Spoligotyping

Mapmygenome acquired the rights to sell Spoligotyping from Ocimum Biosolutions in 2014. 

Ocimum acquired the spoligotyping kit through an acquisition of the Biomolecules division of Isogen Lifesciences based in The Netherlands. Since the acquisition in 2006, Ocimum has continued to invest in additional R&D and is in the process of adding several new kits to its portfolio.

As with other products, below is a chronological publication reference to the spoligotyping kit. The earliest publication for this product is dated way back in 1997. Many publications will refer to Isogen as that was the name of our subsidiary. Recently we changed the name back to Ocimum Biosolutions but we continue to find references to Isogen.

With the large number of references of over 400 and from labs across the world, the spoligotyping kit has been validated for over 15 years now giving confidence to researchers across the globe using it.

 

2022

 

Era of TB elimination: Growing need to understand diversities of Mycobacterium tuberculosis lineages!

A Tejashree, PA Mahesh, MK Karthik, A Azeem… - Indian Journal of …, 2022 - Elsevier

… The Spoligotyping was carried out by amplifying the whole DR region using the commercially available kit (Mapmygenome, Hyderabad) according to a standardized method using the …

 

[HTML] Molecular Epidemiology and Polymorphism Analysis in Drug-Resistant Genes in M. tuberculosis Clinical Isolates from Western and Northern India

V Rana, N Singh, C Nikam, P Kambli… - Infection and Drug …, 2022 - ncbi.nlm.nih.gov

… kit (Ocimum Biosolutions, Hyderabad). The standardized spoligotyping protocol has

been described previously. Briefly, the cycling conditions used for spoligotyping were: initial 

 

[HTML] Spoligotyping of Clinical Isolates of Mycobacterium tuberculosis Complex Species in the Oromia Region of Ethiopia

B Hussien, A Zewude, B Wondale, A Hailu… - Frontiers in Public …, 2022 - ncbi.nlm.nih.gov

… (29) and as per the spoligotype kit supplier's instructions (Ocimum Biosolutions, Ijsselstein, The Netherlands). DNA from known strains of M. bovis SB1176 and M. …

 

 

 

2021

 

Bovine tuberculosis in Taiwan, 2008–2019

TH Chan, CS Huang, C Tu, R Jou - … and Emerging Diseases, 2021 - Wiley Online Library

… Spacer oligonucleotide typing (spoligotyping) A commercially available kit (Ocimum Biosolutions, … that was covalently precoated with a set of 43 spacer oligonucleotides derived from the …

 

Whole genome sequencing analysis of multidrug-resistant tuberculosis in Singapore, 2006–2018

CBE Chee, LKY Lim, RTH Ong, LH Sng, LY Hsu… - European Journal of …, 2021 - Springer

… Spoligotyping and MIRU-VNTR methods identified 108 patients in 24 clusters. The Beijing

spoligotype … One cluster belonged to the EAI spoligotype and another to the H spoligotype. …

 

[HTML] Multispacer Sequence Typing for Mycobacterium bovis Genotyping

ÉB Sales, AA Fonseca Jr, CM Gonçalves… - Frontiers in Veterinary …, 2021 - ncbi.nlm.nih.gov

… Among the remaining techniques, spacer oligonucleotide typing (spoligotyping; based on … (41) using spolygotyping kit by Ocimum Biosolutions. The identification of individual 

 

 

2020

 

[HTML] Spoligotyping and drug sensitivity of Mycobacterium tuberculosis isolated from pulmonary tuberculosis patients in the Arsi Zone of southeastern Ethiopia

B Haile, K Tafess, A Zewude, B Yenew, G Siu… - New Microbes and New …, 2020 - Elsevier

… (Ocimum Biosolutions, Ijsselstein, the Netherlands). The direct repeat (DR) region was amplified by PCR using oligonucleotide … of 43 immobilized oligonucleotides, each corresponding …

 

DRUG RESISTANCE OF MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATES FROM NEW TB CASES IN KAZAKHSTAN

U Kozhamkulov, A Akhmetova, S Rakhimova… - 2020 - nur.nu.edu.kz

… drug resistance, genotyping and whole genome sequencing. Methods: 192M.tuberculosis … available kit “Ocimum Biosolutions Inc”. The whole genome sequencing was carried out on …

 

[HTML] Spoligotype Variation of Mycobacterium tuberculosis Strains Prevailing in Korea

SE Song, DH Kim, SH Kim, MS Park… - Canadian Journal of …, 2020 - hindawi.com

… Spoligotyping was performed as described by the manufacturer (Ocimum Biosolutions). E binary spoligotype formats were compared with the SITVIT2 database …

 

2019

[PDF] UPDATE ON VIRULENCE FACTORS IN MYCOBACTERIA

R Brosch - Инфекция и иммунитет, 2019 - iimmun.ru… cially available kit (Mapmygenome India Ltd., Madhapur, India) according to the manufacturer's protocol …Spoligotypes were obtained for 127 (97.8%) isolates … When spoligotyping and MIRU-VNTR typing was used in com- bination, only tw o, out of 130 isolates, clustered both in …[HTML] plos.org

 

[HTML] Genetic diversity of Mycobacterium tuberculosis strains circulating in Botswana

T Mogashoa, P Melamu, SD Ley, EM Streicher… - PloS one, 2019 - journals.plos.org… A commercially available spoligotyping kit (Mapmygenome, Hyderabad India) was used for amplification, hybridization and detection of … RIF or to both INH and RIF and which clustered according to spoligotyping (n = 65 … A total of 458/461 isolates were successfully spoligotyped …

 

Genetic diversity of Mycobacterium tuberculosis isolates causing pulmonary and extrapulmonary tuberculosis in the capital of Iran

S Hadifar, L Shamkhali, MK Kamakoli… - Molecular phylogenetics …, 2019 - Elsevier… All M.tb isolates were analyzed with Spoligotyping commercial kit (Mapmygenome Genomics company … 1. Distribution of M.tb genotypes, SIT and corresponding spoligotype patterns of all … There was 100% concordance between Spoligotyping and MIRU-VNTR data (except for …

 

Association of ubiA mutations and high-level of ethambutol resistance among Mycobacterium tuberculosis Thai clinical isolates

O Tulyaprawat, A Chaiprasert, P Chongtrakool… - Tuberculosis, 2019 - Elsevier… Spoligotyping was performed according to the manufacturer's instructions (Mapmygenome, India) [17] … The octal code format of each strain was entered to assign Spoligotype International Type (SIT) and phylogenetic clade by using SITVITWEB, a database of the Pasteur …[HTML] frontiersin.org

 

Bayesian Assessment of the Accuracy of a PCR-Based Rapid Diagnostic Test for Bovine Tuberculosis in Swine

S Barandiaran, MS Perez Aguirreburualde… - Frontiers in veterinary …, 2019 - frontiersin.org… Spoligotyping was carried out using the spoligotyping kit (Mapmygenome India) … remaining 21% culture and PCR-negative samples (ie, 4.9% of all the samples) were M. avium complex (IS1245+)-positive (Table 3). All IS6110-positive samples showed spoligotypes that were …

 

Prevalence of Mycobacterium bovis infection in traditionally managed cattle at the wildlife-livestock interface in South Africa in the absence of control measures

PR Sichewo, EMC Etter, AL Michel - Veterinary Research …, 2019 - Springer… Spoligotyping assay was performed using a commercially available kit (Mapmygenome India Ltd.) to … Spoligotyping confirmed the species identification of the 12 isolates from cattle and 22 from … the isolates showed identical profiles belonging to the SB 0130 spoligotype, which is …Related articles All 2 versions

 

Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

PAM Carneiro, TN Pasquatti, H Takatani… - Veterinary Medicine …, 2019 - Wiley Online Library… of spacer sequences, using a miniblotter according to the manufacturer's instructions (MapMyGenome) … The spoligotyping was performed on 35 M. bovis isolates and six types of … The other municipalities presented only one spoligotype each, Manacapuru (SB0822), Itacoatiara …

 

MOLECULAR TYPING OF MYCOBACTERIUM KANSASII—A GLOBAL PERSPECTIVE

Z Bakuła, F Arias, E Bachiyska, E Borroni… - Инфекция и …, 2019 - iimmun.ru… cially available kit (Mapmygenome India Ltd., Madhapur, India) according to the manufacturer's protocol … Spoligotypes were obtained for 127 (97.8%) isolates … When spoligotyping and MIRU-VNTR typing was used in com- bination, only tw o, out of 130 isolates, clustered both in …  Cited by 1 Related articles 

 

[HTML] Occurrence of disputed rpoB mutations among Mycobacterium tuberculosis isolates phenotypically susceptible to rifampicin in a country with a low incidence …

NM Al-Mutairi, S Ahmad… - BMC infectious …, 2019 - bmcinfectdis.biomedcentral.com

… Molecular detection of resistance for ethambutol and pyrazinamide and fingerprinting by spoligotyping were also performed for isolates with an rpoB mutation. Results … Spoligotyping showed that the isolates belonged to different M. tuberculosis lineages. Conclusions …  Cited by 5 Related articles All 8 versions 

 

Usefulness of 3'- 5' IS6110-RFLP genotyping and spoligotyping of Mycobacterium tuberculosis isolated in a tertiary hospital: a retrospective study detecting …

SM Almeida, AC Malaspina, CQF Leite… - Revista do Instituto de …, 2019 - SciELO Brasil

… from Ocimum Biosolutions Ltda (Hyderabad, India). An overestimation of the number of

potentially related isolates is a possibility, as the discriminatory power of spoligotyping is lower …

 

Whole-genome sequencing of two extensively drug-resistant Mycobacterium tuberculosis isolates from India

SB Rufai, S Singh - Microbiology Resource Announcements, 2019 - Am Soc Microbiol

… by molecular characterization by application of a spoligotyping technique using a commercial kit (Ocimum Biosolutions, India). The spoligotyping method, which was done per the …

 

Genotype diversity and distribution of Mycobacterium bovis from livestock in a small, high-risk area in northeastern Sicily, Italy.Cinzia Marianelli, Benedetta Amato, Maria Beatrice Boniotti, Maria Vitale, Flavia Pruiti Ciarello, Maria Lodovica Pacciarini, Vincenzo Di Marco Lo Presti.PLoS Negl Trop Dis. 2019 Jul; 13(7): e0007546. Published online 2019 Jul 15. doi: 10.1371/journal.pntd.0007546. PMCID: PMC6658142

Genetic diversity and drug susceptibility profiles of Mycobacterium tuberculosis obtained from  Saint Peter’s TB specialized Hospital, Ethiopia.Delesa Damena, Samuel Tolosa, Milkessa Hailemariam, Aboma Zewude, Adane Worku, Biruk Mekonnen, Temesgen Mohammed, Addisu Admasu, Emile R. Chimusa, Adane  Mihret, Tamrat Abebe, Gobena Ameni.PLoS One. 2019; 14(6): e0218545.  Published online 2019 Jun 24.  doi: 10.1371/journal.pone.0218545. PMCID: PMC6590806

Exploring the “Latin American Mediterranean” family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela. Chyntia Carolina Díaz Acosta, Graciela Russomando, Norma Candia, Viviana Ritacco, Sidra E. G. Vasconcellos, Marcia de Berrêdo Pinho  Moreira, Nilda J. de Romero, Nora Morcillo, Jacobus Henri De Waard, Harrison Magdinier Gomes, Philip Noel Suffys.BMC Microbiol. 2019; 19: 131. Published online 2019 Jun 13. doi: 10.1186/s12866-019-1479-6. PMCID: PMC6567603

Genetic diversity and drug susceptibility patterns of the Mycobacterium tuberculosis complex in Yunnan, China. Ruolan Bai, Shuijing Chi, Xiaofei Li, Xiting Dai, Zhenhua Ji, Miaomiao Jian, Yunfeng  Bi, Feng Wang, Zhe Ding, Lisha Luo, Taigui chen, Aihua Liu, Fukai Bao,Biosci Rep. 2019 May 31; 39(5): BSR20181746.  Pre-published online 2019 Apr 15. Published online 2019 May 31. doi:  10.1042/BSR20181746. PMCID: PMC6542976

Demographic risk factors for extra-pulmonary tuberculosis among adolescents and adults in Saudi ArabiaHawra Al-Ghafli, Bright Varghese, Mushira Enani, Abdulrahman Alrajhi, Sameera Al Johani, Ali  Albarrak, Sahar Althawadi, Noura Elkizzi, Sahal Al Hajoj,PLoS One. 2019; 14(3): e0213846.  Published online 2019 Mar 27. doi: 10.1371/journal.pone.0213846. PMCID: PMC6436801

Tuberculosis Caused by Mycobacterium bovis in a Sheep Flock Colocated with a Tuberculous Dairy Cattle Herd in Central Ethiopia.Benti Deresa Gelalcha, Aboma Zewude, Gobena Ameni,J Vet Med. 2019; 2019: 8315137. Published online 2019 Mar 3. doi: 10.1155/2019/8315137. PMCID: PMC6421010

Active and latent tuberculosis among inmates in La Esperanza prison in Guaduas, Colombia Julio Guerra, Daniel Mogollón, Deccy González, Ricardo Sanchez, Zulma Vanessa Rueda, Carlos A. Parra-López, Martha Isabel Murcia, PLoS One. 2019; 14(1): e0209895. Published online 2019 Jan 25. doi:  10.1371/journal.pone. 0209895. PMCID: PMC6347203

 

2018

Spoligotyping for Identification of Pulmonary Tuberculosis with Type 2 Diabetes Mellitus Profiles in Warangal, TS

S Tanniru, S Asha, M Jojula - Anti-Infective Agents, 2018 - ingentaconnect.com… culosis) and MDR TB (Multi Drug Resistance Tuberculosis) and different Spoligotypes are represented … Molecu- lar methods like Spoligotyping are used to differentiate and identify species of … & molecular identification methods, we found most prevalent Spoligotype pattern which …Related articles

 

Clofazimine drug susceptibility testing for Mycobacterium tuberculosis: the case of using the right diluent

LH Sng, JWL Peh, MTL Kee, RTH Ong, CW Wong… - Pathology, 2018 - Elsevier… The isolates were identified as MTB using the BD MGIT TBc Identification Test (TBcID; Becton Dickinson, USA) and strain typed by spoligotyping (Ocimum Biosolutions, India). DST for clofazimine had been carried out using the MGIT method 10 at a concentration of 0.5 μg/mL …

[HTML] Investigation of a cluster of multi-drug resistant tuberculosis in a high-rise apartment block in Singapore

ZJM Ho, CBE Chee, RTH Ong, LH Sng… - International Journal of …, 2018 - Elsevier… elements [Figure 1]. The isolates were determined in May 2016 to have identical spoligotype(Ocimum Biosolutions, Hyderabad) and 24-loci Mycobacterial Interspersed Repetitive Unit –Variable Number of Tandem Repeat (MIRU-VNTR: Genoscreen, France) profiles (Chee et …Cited by 3 Related articles All 6 versions

 

[PDF] Comparison of PFGE, IS6110-RFLP, and 24-Locus MIRU-VNTR for Molecular Epidemiologic Typing of Mycobacterium tuberculosis Isolates with Known …

S Jeon, N Lim, S Park, M Park… - Journal of microbiology …, 2018 - pdfs.semanticscholar.org… Spoligotyping Spoligotyping was performed to identify the lineages of the isolates used in this …The spoligotypes were classified according to SITVITWEB clade assignments (http://www.pasteru-guadeloupe.fr.  There was no spoligotype match found in the SITVIT database for …  Cited by 1 Related articles All 6 versions 

 

[CITATION] Mycobacterium bovis Infection in a Human in Malaysia

S Suraiya, NI Farakhin - Clinical Microbiology Newsletter, 2018 - Elsevier

[HTML] Molecular clustering of patients with Mycobacterium tuberculosis strains cultured from the diabetic and non-diabetic newly diagnosed TB positive cases

JW Mburu, L Kingwara, M Esther, N Andrew - Journal of Clinical …, 2018 - Elsevier… as described by Kamerbeek et, al. [9]. Spoligotyping was performed using the spoligotype kit (Ocimum bio solutions company, Netherlands) according to the manufacturer's instructions. The direct repeats (DR) were amplified …Related articles All 3 versions

 

Multi-and extensively drug resistant Mycobacterium tuberculosis in South Africa: a molecular analysis of historical isolates

NE Maningi, LT Daum, JD Rodriguez… - Journal of clinical …, 2018 - Am Soc MicrobiolCited by 2 Related articles All 6 versions

 

Annotated genome sequences of 16 lineage 4 Mycobacterium tuberculosis strains from guatemala

JW Saelens, D Lau-Bonilla, A Moller… - Genome …, 2018 - Am Soc Microbiol… 5 Prime GmbH, Germany). Spoligotyping was carried out using the spoligotyping kit and protocol from Isogen Biosolutions (Ocimum Biosolutions Ltd., India), identifying the isolates as lineage 4 strains. Paired-end 50-bp reads …  Cited by 1 Related articles All 5 versions

 

[HTML] Risk factors associated with cluster size of Mycobacterium tuberculosis (Mtb) of different RFLP lineages in Brazil

RL Peres, SA Vinhas… - BMC infectious …, 2018 - bmcinfectdis.biomedcentral.com… Of the isolates spoligotyped, 448 (52%) were classified as LAM and 412 (48%) as … Isolates were also submitted to spoligotyping by a commercial kit (Ocimum Biosolutions Inc., India … a standard protocol [17, 18], allowing the classification of strains into spoligotype-based families …  Cited by 3 Related articles All 9 versions 

 

[HTML] Evidence for Host-Bacterial Co-evolution via Genome Sequence Analysis of 480 Thai Mycobacterium tuberculosis Lineage 1 Isolates

P Palittapongarnpim, P Ajawatanawong… - Scientific …, 2018 - ncbi.nlm.nih.gov… of the single nucleotide variant (SNV) phylogenetic tree and the clades defined by spoligotyping revealed some monophyletic clades representing EAI2_MNL, EAI2_NTM and EAI6_BGD1 spoligotypes. Our work demonstrates that ambiguity in spoligotype assignment could be …  Cited by 5 Related articles All 11 versions

 

[HTML] Genotyping and molecular detection of multidrug-resistant Mycobacterium tuberculosis among tuberculosis lymphadenitis cases in Addis Ababa, Ethiopia

O Zewdie, A Mihret, T Abebe, A Kebede, K Desta… - New microbes and new …, 2018 - Elsevier… Spoligotyping was carried out using a commercially available kit from Ocimum Biosolutions (UK …Spoligotype patterns of each strain were prepared in binary and octal format and entered intothe recently released international spoligotyping database SITVITWEB [12] for …Cited by 2 Related articles All 6 versions

 

[HTML] Impact of Mycobacterium tuberculosis complex lineages as a determinant of disease phenotypes from an immigrant rich moderate tuberculosis burden …

B Varghese, M Enani, A Alrajhi… - Respiratory …, 2018 - respiratory-research.biomedcentral …… according to the manufacturer's instructions. Primary genotyping of all isolates were carried out by using the commercially available spoligotyping assay (Ocimum Biosolutions, Hyderabad, India). The 24 loci based MIRU-VNTR …  Cited by 1 Related articles All 9 versions 

