Spoligotyping

Mapmygenome acquired the rights to sell Spoligotyping from Ocimum Biosolutions in 2014. 

Ocimum acquired the spoligotyping kit through an acquisition of the Biomolecules division of Isogen Lifesciences based in The Netherlands. Since the acquisition in 2006, Ocimum has continued to invest in additional R&D and is in the process of adding several new kits to its portfolio.

As with other products, below is a chronological publication reference to the spoligotyping kit. The earliest publication for this product is dated way back in 1997. Many publications will refer to Isogen as that was the name of our subsidiary. Recently we changed the name back to Ocimum Biosolutions but we continue to find references to Isogen.

With the large number of references of over 400 and from labs across the world, the spoligotyping kit has been validated for over 15 years now giving confidence to researchers across the globe using it.

 

2022

 

Era of TB elimination: Growing need to understand diversities of Mycobacterium tuberculosis lineages!

A Tejashree, PA Mahesh, MK Karthik, A Azeem… - Indian Journal of …, 2022 - Elsevier

… The Spoligotyping was carried out by amplifying the whole DR region using the commercially available kit (Mapmygenome, Hyderabad) according to a standardized method using the …

 

[HTML] Molecular Epidemiology and Polymorphism Analysis in Drug-Resistant Genes in M. tuberculosis Clinical Isolates from Western and Northern India

V Rana, N Singh, C Nikam, P Kambli… - Infection and Drug …, 2022 - ncbi.nlm.nih.gov

… kit (Ocimum Biosolutions, Hyderabad). The standardized spoligotyping protocol has

been described previously. Briefly, the cycling conditions used for spoligotyping were: initial 

 

[HTML] Spoligotyping of Clinical Isolates of Mycobacterium tuberculosis Complex Species in the Oromia Region of Ethiopia

B Hussien, A Zewude, B Wondale, A Hailu… - Frontiers in Public …, 2022 - ncbi.nlm.nih.gov

… (29) and as per the spoligotype kit supplier's instructions (Ocimum Biosolutions, Ijsselstein, The Netherlands). DNA from known strains of M. bovis SB1176 and M. …

 

 

 

2021

 

Bovine tuberculosis in Taiwan, 2008–2019

TH Chan, CS Huang, C Tu, R Jou - … and Emerging Diseases, 2021 - Wiley Online Library

… Spacer oligonucleotide typing (spoligotyping) A commercially available kit (Ocimum Biosolutions, … that was covalently precoated with a set of 43 spacer oligonucleotides derived from the …

 

Whole genome sequencing analysis of multidrug-resistant tuberculosis in Singapore, 2006–2018

CBE Chee, LKY Lim, RTH Ong, LH Sng, LY Hsu… - European Journal of …, 2021 - Springer

… Spoligotyping and MIRU-VNTR methods identified 108 patients in 24 clusters. The Beijing

spoligotype … One cluster belonged to the EAI spoligotype and another to the H spoligotype. …

 

[HTML] Multispacer Sequence Typing for Mycobacterium bovis Genotyping

ÉB Sales, AA Fonseca Jr, CM Gonçalves… - Frontiers in Veterinary …, 2021 - ncbi.nlm.nih.gov

… Among the remaining techniques, spacer oligonucleotide typing (spoligotyping; based on … (41) using spolygotyping kit by Ocimum Biosolutions. The identification of individual 

 

 

2020

 

[HTML] Spoligotyping and drug sensitivity of Mycobacterium tuberculosis isolated from pulmonary tuberculosis patients in the Arsi Zone of southeastern Ethiopia

B Haile, K Tafess, A Zewude, B Yenew, G Siu… - New Microbes and New …, 2020 - Elsevier

… (Ocimum Biosolutions, Ijsselstein, the Netherlands). The direct repeat (DR) region was amplified by PCR using oligonucleotide … of 43 immobilized oligonucleotides, each corresponding …

 

