar, Correspondent and Secretary, Malankara Catholic College, Mariagiri for his constant encouragement and support. We are also grateful to CEPC Laboratory, Kollam for the GC-MS analysis and Ocimum Biosolutions, Hyderabad for Sequencing work. View as HTML

[PDF] Differential Gene Expression Profile in Essential Hypertension

P Yang - cdn.intechopen.com

(47) SHR and BNR for the control Kidney Affymetrix U34A-C microarrays, validation by RT-PCR, DNA sequencing and RFLP analyses. ... Affymetrix Scanner 428, Imagene and Genowiz softwares by Ocimum Biosolutions (India) and subjected to arsinh transformation View as HTML

Cold-active extracellular α-amylase production from novel bacteria< i> Microbacterium foliorum</i> GA2 and< i> Bacillus cereus</i> GA6 isolated from Gangotri …

M Kuddus, IZ Ahmad - Journal of Genetic Engineering and Biotechnology, 2012 - Elsevier

... processed for sequencing. Sequencing was carried out using the same set of primers in both directions to check the validity of the sequence. Sequencing was done by Ocimum Biosolutions, India. [PDF] from tubitak.gov.tr

[PDF] Full length cDNA synthesis of differentially displayed ESTs during lactation in the Indian buffalo (Bubalus bubalis)

AK TRIPATHI, KM SINGH, MN VAZE… - Turk. J. Vet. Anim …, 2012 - journals.tubitak.gov.tr

 Sequence analysis Analysis of the 5' RACE amplified products of the respective ESTs was carried  out using Megablast. Seven sense primers were designed and commercially synthesized (OCIMUM BIOSOLUTIONS, Hyderabad) Related articles - View as HTML [PDF] from ias.ac.in

[PDF] Transcription of gD and gI gene in BHV-1 infected cells

S Chowdhury, B Sharma - ias.ac.in

RLM product was cloned into Pgemt easy TA vector and was sequenced through vendors (Ocimum Biosolutions, Hyderabad). The sequence was aligned with BHV-1 sequence and theTSS identified from the aligned sequence. Results: Computational analysis: ...View as HTML

Fingolimod attenuates ceramide-induced blood–brain barrier dysfunction in multiple sclerosis by targeting reactive astrocytes

R van Doorn, PG Nijland, N Dekker, ME Witte… - Acta …, 2012 - Springer

Oligonucleotides were synthesized by Ocimum Biosolutions (Ocimum Biosolutions, IJsselstein, The Netherlands). ... Quantitative PCR (qPCR) reactions were performed in an ABI7900HT sequence detection system using the SYBR Green method (Applied Biosys- tems, New York Cited by 1

Differential expression of silent polyketide biosynthesis gene clusters in chemostat cultures of< i> Aspergillus nidulans</i>

A Sarkar, AN Funk, K Scherlach, F Horn… - Journal of …, 2012 - Elsevier

... Mining the A. nidulans full genome sequence revealed the existence of nearly 50 gene loci encoding polyketide synthases (PKSs) or nonribosomal peptide synthetases ... Custom made A.nidulans full genome microarrays (Ocimum Biosolutions) were used in these studies. .Related articles - All 2 versions

Superparamagnetic Nanoparticles Based Immunomagnetic SeparationMultiplex Polymerase Chain Reaction Assay for Detection of Salmonella

B Padmavathy, A Patel, RV Kumar… - Science of Advanced …, 2012 - ingentaconnect.com

... Four pairs of oligonucleotide primers based on the sequence of invA, prt, fliC, viaB genes are used for detec- tion of the presence of Salmonella Typhi and Salmonella ... All the oligonucleotide primers were custom synthesized from Ocimum Biosolutions Pvt Ltd., Netherlands. ...