 

Genotypes of Mycobacterium bovis strains isolated from domestic animals and wildlife in Canada in 1985–2015

O Andrievskaia, C Turcotte, G Berlie-Surujballi… - Veterinary …, 2018 - Elsevier… Isolates were spoligotyped as described by Kamerbeek et al … The spacer sequences contained in the DR locus were detected by hybridization onto spoligotyping membranes using … in the DR locus was represented in a binary code, and translated into spoligotype octal codes …  Cited by 4 Related articles All 5 versions

 

[HTML] Association of gyrA and rrs gene mutations detected by MTBDR sl V1 on Mycobacterium tuberculosis strains of diverse genetic background from India

SB Rufai, J Singh, P Kumar, P Mathur, S Singh - Scientific reports, 2018 - nature.com… Spoligotyping was performed using the commercially available kit (Ocimum Biosolutions,Hyderabad … The hybridization patterns obtained in the binary format were transformed to an octal code for assessment with the spoligotype patterns using SITVIT2 database …Cited by 1 Related articles All 6 versions

 

 

[HTML] Bacterial risk factors for treatment failure and relapse among patients with isoniazid resistant tuberculosis

PVK Thai, DTM Ha, NT Hanh… - BMC infectious …, 2018 - bmcinfectdis.biomedcentral.com..Drug resistant tuberculosis (TB) is increasing in prevalence worldwide. Treatment failure and relapse is known to be high for patients with isoniazid resistant TB treated with standard first line regimens. However, risk factors for unfavourable outcomes and the optimal treatment regimen …  Cited by 4 Related articles All 11 versions 

 

 

Genetic Diversity of Mycobacterium tuberculosis Isolates in the Metropolitan Area of Rome

A Cannas, S Camassa, M Sali, O Butera, A Mazzarelli… - Chemotherapy, 2018 - karger.com… All strains were analyzed by the standardized spoligotyping technique as previously described [18] using a commercial kit (Ocimum Biosolutions, India) … Spoligotype patterns were represented as octal codes and entered in the SITVIT2 proprietary database of the …Related articles All 6 versions

 

Comparison of methods and samples used in the diagnosis of childhood PTB and characterization of mycobacterium tuberculosis isolates

AE Ogunbayo - 2018 - scholar.ufs.ac.za… Polymorphism..... 42 2.7.2.2. Spoligotyping ..... 43 2.7.2.3 …3.10.9.1. Spoligotyping ..... 60 3.10.9.2. MIRU … Related articles All 3 versions 

 

[PDF] 8. Molecular typing of Mycobacterium tuberculosis complex isolates

G Skenders, D van Soolingen - Handbook on tuberculosis laboratory …, 2018 - tbonline.info… It is, however, possible to use spoligotyping as a screening method in typing (in the vast … On the other hand, when spoligotype patterns are identical, then no conclusion can be drawn … Protocol: PCR: 1. Reconstitute dry primers Dra and DRb (Ocimum Biosolutions, India) in 2.2 ml …

 

[HTML] Mycobacterium africanum (Lineage 6) shows slower sputum smear conversion on tuberculosis treatment than Mycobacterium tuberculosis (Lineage 4) in …

B Diarra, M Kone, ACG Togo, YDS Sarro, AB Cisse… - PloS one, 2018 - journals.plos.org… or 5M. Spoligotyping was performed on boiled bacterial lysates using a commercially available kit (Ocimum)[22] or by in-house prepared membrane. Data entry from the film was verified by a second assessor. Lineage classification …

 

The effect of combining natural terpenes and antituberculous agents against reference and clinical Mycobacterium tuberculosis strains

E Sieniawska, R Sawicki, M Swatko-Ossor… - Molecules, 2018 - mdpi.com… It was described in most of the common highly fragrant herbs and spices, among which Humulus lupulus, Laurus nobilis, Thymus vulgaris, Cymbopogon schoenanthus or Ocimum basilicum contain high levels of this compound [17] … 2. Results. 2.1. Spoligotyping …  Cited by 7 Related articles All 8 versions 

 

Molecular Characterization of Drug-Resistant Mycobacterium Tuberculosis and Evaluation of the Performance of Genexpert Mtb/Rif® Assay Using Urine Specimen to …

E Abera - 2018 - 213.55.95.56… 113 Figure 5.1: Radial UPGMA tree based on the copy numbers of MIRU-VNTR 24-loci and Spoligotyping, and it shows the pattern of drug resitance lineage and clustering of each genotype … 24 MIRU-VNTR and spoligotype. Data were analyzed using SPSS version 23 …

 

Impact of Mycobacterium tuberculosis complex lineages as a determinant of disease phenotypes from an immigrant rich moderate tuberculosis burden country, Bright Varghese, Mushira Enani, Abdulrahman Alrajhi, Sameera Al Johani, Ali Albarak, Sahar Althawadi, Noura Elkhizzi, Hawra AlGhafli, Mohammed Shoukri, Sahal Al - Hajoj, Respir Res. 2018; 19: 259. Published online 2018 Dec 27. doi: 10.1186/s12931-018-0966-x,PMCID: PMC6307224

Mycobacterium africanum (Lineage 6) shows slower sputum smear conversion on tuberculosis treatment than Mycobacterium tuberculosis (Lineage 4) in Bamako, Mali.Bassirou Diarra, Mahamadou Kone, Antieme Combo Georges Togo, Yeya dit Sadio Sarro, Aissata Boubakar Cisse, Amadou Somboro, Boureima Degoga, Mohamed Tolofoudie, Bourahima Kone, Moumine Sanogo, Bocar Baya, Ousmane Kodio, Mamoudou Maiga, Michael Belson, Susan Orsega, Meryam Krit, Sounkalo Dao, Ibrahim Izétiegouma Maiga, Robert L. Murphy, Leen Rigouts, Seydou Doumbia, Souleymane Diallo, Bouke Catherine de Jong PLoS One. 2018; 13(12): e0208603. Published online 2018 Dec 12. doi: 10.1371/journal.pone.0208603. PMCID: PMC6291124

Spoligotype‐based population structure of Mycobacterium tuberculosis in the Jimma Zone, southwest Ethiopia. Gemeda Abebe, Ketema Abdissa, Kedir Abdella, Mulualem Tadesse, Adane Worku, Gobena Ameni, Microbiology open. 2019 Jun; 8(6): e00744. Published online 2018 Oct 15. doi: 10.1002/mbo3.744. PMCID: PMC6562125

Evidence for Host-Bacterial Co-evolution via Genome Sequence Analysis of 480 Thai Mycobacterium tuberculosis Lineage 1 Isolates. Prasit Palittapongarnpim, Pravech Ajawatanawong, Wasna Viratyosin, Nat Smittipat, Areeya Disratthakit, Surakameth Mahasirimongkol, Hideki Yanai, Norio Yamada, Supalert Nedsuwan, Worarat Imasanguan, Pacharee Kantipong, Boonchai Chaiyasirinroje, Jiraporn Wongyai, Licht Toyo-oka, Jody Phelan, Julian Parkhill, Taane G. Clark, Martin L. Hibberd, Wuthiwat Ruengchai, Panawun Palittapongarnpim, Tada Juthayothin, Sissades Tongsima, Katsushi Tokunaga Sci Rep. 2018; 8: 11597. Published online 2018 Aug 2. doi: 10.1038/s41598-018-29986-3. PMCID: PMC6072702

Association of gyrA and rrs gene mutations detected by MTBDRsl V1 on Mycobacterium tuberculosis strains of diverse genetic background from India.Syed Beenish Rufai, Jitendra Singh, Parveen Kumar, Purva Mathur, Sarman Singh. Sci Rep. 2018; 8: 9295. Published online 2018 Jun 18. doi: 10.1038/s41598-018-27299-z. PMCID: PMC6006251

Multi- and Extensively Drug Resistant Mycobacterium tuberculosis in South Africa: a Molecular Analysis of Historical Isolates. Nontuthuko E. Maningi, Luke T. Daum, John D. Rodriguez, Halima M. Said, Remco P. H. Peters, John Osei Sekyere, Gerald W. Fischer, James P. Chambers, P. Bernard Fourie J Clin Microbiol. 2018 May; 56(5): e01214-17.  Pre-published online 2018 Mar 7. Published online 2018 Apr 25. doi: 10.1128/JCM. 01214-17.PMCID: PMC5925707

Annotated Genome Sequences of 16 Lineage 4 Mycobacterium tuberculosis Strains from Guatemal Joseph W. Saelens, Dalia Lau-Bonilla, Anneliese Moller, Ana M. Xet-Mull, Narda Medina, Brenda Guzmán, Maylena Calderón, Raúl Herrera, Jason E. Stout, Eduardo Arathoon, Blanca Samayoa, David M. Tobin Genome Announc. 2018 Feb; 6(7): e00024-18. Published online 2018 Feb 15. doi: 10.1128/genomeA.00024-18. PMCID: PMC5814500

Risk factors associated with cluster size of Mycobacterium tuberculosis (Mtb) of different RFLP lineages in Brazil. Renata Lyrio Peres, Solange Alves Vinhas, Fabíola Karla Correa Ribeiro, Moisés Palaci, Thiago Nascimento do Prado, Bárbara Reis-Santos, Eliana Zandonade, Philip Noel Suffys, Jonathan E. Golub, Lee W. Riley, Ethel Leonor Maciel,BMC Infect Dis. 2018; 18: 71. Published online 2018 Feb 8. doi: 10.1186/s12879 018-2969-0. PMCID: PMC5806441

The Effect of Combining Natural Terpenes and Antituberculous Agents against Reference and Clinical Mycobacterium tuberculosis Strains,Elwira Sieniawska, Rafal Sawicki, Marta Swatko-Ossor, Agnieszka Napiorkowska, Agata Przekora, Grazyna Ginalska, Ewa Augustynowicz-Kopec Molecules. 2018 Jan; 23(1): 176.  Published online 2018 Jan 15. doi: 10.3390/molecules23010176.PMCID: PMC6017631

Genetic diversity and distribution of Mycobacterium tuberculosis genotypes in Limpopo, South Africa.N. T. C. Maguga-Phasha, N. S. Munyai, F. Mashinya, M. E. Makgatho, E. F. Mbajiorgu .BMC Infect Dis. 2017; 17: 764.  Published online 2017 Dec 12. doi: 10.1186/s12879-017-2881-z PMCID: PMC5727936

Genotyping and molecular detection of multidrug-resistant Mycobacterium tuberculosis among tuberculosis lymphadenitis cases in Addis Ababa, Ethiopia.O. Zewdie, A. Mihret, T. Abebe, A. Kebede, K. Desta, A. Worku, G. Ameni,New Microbes New Infect. 2018 Jan; 21: 36–41.  Published online 2017.Oct 24.oi: 10.1016/j.nmni.2017.10.009.PMCID: PMC5901530

PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping. B. Molina-Moya, A. Lacoma, N. García-Sierra, S. Blanco, L. Haba, S. Samper, J. Ruiz-Manzano, C. Prat, C. Arnold, J. Domínguez,Sci Rep. 2017; 7: 6777.  Published online 2017 Jul 28. doi: 10.1038/s41598-017-06760-5,PMCID: PMC5533701

First Insight into the Fluoroquinolone and Aminoglycoside Resistance of Multidrug-Resistant Mycobacterium tuberculosis in Saudi Arabia,Bright Varghese, Sahal Al-Hajoj,Am J Trop Med Hyg. 2017 May 3; 96(5): 1066–1070.  doi: 10.4269/ajtmh.16-0579Correction in: Am J Trop Med Hyg. 2018 Feb; 98(2): 634. PMCID: PMC5417196

2017

 

Strains of Mycobacterium tuberculosis Transmitting Infection in Brazilian Households and Those Associated with Community Transmission of Tuberculosis.Solange Alves Vinhas, Edward C. Jones-López, Rodrigo Ribeiro Rodrigues, Mary Gaeddert, Renata Lyrio Peres, Patricia Marques-Rodrigues, Paola Poloni Lobo de Aguiar, Laura Forsberg White, David Alland,Padmini Salgame, David Hom, Jerrold J. Ellner, Reynaldo Dietze, Lauren F. Collins, Elena Shashkina, Barry Kreiswirth, Moisés Palaci .Tuberculosis (Edinb) Author manuscript; available in PMC 2018 May 1.Published in final edited form as: Tuberculosis (Edinb). 2017 May; 104: 79–86. Published online 2017 Mar 16.  doi: 10.1016/j.tube.2017.03.003.PMCID: PMC5716464

 

Molecular typing of Mycobacterium tuberculosis complex isolated from pulmonary tuberculosis patients in central Ethiopia.Zufan Bedewi, Adane Worku, Yalemtsehay Mekonnen, Getnet Yimer, Girmay Medhin, Gezahegne Mamo, Rembert Pieper, Gobena Ameni,BMC Infect Dis. 2017; 17: 184.  Published online 2017 Mar 1. doi: 10.1186/s12879-017-2267-2.PMCID: PMC5333391

 

[HTML] Trends in spoligotype patterns of Mycobacterium tuberculosis strains in central India

P Desikan, DS Chauhan, N Panwalkar… - Indian journal of medical …, 2017 - ijmm.org… Table 1: Year wise occurrence of spoligotype lineages over the period of five years Click here to view … Comparison of spoligotyping, mycobacterial interspersed repetitive units typing and IS6110-RFLP in a study of genotypic … Predominant tuberculosis spoligotypes, Delhi, India …Cited by 1 Related articles All 4 versions

 

[HTML] Molecular typing of Mycobacterium tuberculosis complex isolated from pulmonary tuberculosis patients in central Ethiopia

Z Bedewi, A Worku, Y Mekonnen… - BMC infectious …, 2017 - bmcinfectdis.biomedcentral.com… Isolates that were positive for M. tuberculosis by RD9 PCR were further characterized by spoligotyping following the procedure described by Kamerbeeket al [17] and by observing the instructions of the spoligotype kit supplier (Ocimum Biosolutions Company, Iisselstein, and the …

 

 

First Insight into the Fluoroquinolone and Aminoglycoside Resistance of Multidrug-Resistant Mycobacterium tuberculosis in Saudi Arabia

B Varghese, S Al-Hajoj - The American journal of tropical medicine and …, 2017 - ASTMH… Genotyping of MDR-TB isolates and data analysis. All the isolates reported with resistance to either amino- glycosides or fluoroquinolone were subjected to spoligotyping (Ocimum Biosolutions, Hyderabad, India) and 24-loci-based …

Cited by 1 Related articles All 4 versions

 

 

[HTML] Genetic diversity and distribution of Mycobacterium tuberculosis genotypes in Limpopo, South Africa

NTC Maguga-Phasha… - BMC infectious …, 2017 - bmcinfectdis.biomedcentral.com… They were spoligotyped and data analysed using the international spoligotyping database 4 (SpolDB4). Results … The results of spoligotyping analysis and a sample Spoligo pattern are presented in Table 1 and Fig. 2, respectively. Table 1 … Spoligotype family. Total isolates …  Cited by 2 Related articles All 7 versions 

 

Genetic diversity of Mycobacterium tuberculosis from Pará, Brazil, reveals a higher frequency of ancestral strains than previously reported in South America

EC Conceição, N Rastogi, D Couvin, ML Lopes… - Infection, Genetics and …, 2017 - Elsevier… In this database, Spoligotype International Type (SIT) designates patterns shared by two or … used the SpolSimilaritySearch tool to highlight the specificity of spoligotyping signatures in … Distribution of patient demographics, drug-susceptibility testing results and major spoligotypes …Cited by 5 Related articles All 8 versions

 

[HTML] Genetic diversity of Mycobacterium tuberculosis and transmission associated with first-line drug resistance: a first analysis in Jalisco, Mexico

G Lopez-Avalos, G Gonzalez-Palomar… - Journal of global …, 2017 - Elsevier… [19] using a Spoligotyping Diagnostic Kit with primers and controls (Ocimum Biosolutions LCC, Indianapolis, IN) following the manufacturer's instructions … In this database, spoligotype international type (SIT) designates a spoligotyping pattern shared by two or more patient …Cited by 6 Related articles All 7 versions

 

Cross-contamination of Mycobacterium tuberculosis at the National Rference Laboratory of Tuberculosis of Cuba

MR Martínez Romero, M Sardiña Aragón… - Revista Cubana de …, 2017 - medigraphic.com… A los aislamientos se les extrajo el ADN según lo descrito por van Soolingen y otros.8 Se empleóel sistema Spoligotyping Kit IM9701 (Ocimum Biosolutions Ltd, Hyderabad, India), de acuerdocon las instrucciones del fabricante y lo descrito por Kamerbeek y otros9 Los …  Related articles All 2 versions 

 

[HTML] PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping

B Molina-Moya, A Lacoma, N García-Sierra, S Blanco… - Scientific reports, 2017 - nature.com… Spoligotyping was performed using the spoligokit (Ocimum Biosolutions, Hyderabad, India) following the manufacturer's instructions … Spoligotyping International Types (SIT) were assigned according to spoligotype pattern signatures provided in SITVITWEB … Cited by 1 Related articles All 7 versions

 

Strains of Mycobacterium tuberculosis transmitting infection in Brazilian households and those associated with community transmission of tuberculosis

SA Vinhas, EC Jones-López, RR Rodrigues… - Tuberculosis, 2017 - Elsevier… frequencies of MTB isolates clustered assorted by RFLP and Spoligotyping in Vitoria … groups, the isolates were compared with those classified as Spoligotype International Type … Genotypes (IS6110 RFLP patterns and spoligotypes) were grouped according to three approaches … Cited by 2 Related articles All 8 versions

 

Genomic, Genetic, and Functional Interrogation of Mycobacterium tuberculosis Outbreak Strains

JW Saelens - 2017 - search.proquest.com… 45 2.6.3 Spoligotyping ..... 45 … xi List of Tables Table 1: Spoligotypes and patient risk factors for the 11 identified Beijing isolates from the Clínica Familiar “Luis Angel García” (CFLAG) and its associated public hospital …

 

Influência de fatores de risco do paciente e de características de Mycobacterium tuberculosis no desfecho dos casos novos de tuberculose pulmonar tratados com …

MC Scheffer - 2017 - repositorio.ufsc.br… de todos os spoligotypes encontrados nos isolados de M. tuberculosis incluídos no estudo, classificação segundo SITVIT-WEB..... 87 Figura 22- Distribuição das famílias e subfamílias segundo genotipagem por metodologia de Spoligotyping de isolados de M …  Related articles All 2 versions 

 

EPIDEMIOLOGY OF TUBERCULOSIS IN CAMELS SLAUGHTERED AT AKAKI ABATTOIR, CENTRAL ETHIOPIA

J YASMIN - 2017 - 213.55.95.56… while the rest were non-tuberculous mycobacteria (NTM). Spoligotyping of the 39 isolates indicated that majority of the isolates (94.9%) identified as orphan strains with no shared spoligotype international type (SIT) in the Spoligotype database while two …  Related articles 

 

[PDF] Characterization of efflux pumps genes involved in second-line drug resistance of tuberculosis