DRUG RESISTANCE OF MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATES FROM NEW TB CASES IN KAZAKHSTAN

U Kozhamkulov, A Akhmetova, S Rakhimova… - 2020 - nur.nu.edu.kz

… drug resistance, genotyping and whole genome sequencing. Methods: 192M.tuberculosis … available kit “Ocimum Biosolutions Inc”. The whole genome sequencing was carried out on …

 

[HTML] Spoligotype Variation of Mycobacterium tuberculosis Strains Prevailing in Korea

SE Song, DH Kim, SH Kim, MS Park… - Canadian Journal of …, 2020 - hindawi.com

… Spoligotyping was performed as described by the manufacturer (Ocimum Biosolutions). E binary spoligotype formats were compared with the SITVIT2 database …

 

2019

[PDF] UPDATE ON VIRULENCE FACTORS IN MYCOBACTERIA

R Brosch - Инфекция и иммунитет, 2019 - iimmun.ru… cially available kit (Mapmygenome India Ltd., Madhapur, India) according to the manufacturer's protocol …Spoligotypes were obtained for 127 (97.8%) isolates … When spoligotyping and MIRU-VNTR typing was used in com- bination, only tw o, out of 130 isolates, clustered both in …[HTML] plos.org

 

[HTML] Genetic diversity of Mycobacterium tuberculosis strains circulating in Botswana

T Mogashoa, P Melamu, SD Ley, EM Streicher… - PloS one, 2019 - journals.plos.org… A commercially available spoligotyping kit (Mapmygenome, Hyderabad India) was used for amplification, hybridization and detection of … RIF or to both INH and RIF and which clustered according to spoligotyping (n = 65 … A total of 458/461 isolates were successfully spoligotyped …

 

Genetic diversity of Mycobacterium tuberculosis isolates causing pulmonary and extrapulmonary tuberculosis in the capital of Iran

S Hadifar, L Shamkhali, MK Kamakoli… - Molecular phylogenetics …, 2019 - Elsevier… All M.tb isolates were analyzed with Spoligotyping commercial kit (Mapmygenome Genomics company … 1. Distribution of M.tb genotypes, SIT and corresponding spoligotype patterns of all … There was 100% concordance between Spoligotyping and MIRU-VNTR data (except for …

 

Association of ubiA mutations and high-level of ethambutol resistance among Mycobacterium tuberculosis Thai clinical isolates

O Tulyaprawat, A Chaiprasert, P Chongtrakool… - Tuberculosis, 2019 - Elsevier… Spoligotyping was performed according to the manufacturer's instructions (Mapmygenome, India) [17] … The octal code format of each strain was entered to assign Spoligotype International Type (SIT) and phylogenetic clade by using SITVITWEB, a database of the Pasteur …[HTML] frontiersin.org

 

Bayesian Assessment of the Accuracy of a PCR-Based Rapid Diagnostic Test for Bovine Tuberculosis in Swine

S Barandiaran, MS Perez Aguirreburualde… - Frontiers in veterinary …, 2019 - frontiersin.org… Spoligotyping was carried out using the spoligotyping kit (Mapmygenome India) … remaining 21% culture and PCR-negative samples (ie, 4.9% of all the samples) were M. avium complex (IS1245+)-positive (Table 3). All IS6110-positive samples showed spoligotypes that were …

 

Prevalence of Mycobacterium bovis infection in traditionally managed cattle at the wildlife-livestock interface in South Africa in the absence of control measures

PR Sichewo, EMC Etter, AL Michel - Veterinary Research …, 2019 - Springer… Spoligotyping assay was performed using a commercially available kit (Mapmygenome India Ltd.) to … Spoligotyping confirmed the species identification of the 12 isolates from cattle and 22 from … the isolates showed identical profiles belonging to the SB 0130 spoligotype, which is …Related articles All 2 versions

 

Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

PAM Carneiro, TN Pasquatti, H Takatani… - Veterinary Medicine …, 2019 - Wiley Online Library… of spacer sequences, using a miniblotter according to the manufacturer's instructions (MapMyGenome) … The spoligotyping was performed on 35 M. bovis isolates and six types of … The other municipalities presented only one spoligotype each, Manacapuru (SB0822), Itacoatiara …

 