Identification and distribution of putative virulence genes in clinical strains of Yersinia enterocolitica biovar 1A by suppression subtractive hybridization

P Kumar, JS Virdi - Journal of Applied Microbiology, 2012 - Wiley Online Library

... sequenced directly using appropriate primers in either one or both directions. The commercial facilities provided by M/s Link Biotech (Ocimum Biosolutions, Delhi) and M/S Lab India (Gurgaon, India) were used for sequencing of the samples. The sequences obtained were ...[PDF] from virologyj.com

[PDF] Non structural protein of avian influenza A (H11N1) virus is a weaker suppressor of immune responses but capable of inducing apoptosis in host cells

S Mukherjee, S Majumdar, VC Vipat, AC Mishra… - Virology …, 2012 - virologyj.com

... Amplicons cloned into pcDNA 3.0(-) expression vector (Invitrogen, Carlsbad, CA) were screened by sequencing. ... Data was analyzed using GENOWIZ Microarray and Pathway analysis tool (Ocimum Biosolutions, Hyderabad, India). ...View as HTML

 

Polymorphism in RD1 locus and its effect on down stream genes among South Indian clinical isolates of M. tuberculosis

AK Refaya, SS Kumar, S Balaji… - Journal of Medical …, 2012 - Soc General Microbiol

... earlier (Baess 1974). Spoligotyping was performed with a commercially available kit 111 (Ocimum biosolutions, Hyderabad, India) according to the instructions of the 112 manufacturer. ... 134 135 Automated DNA sequencing 136 ...

A novel mechanism of gall midge resistance in the rice variety Kavya revealed by microarray analysis

N Rawat, N Chiruvuri Naga… - Functional & integrative …, 2012 - Springer

... RNA, target hybridization, as well as washing, staining, and scanning of the arrays were carried out by Ocimum Biosolutions (Hyderabad, India ... The data were analyzed using the 7500 Sequence Detection Software (Applied Biosystems, USA) with default baseline and threshold. ...Cited by 2 [PDF] from ias.ac.in

 

[PDF] Inhibition of HIV-1 Integrase gene expression by 10-23 DNAzyme

N Singh, A Ranjan, S Sur, R Chandra, V Tandon - Journal of biosciences, 2012 - ias.ac.in

... The sequence GGCTAGCTACAACGA was a specific catalytic domain of 10-23 DNAzyme molecule, which was flanked by two substrate recognition arm of seven to nine deoxynucleotides. All the DNAzymes were synthesized by Ocimum Biosolutions, Hyderabad, India. ...Cited by 1 - View as HTML

GenoType MTBDRsl performance on clinical samples with diverse genetic background

P Miotto, AM Cabibbe, P Mantegani… - European …, 2012 - Eur Respiratory Soc

... Sequencing. Genomic regions harbouring drug-resistance mutations were amplified and ... The amplified direct repeat locus of clinical isolates was investigated using the Spoligotyping kit (Ocimum Biosolutions, Hyderabad, India) following the manufacturer's instructions. ...Cited by 1 - Related articles - All 2 versions

 

A promising strain of Streptomyces sp. with agricultural traits for growth promotion and disease management

M Alam, S Dharni, SKS Abdul-Khaliq… - Indian Journal of …, 2012 - nopr.niscair.res.in

... Primers were procured from Ocimum Biosolutions, India. ... The recombinants were selected byblue/white screening and recombinant plasmid DNA was isolated for further restriction analysis and sequencing of the insert on a 3130XL Genetic analyzer (Applied Biosystems, USA ...

Rapid and Sensitive Detection of Major Uropathogens in a Single-Pot Multiplex PCR Assay

B Padmavathy, R Vinoth Kumar, A Patel… - Current …, 2012 - Springer

... Comparison with closely related species was found to predict no homology or less homology with the primer sequence used in the mPCR assay. ... All the primers were synthesized on custom-order from Ocimum Biosolutions, Netherlands. Optimization of Uniplex and mPCR ...

Performance comparison of multiple microarray platforms for gene expression profiling.