LA Malinga - 2017 - pdfs.semanticscholar.orgPage 1. Characterization of efflux pumps genes involved in second-line drug resistance of tuberculosis Lesibana Anthony Malinga © University of Pretoria Page 2. CHARACTERIZATION OF EFFLUX PUMPS GENES INVOLVED IN …

 

2016

 

Determination of genotypic varieties and genotyping of multiple drug-resistant tuberculosis by the RFLP and spoligotyping methods

T Karsligil, A BAYRAM, F EKŞİ - Turkish journal of medical …, 2016 - journals.tubitak.gov.tr The spoligotype patterns were encoded in triplet codes, and the types were identified (Figure 1). The obtained results were compared with the international spoligotyping database, and the spoligotypes were detected (15) …

  Related articles All 9 versions 

 

Role of gyrB mutations in pre-extensively and extensively drug-resistant tuberculosis in Thai clinical isolates

A Disratthakit, T Prammananan… - Antimicrobial agents …, 2016 - Am Soc Microbiol Cited by 10 Related articles All 7 versions

 

 

Secretome profiling of highly virulent Mycobacterium bovis 04-303 strain reveals higher abundance of virulence-associated proteins

F Vargas-Romero, G Mendoza-Hernández… - Microbial …, 2016 - Elsevier… From the M. bovis strains, 19 different spoligotyping patterns were observed among the 27 … M.bovis molecular typing database (Table 1). The most frequently observed spoligotypes were the … Only the 444 strain presented the spoligotype SB0140 and were used for comparison … Cited by 5 Related articles All 6 versions

 

[HTML] Evolutionary History and Ongoing Transmission of Phylogenetic Sublineages of Mycobacterium tuberculosis Beijing Genotype in China

Q Yin, H Liu, W Jiao, Q Li, R Han, J Tian, Z Liu… - Scientific reports, 2016 - nature.com… IS6110-RFLP or 24-VNTR) or slowly evolving phylogenetic markers (eg spoligotypes, RD and …in M. tuberculosis prevent from making any phylogenetic inference from the spoligotyping data of … For example, spoligotype SIT190 with deleted signal #40 was found in strains of both …Cited by 15 Related articles All 9 versions

 

[HTML] Genetic diversity of Mycobacterium tuberculosis complex strains isolated from patients with pulmonary tuberculosis in Anambra State, Nigeria

GN Uzoewulu, L Lawson, IS Nnanna, N Rastogi… - International journal of …, 2016 - Elsevier… The identified spoligotypes and five-loci ETR patterns were analyzed using the BioNumerics … result of the comparison of spoligotyping versus VNTRs demonstrated that spoligotyping analysis alone …with five-loci ETRs; for example, isolates with identical spoligotype pattern SIT61 …Cited by 8 Related articles All 15 versions

 

[HTML] Genetic diversity of Mycobacterium tuberculosis isolates from central India

P Desikan, DS Chauhan, P Sharma… - The Indian journal of …, 2016 - ncbi.nlm.nih.gov… Spoligotyping was carried out by using a commercial kit (Ocimum Biosolutions, Hyderabad) at …SITVIT2 database of Institute Pasteur de Guadeloupe 5 to locate families of spoligotypes … of comparison, the database consisted of a total of 7105 spoligotype patterns (corresponding …Cited by 4 Related articles All 6 versions

 

First insight into the molecular epidemiology of Mycobacterium tuberculosis in Santa Catarina,southern Brazil

CL Nogueira, RI Prim, SG Senna, DB Rovaris… - Tuberculosis, 2016 - Elsevier… Spoligotyping was performed by using commercially available membranes (Ocimum Biosolutions,Hyderabad … In this database, Spoligotype International Type (SIT) designates patterns shared by … The evolutionary relationships among all the observed spoligotypes were studied …Cited by 11 Related articles All 9 versions

 

[HTML] Analysis of Mycobacterium tuberculosis genotypic lineage distribution in Chile and neighboring countries

J Lagos, D Couvin, L Arata, J Tognarelli, C Aguayo… - PloS one, 2016 - journals.plos.org… The Fig 1B shows the spoligotyping-based MST; it is evident that LAM and T lineages … LAM9 cluster was the largest node evolved from SIT53/T1 and multiple spoligotypes were observed to be rising from it, eg, SIT33/LAM3, while the second largest spoligotype pattern SIT50/H3 … Cited by 4 Related articles All 13 versions 

 

[PDF] Molecular characterization of Mycobacterium tuberculosis complex in Gambella region, Southwest Ethiopia

G Asebe, A Worku, A Zewude, K Tafess… - African Journal of …, 2016 - researchgate.net… Figure 3. Spoligotype patterns of isolates obtained from Gambella … Page 7. 1092 Afr. J. Microbiol. Res. Table 3. Proportion of spoligotyping pattern of the strains with different variables … Table 4. Distribution of spoligotypes and their report status from Ethiopia … Cited by 4 Related articles All 3 versions 

 

[HTML] A novel molecular strategy for surveillance of multidrug resistant tuberculosis in high burden settings

HM Said, N Kushner, SV Omar, AW Dreyer… - PloS one, 2016 - journals.plos.org… Genotyping. Spoligotyping was done using a commercial kit (Ocimum Biosolutions, India) according to the manufacturer's instructions [20]; spoligotypes were uploaded as binary codes in the SpolDB4 database for SIT (Spoligotype International Type) assignment …

 

[PDF] Molecular genotypes of Mycobacterium tuberculosis strains circulating in Dakar, Senegal

AB Diallo, OG Walbang, M Camara, S Lo… - African Journal of …, 2016 - researchgate.net… Spoligotyping and assignment of mycobacterial lineage and families … consecutive smear- positive TB patients out of which 15 (6.8%) did not yield a spoligotype pattern and … remaining 203 M. tuberculosis complex isolates, we identified a total of 59 different spoligotypes patterns …  Cited by 2 Related articles All 2 versions 

 

Molecular typing of mycobacteria isolated from tuberculous lymphadenitis cases in Addis Ababa, Ethiopia

O Zewdie, A Mihret, G Ameni, A Worku… - … of Tuberculosis and …, 2016 - ingentaconnect.com… Spoligotyping was used as the primary tool for typing MTC strains because of its ease of … SIT =spoligotype international type; CAS = Central Asian; EA = Euro-American; IO = Indo-Oceanic; LAM … A long descendant branch of spoligotypes belonged to the dominant 'ill-defined' T …Cited by 5 Related articles All 5 versions

 

[HTML] Whole-genome sequencing analysis of serially isolated multi-drug and extensively drug resistant Mycobacterium tuberculosis from Thai patients

K Faksri, JH Tan, A Disratthakit, E Xia… - PloS one, 2016 - journals.plos.org… Classical genotyping. Spoligotyping was performed according to the manufacturer's instructions (Ocimum Biosolutions, India) as previously described [19]. Twenty four loci MIRU-VNTR typing was performed according to the …

 

Latin-American-Mediterranean lineage of Mycobacterium tuberculosis: Human traces across pathogen's phylogeography

I Mokrousov, A Vyazovaya, T Iwamoto, Y Skiba… - Molecular phylogenetics …, 2016 - Elsevier… Spoligotyping was performed with in-house made membranes or using commercial kits (Ocimum Biosolutions … Profile #6 includes strains of 7 different spoligotypes from distant parts of Russia (Northwest … strains with SIT1321 and SIT64 that do not bear this spoligotype signature …

 

[HTML] Molecular typing of Mycobacterium Bovis from cattle reared in Midwest Brazil

RCT Carvalho, SEG Vasconcellos, M de Azevedo Issa… - PloS one, 2016 - journals.plos.org… The geographic distribution of the spoligotypes is presented in Fig 1 … bovis spoligotype found in strains isolated at municipalities of Midwest Brazil. https://doi.org/10.1371/journal.pone.0162459. g001 … by spoligotyping method. https://doi.org/10.1371/journal.pone.0162459.t001 …

 

[HTML] Chromosomal rearrangements and protein globularity changes in Mycobacterium tuberculosis isolates from cerebrospinal fluid

SH Saw, JL Tan, XY Chan, KG Chan, YF Ngeow - PeerJ, 2016 - peerj.com… The genotypes identified using the spoligotyping kit from Ocimum Biosolutions (Hyderabad, India) were Beijing ST1 (UM-CSF01, UM-CSF05, UM-CSF08, UM-CSF09 and UM-CSF17), EAI_IND (UM-CSF06), Unknown (UM-CSF04) and H3 ST50 (UM-CSF15) …  Cited by 3 Related articles All 11 versions 

 

[HTML] Contaminación cruzada por Mycobacterium tuberculosis en el Laboratorio Nacional de Referencia de Tuberculosis de Cuba

MR Martínez Romero, M Sardiña Aragón… - Revista Cubana de …, 2016 - scielo.sld.cu… 8 Se empleó el sistema Spoligotyping Kit IM9701 (Ocimum Biosolutions Ltd, Hyderabad, India), de acuerdo con las instrucciones del fabricante y lo descrito por Kamerbeek y otros 9 Los patrones obtenidos se compararon con la base de datos internacional SITVIT2 "en línea" …  Cited by 1 Related articles All 6 versions 

 

Mycobacterium Tuberculosis In Central Ethiopia: Molecular Epidemiology, Drug Sensitivity Patterns, and Evaluation of the Genotype Mtbdrplus Assay for the Detection …

Z Bedewi - 2016 - 213.55.95.56… The isolates were analyzed using region of difference (RD) 9-based polymerase chain reaction (PCR) and spoligotyping … Of these 90 spoligotypes, 32 were clustered consisting of 223 (79.3%) isolates while 58 had only a single isolate each …

  

The epidemiology of tuberculosis in cattle and humans living in the wildlife-livestock-human interface in the rural Mnisi community Mpumalanga province South Africa

J Musoke - 2016 - repository.up.ac.za… 85 5.3.5.2 Polymerase Chain Reaction (PCR) .... 86 5.3.5.3 Spoligotyping ..... 86 5.3.5.4 Mycobacterial interspersed repetitive-unit-variable number tandem ..... 87 …

 

[HTML] Molecular analysis of genetic mutations among cross-resistant second-line injectable drugs reveals a new resistant mutation in Mycobacterium tuberculosis

L Malinga, J Brand, S Olorunju, A Stoltz… - … and infectious disease, 2016 - Elsevier… Spoligotyping. The PCR products were amplified using primers purchased from manufacturer (Ocimum Biosciences, India) and the procedure was performed as … Amersham Biosciences) and by exposure to X-ray film (HyperfilmECL, Amersham).The spoligotypes were reported …

Association of Mycobacterium tuberculosis genotypes and treatment outcome in pulmonary tuberculosis patients in Tshwane metropolitan area

SR Matukane - 2016 - repository.up.ac.za

… 34 3.3.2 Data collection 35 3.3.3 DNA extraction 35 3.3.4 Spoligotyping 35 3.3.5 MIRU-VNTR typing 36 Page 8 … 38 4.1 Results 38 4.1.1 Spoligotyping and 24-loci MIRU-VNTR typing results 38 4.1.2 Association of genotypes and drug resistance with treatment outcome 39 …  Related articles All 3 versions 

 

Wild-type minimal inhibitory concentration distributions of secondline drugs in mycobacterium tubercolosis complex clinical isolated in relation to recommended critical …

NM Seloma - 2016 - ulspace.ul.ac.za… SpolDB4 - is an international spoligotyping database that defines M. tuberculosis complex genetic polymorphism, isolates sharing identical spoligotype pattern are defined according to SIT Spoligotype International Type) isolates with single spoligotype patterns are defined as …  Related articles All 2 versions 

 

Development and validation of a molecular assay and evaluation of the GeneXpert® MTB/RIF assay for the rapid detection of genital tuberculosis

MC Sokhela - 2016 - scholar.ufs.ac.za… Figure 3.1 Prevalence of spoligotypes in South Africa ..... 56 Figure 3.2 MIRU-VNTR 24 loci copy number ..... 62Figure 3.3 MIRU-VNTR and Spoligotyping results …

 

[HTML] Tuberculosis por Mycobacterium bovis en trabajadores de fincas en saneamiento para tuberculosis bovina, de Antioquia, Boyacá y Cundinamarca

AF Leal-Bohórquez, CM Castro-Osorio… - Revista de Salud …, 2016 - SciELO Public Health… Kremer K, Bunschoten A, Schouls L, Van Soolingen D, Van Embden J. Laboratory Spoligotyping, a PCR-based method to simultaneously detect … el producto de la reacción en cadena de la polimerasa (RCP) se hibridó en una membrana comercial (Ocimum Biosolutions India …

 

[PDF] Usefulness of molecular methods for detecting drug resistance and genotyping in mycobacterium tuberculosis

BM Moya - 2016 - core.ac.uk… RFLP) .... 43 1.7.2. Spoligotyping ..... 44 … RFLP) .... 64 4.4.2. Spoligotyping ..... 65 …  Related articles All 3 versions

 

Usefulness of molecular methods for detecting drug resistance and genotyping in mycobacterium tuberculosis

B Molina Moya - 2016 - ddd.uab.cat… RFLP) .... 43 1.7.2. Spoligotyping ..... 44 … RFLP) .... 64 4.4.2. Spoligotyping ..... 65 …  Related articles 

 

 

[HTML] Tuberculosis caused by Mycobacterium bovis in workers of bovine tuberculosis sanitation farms in Antioquia, Boyacá and Cundinamarca

AF Leal-Bohórquez, CM Castro-Osorio… - Revista de Salud …, 2016 - scielo.org.co… La metodología de Spoligotyping se realizó según lo descrito por Kremer en el 2002 … por 30 segundos y una extensión final a 72°C por 5 minutos; el producto de la reacción en cadena de la polimerasa (RCP) se hibridó en una membrana comercial (Ocimum Biosolutions India … Related articles All 3 versions 

 

[BOOK] Clinical and pathological characteristics of the Uganda genotype of Mycobacterium tuberculosis

D Wamala - 2016 - openarchive.ki.se… A study of the evolutionary relationships and worldwide distribution of the spoligotypes of Mtb isolates from Ugandan patients with tuberculous lymphadenitis indicated an ongoing evolution of the Uganda genotype, with Uganda at the center of this evolution (Paper II) …  Related articles All 3 versions 

 

Genotipificación de Mycobacterium tuberculosis en aislados clínicos obtenidos de pacientes VIH positivos de los hospitales Simón Bolívar y Santa Clara de Bogotá

MY Beltrán León - 2016 - bdigital.unal.edu.co… A total of 49 isolates of Mycobacterium tuberculosis were genotyped by spoligotyping and MIRU VNTR loci 24 … Spoligotype analysis showed 15 patterns, of which 3/49 (6,1%) were orphans and 45/49 (91,8%) belonged to the Euro American lineage …  Cited by 4 Related articles 

 

Genotipos de Mycobacterium tuberculosis prevalentes en Argentina y su relación con mutaciones de resistencia a isoniacida

J Monteserin - 2016 - ridaa.unq.edu.ar

… La genotipificación se realizó mediante spoligotyping, y en casos selectos, por MIRU-VNTR24. El análisis de clustering se realizó mediante RFLP IS6110 … como spoligotyping y MIRUs-VNTR (Mycobacterial interspersed repetitive units-variable …

Molecular typing of mycobacteria isolated from tuberculous lymphadenitis cases in Addis Ababa, Ethiopia.O. Zewdie, A. Mihret, G. Ameni, A. Worku, T. Gemechu, T. Abebe,Int J Tuberc Lung Dis.Author manuscript; available in PMC 2017 Nov 1.Published in final edited form as: Int J Tuberc Lung.Dis. 2016 Nov; 20(11): 1529–1534.  doi: 10.5588/ijtld.15.1023.PMCID: PMC5665165

 Evolutionary History and Ongoing Transmission of Phylogenetic Sublineages of Mycobacterium tuberculosis Beijing Genotype in China,Qing-qin Yin, Hai-can Liu, Wei-wei Jiao, Qin-jing Li, Rui Han, Jian-ling Tian, Zhi-guang Liu, Xiu-qin Zhao, Ying-jia Li, Kang-lin Wan, A-dong Shen, IgorMokrousov,Sci Rep. 2016; 6: 34353.  Published online 2016 Sep 29. doi: 10.1038/srep34353.PMCID: PMC5041183

Chromosomal rearrangements and protein globularity changes in Mycobacterium tuberculosis isolates from cerebrospinal fluid.Seow Hoon Saw, Joon Liang Tan, Xin Yue Chan, Kok Gan Chan, Yun Fong Ngeow.PeerJ. 2016; 4: e2484.  Published online 2016 Sep 21. doi: 10.7717/peerj.2484.PMCID: PMC5036109

Molecular Typing of Mycobacterium bovis from Cattle Reared in Midwest Brazil,Ricardo César Tavares Carvalho, Sidra Ezidio Gonçalves Vasconcellos, Marina de Azevedo Issa, Paulo Martins Soares Filho, Pedro Moacyr Pinto Coelho Mota, Flábio Ribeiro de Araújo, Ana Carolina da Silva Carvalho, Harrison Magdinier Gomes, Philip Noel Suffys, Eduardo Eustáquio de Souza Figueiredo, Vânia Margaret.Flosi Paschoalin.PLoS One. 2016; 11(9): e0162459.  Published online 2016 Sep 15. doi: 10.1371/journal.pone.0162459. PMCID: PMC5024986

Role of gyrB Mutations in Pre-extensively and Extensively Drug-Resistant Tuberculosis in Thai  Clinical Isolates.Areeya Disratthakit, Therdsak Prammananan, Chanwit Tribuddharat, Iyarit  Thaipisuttikul, Norio Doi, Manoon Leechawengwongs, Angkana Chaiprasert,Antimicrob Agents Chemother. 2016 Sep; 60(9): 5189–5197.  Prepublished online 2016 Jun 13. Published online 2016 Aug 22. doi: 10.1128/AAC.00539-16.PMCID: PMC4997844

Analysis of Mycobacterium tuberculosis Genotypic Lineage Distribution in Chile and Neighboring Countries.Jaime Lagos, David Couvin, Loredana Arata, Javier Tognarelli, Carolina Aguayo, TamaraLeiva, Fabiola Arias, Juan Carlos Hormazabal, Nalin Rastogi, Jorge Fernández,PLoS One. 2016; 11(8): e0160434.  Published online 2016 Aug 12. doi: 10.1371/journal.pone.0160434.PMCID: PMC4982630

Whole-Genome Sequencing Analysis of Serially Isolated Multi-Drug and Extensively Drug Resistant Mycobacterium tuberculosis from Thai Patients.Kiatichai Faksri, Jun Hao Tan, Areeya Disratthakit, Eryu Xia, Therdsak Prammananan, Prapat Suriyaphol, Chiea Chuen Khor, Yik-Ying Teo, Rick Twee-Hee Ong, Angkana Chaiprasert.PLoS One. 2016; 11(8): e0160992.  Published online 2016 Aug 12. doi: 10.1371/journal.pone.0160992.PMCID: PMC4982626