MOLECULAR TYPING OF MYCOBACTERIUM KANSASII—A GLOBAL PERSPECTIVE

Z Bakuła, F Arias, E Bachiyska, E Borroni… - Инфекция и …, 2019 - iimmun.ru… cially available kit (Mapmygenome India Ltd., Madhapur, India) according to the manufacturer's protocol … Spoligotypes were obtained for 127 (97.8%) isolates … When spoligotyping and MIRU-VNTR typing was used in com- bination, only tw o, out of 130 isolates, clustered both in …  Cited by 1 Related articles 

 

[HTML] Occurrence of disputed rpoB mutations among Mycobacterium tuberculosis isolates phenotypically susceptible to rifampicin in a country with a low incidence …

NM Al-Mutairi, S Ahmad… - BMC infectious …, 2019 - bmcinfectdis.biomedcentral.com

… Molecular detection of resistance for ethambutol and pyrazinamide and fingerprinting by spoligotyping were also performed for isolates with an rpoB mutation. Results … Spoligotyping showed that the isolates belonged to different M. tuberculosis lineages. Conclusions …  Cited by 5 Related articles All 8 versions 

 

Usefulness of 3'- 5' IS6110-RFLP genotyping and spoligotyping of Mycobacterium tuberculosis isolated in a tertiary hospital: a retrospective study detecting …

SM Almeida, AC Malaspina, CQF Leite… - Revista do Instituto de …, 2019 - SciELO Brasil

… from Ocimum Biosolutions Ltda (Hyderabad, India). An overestimation of the number of

potentially related isolates is a possibility, as the discriminatory power of spoligotyping is lower …

 

Whole-genome sequencing of two extensively drug-resistant Mycobacterium tuberculosis isolates from India

SB Rufai, S Singh - Microbiology Resource Announcements, 2019 - Am Soc Microbiol

… by molecular characterization by application of a spoligotyping technique using a commercial kit (Ocimum Biosolutions, India). The spoligotyping method, which was done per the …

 

Genotype diversity and distribution of Mycobacterium bovis from livestock in a small, high-risk area in northeastern Sicily, Italy.Cinzia Marianelli, Benedetta Amato, Maria Beatrice Boniotti, Maria Vitale, Flavia Pruiti Ciarello, Maria Lodovica Pacciarini, Vincenzo Di Marco Lo Presti.PLoS Negl Trop Dis. 2019 Jul; 13(7): e0007546. Published online 2019 Jul 15. doi: 10.1371/journal.pntd.0007546. PMCID: PMC6658142

Genetic diversity and drug susceptibility profiles of Mycobacterium tuberculosis obtained from  Saint Peter’s TB specialized Hospital, Ethiopia.Delesa Damena, Samuel Tolosa, Milkessa Hailemariam, Aboma Zewude, Adane Worku, Biruk Mekonnen, Temesgen Mohammed, Addisu Admasu, Emile R. Chimusa, Adane  Mihret, Tamrat Abebe, Gobena Ameni.PLoS One. 2019; 14(6): e0218545.  Published online 2019 Jun 24.  doi: 10.1371/journal.pone.0218545. PMCID: PMC6590806

Exploring the “Latin American Mediterranean” family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela. Chyntia Carolina Díaz Acosta, Graciela Russomando, Norma Candia, Viviana Ritacco, Sidra E. G. Vasconcellos, Marcia de Berrêdo Pinho  Moreira, Nilda J. de Romero, Nora Morcillo, Jacobus Henri De Waard, Harrison Magdinier Gomes, Philip Noel Suffys.BMC Microbiol. 2019; 19: 131. Published online 2019 Jun 13. doi: 10.1186/s12866-019-1479-6. PMCID: PMC6567603

Genetic diversity and drug susceptibility patterns of the Mycobacterium tuberculosis complex in Yunnan, China. Ruolan Bai, Shuijing Chi, Xiaofei Li, Xiting Dai, Zhenhua Ji, Miaomiao Jian, Yunfeng  Bi, Feng Wang, Zhe Ding, Lisha Luo, Taigui chen, Aihua Liu, Fukai Bao,Biosci Rep. 2019 May 31; 39(5): BSR20181746.  Pre-published online 2019 Apr 15. Published online 2019 May 31. doi:  10.1042/BSR20181746. PMCID: PMC6542976