F Liu, WP Kuo, TK Jenssen, E Hovig - Methods in molecular biology ( …, 2012 - Springer

... Following recent studies (17–19), we used probe sequence information to map probes both on levels of genes and ... Biosystems (ABI), Amersham (now GE Healthcare), Compugen (now Sigma-Genosys), Mergen, MWG BioTech (now Ocimum Biosolutions), Operon, “academic … All 2 versions [PDF] from tjpr.org

Detection of Salmonella enterica serovars in shrimps in eight hours by multiplex PCR assay

G Jeyasekaran, KT Raj, RJ Shakila, AJ Thangarani… - Annals of …, 2012 - Springer... 1992), while fimA, coding for the fimbrial gene, demonstrates a high degree of sequence conservation among Salmonella serovars (Cohen et al. 1996; Townsend et al. 2001). ... All primer sequences were synthe- sized by Ocimum Biosolutions (Indianapolis, IN). ...Related articles - All 2 versions

Antagonism of microRNA Function in Zebrafish Embryos by Using Locked Nucleic Acid Enzymes (LNAzymes)

H Suryawanshi, MK Lalwani, S Ramasamy… - …, 2012 - Wiley Online Library

... was exactly as previously described.18 All DNA and LNA oligonucleotides used in this study were purchased from Ocimum Biosolutions (Hyderabad, India ... RT products were then used for real-time PCR with mature miRNA sequence as the forward primer and the universal ...Cited by 1 - Related articles - All 3 versions

 

Demonstration of sequential adaptation strategy for developing salt tolerance in bacteria for wastewater treatment: A study using< i> Escherichia coli</i> as model

S Puranik, S Shaligram, V Paliwal, DV Raje… - Bioresource …, 2012 - Elsevier... The expression data for each sample was obtained on OciChip E.coli K-12 gene chip (M/s Ocimum Biosolutions, Hyderabad); the gene probe details with chip have been described in Supplementary Table 1. The ... Term and GO ID, Primer Sequence(5'-3'), Product length (bp). ...

Gene expression profile of early in vitro biofilms of Streptococcus pneumoniae

MK Yadav, SK Kwon, CG Cho, SW Park… - Microbiology and …, 2012 - Wiley Online Library

... Microarray data analysis was carried out by Genowiz 4.0TM (Ocimum Biosolutions, Hyderabad, India). Global lowess was used for data analysis with normalization. ... Primers were designed by standard procedures from nucleotide sequence of S. pneumoniae R6 strain ...

New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Gene Expression Profiling of Escherichia coli in Response to Interactions with the Lettuce Rhizosphere

Z Hou, RC Fink, E Black, M Sugawara… - Journal of Applied …, 2012 - Wiley Online Library

... 2007). 2.4 Microarray expression analysis. Triplicate E. coli O157 microarrays (6 slides) (OciChip™,Ocimum Biosolutions Inc., Indianapolis, IN) were used in this study. ... primers listed in Table 2, using an ABI 7500 real-time PCR system with sequence detection ...[PDF] from mdpi.com

The Effect of Secretory Factors of Adipose-Derived Stem Cells on Human Keratinocytes

KM Moon, YH Park, JS Lee, YB Chae, MM Kim… - International journal of …, 2012 - mdpi.com

... Trypsin spot Protein name Accession # Protein score Pep. count Position Sequence coverage 303 Type VI collagen, alpha-2, isoform 2C2a' NP_478055 86 9 96–393 12% ... Trypsin spot Protein name Accession # Protein score Pep. count Position Sequence coverage ...Cited by 2 - Related articles - All 5 versions

[PDF] Yersiniosis and Food Safety

K Isshiki, RC Ray, D Montet - downloads.hindawi.com

... 1, no. 4, pp. 323– 333, 1999. [7] X. Wang, Y. Li, H. Jing et al., “Complete genome sequence of a Yersinia enterocolitica “old world” (3/O:9) strain and comparison with the “new world” (1B/O:8) strain,” Journal of Clinical Microbiology, vol. 49, no. 4, pp. ... 2.1.1. Sequence Retrieval. ...View as HTML