Genetic diversity of Mycobacterium tuberculosis isolates from central India.Prabha Desikan, D.S. Chauhan, Pragya Sharma, Nikita Panwalkar, Manju Chourey, Mohan Lal Patidar, Priyanka Yadav, V. Chandrasekaran, B.S. Ohri.Indian J Med Res. 2016 Apr; 143(4): 481–486.doi: 10.4103/0971-5916.184287.PMCID: PMC4928555

Association between human leukocyte antigen class II and pulmonary tuberculosis due to mycobacterium tuberculosis in Uganda.Dan Wamala, Helen Koyokoyo Buteme, Samuel Kirimunda, Gunilla Kallenius, Moses Joloba.BMC Infect Dis. 2016; 16: 23.  Published online 2016 Jan 23.doi: 10.1186/s12879-016-1346-0.PMCID: PMC4724396

A Novel Molecular Strategy for Surveillance of Multidrug Resistant Tuberculosis in High BurdenettingsHalima M. Said, Nicole Kushner, Shaheed V. Omar, Andries W. Dreyer, Hendrik Koornhof, Linda Erasmus,Yasmin Gardee, Ivy Rukasha, Elena Shashkina, Natalie Beylis, Gilla Kaplan, Dorothy Fallows,Nazir A. Ismail.PLoS One. 2016; 11(1): e0146106. Published online 2016 Jan 11.doi: 10.1371/journal.pone.0146106.PMCID: PMC4713439

 

2015

Genetic Diversity of Mycobacterium tuberculosis Isolates from Assam, India: Dominance of Beijing Family and Discovery of Two New Clades Related to CAS1_Delhi and EAI Family Based on Spoligotyping and MIRU-VNTR Typing.Kangjam Rekha Devi, Rinchenla Bhutia, Shovonlal Bhowmick, Kaustab Mukherjee, Jagadish Mahanta,Kanwar Narain.PLoS One. 2015; 10(12): e0145860.  Published online 2015.Dec 23. doi: 10.1371/journal.pone.0145860.PMCID: PMC4689458

Multidrug-Resistant Mycobacterium tuberculosis of the Latin American Mediterranean Lineage, Wrongly Identified as Mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), Causing Active Tuberculosis in South Brazil,Elis R. Dalla Costa, Sidra E. G. Vasconcelos, Leonardo S. Esteves, Harrison M. Gomes, Lia L. Gomes, Pedro Almeida da Silva, João Perdigão, Isabel Portugal, Miguel Viveiros, Ruth McNerney, Arnab Pain, Taane G. Clark, Nalin Rastogi, Gisela Unis, Maria Lucia R. Rossetti, Philip Noel Suffys,J Clin Microbiol. 2015 Dec; 53(12): 3805–3811.  Prepublished online.2015 Sep 23. Published online 2015 Nov 18. doi: 10.1128/JCM.02012-15.PMCID: PMC4652117

 Genetic Diversity of Mycobacterium tuberculosis Complex Isolated from Tuberculosis Patients in Bahir Dar City and Its Surroundings, Northwest Ethiopia.Anwar Nuru, Gezahegne Mamo, Adane Worku, Aschalew Admasu, Girmay Medhin, Rembert Pieper, Gobena Ameni.Biomed Res Int. 2015; 2015: 174732.Published online 2015 Sep 28. doi: 10.1155/2015/174732.PMCID: PMC4600926

 Predominance of Uganda genotype of Mycobacterium tuberculosis isolated from Ugandan patients with tuberculous lymphadenitis.Dan Wamala, Moses Okee, Edgar Kigozi, David Couvin, Nalin Rastogi, Moses Joloba, Gunilla Kallenius,BMC Res Notes. 2015; 8: 398.  Published online 2015 Sep 1. doi: 10.1186/s13104-015-1362-y.PMCID: PMC4556223

Molecular Typing of Mycobacterium Tuberculosis Complex by 24-Locus Based MIRU-VNTR Typing inConjunction with Spoligotyping to Assess Genetic Diversity of Strains Circulating in Morocco .Nada Bouklata, Philip Supply, Sanae Jaouhari, Reda Charof, Fouad Seghrouchni, Khalid Sadki, Youness El Achhab, Chakib Nejjari, Abdelkarim Filali-Maltouf, Ouafae Lahlou, Rajae El Aouad,PLoS One. 2015; 10(8): e0135695.  Published online 2015 Aug 18. doi: 10.1371/journal.pone.0135695.PMCID: PMC4540494

Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.Rodrigo Ivan Prim, Marcos André Schörner, Simone Gonçalves Senna, Christiane Lourenço Nogueira, Anna,Carolina Cançado Figueiredo, Jaquelline Germano de Oliveira, Darcita Bürger Rovaris, Maria Luiza Bazzo.Mem Inst Oswaldo Cruz. 2015 Aug; 110(5): 618–623.  doi: 10.1590/0074-02760150100.PMCID: PMC4569824

Rapid Whole-Genome Sequencing of Mycobacterium tuberculosis Isolates Directly from Clinical Samples.Amanda C. Brown, Josephine M. Bryant, Katja Einer-Jensen, Jolyon Holdstock, Darren T Houniet, Jacqueline Z. M. Chan, Daniel P. Depledge, Vladyslav Nikolayevskyy, Agnieszka Broda, Madeline J. Stone,  Mette T. Christiansen, Rachel Williams, Michael B. McAndrew, Helena Tutill, Julianne Brown, Mark Melzer, Caryn Rosmarin, Timothy D. McHugh, Robert J. Shorten, Francis Drobniewski, Graham Speight, Judith Breuer.J Clin Microbiol. 2015 Jul; 53(7): 2230–2237.  Prepublished online 2015 May 13. Published online. 2015 Jun 18. doi: 10.1128/JCM.00486-15.PMCID: PMC4473240

 

 Comparative Whole-Genome Analysis of Clinical Isolates Reveals Characteristic Architecture of Mycobacterium tuberculosis Pangenome.Vinita Periwal, Ashok Patowary, Shamsudheen Karuthedath Vellarikkal, Anju Gupta, Meghna Singh,Ashish Mittal, Shamini Jeyapaul, Rajendra Kumar Chauhan, Ajay Vir Singh, Pravin Kumar Singh, Parul,Garg, Viswa Mohan Katoch, Kiran Katoch, Devendra Singh Chauhan, Sridhar Sivasubbu, Vinod Scaria.PLoS One. 2015; 10(4): e0122979.  Published online 2015 Apr 8. doi: 10.1371/journal.pone.0122979.PMCID: PMC4390332

A First Insight on the Population Structure of Mycobacterium tuberculosis Complex as Studied by Spoligotyping and MIRU-VNTRs in Santiago, Chile.María Elvira Balcells, Patricia García, Paulina Meza, Carlos Peña, Marcela Cifuentes, David Couvin,PLoS One. 2015; 10(2): e0118007.  Published online 2015 Feb 11. doi: 10.1371/journal.pone.0118007.PMCID: PMC4324903

Multidrug-Resistant Tuberculosis Outbreak in Gaming Centers, Singapore, 2012.Cynthia B.E. Chee, Suay-Hong Gan, Rick T. Ong, Li-Hwei Sng, Christopher W. Wong, Jeffery Cutter, Min.Gong, Hui-Maan Seah, Li Yang Hsu, Suhana Solhan, Peng-Lim Ooi, Eryu Xia, Jayne T. Lim, Chwee-Kim .Koh, Soon-Kok Lim, Han-Kee Lim, Yee-Tang Wang.Emerg Infect Dis. 2015 Jan; 21(1): 179–180.  doi: 10.3201/eid2101.141159.PMCID: PMC4285258

 

2014

 

Study of Mycobacterium tuberculosis Complex Genotypic Diversity in Malaysia Reveals a Predominance of Ancestral East-African-Indian Lineage with a Malaysia-Specific SignatureFazli Ismail, David Couvin, Izzah Farakhin, Zaidah Abdul Rahman, Nalin Rastogi, Siti SuraiyaPLoS One. 2014; 9(12): e114832.  Published online 2014 Dec 11. doi: 10.1371/journal.pone.0114832.PMCID: PMC4263714

Diversity of Mycobacterium tuberculosis and drug resistance in different provinces of Papua New Guinea.Serej D Ley, Paul Harino, Kilagi Vanuga, Ruben Kamus, Robyn Carter, Christopher Coulter,  Sushil.Pandey, Julia Feldmann, Marie Ballif, Peter M Siba, Suparat Phuanukoonnon, Sebastien Gagneux,Hans-Peter Beck.BMC Microbiol. 2014; 14: 307.  Published online 2014 Dec 5. doi: 10.1186/s12866-014-0307-2.PMCID: PMC4264550

Molecular Confirmation of Bacillus Calmette Guerin Vaccine Related Adverse Events among Saudi Arabian Children.Sahal Al-Hajoj, Ziad Memish, Naila Abuljadayel, Raafat AlHakeem, Fahad AlRabiah, Bright Arghese,PLoS One. 2014; 9(11): e113472.  Published online 2014 Nov 9 doi: 10.1371/journal.pone.0113472.PMCID: PMC4237431

Tuberculosis recurrence in a high incidence setting for HIV and tuberculosis in Brazil. Gisela Unis, Andrezza Wolowski Ribeiro, Leonardo Souza Esteves, Fernanda Sá Spies, Pedro Dornelles Picon, Elis Regina Dalla Costa, Maria Lucia Rosa Rossetti,BMC Infect Dis. 2014; 14: 548.  Published online 2014 Oct 24. doi: 10.1186/s12879-014-0548-6.PMCID: PMC4215011

 Strain Classification of Mycobacterium tuberculosis Isolates in Brazil Based on Genotypes Obtained by Spoligotyping, Mycobacterial Interspersed Repetitive Unit Typing and the Presence of Large Sequence and Single Nucleotide Polymorphism.Sidra E. G. Vasconcellos, Chyntia Carolina Acosta, Lia Lima Gomes, Emilyn Costa Conceição, KarlaValéria Lima, Marcelo Ivens de Araujo, Maria de Lourdes Leite, Flávio Tannure, Paulo Cesar de Souza Caldas, Harrison M. Gomes, Adalberto Rezende Santos, Michel K. Gomgnimbou, Christophe Sola, David Couvin, Nalin Rastogi, Neio Boechat, Philip Noel SuffysPLoS One. 2014; 9(10): e107747.  Published online 2014 Oct 14. doi: 10.1371/journal.pone.0107747.PMCID: PMC4196770

Prevalence of Extensively Drug Resistant Tuberculosis among Archived Multidrug Resistant Tuberculosis Isolates in Zimbabwe.Tichaona Sagonda, Lucy Mupfumi, Rumbidzai Manzou, Beauty Makamure, Mqondisi Tshabalala, Lovemore Gwanzura, Peter Mason, Reggie Mutetwa.Tuberc Res Treat. 2014; 2014: 349141.  Published online 2014 May 20. doi: 10.1155/2014/349141.PMCID: PMC4054961

Clinico-pathological features of tuberculosis due to Mycobacterium tuberculosis Uganda genotype in patients with tuberculous lymphadenitis: a cross sectional study. Dan Wamala, Benon Asiimwe, Edgar Kigozi, Gerald Mboowa, Moses Joloba, Gunilla Kallenius.BMC Clin Pathol. 2014; 14: 14.  Published online 2014 Apr 2. doi: 10.1186/1472-6890-14-14.PMCID: PMC3974774

Strain Diversity of Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Afar Pastoral Region of Ethiopia.Mulugeta Belay, Gobena Ameni, Gunnar Bjune, David Couvin, Nalin Rastogi, Fekadu Abebe.Biomed Res Int. 2014; 2014: 238532.  Published online 2014 Mar 6. doi: 10.1155/2014/238532.PMCID: PMC3966356

 

2013

 

Molecular Epidemiology of Mycobacterium tuberculosis Complex in Singapore, 2006-2012.Leo Kang-Yang Lim, Li Hwei Sng, Wah Win, Cynthia Bin-Eng Chee, Li Yang Hsu, Estelle Mak, Arul Earnest, Marcus Eng-Hock Ong, Jeffery Cutter, Yee Tang Wang.PLoS One. 2013; 8(12): e84487.  Published online 2013 Dec 18. doi: 10.1371/journal.pone.0084487.PMCID: PMC3867517

 Global Study of IS6110 in a Successful Mycobacterium tuberculosis Strain: Clues for Deciphering Its Behavior and for Its Rapid Detection.Maria Isabel Millán-Lou, Ana Isabel López-Calleja, Cristina Colmenarejo, Maria Antonia Lezcano, Maria Asunción Vitoria, Patricia del Portillo, Isabel Otal, Carlos Martín, Sofía Samper. J Clin Microbiol. 2013 Nov; 51(11): 3631–3637.  doi: 10.1128/JCM.00970-13.PMCID: PMC3889744

Tuberculosis Transmission among Immigrants and Autochthonous Populations of the Eastern Province of Saudi Arabia.Bright Varghese, Philip Supply, Mohammed Shoukri, Caroline Allix-Beguec, Ziad Memish, Naila Abuljadayel, Raafat Al-Hakeem, Fahad AlRabiah, Sahal Al-Hajoj.PLoS One. 2013; 8(10): e77635.  Published online 2013 Oct 17. doi: 10.1371/journal.pone.0077635.PMCID: PMC379832

 Efficacy and safety of thrice weekly DOTS in tuberculosis patients with and without HIV co-infection: an observational study.Richa Vashishtha, Krishna Mohan, Bhagteshwar Singh, Satish K Devarapu, Vishnubhatla Sreenivas, Sanjay Ranjan, Deepak Gupta, Sanjeev Sinha, Surendra K Sharma.BMC Infect Dis. 2013; 13: 468.  Published online 2013 Oct 7. doi: 10.1186/1471-2334-13-468.PMCID: PMC3852441

 Extrapulmonary Tuberculosis: Mycobacterium tuberculosis Strains and Host Risk Factors in a Large Urban Setting in Brazil.Teresa Gomes, Solange Alves Vinhas, Bárbara Reis-Santos, Moisés Palaci, Renata Lyrio Peres, Paola P. Aguiar, Fabiola Karla Correa Ribeiro, Hebert Silva Marques, Valdério do Valle Dettoni, John L.

 Johnson, Lee W. Riley, Ethel Leonor Maciel PLoS One. 2013; 8(10): e74517.  Published online 2013 Oct 2. doi: 10.1371/journal.pone.0074517.PMCID: PMC3788772

Immunogenic Mycobacterium africanum Strains Associated with Ongoing Transmission in The Gambia

Florian Gehre, Martin Antonio, Jacob K. Otu, Neneh Sallah, Oumie Secka, Tutty Faal, Patrick Owiafe, Jayne S. Sutherland, Ifedayo M. Adetifa, Martin O. Ota, Beate Kampmann, Tumani Corrah, Bouke C. de Jong .Emerg Infect Dis. 2013 Oct; 19(10): 1593–1604.  doi: 10.3201/eid1910.121023.PMCID: PMC3810728

Mycobacterium tuberculosis is the causative agent of tuberculosis in the southern ecological zones of Cameroon, as shown by genetic analysis.Jean Paul Assam Assam, Véronique Penlap Beng, Fidelis Cho-Ngwa, Michel Toukam, Ane-Anyangwe Irene Ngoh, Mercy Kitavi, Inoster Nzuki, Juliette N Nyonka, Emilienne Tata, Jean Claude Tedom, Robert A Skilton, Roger Pelle, Vincent P K Titanji BMC Infect Dis. 2013; 13: 431.  Published online 2013 Sep 13. doi: 10.1186/1471-2334-13-431.PMCID: PMC3851

 Primary Drug-Resistant Tuberculosis in Hanoi, Viet Nam: Present Status and Risk Factors Nguyen Thi Le Hang, Shinji Maeda, Luu Thi Lien, Pham Huu Thuong, Nguyen Van Hung, Tran Bich Thuy,  Akiko Nanri, Tetsuya Mizoue, Nguyen Phuong Hoang, Vu Cao Cuong, Khieu Thi Thuy Ngoc, Shinsaku Sakurada, Hiroyoshi Endo, Naoto Keicho.PLoS One. 2013; 8(8): e71867.  Published online 2013 Aug 13. doi: 10.1371/journal.pone.0071867.PMCID: PMC374246

MDR TB Transmission, Singapore Cynthia B.E. Chee, Li-Yang Hsu, Li-Hwei Sng, Yee-Sin Leo, Jeffery Cutter, Yee-Tang Wang .Emerg Infect Dis. 2013 Jul; 19(7): 1151–1152.  doi: 10.3201/eid1907.120372. PMCID: PMC3713962

Prevalence of tuberculosis in pigs slaughtered at two abattoirs in Ethiopia and molecular characterization of Mycobacterium tuberculosis isolated from tuberculous-like lesions in pigs.  Sintayehu Mulugeta Arega, Franz Josef Conraths, Gobena Ameni.BMC Vet Res. 2013; 9: 97.  Published online 2013 May 6. doi: 10.1186/1746-6148-9-97.PMCID: PMC3661388

 Abattoir-based study on the epidemiology of caprine tuberculosis in Ethiopia using conventional and molecular tools. Benti Deresa, Franz J Conraths, Gobena Ameni, Acta Vet Scand. 2013; 55(1): 15.  Published online 2013 Feb 21. doi: 10.1186/1751-0147-55-15.PMCID: PMC3639213

Admixed Phylogenetic Distribution of Drug Resistant Mycobacterium tuberculosis in Saudi Arabia. Bright Varghese, Philip Supply, Caroline Allix-Béguec, Mohammed Shoukri, Ruba Al-Omari, Mais  Herbawi, Sahal Al-Hajoj.PLoS One. 2013; 8(2): e55598.  Published online 2013 Feb 1. doi: 10.1371/journal.pone.0055598

PMCID: PMC3562184

Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil

Solange Alves Vinhas, Moisés Palaci, Hebert Silva Marques, Paola Poloni Lobo de Aguiar, Fabíola Karla Ribeiro, Renata Lyrio Peres, Reynaldo Dietze, Harrison Magdinier Gomes, Philip Noel Suffys, Jonathan E. Golub, Lee W. Riley, Ethel Leonor Noia Maciel,Tuberculosis (Edinb) Author manuscript; available in PMC 2014 Mar 1. Published in final edited form as: Tuberculosis (Edinb). 2013 Mar; 93(2): 207–212. Published online 2012 Dec 8.  doi: 10.1016/j.tube.2012.09.001.PMCID: PMC3734852

Spoligotyping of multidrug-resistant Mycobacterium tuberculosis isolates in Ethiopia

B Diriba, T Berkessa, G Mamo, Y Tedla… - … International Journal of …, 2013 - ingentaconnect.com ... al.10 and as per the spoligotype kit supplier's instructions (Ocimum Biosolutions, Ijssel- stein … clinical isolates were represented by a unique (non-clustered) spoligotype pattern ... these patterns,which are already recorded in the fourth international spoligotyping database (SpolDB4 ...