Demographic risk factors for extra-pulmonary tuberculosis among adolescents and adults in Saudi ArabiaHawra Al-Ghafli, Bright Varghese, Mushira Enani, Abdulrahman Alrajhi, Sameera Al Johani, Ali  Albarrak, Sahar Althawadi, Noura Elkizzi, Sahal Al Hajoj,PLoS One. 2019; 14(3): e0213846.  Published online 2019 Mar 27. doi: 10.1371/journal.pone.0213846. PMCID: PMC6436801

Tuberculosis Caused by Mycobacterium bovis in a Sheep Flock Colocated with a Tuberculous Dairy Cattle Herd in Central Ethiopia.Benti Deresa Gelalcha, Aboma Zewude, Gobena Ameni,J Vet Med. 2019; 2019: 8315137. Published online 2019 Mar 3. doi: 10.1155/2019/8315137. PMCID: PMC6421010

Active and latent tuberculosis among inmates in La Esperanza prison in Guaduas, Colombia Julio Guerra, Daniel Mogollón, Deccy González, Ricardo Sanchez, Zulma Vanessa Rueda, Carlos A. Parra-López, Martha Isabel Murcia, PLoS One. 2019; 14(1): e0209895. Published online 2019 Jan 25. doi:  10.1371/journal.pone. 0209895. PMCID: PMC6347203

 

2018

Spoligotyping for Identification of Pulmonary Tuberculosis with Type 2 Diabetes Mellitus Profiles in Warangal, TS

S Tanniru, S Asha, M Jojula - Anti-Infective Agents, 2018 - ingentaconnect.com… culosis) and MDR TB (Multi Drug Resistance Tuberculosis) and different Spoligotypes are represented … Molecu- lar methods like Spoligotyping are used to differentiate and identify species of … & molecular identification methods, we found most prevalent Spoligotype pattern which …Related articles

 

Clofazimine drug susceptibility testing for Mycobacterium tuberculosis: the case of using the right diluent

LH Sng, JWL Peh, MTL Kee, RTH Ong, CW Wong… - Pathology, 2018 - Elsevier… The isolates were identified as MTB using the BD MGIT TBc Identification Test (TBcID; Becton Dickinson, USA) and strain typed by spoligotyping (Ocimum Biosolutions, India). DST for clofazimine had been carried out using the MGIT method 10 at a concentration of 0.5 μg/mL …

[HTML] Investigation of a cluster of multi-drug resistant tuberculosis in a high-rise apartment block in Singapore

ZJM Ho, CBE Chee, RTH Ong, LH Sng… - International Journal of …, 2018 - Elsevier… elements [Figure 1]. The isolates were determined in May 2016 to have identical spoligotype(Ocimum Biosolutions, Hyderabad) and 24-loci Mycobacterial Interspersed Repetitive Unit –Variable Number of Tandem Repeat (MIRU-VNTR: Genoscreen, France) profiles (Chee et …Cited by 3 Related articles All 6 versions

 

[PDF] Comparison of PFGE, IS6110-RFLP, and 24-Locus MIRU-VNTR for Molecular Epidemiologic Typing of Mycobacterium tuberculosis Isolates with Known …

S Jeon, N Lim, S Park, M Park… - Journal of microbiology …, 2018 - pdfs.semanticscholar.org… Spoligotyping Spoligotyping was performed to identify the lineages of the isolates used in this …The spoligotypes were classified according to SITVITWEB clade assignments (http://www.pasteru-guadeloupe.fr.  There was no spoligotype match found in the SITVIT database for …  Cited by 1 Related articles All 6 versions 

 

[CITATION] Mycobacterium bovis Infection in a Human in Malaysia

S Suraiya, NI Farakhin - Clinical Microbiology Newsletter, 2018 - Elsevier

[HTML] Molecular clustering of patients with Mycobacterium tuberculosis strains cultured from the diabetic and non-diabetic newly diagnosed TB positive cases