 [HTML] Functional Genomic Analysis of Candida glabrata-Macrophage Interaction: Role of Chromatin Remodeling in Virulence

MN Rai, S Balusu, N Gorityala, L Dandu, R Kaur - PLoS Pathogens, 2012 - dx.plos.org

... the screen. Tn7 insertion in the identified mutants was mapped, by transposon rescue and sequencing analyses, to 56 C. glabrata ORFs and 20 intergenic regions (http://www.genolevures.org) (Tables S1 and S2). Using the ...

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2011

Molecular cloning, overexpression, purification, crystallization and preliminary X-ray diffraction studies of histidinol phosphate aminotransferase (HisC2) from …

N Nasir, R Vyas, C Chugh… - … Section F: Structural …, 2011 - scripts.iucr.org

... positive clones using the same restriction enzymes. The sequence and the directionality of the clone were confirmed by DNA sequencing (Ocimum Biosolutions, India). A construct with the following expression sequence, consisting ...Related articles - All 3 versions

 

[PDF] Potential of Alkaline Protease Isolated from Thermoactinomyces sp. RM4 as an Alternative to Conventional Chemicals in Leather Industry Dehairing Process

[PDF] from ndpublisher.in

A Verma, HS Pal, R Singh… - International Journal of …, 2011 - ndpublisher.in

... Bergey's manual (Pillai and Archana, 2008). Approximately 1,500- bp-sequence information of amplified fragment was obtained through the sequencing services provided by Ocimum Biosolutions Pvt. Ltd., Hyderabad, India. ...Cited by 1 - Related articles - View as HTML - All 5 versions

 

Roohi, M. Kuddus, IZ Ahmad and JM Arif

[HTML] from scialert.net

ISI Thomson - Asian Journal of Biotechnology, 2011 - scialert.net

... The PCR amplicon for the partial 16S rRNA gene was further processed for sequencing. Sequencing was carried out using the same set of primers in both the directions to check the validity of the sequence. Sequencing was done by Ocimum Biosolutions, India. ...Related articles - Cached

 

 

[PDF] Production of Cold-active Extracellular a-Amylase by Newly Isolated Microbacterium foliorum GA2 from Gangotri glacier, Western Himalaya, India

[PDF] from docsdrive.com

MK Roohi, IZ Ahmad… - Asian Journal of Biotechnology, 2011 - docsdrive.com ... Sequencing was done by Ocimum Biosolutions, lndia. Antibiotic susceptibility and plasmid curing: The antibiotic susceptibility of the isolate was studied on Muller-Hinton agar media by disc diffusion methods (Bauer elf al., 1966). ...Cited by 2 - Related articles - All 2 versions

 

HisB from Mycobacterium tuberculosis: cloning, overexpression in Mycobacterium smegmatis, purification, crystallization and preliminary X-ray crystallographic …

MS Ahangar, Y Khandokar, N Nasir… - … Section F: Structural …, 2011 - scripts.iucr.org... using T4 DNA ligase. Successful integration of the insert into the expression vector and its directionality were confirmed by DNA sequencing (Ocimum Biosolutions,Hyderabad, India). Table 1 Primers used for PCR amplification. ...Cited by 1 - Related articles - All 3 versions

 

 

2007

Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6

[HTML] from nih.gov

JA Lanie, WL Ng, KM Kazmierczak… - Journal of …, 2007 - Am Soc Microbiol ... As was done previously (48), we inserted a placeholder sequence containing the five repeats reported previously for R6 into this region of the D39 genome sequence (see the text). ... (now available from Ocimum Biosolutions). ...Cited by 106 - Related articles - BL Direct - All 11 versions