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[PDF] from ajol.info

Modern lineages of Mycobacterium tuberculosis in Addis Ababa, Ethiopia: implications for the tuberculosis control programe

A Mihret, Y Bekele, M Aytenew, M Abebe, L Wassie… - African health …, 2013 - ajol.info... was carried out using the commercially available kit from Ocimum Biosolutions, India, using ... results with already existing designations in the international spoligotyping database, SpolDB4 ... 5. In this database, two or more patient isolates sharing identical spoligotype patterns are ...Related articles All 7 versions Cite

 

[HTML] from plos.org

[HTML] Admixed Phylogenetic Distribution of Drug Resistant Mycobacterium tuberculosis in Saudi Arabia

B Varghese, P Supply, C Allix-Béguec, M Shoukri… - PloS one, 2013 - dx.plos.org... grouping of the isolates with a typical Beijing spoligotype) (Figure S1). ... were identified based on isolates sharing identical MIRU-VNTR types and spoligotypes. ... loci based MIRU-VNTR typing (Genoscreen, Lille, France) and Spoligotyping (Ocimum Biosolutions, Hyderabad, India ...Related articles All 6 versions Cite More

 

[PDF] Abattoir-based study on the epidemiology of caprine tuberculosis in Ethiopia using conventional and molecular tools

B Deresa, FJ Conraths, G Ameni… - Acta Veterinaria …, 2013 - actavetscand.com ... Spoligotyping Spoligotyping was performed at ALIPB; following the procedure described earlier [28] and according to the spoligotype kit supplier's instructions (Ocimum Biosolutions Company, Iisselstein, The Netherlands). ...Related articles All 3 versions Cite

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Molecular typing of mycobacteria isolated from extrapulmonary tuberculosis patients at Debre Birhan Referral Hospital, central Ethiopia

L Garedew, A Mihret, T Abebe… - … Journal of Infectious …, 2013 - informahealthcare.com... The 34 isolates confirmed to be M. tuberculosis by deletion typing were further characterized by spoligotyping techniques, as previously described [11,12], and in accordance with the spoligotype kit supplier instructions (Ocimum Biosolutions Company, IJsselstein ...Related articles All 2 versions Cite

Endogenous reactivation followed by exogenous re-infection with drug resistant strains, a new challenge for tuberculosis control in Saudi Arabia

B Varghese, R al-Omari, C Grimshaw, S Al-Hajoj - Tuberculosis, 2013 - Elsevier... All of the available isolates from patients were subjected to spoligotyping (Ocimum Biosolutions, Hyderabad, India) and 24 loci based MIRU-VNTR typing (Genoscreen, Lille, France) by using the commercially available kits and methods described previously. ...Related articles All 4 versions Cite

 

2012

Rapid spoligotyping of Mycobacterium tuberculosis complex bacteria using a microarray system with automatic data processing and assignment

A Ruettger, J Nieter, A Skrypnyk… - Journal of Clinical …, 2012 - Am Soc Microbiol... Short-Form Paper 4 reverse line blot hybridization using the Spoligotyping Kit (Ocimum Biosolutions, Hydera- 79 bad, India) and the present DNA microarray. ... ArrayStrip spoligotyping and the assay's capability of identifying new spoligotypes and li- 109 neages. ...Import into BibTeX

 

Rapid Test for Identification of a Highly Transmissible Mycobacterium tuberculosis Beijing Strain of Sub-Saharan Origin

MI Millán-Lou, H Alonso, P Gavín… - Journal of Clinical …, 2012 - Am Soc Microbio... First, all 292 isolates were screened by spoligotyping with a commercially available kit (Ocimum Biosolutions Ltd., Hyderabad, India) by the method of Kamerbeek et al.(14). Seventy of the 292 isolates belonged to the Beijing family. ...Related articles - All 3 versions - Import into BibTeX

 

GenoType MTBDRsl performance on clinical samples with diverse genetic background

P Miotto, AM Cabibbe, P Mantegani… - European …, 2012 - Eur Respiratory Soc ... Spoligotyping. The amplified direct repeat locus of clinical isolates was investigated using the Spoligotyping kit (Ocimum Biosolutions, Hyderabad, India) following the manufacturer'sinstructions. Spoligotyping results were entered, in binary format, in the MIRU-VNTRplus web ...Related articles - All 2 versions - Import into BibTeX

 

New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Mycobacterium tuberculosis</i> strains from Saudi Arabia

B Varghese, A Hillemann, DR Wijayanti… - Infection, Genetics and …, 2012 - Elsevier... Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typingand spoligotyping. Spoligotyping was performed as previously described (Kamerbeek et al., 1997) with the commercial kit (Ocimum Biosolutions, Hyderabad, India). ...All 2 versions - Import into BibTeX

 

[HTML] DISTRIBUTION OF SPOLIGOTYPING DEFINED GENOTYPIC LINEAGES AMONG DRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS COMPLEX CLINICAL ISOLATES IN ANKARA, TURKEY

O Kisa, G Tarhan, S Gunal, A Albay, R Durmaz… - PloS one, 2012 - dx.plos.org... Spoligotyping was carried out with a commercial kit (Isogen Bioscience BV, Maarssen, The Netherlands ... tuberculosis H37Rv and M.bovis BCG P3 were used as control for spoligotyping.Spoligotypes results were converted into octal code and in the SITVIT2 proprietary database ...Related articles - Cached - All 4 versions - Import into BibTeX

 

[HTML] SPOLIGOTYPING AND DRUG RESISTANCE ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS FROM NATIONAL SURVEY IN CHINA

Y Pang, Y Zhou, B Zhao, G Liu, G Jiang, H Xia… - PloS one, 2012 - dx.plos.org... A commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as described by ... of the direct repeat region of Mycobacterium tuberculosis: implications for interpretation of spoligotyping data. ... 2004) Predominant tuberculosis spoligotypes, Delhi, India. ...Cached - Import into BibTeX

[HTML] SPOLIGOTYPING AND VARIABLE NUMBER TANDEM REPEAT ANALYSIS OF MYCOBACTERIUM BOVIS ISOLATES FROM CATTLE IN BRAZIL

PM Parreiras, GI Andrade… - Memórias do Instituto …, 2012 - SciELO Brasil... The PCR products were hybridised to a membrane (Isogen, The Netherlands) containing 43 oligonucleotides of known spacer sequences by reversed-line blot hybridisation. ... Spoligotyping - Complete spoligotypes were obtained from 61 isolates (Table I). In total, 17 ...Related articles - Cached - All 5 versions - Import into BibTeX

 

EVALUATION OF 24-LOCUS MIRU-VNTR IN EXTRAPULMONARY SPECIMENS: STUDY FROM A TERTIARY CENTRE IN MUMBAI

V Vadwai, A Shetty, P Supply… - Tuberculosis, 2012 - Elsevier... Spoligotyping, was performed using a commercial kit (Isogen, Netherlands) as per the ... 13/69,18.8%) spoligo-prototypes, compared to LAM, Haarlem or T spoligotypes represented at ... Analysis of the congruence between MIRU-VNTR and spoligotyping results combined with the …Cited by 1 - All 3 versions - Import into BibTeX

EPIDEMIOLOGICAL SIGNIFICANCE OF THE DOMESTIC BLACK PIG (SUS SCROFA) IN THE MAINTENANCE OF BOVINE TUBERCULOSIS IN SICILY

V Di Marco, P Mazzone, MT Capucchio… - Journal of Clinical …, 2012 - Am Soc Microbiol... direct repeat locus were detected by hybridization onto a spoligotyping membrane (Isogen 231 ...M. bovis isolates from both field studies were genotyped by both spoligotyping 342 ... Fourspoligotypes were identified: SB0120 (23 isolates), SB0841 (five isolates), SB0833 (two 344 ...Related articles - All 2 versions - Import into BibTeX

TRANSMISSION OF TUBERCULOSIS WITHIN FAMILY-HOUSEHOLDS

E Augustynowicz-Kopeć, T Jagielski, M Kozińska… - Journal of Infection, 2012 - Elsevier... Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen ...had different M. tuberculosis isolates, as evidenced by both spoligotyping and MIRU ... Assumingisolates revealing both identical spoligotypes and VNTR profiles are representing the ...All 2 versions - Import into BibTeX

[PDF] PREVALENCE OF PULMONARY TB AND SPOLIGOTYPE PATTERN OF MYCOBACTERIUM TUBERCULOSIS AMONG TB SUSPECTS IN A RURAL COMMUNITY IN SOUTHWEST ETHIOPIA

A Deribew, G Abebe, L Apers… - BMC Infectious …, 2012 - biomedcentral.com... Standard spoligotyping method [12] was done generally as described by Kamerbeek and colleagues using a commercially available kit (Isogen Bioscience BV ... Spoligotypes described only once (non-clustered) in this study and in the SpolDB4 were designated as ...View as HTML - Import into BibTeX

CHARACTERIZING< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES FROM KARACHI, PAKISTAN: DRUG RESISTANCE AND GENOTYPES

A Ayaz, Z Hasan, S Jafri, R Inayat, R Mangi… - International Journal of …, 2012 - Elsevier... The spoligotypes were compared with the most prevalent MTB subfamilies as identified by the World Spoligotyping Database SpolDB4.0 of the Pasteur Institute, Guadeloupe. 18. ... 3.4. Genotypicpattern of MTB isolates. Of the 1004 isolates, 987 were spoligotyped. ...All 2 versions - Import into BibTeX

IMPLICATION OF THE RD< SUP> RIO</SUP>< I> MYCOBACTERIUM TUBERCULOSIS</I> SUBLINEAGE IN MULTIDRUG RESISTANT TUBERCULOSIS IN PORTUGAL

S David, EL Duarte, CQF Leite, JN Ribeiro… - Infection, Genetics and …, 2012 - Elsevier... patient), were genotyped by spoligotyping according to the manufacturer's instructions (Isogen Bioscience BV ... Contribution of Spoligotyping to the Characterization of the population structure of ... Update on the Spoligotypes of Mycobacterium tuberculosis complex isolates from the ...Import into BibTeX

[PDF] ÇUKUROVA BÖLGESINDE AKCIĞER TÜBERKÜLOZLU HASTALARDAN İZOLE EDILEN MYCOBACTERIUM TUBERCULOSIS SUŞLARININ SPOLIGOTIPLENDIRME VE MIRU-VNTR YÖNTEMIYLE …

ÜO ZEYTİNLİ… - Mikrobiyol Bul, 2012 - mikrobiyolbul.org... Key words: Mycobacterium tuberculosis; spoligotyping; MIRU-VNTR; molecular epidemiology; Turkey. GİRİŞ ... Spoligotiplendirme Spoligotiplendirme yöntemi, üretici firmanın önerileri (Isogen Bioscience BV, Hollan- da) dikkate alınarak uygulandı. ...View as HTML - Import into BibTeX

Rapid spoligotyping of Mycobacterium tuberculosis complex bacteria using a microarray system with automatic data processing and assignment

A Ruettger, J Nieter, A Skrypnyk… - Journal of Clinical …, 2012 - Am Soc Microbiol... Short-Form Paper 4 reverse line blot hybridization using the Spoligotyping Kit (Ocimum Biosolutions, Hydera- 79 bad, India) and the present DNA microarray. ... ArrayStrip spoligotyping and the assay's capability of identifying new spoligotypes and li- 109 neages. ...Import into BibTeX

Rapid Test for Identification of a Highly Transmissible Mycobacterium tuberculosis Beijing Strain of Sub-Saharan Origin

MI Millán-Lou, H Alonso, P Gavín… - Journal of Clinical …, 2012 - Am Soc Microbiol... First, all 292 isolates were screened by spoligotyping with a commercially available kit (Ocimum Biosolutions Ltd., Hyderabad, India) by the method of Kamerbeek et al. (14). Seventy of the 292 isolates belonged to the Beijing family. ...Related articles - All 3 versions - Import into BibTeX

 

GenoType MTBDRsl performance on clinical samples with diverse genetic background

P Miotto, AM Cabibbe, P Mantegani… - European …, 2012 - Eur Respiratory Soc ... Spoligotyping. The amplified direct repeat locus of clinical isolates was investigated using the Spoligotyping kit (Ocimum Biosolutions, Hyderabad, India) following the manufacturer's instructions. Spoligotyping results were entered, in binary format, in the MIRU-VNTRplus web ...Related articles - All 2 versions - Import into BibTeX

New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Mycobacterium tuberculosis</i> strains from Saudi Arabia

B Varghese, A Hillemann, DR Wijayanti… - Infection, Genetics and …, 2012 - Elsevier... Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typing and spoligotyping. Spoligotyping was performed as previously described (Kamerbeek et al., 1997) with the commercial kit (Ocimum Biosolutions, Hyderabad, India). ...All 2 versions - Import into BibTeX

 [HTML] DISTRIBUTION OF SPOLIGOTYPING DEFINED GENOTYPIC LINEAGES AMONG DRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS COMPLEX CLINICAL ISOLATES IN ANKARA, TURKEY

[HTML] from plos.org

O Kisa, G Tarhan, S Gunal, A Albay, R Durmaz… - PloS one, 2012 - dx.plos.org... Spoligotyping was carried out with a commercial kit (Isogen Bioscience BV, Maarssen, TheNetherlands ... tuberculosis H37Rv and M.bovis BCG P3 were used as control for spoligotyping.Spoligotypes results were converted into octal code and in the SITVIT2 proprietary database ...Related articles - Cached - All 4 versions - Import into BibTeX

[HTML] SPOLIGOTYPING AND DRUG RESISTANCE ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS FROM NATIONAL SURVEY IN CHINA

Y Pang, Y Zhou, B Zhao, G Liu, G Jiang, H Xia… - PloS one, 2012 - dx.plos.org... A commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used asdescribed by ... of the direct repeat region of Mycobacterium tuberculosis: implications for interpretation of spoligotyping data. ... 2004) Predominant tuberculosis spoligotypes, Delhi, India. ...Cached - Import into BibTeX

[HTML] SPOLIGOTYPING AND VARIABLE NUMBER TANDEM REPEAT ANALYSIS OF MYCOBACTERIUM BOVIS ISOLATES FROM CATTLE IN BRAZIL

PM Parreiras, GI Andrade… - Memórias do Instituto …, 2012 - SciELO Brasil... The PCR products were hybridised to a membrane (Isogen, The Netherlands) containing 43oligonucleotides of known spacer sequences by reversed-line blot hybridisation. ... Spoligotyping -Complete spoligotypes were obtained from 61 isolates (Table I). In total, 17 ...Related articles - Cached - All 5 versions - Import into BibTeX

EVALUATION OF 24-LOCUS MIRU-VNTR IN EXTRAPULMONARY SPECIMENS: STUDY FROM A TERTIARY CENTRE IN MUMBAI

V Vadwai, A Shetty, P Supply… - Tuberculosis, 2012 - Elsevier... Spoligotyping, was performed using a commercial kit (Isogen, Netherlands) as per the ... 13/69,18.8%) spoligo-prototypes, compared to LAM, Haarlem or T spoligotypes represented at ... Analysisof the congruence between MIRU-VNTR and spoligotyping results combined with the ...Cited by 1 - All 3 versions - Import into BibTeX

EPIDEMIOLOGICAL SIGNIFICANCE OF THE DOMESTIC BLACK PIG (SUS SCROFA) IN THE MAINTENANCE OF BOVINE TUBERCULOSIS IN SICILY

V Di Marco, P Mazzone, MT Capucchio… - Journal of Clinical …, 2012 - Am Soc Microbiol... direct repeat locus were detected by hybridization onto a spoligotyping membrane (Isogen 231 ...M. bovis isolates from both field studies were genotyped by both spoligotyping 342 ... Four spoligotypes were identified: SB0120 (23 isolates), SB0841 (five isolates), SB0833 (two 344 ...Related articles - All 2 versions - Import into BibTeX

TRANSMISSION OF TUBERCULOSIS WITHIN FAMILY-HOUSEHOLDS

E Augustynowicz-Kopeć, T Jagielski, M Kozińska… - Journal of Infection, 2012 - Elsevier... Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen ...had different M. tuberculosis isolates, as evidenced by both spoligotyping and MIRU ... Assumingisolates revealing both identical spoligotypes and VNTR profiles are representing the ...All 2 versions - Import into BibTeX

[PDF] PREVALENCE OF PULMONARY TB AND SPOLIGOTYPE PATTERN OF MYCOBACTERIUM TUBERCULOSIS AMONG TB SUSPECTS IN A RURAL COMMUNITY IN SOUTHWEST ETHIOPIA

A Deribew, G Abebe, L Apers… - BMC Infectious …, 2012 - biomedcentral.com... Standard spoligotyping method [12] was done generally as described by Kamerbeek andcolleagues using a commercially available kit (Isogen Bioscience BV ... Spoligotypes described only once (non-clustered) in this study and in the SpolDB4 were designated as ...View as HTML - Import into BibTeX

CHARACTERIZING< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES FROM KARACHI, PAKISTAN: DRUG RESISTANCE AND GENOTYPES

A Ayaz, Z Hasan, S Jafri, R Inayat, R Mangi… - International Journal of …, 2012 - Elsevier... The spoligotypes were compared with the most prevalent MTB subfamilies as identified by the World Spoligotyping Database SpolDB4.0 of the Pasteur Institute, Guadeloupe. 18. ... 3.4. Genotypic pattern of MTB isolates. Of the 1004 isolates, 987 were spoligotyped. ...All 2 versions - Import into BibTeX

IMPLICATION OF THE RD< SUP> RIO</SUP>< I> MYCOBACTERIUM TUBERCULOSIS</I> SUBLINEAGE IN MULTIDRUG RESISTANT TUBERCULOSIS IN PORTUGAL

S David, EL Duarte, CQF Leite, JN Ribeiro… - Infection, Genetics and …, 2012 - Elsevier... patient), were genotyped by spoligotyping according to the manufacturer's instructions (Isogen Bioscience BV ... Contribution of Spoligotyping to the Characterization of the population structure of ... Update on the Spoligotypes of Mycobacterium tuberculosis complex isolates from the ...Import into BibTeX

[PDF] ÇUKUROVA BÖLGESINDE AKCIĞER TÜBERKÜLOZLU HASTALARDAN İZOLE EDILEN MYCOBACTERIUM TUBERCULOSIS SUŞLARININ SPOLIGOTIPLENDIRME VE MIRU-VNTR YÖNTEMIYLE …

ÜO ZEYTİNLİ… - Mikrobiyol Bul, 2012 - mikrobiyolbul.org... Key words: Mycobacterium tuberculosis; spoligotyping; MIRU-VNTR; molecular epidemiology;Turkey. GİRİŞ ... Spoligotiplendirme Spoligotiplendirme yöntemi, üretici firmanın önerileri(Isogen Bioscience BV, Hollan- da) dikkate alınarak uygulandı. ...View as HTML - Import into BibTeX

 

2011

 

[HTML] Bovine tuberculosis in a Nebraska herd of farmed elk and fallow deer: a failure of the tuberculin skin test and opportunities for serodiagnosis

W Waters, GE Stevens, MA Schoenbaum… - Veterinary medicine …, 2011 - hindawi.com... Isolates of M. bovis were identified by a combination of Ziehl-Neelsen acid-fast staining, nucleic acid probes (AccuProbe, Gen-Probe, San Diego, CA, USA), and spoligotyping (Ocimum Biosolutions Ltd., Hyderabad, India). Identification of atypical Mycobacteria spp. …Cited by 2 - Related articles - Cached - All 13 versions - Import into BibTeX