JW Mburu, L Kingwara, M Esther, N Andrew - Journal of Clinical …, 2018 - Elsevier… as described by Kamerbeek et, al. [9]. Spoligotyping was performed using the spoligotype kit (Ocimum bio solutions company, Netherlands) according to the manufacturer's instructions. The direct repeats (DR) were amplified …Related articles All 3 versions

 

Multi-and extensively drug resistant Mycobacterium tuberculosis in South Africa: a molecular analysis of historical isolates

NE Maningi, LT Daum, JD Rodriguez… - Journal of clinical …, 2018 - Am Soc MicrobiolCited by 2 Related articles All 6 versions

 

Annotated genome sequences of 16 lineage 4 Mycobacterium tuberculosis strains from guatemala

JW Saelens, D Lau-Bonilla, A Moller… - Genome …, 2018 - Am Soc Microbiol… 5 Prime GmbH, Germany). Spoligotyping was carried out using the spoligotyping kit and protocol from Isogen Biosolutions (Ocimum Biosolutions Ltd., India), identifying the isolates as lineage 4 strains. Paired-end 50-bp reads …  Cited by 1 Related articles All 5 versions

 

[HTML] Risk factors associated with cluster size of Mycobacterium tuberculosis (Mtb) of different RFLP lineages in Brazil

RL Peres, SA Vinhas… - BMC infectious …, 2018 - bmcinfectdis.biomedcentral.com… Of the isolates spoligotyped, 448 (52%) were classified as LAM and 412 (48%) as … Isolates were also submitted to spoligotyping by a commercial kit (Ocimum Biosolutions Inc., India … a standard protocol [17, 18], allowing the classification of strains into spoligotype-based families …  Cited by 3 Related articles All 9 versions 

 

[HTML] Evidence for Host-Bacterial Co-evolution via Genome Sequence Analysis of 480 Thai Mycobacterium tuberculosis Lineage 1 Isolates

P Palittapongarnpim, P Ajawatanawong… - Scientific …, 2018 - ncbi.nlm.nih.gov… of the single nucleotide variant (SNV) phylogenetic tree and the clades defined by spoligotyping revealed some monophyletic clades representing EAI2_MNL, EAI2_NTM and EAI6_BGD1 spoligotypes. Our work demonstrates that ambiguity in spoligotype assignment could be …  Cited by 5 Related articles All 11 versions

 

[HTML] Genotyping and molecular detection of multidrug-resistant Mycobacterium tuberculosis among tuberculosis lymphadenitis cases in Addis Ababa, Ethiopia

O Zewdie, A Mihret, T Abebe, A Kebede, K Desta… - New microbes and new …, 2018 - Elsevier… Spoligotyping was carried out using a commercially available kit from Ocimum Biosolutions (UK …Spoligotype patterns of each strain were prepared in binary and octal format and entered intothe recently released international spoligotyping database SITVITWEB [12] for …Cited by 2 Related articles All 6 versions

 

[HTML] Impact of Mycobacterium tuberculosis complex lineages as a determinant of disease phenotypes from an immigrant rich moderate tuberculosis burden …

B VargheseM Enani, A Alrajhi… - Respiratory …, 2018 - respiratory-research.biomedcentral …… according to the manufacturer's instructions. Primary genotyping of all isolates were carried out by using the commercially available spoligotyping assay (Ocimum Biosolutions, Hyderabad, India). The 24 loci based MIRU-VNTR …  Cited by 1 Related articles All 9 versions 

 

Genotypes of Mycobacterium bovis strains isolated from domestic animals and wildlife in Canada in 1985–2015

O Andrievskaia, C Turcotte, G Berlie-Surujballi… - Veterinary …, 2018 - Elsevier… Isolates were spoligotyped as described by Kamerbeek et al … The spacer sequences contained in the DR locus were detected by hybridization onto spoligotyping membranes using … in the DR locus was represented in a binary code, and translated into spoligotype octal codes …  Cited by 4 Related articles All 5 versions

 