 [HTML] The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines …[HTML] from biomedcentral.com

E Abadia, J Zhang, V Ritacco… - BMC infectious …, 2011 - biomedcentral.com... of hard to interpret spoligotypes and samples representative of the work flow, so we could evaluate the benefits of a new platform regarding the classical one on these samples. Most centers had performed spoligotyping using commercial membranes (Ocimum, Hyderabad, India ...Cited by 3 - Related articles - Cached - All 26 versions - Import into BibTeX

Quality assurance for molecular epidemiology of tuberculosis methods in the mycobacterum reference laboratory

PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… - … and Quality Assurance: …, 2011 - Springer... for spoli- gotyping results, standardization and reproducibility depend on the quality of the commercial spoligotyping kit (Ocimum). ... Spoligotyping Pattern ... Both PETRA (a MIRU-VNTR lineage with central asian spoligotypes, CAS) and Beijing clusters have been observed in other ...Related articles - All 3 versions - Import into BibTeX

 

DETERMINATION OF DRUG SUSCEPTIBILITY PATTERNS AND GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM KANPUR DISTRICT; …S Purwar, S Chaudhari, VM Katoch… - Infection, Genetics and …, 2011 - Elsevier... commercially available kit 126 (Isogen Biosciences , BV, Maarsen, The Netherlands now Ocimum Biosolutions) and ... Owing to small sample size, the exact prevalence of various 272 spoligotypes and M ... Out of the isolates clustered by spoligotyping, all 280 were unique strains by ...Cited by 3 - Related articles - All 4 versions - Import into BibTeX

 

New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Mycobacterium tuberculosis</i> strains from Saudi ArabiaB Varghese, A Hillemann, DR Wijayanti… - Infection, Genetics and …, 2012 - Elsevier... Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typing and spoligotyping. Spoligotyping was performed as previously described (Kamerbeek et al., 1997) with the commercial kit (Ocimum Biosolutions, Hyderabad, India). ...All 2 versions - Import into BibTeX

 

High prevalence of subclinical tuberculosis in HIV-1-infected persons without advanced immunodeficiency: implications for TB screening

T Oni, R Burke, R Tsekela, N Bangani, R Seldon… - Thorax, 2011 - thorax.highwire.org... Lowenstein–Jensen slope cultures. The DNA was used to perform spoligotyping as previously described4 using a commercially available kit from Ocimum Biosolutions BV, The Netherlands. The hybridisation patterns obtained ...Cited by 1 - Related articles - All 12 versions - Import into BibTeX

[HTML] Pathology of camel tuberculosis and molecular characterization of its causative agents in pastoral regions of Ethiopia

G Mamo, G Bayleyegn, TS Tessema, M Legesse… - PloS one, 2011 - dx.plos.org... Spoligotyping. Spoligotyping was performed as previously described by Kamerbeek and coauthors [22] and according to the spoligotype kit supplier's instructions (Ocimum Biosolutions Company, Iisselstein, The Netherlands). ...Cited by 3 - Related articles - Cached - All 9 versions - Import into BibTeX

 

POPULATION-BASED INVESTIGATION OF FLUOROQUINOLONES RESISTANT TUBERCULOSIS IN RURAL EASTERN CHINA Y Hu, B Mathema, W Wang, B Kreiswirth, W Jiang… - Tuberculosis, 2011 - Elsevier... Spoligotyping was carried out using commercial kits from Isogen Bioscience BV (Maarssen, The Netherlands). 15 Strain family was determined by comparison of spoligotyping patterns with the SpoligDB4 database and assigned a spoligotyping international type (SIT). …Cited by 2 - Related articles - All 4 versions - Import into BibTeX

 

[HTML] THE GUINEA-BISSAU FAMILY OF MYCOBACTERIUM TUBERCULOSIS COMPLEX REVISITED [HTML] from plos.org R Groenheit, S Ghebremichael, J Svensson… - PLoS One, 2011 - dx.plos.org... All isolates that were subjected to RFLP were also spoligotyped. ... Altogether, 414 isolates were available for spoligotyping, of which 336 isolates were grouped into 42 clusters ... Out of 229 isolates, 82 different spoligotypes were obtained, of which 168 (73.4%) were clustered into ...Cited by 4 - Related articles - Cached - All 5 versions - Import into BibTeX

 

GENETIC DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS IN MBARARA, SOUTH WESTERN UGANDA [PDF] from ajol.infoJ Bazira, M Matte, BB Asiimwe… - African health sciences, 2011 - ajol.info... was performed as previously described29 using a commercially available kit (Isogen Bioscience BV ... Spoligotypes that did not match any existing pattern in the SpolDB4 database were ... tuberculosis within the Mbarara, we have used RD analysis and spoligotyping to characterize ...Related articles - All 8 versions - Import into BibTeX

FLUOROQUINOLONE RESISTANCE AMONG MYCOBACTERIUM TUBERCULOSIS STRAINS FROM KARACHI, PAKISTAN: DATA FROM COMMUNITY-BASED FIELD CLINICS TML] from nih.gov  Rafiq, K Jabeen, R Hasan, S Jafri… - Antimicrobial agents …, 2011 - Am Soc Microbiol... was performed on all FQ-resistant strains using a commercially available kit provided by Isogen Life Science BV ... The spoligotypes were compared with the most prevalent M. tuberculosis subfamilies, as identified by the world spoligotyping database, SpolDB4.0, of ...Related articles - All 6 versions - Import into BibTeX

[HTML] CHARACTERISTICS OF PNCA MUTATIONS IN MULTIDRUG-RESISTANT TUBERCULOSIS IN TAIWAN [HTML] from biomedcentral.com YC Chiu, SF Huang, KW Yu, YC Lee… - BMC infectious …, 2011 - biomedcentral.com... Genotyping. All clinical isolates were genotyped by a commercial spoligotyping kit (Isogen Bioscience BV, Maarssen, Netherlands). The "Beijing strain" was defined as deletion from spacer 1 to spacer 34 in the direct repeat region and the presence of (at least 3) spacers 35-43. ...Related articles - Cached - All 11 versions - Import into BibTeX

[HTML] INITIAL PRESENTATIONS PREDICT MORTALITY IN PULMONARY TUBERCULOSIS PATIENTS-A PROSPECTIVE OBSERVATIONAL STUDY [HTML] from plos.org JY Feng, WJ Su, YC Chiu, SF Huang, YY Lin… - PloS one, 2011 - dx.plos.org... All clinical isolates were genotyped using a commercial spoligotyping kit (Isogen Bioscience BV, Maarssen, Netherlands). The “Beijing strain” was defined as a deletion from spacer 1 to spacer 34 in the direct repeat region and the presence of (at least 3) spacers in 35–43. ...Related articles - Cached - All 4 versions - Import into BibTeX

[HTML] MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS, BUENOS AIRES, ARGENTINA [HTML] from nih.govX Gonzalo, M Ambroggi, E Cordova… - Emerging Infectious …, 2011 - ncbi.nlm.nih.gov... DNA was extracted from cultures at this UK laboratory; spoligotyping was performed according to the manufacturer's instructions (Isogen Life Science, IJsselstein, the Netherlands); and data were analyzed with SPOTCLUST (http://cgi2.cs.rpi.edu/~bennek/SPOTCLUST.html). ...Related articles - All 4 versions - Import into BibTeX

CHARACTERISTICS OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS IN TAIWAN: A POPULATION-BASED STUDY CW Chang, MH Wu, PC Chuang… - Infection, Genetics and Evolution, 2011 - Elsevier... 12 13 Spoligotyping 14 A commercially available kit (Isogen Bioscience BV, Maarssen, The 15 Netherlands) was used as ... Even through spoligotyping results 3 revealed the genetic diversities of MDR M. tuberculosis isolates in Taiwan, 4 identical spoligotypes could not ...Cited by 2 - Related articles - All 2 versions - Import into BibTeX

MOLECULAR PATTERN OF MYCOBACTERIUM BOVIS ISOLATES AND ITS RELATIONSHIP WITH RISK FACTORS ASSOCIATED WITH THE PRESENCE OF BOVINE …[PDF] from uaemex.mx IF Padilla-Campos, JC Segura-Correa… - Tropical and …, 2011 - redalyc.uaemex.mx... Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors. ... Spoligotyping was carried out following the protocol described by Kamerbeek et al ... sequences from M. tuberculosis and six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The ...Related articles - All 2 versions - Import into BibTeX

[HTML] FINE-TUNING THE SPACE, TIME, AND HOST DISTRIBUTION OF MYCOBACTERIA IN WILDLIFE[HTML] from biomedcentral.com C Gortazar, MJ Torres, P Acevedo, J Aznar… - BMC …, 2011 - biomedcentral.com... Typing. The M. bovis isolates were further characterized by spoligotyping [40]. The amplified product was detected by hybridization of the biotin-labelled PCR product onto spoligotyping membrane (Isogen Bioscience BV, Maarssen, The Netherlands). ...Cited by 6 - Related articles - Cached - All 19 versions - Import into BibTeX

MOLECULAR PATTERN OF MYCOBACTERIUM BOVIS ISOLATES AND ITS RELATIONSHIP WITH RISK FACTORS ASSOCIATED WITH THE PRESENCE OF …[PDF] from uady.mx IF Padilla, JC Segura, JC Rodriguez… - Tropical and …, 2011 - ccba.uady.mx... Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors. ... Spoligotyping was carried out following the protocol described by Kamerbeek et al ... sequences from M. tuberculosis and six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The ...Related articles - All 2 versions - Import into BibTeX

EPIDEMIOLOGICAL TRENDS AND CLINICAL COMPARISONS OF< I> MYCOBACTERIUM</I>< I> TUBERCULOSIS</I> LINEAGES IN THAI TB MENINGITISK Faksri, F Drobniewski, V Nikolayevskyy, T Brown… - Tuberculosis, 2011 - Elsevier... isolated from CSF samples collected from 1996 to 2004 with IS6110 RFLPs (120 RFLP patterns were available) and spoligotyping results (141 spoligotypes were available ... 27 Spoligotypingwas performed according to the manufacturer's instructions (Isogen Bioscience BV ...Cited by 1 - Related articles - All 3 versions - Import into BibTeX

A NOVEL METHOD FOR THE RAPID AND PROSPECTIVE IDENTIFICATION OF BEIJING MYCOBACTERIUM TUBERCULOSIS STRAINS BY HIGH‐RESOLUTION MELTING ANALYSISM Alonso, Y Navarro, F Barletta… - Clinical …, 2011 - Wiley Online Library... Thirty-two MTB strains (18 representatives of the Beijing genotype and 14 representatives of the non-Beijing genotype, according to their spoligotypes) were grown on ... Spoligotyping was performed in accordance with the manufacturer's instructions (Isogen, De Meern, the ...Cited by 3 - Related articles - All 3 versions - Import into BibTeX

QUALITY ASSURANCE FOR MOLECULAR EPIDEMIOLOGY OF TUBERCULOSIS METHODS IN THE MYCOBACTERIUM REFERENCE LABORATORY PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… - … and Quality Assurance: …, 2011 - Springer... 43 spacer spoligotyping was performed according to the manufacturer's instructions (Isogen/Ocimun) as described previously. ... 27 Spoligotyping Pattern ... Both PETRA (a MIRU-VNTR lineage with central asian spoligotypes, CAS) and Beijing clusters have been observed in other ...Related articles - All 3 versions - Import into BibTeX

EPIDEMIOLOGICAL ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED IN LODZ, POLANDM Krawczyk, A Brzostek, A Gorna… - … of Tuberculosis and …, 2011 - ingentaconnect.com... of van Embden et al.16 Spoligotyping was performed as described by Kamerbeek et al., using the Isogen Life Science kit ... Spoligotyping analysis showed that 184 (78.6%) of the strains were grouped in clusters and 50 (21.4%) strains possessed unique spoligotypes. ...

 

ONE YEAR NATIONWIDE EVALUATION OF 24-LOCUS MIRU-VNTR GENOTYPING ON SLOVENIAN< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES U Bidovec-Stojkovic, M Zolnir-Dovc… - Respiratory Medicine, 2011 - Elsevier... 4,9,20 Spoligotyping was performed using a commercially available kit (Isogen Bioscience B identically defined by IS6110 RFLP and 24-locus MIRU-VNTR typing combined with spoligotyping. ...jointly defined by identical 24-locus MIRU-VNTR types and spoligotypes were only ...

 

MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX (MTBC) ISOLATED FROM CATTLE IN NORTHEAST AND NORTHWEST CHINA Y Du, Y Qi, L Yu, J Lin, S Liu, H Ni… - Research in veterinary …, 2011 - Elsevier... 2.5. Spoligotyping. Spoligotyping kits and Mini-blotter MN45 were obtained from Isogen Life Science Co. (Utrecht, the Netherlands). The Streptavidin-ß-peroxidase conjugate was produced by Roche Applied Science Co. (Penzberg, Germany). ...Cited by 3 - Related articles - All 3 versions - Import into BibTeX

[HTML] TRANSMISSION PATTERN OF DRUG-RESISTANT TUBERCULOSIS AND ITS IMPLICATION FOR TUBERCULOSIS CONTROL IN EASTERN RURAL CHINA [HTML] from plos.org Y Hu, B Mathema, W Jiang, B Kreiswirth, W Wang… - PloS one, 2011 - dx.plos.org... Hybridized DNA was detected using an enhanced chemiluminescene kit from Isogen Bioscience BV ... b Probaiblity that the Spoligotyping pattern belongs to the clades. ... N, Bennett KP (2006) Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. ...Related articles - Cached - All 7 versions - Import into BibTeX

SUBLINEAGES OF BEIJING STRAIN OF MYCOBACTERIUM TUBERCULOSIS IN SRI LANKA US Rajapaksa… - Indian Journal of Microbiology, 2011 - Springer... Spoligotyping was carried out according to manufacturer's guidelines using a commercially available kit (Isogen, Maarsen MV, The Netherland). Fourteen iso-lates that confirmed to be Beijing strain were further characterised. ...Related articles - All 2 versions - Import into BibTeX

DETERMINATION OF DRUG SUSCEPTIBILITY PATTERNS AND GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM KANPUR DISTRICT; …S Purwar, S Chaudhari, VM Katoch… - Infection, Genetics and …, 2011 - Elsevier... 43 spacers was done with the help of a commercially available kit 126 (Isogen Biosciences ,BV ... Owing to small sample size, the exact prevalence of various 272 spoligotypes and M.tuberculosis ... Out of the isolates clustered by spoligotyping, all 280 were unique strains by IS6110 ...Cited by 3 - Related articles - All 4 versions - Import into BibTeX

MOLECULAR EPIDEMIOLOGY OF HUMAN AND ANIMAL TUBERCULOSIS IN IBADAN, SOUTHWESTERN NIGERIA AO Jenkins, SIB Cadmus, EH Venter… - Veterinary …, 2011 - Elsevier... Spoligotyping167 Spoligotyping confirmed the species identification of 19 M. tuberculosis and 13 M ... None of these CAM 173 variant spoligotypes were found in the spolDB4 database and were ...All M. tuberculosis strains 175 from PTB patients that were spoligotyped had identical ...Cited by 1 - Related articles - All 5 versions - Import into BibTeX

 

GENETIC DIVERSITY OF THE MYCOBACTERIUM TUBERCULOSIS BEIJING FAMILY BASED ON IS6110, SNP, LSP AND VNTR PROFILES FROM THAILANDK Faksri, F Drobniewski, V Nikolayevskyy… - Infection, Genetics and …, 2011 - Elsevier... Eighty MTB Beijing isolates from CSF samples with IS6110 RFLPs (69 RFLP patterns wereavailable) and spoligotyping results (all 80 spoligotypes were available ... Spoligotyping was performed according to the manufacturer s instructions (Isogen Bioscience BV ...Cited by 2 - Related articles - All 3 versions - Import into BibTeX

[PDF] 骨结核临床分离菌株基因分型的特点 [PDF] from clinicmed.net李文胜， 刘毅， 孙勇， 田苗， 刘忠泉… - 中华临床医师杂志 ( …, 2011 - clinicmed.net... TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自TaＫaRa 公司。凝胶成像仪购自FOTO DＹNE 公司。 Spoligotyping 试 剂盒以及Miniblotter MN４５为荷兰 Isogen Biosｃienｃe 公司产品。 链霉亲和素为Sigam 公司产品。 化学发光 ...Related articles - View as HTML - All 3 versions - Import into BibTeX

MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS IN THE UNITED ARAB EMIRATES [PDF] from qmul.ac.uk A Saedi… - 2011 - qmro.qmul.ac.uk ... 130 3.2.11 Identification of the UAE isolates by spoligotyping..... 132 3.2.12 Comparison betweenMIRU-typed and spoligotyped families in association with katG463/gyrA95 groupings ..... … Related articles - All 2 versions - Import into BibTeX 

 

[HTML] MYCOBACTERIUM BOVIS IN SWINE: SPOLIGOTYPING OF ISOLATES FROM ARGENTINA [HTML] from hindawi.comS Barandiaran, EV Moras, A Adri& - Veterinary Medicine …, 2011 - hindawi.com... Spoligotyping [14] was carried out by using the spoligotyping kit (Isogen Biosolutions BV, Ocimun ...Guerin (BCG) (ATCC 27289) were included as reference strains in each spoligotyping experiment ...isolates were defined as two or more M. bovis strains with identical spoligotypes. ...Cited by 1 - Related articles - Cached - All 11 versions - Import into BibTeX

[HTML] SPOLIGOTYPING OF CLINICAL MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM THE STATE OF MINAS GERAIS, BRAZIL [HTML] from scielo.brSS Miranda, WS Carvalho, PN Suffys… - Memórias do Instituto …, 2011 - SciELO Brasil... was resuspended in the same TE buffer, stored at -20ºC and subjected to spoligotyping using a previously published protocol (Kamerbeek et al. 1997) and a commercially available kit (Isogen Life Science BV, Utrecht, The Netherlands). The hybridised spoligotypes in binary ...Related articles - Cached - All 7 versions - Import into BibTeX

EVALUATION OF SPOLIGOTYPING AND MIRU-VNTR FOR MYCOBACTERIUM BOVIS IN XINJIANG, CHINAZ Sun, R Cao, M Tian, X Zhang, X Zhang… - Research in Veterinary …, 2011 - Elsevier... Additionally, two new spoligotypes (SB1903 and SB1904) and special repeat numbers of 3 loci (ETR ... Spoligotyping was firstly analyzed and interpreted in octal codes (Dale et al., 2001) following the instruction (http://www.mbovis.org) with a commercial kit (Isogen Bioscience BV ...Cited by 2 - Related articles - All 2 versions - Import into BibTeX

[HTML] MYCOBACTERIUM TUBERCULOSIS SPOLIGOTYPES AND DRUG SUSCEPTIBILITY PATTERN OF ISOLATES FROM TUBERCULOSIS PATIENTS IN SOUTH-WESTERN UGANDA [HTML] from biomedcentral.comJ Bazira, BB Asiimwe, ML Joloba… - BMC infectious …, 2011 - biomedcentral.com... Spoligotyping. Standard spoligotyping was performed as previously described [29] using a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands). ... Spoligotyping.All the isolates had spoligotypes characteristic of M. tuberculosis. ...Cited by 1 - Related articles - Cached - All 12 versions - Import into BibTeX