[HTML] Association of gyrA and rrs gene mutations detected by MTBDR sl V1 on Mycobacterium tuberculosis strains of diverse genetic background from India

SB Rufai, J Singh, P Kumar, P Mathur, S Singh - Scientific reports, 2018 - nature.com… Spoligotyping was performed using the commercially available kit (Ocimum Biosolutions,Hyderabad … The hybridization patterns obtained in the binary format were transformed to an octal code for assessment with the spoligotype patterns using SITVIT2 database …Cited by 1 Related articles All 6 versions

 

 

[HTML] Bacterial risk factors for treatment failure and relapse among patients with isoniazid resistant tuberculosis

PVK ThaiDTM HaNT Hanh… - BMC infectious …, 2018 - bmcinfectdis.biomedcentral.com..Drug resistant tuberculosis (TB) is increasing in prevalence worldwide. Treatment failure and relapse is known to be high for patients with isoniazid resistant TB treated with standard first line regimens. However, risk factors for unfavourable outcomes and the optimal treatment regimen …  Cited by 4 Related articles All 11 versions 

 

 

Genetic Diversity of Mycobacterium tuberculosis Isolates in the Metropolitan Area of Rome

A Cannas, S Camassa, M Sali, O Butera, A Mazzarelli… - Chemotherapy, 2018 - karger.com… All strains were analyzed by the standardized spoligotyping technique as previously described [18] using a commercial kit (Ocimum Biosolutions, India) … Spoligotype patterns were represented as octal codes and entered in the SITVIT2 proprietary database of the …Related articles All 6 versions

 

Comparison of methods and samples used in the diagnosis of childhood PTB and characterization of mycobacterium tuberculosis isolates

AE Ogunbayo - 2018 - scholar.ufs.ac.za… Polymorphism..... 42 2.7.2.2. Spoligotyping ..... 43 2.7.2.3 …3.10.9.1. Spoligotyping ..... 60 3.10.9.2. MIRU … Related articles All 3 versions 

 

[PDF] 8. Molecular typing of Mycobacterium tuberculosis complex isolates

G Skenders, D van Soolingen - Handbook on tuberculosis laboratory …, 2018 - tbonline.info… It is, however, possible to use spoligotyping as a screening method in typing (in the vast … On the other hand, when spoligotype patterns are identical, then no conclusion can be drawn … Protocol: PCR: 1. Reconstitute dry primers Dra and DRb (Ocimum Biosolutions, India) in 2.2 ml …

 

[HTML] Mycobacterium africanum (Lineage 6) shows slower sputum smear conversion on tuberculosis treatment than Mycobacterium tuberculosis (Lineage 4) in …

B Diarra, M Kone, ACG Togo, YDS Sarro, AB Cisse… - PloS one, 2018 - journals.plos.org… or 5M. Spoligotyping was performed on boiled bacterial lysates using a commercially available kit (Ocimum)[22] or by in-house prepared membrane. Data entry from the film was verified by a second assessor. Lineage classification …

 

The effect of combining natural terpenes and antituberculous agents against reference and clinical Mycobacterium tuberculosis strains

E Sieniawska, R Sawicki, M Swatko-Ossor… - Molecules, 2018 - mdpi.com… It was described in most of the common highly fragrant herbs and spices, among which Humulus lupulus, Laurus nobilis, Thymus vulgaris, Cymbopogon schoenanthus or Ocimum basilicum contain high levels of this compound [17] … 2. Results. 2.1. Spoligotyping …  Cited by 7 Related articles All 8 versions 

 

Molecular Characterization of Drug-Resistant Mycobacterium Tuberculosis and Evaluation of the Performance of Genexpert Mtb/Rif® Assay Using Urine Specimen to …

E Abera - 2018 - 213.55.95.56… 113 Figure 5.1: Radial UPGMA tree based on the copy numbers of MIRU-VNTR 24-loci and Spoligotyping, and it shows the pattern of drug resitance lineage and clustering of each genotype … 24 MIRU-VNTR and spoligotype. Data were analyzed using SPSS version 23 …

 

Impact of Mycobacterium tuberculosis complex lineages as a determinant of disease phenotypes from an immigrant rich moderate tuberculosis burden country, Bright Varghese, Mushira Enani, Abdulrahman Alrajhi, Sameera Al Johani, Ali Albarak, Sahar Althawadi, Noura Elkhizzi, Hawra AlGhafli, Mohammed Shoukri, Sahal Al - Hajoj, Respir Res. 2018; 19: 259. Published online 2018 Dec 27. doi: 10.1186/s12931-018-0966-x,PMCID: PMC6307224

Mycobacterium africanum (Lineage 6) shows slower sputum smear conversion on tuberculosis treatment than Mycobacterium tuberculosis (Lineage 4) in Bamako, Mali.Bassirou Diarra, Mahamadou Kone, Antieme Combo Georges Togo, Yeya dit Sadio Sarro, Aissata Boubakar Cisse, Amadou Somboro, Boureima Degoga, Mohamed Tolofoudie, Bourahima Kone, Moumine Sanogo, Bocar Baya, Ousmane Kodio, Mamoudou Maiga, Michael Belson, Susan Orsega, Meryam Krit, Sounkalo Dao, Ibrahim Izétiegouma Maiga, Robert L. Murphy, Leen Rigouts, Seydou Doumbia, Souleymane Diallo, Bouke Catherine de Jong PLoS One. 2018; 13(12): e0208603. Published online 2018 Dec 12. doi: 10.1371/journal.pone.0208603. PMCID: PMC6291124

Spoligotype‐based population structure of Mycobacterium tuberculosis in the Jimma Zone, southwest Ethiopia. Gemeda Abebe, Ketema Abdissa, Kedir Abdella, Mulualem Tadesse, Adane Worku, Gobena Ameni, Microbiology open. 2019 Jun; 8(6): e00744. Published online 2018 Oct 15. doi: 10.1002/mbo3.744. PMCID: PMC6562125

Evidence for Host-Bacterial Co-evolution via Genome Sequence Analysis of 480 Thai Mycobacterium tuberculosis Lineage 1 Isolates. Prasit Palittapongarnpim, Pravech Ajawatanawong, Wasna Viratyosin, Nat Smittipat, Areeya Disratthakit, Surakameth Mahasirimongkol, Hideki Yanai, Norio Yamada, Supalert Nedsuwan, Worarat Imasanguan, Pacharee Kantipong, Boonchai Chaiyasirinroje, Jiraporn Wongyai, Licht Toyo-oka, Jody Phelan, Julian Parkhill, Taane G. Clark, Martin L. Hibberd, Wuthiwat Ruengchai, Panawun Palittapongarnpim, Tada Juthayothin, Sissades Tongsima, Katsushi Tokunaga Sci Rep. 2018; 8: 11597. Published online 2018 Aug 2. doi: 10.1038/s41598-018-29986-3. PMCID: PMC6072702

Association of gyrA and rrs gene mutations detected by MTBDRsl V1 on Mycobacterium tuberculosis strains of diverse genetic background from India.Syed Beenish Rufai, Jitendra Singh, Parveen Kumar, Purva Mathur, Sarman Singh. Sci Rep. 2018; 8: 9295. Published online 2018 Jun 18. doi: 10.1038/s41598-018-27299-z. PMCID: PMC6006251

Multi- and Extensively Drug Resistant Mycobacterium tuberculosis in South Africa: a Molecular Analysis of Historical Isolates. Nontuthuko E. Maningi, Luke T. Daum, John D. Rodriguez, Halima M. Said, Remco P. H. Peters, John Osei Sekyere, Gerald W. Fischer, James P. Chambers, P. Bernard Fourie J Clin Microbiol. 2018 May; 56(5): e01214-17.  Pre-published online 2018 Mar 7. Published online 2018 Apr 25. doi: 10.1128/JCM. 01214-17.PMCID: PMC5925707