A FIRST INSIGHT ON THE POPULATION STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS COMPLEX AS STUDIED BYSPOLIGOTYPING AND MIRU-VNTRS IN BOGOTÁ, COLOMBIAI Cerezo, Y Jiménez, J Hernandez, T Zozio… - Infection, Genetics and …, 2011 - Elsevier... Kamerbeek et al., 1997) according to the manufacturer s instructions (Isogen Bioscience, the ...Spoligotype International Type (SIT) and MIRU International Type (MIT) designate spoligotypes and MIRU ... was used to build minimum spanning trees based on spoligotyping and MIRU ...All 2 versions - Import into BibTeX

[PDF] PRELIMINARY MAPPING STUDY OF MYCOBACTERIUM TUBERCULOSIS' COMPLEX DNA BYSPOLIGOTYPING ON ISOLATED SPUTUM OF TUBERCULOSIS PATIENT …[PDF] from digitaljournals.org V Lisdawati, I Parwati… - Bulletin of Health …, 2011 - indonesia.digitaljournals.org... PCR, denaturasi menggunakan thermocycler selama 10 menit pada suhu 99,9°C. Kemudian pada mini blotter MN45 yang telah diletakkan membran spoligotyping kit (IM9702, Isogen Biosolution, BV) berlabel 43 DNA spacer Mtb complex segera hibridisasikan DNA hasil PCR. ...Related articles - View as HTML - Import into BibTeX

[PDF] INFECTION OF EURASIAN BADGERS (MELES MELES) WITH MYCOBACTERIUM BOVIS[PDF] from uclm.esA Balseiro, O Rodríguez, P González-Quirós… - prevalence, 2011 - uclm.es... al., 2009). 125 MTC isolates were characterised hybridisation of biotin-labelled PCR products 126 onto a spoligotyping membrane (Isogen Bioscience BV), followed by spoligotyping 127 (Kamerbeek et al., 1997). Results were ...Cited by 2 - Related articles - View as HTML - All 2 versions - Import into BibTeX

CLINICAL AND MICROBIOLOGICAL CHARACTERISTICS OF URINE CULTURE-CONFIRMED GENITOURINARY TUBERCULOSIS AT MEDICAL CENTERS IN TAIWAN FROM 1995 TO 2007HL Hsu, CC Lai, MC Yu, FL Yu, JC Lee… - European journal of …, 2011 - Springer... commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as described by the manufacturer. The fourth international spoligotyping database (SpolDB4) database was used to assign genotype families to the spoligotypes obtained [14 ...Related articles - All 5 versions - Import into BibTeX

MOLECULAR TYPING OF MYCOBACTERIUM TUBERCULOSIS CIRCULATED IN MOSCOW, RUSSIAN FEDERATIONMV Afanas' ev, LN Ikryannikova, EN Il'ina… - European Journal of …, 2011 - Springer... spacers probes from the spoligotyping kit from Isogen Lifescience (cat. no. IM9701). 182 Eur J Clin Microbiol Infect Dis (2011) 30:181–191 Page 3. Spoligotypes in binary format were entered in an Excel spreadsheet and compared with the spoligotyping database SpolDB4 [13]. ...Cited by 7 - Related articles - All 4 versions - Import into BibTeX

ROLE OF 2-MONTH SPUTUM SMEARS IN PREDICTING CULTURE CONVERSION IN PULMONARY TUBERCULOSIS[HTML] from ersj.org.uk WJ Su, JY Feng, YC Chiu… - European Respiratory …, 2011 - Eur Respiratory Soc... All clinical isolates were genotyped by commercial spoligotyping kit (Isogen Bioscience BV, Maarssen, the Netherlands). The “Beijing strain” was defined as deletion from spacer 1 to spacer 34 in the direct repeat region and the presence of (at least three of) spacers 35–43. ...Cited by 5 - Related articles - All 6 versions - Import into BibTeX

[PDF] A TWO-STEP STRATEGY FOR MOLECULAR TYPING OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATES FROM POLAND. [PDF] from microbiology.pl T JAGIELSKI, EWAA KOPEĆ… - Polish journal of …, 2011 - pjm.microbiology.pl... Spoligotyping was perfor- med with a commercially available kit (Isogen Bio- science BV ... e isolates whose spoligotype patterns were already recorded in the database, were assigned“shared types”, whereas those of spoligotypes identified for the first time, were ...View as HTML - All 3 versions - Import into BibTeX

MOLECULAR TECHNOLOGIES USED IN DETECTING OF SENSITIVE AND ISONIAZID-RESISTANT MYCOBACTERIUM TUBERCULOSIS OY Limanskaya… - Cytology and Genetics, 2011 - Springer... The PCR was carried out by a dry kit of reagents for DNA amplification (Isogen, Russian Federation) in 20–50 μl of reaction mixture containing 4 mM NaCl, 50 mM KCl, 12 mM Tris HCl (pH 8.0), 2.5 mM MgCl2, 200 μM dNTPs, 0.1–0.5 μM of each primer, and 1 unit of Taq DNA ...Related articles - All 4 versions - Import into BibTeX

STREPTOMYCIN RESISTANCE AND LINEAGE-SPECIFIC POLYMORPHISMS IN MYCOBACTERIUM TUBERCULOSIS GIDB GENE HTML] from nih.govFS Spies, AW Ribeiro, DF Ramos… - Journal of clinical …, 2011 - Am Soc Microbiol... Genotyping.Spoligotyping was performed using a commercial kit (Isogen Biosciences BV, Netherlands ... it was classified as LAM3 and S/convergent sublineage by spoligotyping but as non … Spoligotypes evolve through successive loss of spacer DNA sequences (16), and since ...Cited by 2 - Related articles - All 7 versions - Import into BibTeX

GENOTYPING AND DRUG RESISTANCE PATTERNS OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN FIVE PROVINCES OF CHINA YL Guo, Y Liu, SM Wang, CY Li… - … of Tuberculosis and …, 2011 - ingentaconnect.com... Of the (Isogen Bioscience BV, De Meern, The Netherlands). ... 1–1800 = shared spoligotypes. Table 3 Results of MIRU typing and comparison of the three typing methods Isolate MIRU locus number IS6110-RFLP Cluster 2 4 10 16 20 23 24 26 27 31 39 40 Spoligotyping MIRU ...Cited by 3 - Related articles - All 3 versions - Import into BibTeX

ASSOCIATION OF< I> MYCOBACTERIUM TUBERCULOSIS</I> GENOTYPES AND CLINICAL AND EPIDEMIOLOGICAL FEATURES-A MULTI-CENTER STUDY IN TAIWAN SF Huang, WJ Su, HY Dou, JY Feng… - Infection, Genetics and …, 2011 - Elsevier... Spoligotyping was performed according to the method reported by Kamerbeek et al. (Kamerbeek et al., 1997) using a commercial kit (Isogen Biosolutions, Netherlands). The spoligotypes were identified according to the database SpoDB4.0 (Brudey et al., 2006), and the SIT ...Related articles - All 5 versions - Import into BibTeX

THE GENOTYPE CHARACTERISTIC OF CLINICAL ISOLATED STRAINS GETTING FROM BONE TUBERCULOSIS[PDF] from clinicmed.netLI Wen-sheng, LIU Yi, SUN Yong… - Chinese Journal of …, 2011 - global.clinicmed.net... TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自 TaＫaRa 公司。 凝胶成像仪购自 FOTO DＹNE 公司。 Spoligotyping 试 剂盒以及 Miniblotter MN４５为荷兰 Isogen Biosｃienｃe 公司产品。 链霉亲和素为 Sigam 公司产品。 化学发光 ...Related articles - Import into BibTeX

MULTIPLE STRAINS OF< I> MYCOBACTERIUM BOVIS</I> REVEALED BY MOLECULAR TYPING IN A HERD OF CATTLE EES Figueiredo, DF Ramos, L Medeiros… - The Veterinary …, 2011 - Elsevier... to the spoligo-membrane was performed according to the manufacturer's instructions (Isogen Life Sciences). ... The strains clustered by spoligotyping were analysed by VNTR-PCR to confirm clonal relationships ... Four spoligotypes were identified among the 12 M. bovis isolates. ...Related articles - All 2 versions - Import into BibTeX

DETERMINAÇÃO DO CUSTO BIOLÓGICO EM ISOLADOS DE MYCOBACTERIUM TUBERCULOSIS COM MUTAÇÕES NOS GENES RPSL, RRS, GIDB, RPOB E KATG [PDF] from ufrgs.br FS Spies - 2011 - lume.ufrgs.br... restriction fragment lenght polymorphism) da sequência de inserção IS6110, spoligotyping e unidade de repetição de micobactéria- repetição em tandem de número variável (MIRU- ...Spoligotyping é uma técnica de genotipagem que explora a variabilidade da região ...Related articles - Import into BibTeX

A MAJOR SUBGROUP OF BEIJING FAMILY MYCOBACTERIUM TUBERCULOSIS IS ASSOCIATED WITH MULTIDRUG RESISTANCE AND INCREASED TRANSMISSIBILITY Y Hu, X Ma, EA Graviss, W Wang… - Epidemiology and …, 2011 - Cambridge Univ Press... Spoligotyping was performed by using a com- mercial kit from Isogen Bioscience BV (Maarssen ...0.04), and resistance to RIF and SM (P=0.04) (Table 1). Spoligotyping identified 243 ... Thirty-three(9.4%) isolates had unique/novel spoligotypes, of which similarity analysis found that ...Cited by 6 - Related articles - All 4 versions - Import into BibTeX

[HTML] MODERN AND ANCESTRAL GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS FROM ANDHRA PRADESH, INDIA [HTML] from plos.org SK Thomas, CC Iravatham, BH Moni, A Kumar… - PloS one, 2011 - dx.plos.org ... the help of commercially available line probe arrays (Isogen Biosciences BV ... tuberculosis complexgenetic diversity: mining the fourth international spoligotyping database (SpolDB4 ... KP (2006)Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. ...Related articles - Cached - All 5 versions - Import into BibTeX

[HTML] TUBERCULOSIS IN SUDAN: A STUDY OF MYCOBACTERIUM TUBERCULOSIS STRAIN GENOTYPE AND SUSCEPTIBILITY TO ANTI-TUBERCULOSIS DRUGS [HTML] from biomedcentral.com GSS Eldin, I Fadl-Elmula, MS Ali… - BMC infectious …, 2011 - biomedcentral.com... 21] using a commercial kit to test for 43 individual spacers (Isogen, IJsselstein, The ... Spoligotyping provided a relatively simple and rapid means of describing the population of ... improved discrimination of strains sharing the CAS1_Dehli and Beijing spoligotypes when using MIRU ...Related articles - Cached - All 11 versions - Import into BibTeX

[PDF] MOLECULAR CHARACTERISATION OF MYCOBACTERIUM BOVIS ISOLATED FROM AFRICAN BUFFALOES (SYNCERUS CAFFER) IN HLUHLUWE-IMFOLOZI PARK IN KWAZULU-NATAL, SOUTH AFRICA [PDF] from scielo.org.za TM Hlokwe, AO Jenkins, EM Streicher… - … Journal of Veterinary …, 2011 - scielo.org.za... One of the observed spoligotypes (SB0130) was dominant and represented 75% of isolates ...Spoligotyping Spoligotyping was done according to a standardised international method(Kamerbeek et al. 1997) using a commercially available kit (Isogen Biosciences BV, Maarsen). ...Related articles - View as HTML - All 10 versions - Import into BibTeX

GENOTYPING DID NOT EVIDENCE ANY CONTRIBUTION OF MYCOBACTERIUM BOVIS TO HUMAN TUBERCULOSIS IN BRAZIL [PDF] from redetb.org A Rocha, AR Elias, LF Sobral, DF Soares… - Tuberculosis, 2011 - Elsevier... using PCR and hybridization conditions on a commercially available membrane (Isogen Biosciene BV ... All isolates that yielded spoligotypes that were recognized as not previously described patterns were submitted to at least one additional spoligotyping assay and ...Related articles - All 6 versions - Import into BibTeX

MOLECULAR AND EPIDEMIOLOGICAL CHARACTERIZATION OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS ISOLATES IN JOHANNESBURG, SOUTH AFRICA [PDF] from wits.ac.zaCK Mlambo - 2011 - wiredspace.wits.ac.za ... 61 Table 3.6 Description of the orphan MIRU patterns (n=45) from this study and corresponding spoligotyping-based information (octal code, SIT, and clade)…………64 Table 3.7 MIRU profiles of the four newly created spoligotypes (10 ...Related articles - Import into BibTeX

USE OF SPOLIGOTYPE ANALYSIS TO DETECT LABORATORY CROSS-CONTAMINATIONB Nivin, J Driscoll, T Glaser, P Bifani… - Infection control and …, 2000 - JSTOR ... The spoligotyping analysis was performed using a commercially available kit (Isogen Bioscience BV ... In several cases of identical spoligotypes, the Wadsworth Center indicated that the ...Spoligotyping is an effective, relatively inexpensive (approximately 10% the cost of RFLP ...Cited by 12 - Related articles - BL Direct - All 6 versions - Import into BibTeX

CLINICAL AND GENOTYPIC CHARACTERISTICS OF CHILDHOOD TUBERCULOSIS IN CHONGQING, CHINA L Xing, R Liu, Q Li, Z Peng… - European Journal of Clinical …, 2011 - Springer... Of 105 isolates spoligotyped, the major spoligotypes found were ... Spoligotyping was performed according to the internation- ally standardized protocol [5]. A commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as described ...Related articles - All 2 versions - Import into BibTeX

TUBERCULOSIS DUE TO MYCOBACTERIUM BOVIS AND MYCOBACTERIUM CAPRAE IN SHEEPM Muņoz Mendoza, L Juan, S Menéndez… - The Veterinary …, 2011 - Elsevier

... by hybridisation of the biotin-labelled PCR product onto spoligotyping membrane (Isogen Bioscience BV). ... Isolates were characterised by spoligotyping as M. caprae SB0157 (flock A) and M. bovis ... close contact with cattle or goats infected by the same spoligotypes (Table 1 ...Cited by 3 - Related articles - All 2 versions - Import into BibTeX

[HTML] MYCOBACTERIUM BOVIS INFECTION IN GOATS FROM THE NORTHEAST REGION OF BRAZIL [HTML] from scielo.br SSS Higino, SR Pinheiro, GO Souza… - Brazilian Journal of …, 2011 - SciELO Brasil... Isolate was characterised by spoligotyping as described (7). The amplified product was detected by hybridization of the biotin-labelled PCR product onto spoligotyping membrane (Isogen Bioscience BV, Maarssen, The Netherlands). ...

EPIDEMIOLOGICAL STUDIES OF BEIJING STRAINS OF MYCOBACTERIUM TUBERCULOSIS FROM TAIPEI AND OTHER ASIAN CITIES BASED ON MIRU PROFILES JUNREN SUN, HYUN DOU, SYI LEE… - Apmis, 2011 - Wiley Online Library... on isolates from areas where the Beijing strain is prevalent as most isolates in this family share the same spoligotypes (1, 5 ... Spoligotyping ... to the manufacturers' protocol, to a set of 43 oligonucle-otides of DR spacers on a membrane using the Isogen Bioscience spoligotyping kit ...

 

 

 

 

Year 2012

 

 Diversity of Mycobacterium tuberculosis Isolates from New Pulmonary Tuberculosis Cases in Addis Ababa, Ethiopia.Adane Mihret, Yonas Bekele, Andre G. Loxton, Annemie M. Jordan, Lawrence Yamuah, Abraham Aseffa, Rawleigh Howe, Gerhard Walzl,Tuberc Res Treat. 2012; 2012: 892079.  Published online 2012 Nov 22. doi: 10.1155/2012/892079.PMCID: PMC3513727

 

 Plasma Level of IL-4 Differs in Patients Infected with Different Modern Lineages of M. tuberculosis.Adane Mihret, Yonas Bekele, Andre G. Loxton, Abraham Aseffa, Rawleigh Howe, Gerhard Walzl,J Trop Med. 2012; 2012: 518564.  Published online 2012 Sep 25. doi: 10.1155/2012/518564.PMCID: PMC3463165

 

 Modern lineages of Mycobacterium tuberculosis in Addis Ababa, Ethiopia: implications for the tuberculosis control programe.A Mihret, Y Bekele, M Aytenew, Y Assefa, M Abebe, L Wassie, GA Loxton, L Yamuah, A Aseffa, G Walzl, R Howe.Afr Health Sci. 2012 Sep; 12(3): 339–344.  PMCID: PMC3557690

 

 Rapid Spoligotyping of Mycobacterium tuberculosis Complex Bacteria by Use of a Microarray System with Automatic Data Processing and Assignment.Anke Ruettger, Johanna Nieter, Artem Skrypnyk, Ines Engelmann, Albrecht Ziegler, Irmgard Moser, Stefan Monecke, Ralf Ehricht, Konrad Sachse

J Clin Microbiol. 2012 Jul; 50(7): 2492–2495.  doi: 10.1128/JCM.00442-12

  Whole cell & culture filtrate proteins from prevalent genotypes of Mycobacterium tuberculosis provoke better antibody & T cell response than laboratory strain H37Rv.Gavish Kumar, Hari Shankar, Mamta Chahar, Pragya Sharma, Virendra Singh Yadav, Devendra Singh.Chauhan, Vishwa Mohan Katoch, Beenu Joshi

Indian J Med Res. 2012 May; 135(5): 745–755. PMCID: PMC3401709

 

 Rapid Test for Identification of a Highly Transmissible Mycobacterium tuberculosis Beijing Strain of Sub-Saharan Origin.María Isabel Millán-Lou, Henar Alonso, Patricia Gavín, Melissa Hernández-Febles, María Isolina

Campos-Herrero, Rodolfo Copado, Fernando Cañas, Kristin Kremer, José Antonio Caminero, Carlos  Martín, Sofía Samper.J Clin Microbiol. 2012 Feb; 50(2): 516–518.  doi: 10.1128/JCM.06314-11.PMCID: PMC3264144

 

2011

High prevalence of subclinical tuberculosis in HIV-1-infected persons without advanced  immunodeficiency: implications for TB screening.Tolu Oni, Rachael Burke, Relebohile Tsekela, Nonzwakazi Bangani, Ronnett Seldon, Hannah P Gideon, Kathryn Wood, Katalin A Wilkinson, Tom H M Ottenhoff, Robert J Wilkinson.Thorax. 2011 Aug; 66(8): 669–673.  Published online 2011 May 31. doi: 10.1136/thx.2011.160168

PMCID: PMC3142344

 

The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines for Quality Assurance when working on membranes

Edgar Abadia, Jian Zhang, Viviana Ritacco, Kristin Kremer, Raymond Ruimy, Leen Rigouts, Harrison Magdinier Gomes, Atiná Ribeiro Elias, Maryse Fauville-Dufaux, Karolien Stoffels, Voahangy Rasolofo-Razanamparany, Darío Garcia de Viedma, Marta Herranz, Sahal Al-Hajoj, Nalin Rastogi, Carlo Garzelli, Enrico Tortoli, Philip N Suffys, Dick van Soolingen, Guislaine Refrégier, Christophe Sola.BMC Infect Dis. 2011; 11: 110.  Published online 2011 Apr 28. doi: 10.1186/1471-2334-11-110.PMCID: PMC3107175

 

Bovine Tuberculosis in a Nebraska Herd of Farmed Elk and Fallow Deer: A Failure of the Tuberculin Skin Test and Opportunities for Serodiagnosis.W. Ray Waters, Gary E. Stevens, Mark A. Schoenbaum, Kathy A. Orloski, Suelee Robbe-Austerman, N. Beth Harris, S. Mark Hall, Bruce V. Thomsen, Arach J. Wilson, Roger E. Brannian, Jeffrey T. Nelson, Shawn Schafer, Javan Esfandiari, Meghan Dutton, Rena Greenwald, Konstantin P. Lyashchenko.Vet Med Int. 2011; 2011: 953985.  Published online 2011 Apr 14. doi: 10.4061/2011/953985

PMCID: PMC3087612

 

Pathology of Camel Tuberculosis and Molecular Characterization of Its Causative Agents in astoral Regions of Ethiopia.Gezahegne Mamo, Gizachew Bayleyegn, Tesfaye Sisay Tessema, Mengistu Legesse, Girmay Medhin, Gunnar,Bjune, Fekadu Abebe, Gobena Ameni.PLoS One. 2011; 6(1): e15862.  Published online 2011 Jan 24. doi: 10.1371/journal.pone.0015862.PMCID: PMC3025912

[HTML] Bovine tuberculosis in a Nebraska herd of farmed elk and fallow deer: a failure of the tuberculin skin test and opportunities for serodiagnosis

[HTML] from hindawi.comW Waters, GE Stevens, MA Schoenbaum… - Veterinary medicine …, 2011 - hindawi.com... Isolates of M. bovis were identified by a combination of Ziehl-Neelsen acid-fast staining, nucleicacid probes (AccuProbe, Gen-Probe, San Diego, CA, USA), and spoligotyping (OcimumBiosolutions Ltd., Hyderabad, India). Identification of atypical Mycobacteria spp. ...Cited by 2 - Related articles - Cached - All 13 versions - Import into BibTeX

 [HTML] The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines …E Abadia, J Zhang, V Ritacco… - BMC infectious …, 2011 - biomedcentral.com... of hard to interpret spoligotypes and samples representative of the work flow, so we could evaluatethe benefits of a new platform regarding the classical one on these samples. Most centers hadperformed spoligotyping using commercial membranes (Ocimum, Hyderabad, India ...Cited by 3 - Related articles - Cached - All 26 versions - Import into BibTeX

Quality assurance for molecular epidemiology of tuberculosis methods in the mycobacterium reference laboratoryPJ Freidlin, D Goldblatt, H Kaidar-Shwartz… - … and Quality Assurance: …, 2011 - Springer... for spoli- gotyping results, standardization and reproducibility depend on the quality of thecommercial spoligotyping kit (Ocimum). ... Spoligotyping Pattern ... Both PETRA (a MIRU-VNTR lineagewith central asian spoligotypes, CAS) and Beijing clusters have been observed in other ...Related articles - All 3 versions - Import into BibTeX

DETERMINATION OF DRUG SUSCEPTIBILITY PATTERNS AND GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM KANPUR DISTRICT; …S Purwar, S Chaudhari, VM Katoch… - Infection, Genetics and …, 2011 - Elsevier... commercially available kit 126 (Isogen Biosciences , BV, Maarsen, The Netherlands now OcimumBiosolutions) and ... Owing to small sample size, the exact prevalence of various 272 spoligotypesand M ... Out of the isolates clustered by spoligotyping, all 280 were unique strains by ...Cited by 3 - Related articles - All 4 versions - Import into BibTeX

New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Mycobacterium tuberculosis</i> strains from Saudi ArabiaB Varghese, A Hillemann, DR Wijayanti… - Infection, Genetics and …, 2012 - Elsevier... Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typingand spoligotyping. Spoligotyping was performed as previously described (Kamerbeek et al., 1997)with the commercial kit (Ocimum Biosolutions, Hyderabad, India). ...All 2 versions - Import into BibTeX

High prevalence of subclinical tuberculosis in HIV-1-infected persons without advanced immunodeficiency: implications for TB screeningT Oni, R Burke, R Tsekela, N Bangani, R Seldon… - Thorax, 2011 - thorax.highwire.org... Lowenstein–Jensen slope cultures. The DNA was used to perform spoligotypingas previously described4 using a commercially available kit from Ocimum BiosolutionsBV, The Netherlands. The hybridisation patterns obtained ...Cited by 1 - Related articles - All 12 versions - Import into BibTeX

[HTML] Pathology of camel tuberculosis and molecular characterization of its causative agents in pastoral regions of Ethiopia

G Mamo, G Bayleyegn, TS Tessema, M Legesse… - PloS one, 2011 - dx.plos.org... Spoligotyping. Spoligotyping was performed as previously described by Kamerbeekand coauthors [22] and according to the spoligotype kit supplier's instructions (OcimumBiosolutions Company, Iisselstein, The Netherlands). ...Cited by 3 - Related articles - Cached - All 9 versions - Import into BibTeX

POPULATION-BASED INVESTIGATION OF FLUOROQUINOLONES RESISTANT TUBERCULOSIS IN RURAL EASTERN CHINA

Y Hu, B Mathema, W Wang, B Kreiswirth, W Jiang… - Tuberculosis, 2011 - Elsevier

... Spoligotyping was carried out using commercial kits from Isogen Bioscience BV (Maarssen, The Netherlands). 15 Strain family was determined by comparison of spoligotyping patterns with the SpoligDB4 database and assigned a spoligotyping international type (SIT). ...Cited by 2 - Related articles - All 4 versions - Import into BibTeX

[HTML] THE GUINEA-BISSAU FAMILY OF MYCOBACTERIUM TUBERCULOSIS COMPLEX REVISITED

R Groenheit, S Ghebremichael, J Svensson… - PLoS One, 2011 - dx.plos.org

... All isolates that were subjected to RFLP were also spoligotyped. ... Altogether, 414 isolates were available for spoligotyping, of which 336 isolates were grouped into 42 clusters ... Out of 229 isolates, 82 different spoligotypes were obtained, of which 168 (73.4%) were clustered into ...Cited by 4 - Related articles - Cached - All 5 versions - Import into BibTeX

GENETIC DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS IN MBARARA, SOUTH WESTERN UGANDA

J Bazira, M Matte, BB Asiimwe… - African health sciences, 2011 - ajol.info

... was performed as previously described29 using a commercially available kit (Isogen Bioscience BV ... Spoligotypes that did not match any existing pattern in the SpolDB4 database were ... tuberculosis within the Mbarara, we have used RD analysis and spoligotyping to characterize ...Related articles - All 8 versions - Import into BibTeX

FLUOROQUINOLONE RESISTANCE AMONG MYCOBACTERIUM TUBERCULOSIS STRAINS FROM KARACHI, PAKISTAN: DATA FROM COMMUNITY-BASED FIELD CLINICS

Y Rafiq, K Jabeen, R Hasan, S Jafri… - Antimicrobial agents …, 2011 - Am Soc Microbiol... was performed on all FQ-resistant strains using a commercially available kit provided by Isogen Life Science BV ... The spoligotypes were compared with the most prevalent M. tuberculosis subfamilies, as identified by the world spoligotyping database, SpolDB4.0, of ...Related articles - All 6 versions - Import into BibTeX

[HTML] CHARACTERISTICS OF PNCA MUTATIONS IN MULTIDRUG-RESISTANT TUBERCULOSIS IN TAIWAN

YC Chiu, SF Huang, KW Yu, YC Lee… - BMC infectious …, 2011 - biomedcentral.com

... Genotyping. All clinical isolates were genotyped by a commercial spoligotyping kit (Isogen Bioscience BV, Maarssen, Netherlands). The "Beijing strain" was defined as deletion from spacer 1 to spacer 34 in the direct repeat region and the presence of (at least 3) spacers 35-43. ...Related articles - Cached - All 11 versions - Import into BibTeX

[HTML] INITIAL PRESENTATIONS PREDICT MORTALITY IN PULMONARY TUBERCULOSIS PATIENTS-A PROSPECTIVE OBSERVATIONAL STUDY

 

JY Feng, WJ Su, YC Chiu, SF Huang, YY Lin… - PloS one, 2011 - dx.plos.org

... All clinical isolates were genotyped using a commercial spoligotyping kit (Isogen Bioscience BV, Maarssen, Netherlands). The “Beijing strain” was defined as a deletion from spacer 1 to spacer 34 in the direct repeat region and the presence of (at least 3) spacers in 35–43. ...Related articles - Cached - All 4 versions - Import into BibTeX

[HTML] MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS, BUENOS AIRES, ARGENTINA

X Gonzalo, M Ambroggi, E Cordova… - Emerging Infectious …, 2011 - ncbi.nlm.nih.gov

... DNA was extracted from cultures at this UK laboratory; spoligotyping was performed according to the manufacturer's instructions (Isogen Life Science, IJsselstein, the Netherlands); and data were analyzed with SPOTCLUST (http://cgi2.cs.rpi.edu/~bennek/SPOTCLUST.html). ...Related articles - All 4 versions - Import into BibTeX

CHARACTERISTICS OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS IN TAIWAN: A POPULATION-BASED STUDY

CW Chang, MH Wu, PC Chuang… - Infection, Genetics and Evolution, 2011 - Elsevier

... 12 13 Spoligotyping 14 A commercially available kit (Isogen Bioscience BV, Maarssen, The 15 Netherlands) was used as ... Even through spoligotyping results 3 revealed the genetic diversities of MDR M. tuberculosis isolates in Taiwan, 4 identical spoligotypes could not ...Cited by 2 - Related articles - All 2 versions - Import into BibTeX

MOLECULAR PATTERN OF MYCOBACTERIUM BOVIS ISOLATES AND ITS RELATIONSHIP WITH RISK FACTORS ASSOCIATED WITH THE PRESENCE OF BOVINE …

IF Padilla-Campos, JC Segura-Correa… - Tropical and …, 2011 - redalyc.uaemex.mx

... Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors. ... Spoligotyping was carried out following the protocol described by Kamerbeek et al ... sequences from M. tuberculosis and six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The ...Related articles - All 2 versions - Import into BibTeX

[HTML] FINE-TUNING THE SPACE, TIME, AND HOST DISTRIBUTION OF MYCOBACTERIA IN WILDLIFE

C Gortazar, MJ Torres, P Acevedo, J Aznar… - BMC …, 2011 - biomedcentral.com

... Typing. The M. bovis isolates were further characterized by spoligotyping [40]. The amplified product was detected by hybridization of the biotin-labelled PCR product onto spoligotyping membrane (Isogen Bioscience BV, Maarssen, The Netherlands). ...Cited by 6 - Related articles - Cached - All 19 versions - Import into BibTeX

MOLECULAR PATTERN OF MYCOBACTERIUM BOVIS ISOLATES AND ITS RELATIONSHIP WITH RISK FACTORS ASSOCIATED WITH THE PRESENCE OF …

IF Padilla, JC Segura, JC Rodriguez… - Tropical and …, 2011 - ccba.uady.mx

... Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors. ... Spoligotyping was carried out following the protocol described by Kamerbeek et al ... sequences from M. tuberculosis and six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The ...Related articles - All 2 versions - Import into BibTeX

EPIDEMIOLOGICAL TRENDS AND CLINICAL COMPARISONS OF< I> MYCOBACTERIUM</I>< I> TUBERCULOSIS</I> LINEAGES IN THAI TB MENINGITIS

K Faksri, F Drobniewski, V Nikolayevskyy, T Brown… - Tuberculosis, 2011 - Elsevier

... isolated from CSF samples collected from 1996 to 2004 with IS6110 RFLPs (120 RFLP patterns were available) and spoligotyping results (141 spoligotypes were available ... 27 Spoligotyping was performed according to the manufacturer's instructions (Isogen Bioscience BV ...Cited by 1 - Related articles - All 3 versions - Import into BibTeX

A NOVEL METHOD FOR THE RAPID AND PROSPECTIVE IDENTIFICATION OF BEIJING MYCOBACTERIUM TUBERCULOSIS STRAINS BY HIGH‐RESOLUTION MELTING ANALYSIS

M Alonso, Y Navarro, F Barletta… - Clinical …, 2011 - Wiley Online Library

... Thirty-two MTB strains (18 representatives of the Beijing genotype and 14 representatives of the non-Beijing genotype, according to their spoligotypes) were grown on ... Spoligotyping was performed in accordance with the manufacturer's instructions (Isogen, De Meern, the ...Cited by 3 - Related articles - All 3 versions - Import into BibTeX

QUALITY ASSURANCE FOR MOLECULAR EPIDEMIOLOGY OF TUBERCULOSIS METHODS IN THE MYCOBACTERIUM REFERENCE LABORATORY

PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… - … and Quality Assurance: …, 2011 - Springer

... 43 spacer spoligotyping was performed according to the manufacturer's instructions (Isogen/Ocimun) as described previously. ... 27 Spoligotyping Pattern ... Both PETRA (a MIRU-VNTR lineage with central asian spoligotypes, CAS) and Beijing clusters have been observed in other ...Related articles - All 3 versions - Import into BibTeX

EPIDEMIOLOGICAL ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED IN LODZ, POLAND

M Krawczyk, A Brzostek, A Gorna… - … of Tuberculosis and …, 2011 - ingentaconnect.com

... of van Embden et al.16 Spoligotyping was performed as described by Kame rbeek et al., using the Isogen Life Science kit ... Spoligotyping analysis showed that 184 (78.6%) of the strains were grouped in clusters and 50 (21.4%) strains possessed unique spoligotypes. ...Cited by 1 - Related articles - All 3 versions - Import into BibTeX

ONE YEAR NATIONWIDE EVALUATION OF 24-LOCUS MIRU-VNTR GENOTYPING ON SLOVENIAN< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES

U Bidovec-Stojkovic, M Zolnir-Dovc… - Respiratory Medicine, 2011 - Elsevier

... 4,9,20 Spoligotyping was performed using a commercially available kit (Isogen Bioscience BV ... identically defined by IS6110 RFLP and 24-locus MIRU-VNTR typing combined with spoligotyping. ...jointly defined by identical 24-locus MIRU-VNTR types and spoligotypes were only ...Related articles - All 3 versions - Import into BibTeX

MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX (MTBC) ISOLATED FROM CATTLE IN NORTHEAST AND NORTHWEST CHINA

Y Du, Y Qi, L Yu, J Lin, S Liu, H Ni… - Research in veterinary …, 2011 - Elsevier

... 2.5. Spoligotyping. Spoligotyping kits and Mini-blotter MN45 were obtained from Isogen Life Science Co. (Utrecht, the Netherlands). The Streptavidin-ß-peroxidase conjugate was produced by Roche Applied Science Co. (Penzberg, Germany). ...Cited by 3 - Related articles - All 3 versions - Import into BibTeX

[HTML] TRANSMISSION PATTERN OF DRUG-RESISTANT TUBERCULOSIS AND ITS IMPLICATION FOR TUBERCULOSIS CONTROL IN EASTERN RURAL CHINA

[HTML] from plos.org

Y Hu, B Mathema, W Jiang, B Kreiswirth, W Wang… - PloS one, 2011 - dx.plos.org

... Hybridized DNA was detected using an enhanced chemiluminescene kit from Isogen Bioscience BV ... b Probaiblity that the Spoligotyping pattern belongs to the clades. ... N, Bennett KP (2006) Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. ...Related articles - Cached - All 7 versions - Import into BibTeX

SUBLINEAGES OF BEIJING STRAIN OF MYCOBACTERIUM TUBERCULOSIS IN SRI LANKA

US Rajapaksa… - Indian Journal of Microbiology, 2011 - Springer

... Spoligotyping was carried out according to manufacturer's guidelines using a commercially available kit (Isogen, Maarsen MV, The Netherland). Fourteen iso-lates that confirmed to be Beijing strain were further characterised. ...Related articles - All 2 versions - Import into BibTeX

DETERMINATION OF DRUG SUSCEPTIBILITY PATTERNS AND GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM KANPUR DISTRICT; …

S Purwar, S Chaudhari, VM Katoch… - Infection, Genetics and …, 2011 - Elsevier

... 43 spacers was done with the help of a commercially available kit 126 (Isogen Biosciences , BV ... Owing to small sample size, the exact prevalence of various 272 spoligotypes and M.tuberculosis ... Out of the isolates clustered by spoligotyping, all 280 were unique strains by IS6110 ...Cited by 3 - Related articles - All 4 versions - Import into BibTeX

MOLECULAR EPIDEMIOLOGY OF HUMAN AND ANIMAL TUBERCULOSIS IN IBADAN, SOUTHWESTERN NIGERIA

AO Jenkins, SIB Cadmus, EH Venter… - Veterinary …, 2011 - Elsevier

... Spoligotyping167 Spoligotyping confirmed the species identification of 19 M. tuberculosis and 13 M ... None of these CAM 173 variant spoligotypes were found in the spolDB4 database and were ...All M. tuberculosis strains 175 from PTB patients that were spoligotyped had identical ...Cited by 1 - Related articles - All 5 versions - Import into BibTeX

GENETIC DIVERSITY OF THE MYCOBACTERIUM TUBERCULOSIS BEIJING FAMILY BASED ON IS6110, SNP, LSP AND VNTR PROFILES FROM THAILAND

K Faksri, F Drobniewski, V Nikolayevskyy… - Infection, Genetics and …, 2011 - Elsevier

... Eighty MTB Beijing isolates from CSF samples with IS6110 RFLPs (69 RFLP patterns were available) and spoligotyping results (all 80 spoligotypes were available ... Spoligotyping wasperformed according to the manufacturer s instructions (Isogen Bioscience BV ...Cited by 2 - Related articles - All 3 versions - Import into BibTeX

[PDF] 骨结核临床分离菌株基因分型的特点

李文胜， 刘毅， 孙勇， 田苗， 刘忠泉… - 中华临床医师杂志 ( …, 2011 - clinicmed.net

... TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自TaＫaRa 公司。凝胶成像仪购自FOTO DＹNE 公司。 Spoligotyping 试 剂盒以及Miniblotter MN４５为荷兰Isogen Biosｃienｃe 公司产品。 链霉亲和素为Sigam 公司产品。 化学发光 ...Related articles - View as HTML - All 3 versions - Import into BibTeX

MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS IN THE UNITED ARAB EMIRATES

A Saedi… - 2011 - qmro.qmul.ac.uk

... 130 3.2.11 Identification of the UAE isolates by spoligotyping..... 132 3.2.12 Comparison between MIRU-typed and spoligotyped families in association with katG463/gyrA95 groupings ..... ...Related articles