Annotated Genome Sequences of 16 Lineage 4 Mycobacterium tuberculosis Strains from Guatemal Joseph W. Saelens, Dalia Lau-Bonilla, Anneliese Moller, Ana M. Xet-Mull, Narda Medina, Brenda Guzmán, Maylena Calderón, Raúl Herrera, Jason E. Stout, Eduardo Arathoon, Blanca Samayoa, David M. Tobin Genome Announc. 2018 Feb; 6(7): e00024-18. Published online 2018 Feb 15. doi: 10.1128/genomeA.00024-18. PMCID: PMC5814500

Risk factors associated with cluster size of Mycobacterium tuberculosis (Mtb) of different RFLP lineages in Brazil. Renata Lyrio Peres, Solange Alves Vinhas, Fabíola Karla Correa Ribeiro, Moisés Palaci, Thiago Nascimento do Prado, Bárbara Reis-Santos, Eliana Zandonade, Philip Noel Suffys, Jonathan E. Golub, Lee W. Riley, Ethel Leonor Maciel,BMC Infect Dis. 2018; 18: 71. Published online 2018 Feb 8. doi: 10.1186/s12879 018-2969-0. PMCID: PMC5806441

The Effect of Combining Natural Terpenes and Antituberculous Agents against Reference and Clinical Mycobacterium tuberculosis Strains,Elwira Sieniawska, Rafal Sawicki, Marta Swatko-Ossor, Agnieszka Napiorkowska, Agata Przekora, Grazyna Ginalska, Ewa Augustynowicz-Kopec Molecules. 2018 Jan; 23(1): 176.  Published online 2018 Jan 15. doi: 10.3390/molecules23010176.PMCID: PMC6017631

Genetic diversity and distribution of Mycobacterium tuberculosis genotypes in Limpopo, South Africa.N. T. C. Maguga-Phasha, N. S. Munyai, F. Mashinya, M. E. Makgatho, E. F. Mbajiorgu .BMC Infect Dis. 2017; 17: 764.  Published online 2017 Dec 12. doi: 10.1186/s12879-017-2881-z PMCID: PMC5727936

Genotyping and molecular detection of multidrug-resistant Mycobacterium tuberculosis among tuberculosis lymphadenitis cases in Addis Ababa, Ethiopia.O. Zewdie, A. Mihret, T. Abebe, A. Kebede, K. Desta, A. Worku, G. Ameni,New Microbes New Infect. 2018 Jan; 21: 36–41.  Published online 2017.Oct 24.oi: 10.1016/j.nmni.2017.10.009.PMCID: PMC5901530

PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping. B. Molina-Moya, A. Lacoma, N. García-Sierra, S. Blanco, L. Haba, S. Samper, J. Ruiz-Manzano, C. Prat, C. Arnold, J. Domínguez,Sci Rep. 2017; 7: 6777.  Published online 2017 Jul 28. doi: 10.1038/s41598-017-06760-5,PMCID: PMC5533701

First Insight into the Fluoroquinolone and Aminoglycoside Resistance of Multidrug-Resistant Mycobacterium tuberculosis in Saudi Arabia,Bright Varghese, Sahal Al-Hajoj,Am J Trop Med Hyg. 2017 May 3; 96(5): 1066–1070.  doi: 10.4269/ajtmh.16-0579Correction in: Am J Trop Med Hyg. 2018 Feb; 98(2): 634. PMCID: PMC5417196

2017

 

Strains of Mycobacterium tuberculosis Transmitting Infection in Brazilian Households and Those Associated with Community Transmission of Tuberculosis.Solange Alves Vinhas, Edward C. Jones-López, Rodrigo Ribeiro Rodrigues, Mary Gaeddert, Renata Lyrio Peres, Patricia Marques-Rodrigues, Paola Poloni Lobo de Aguiar, Laura Forsberg White, David Alland,Padmini Salgame, David Hom, Jerrold J. Ellner, Reynaldo Dietze, Lauren F. Collins, Elena Shashkina, Barry Kreiswirth, Moisés Palaci .Tuberculosis (Edinb) Author manuscript; available in PMC 2018 May 1.Published in final edited form as: Tuberculosis (Edinb). 2017 May; 104: 79–86. Published online 2017 Mar 16.  doi: 10.1016/j.tube.2017.03.003.PMCID: PMC5716464

 

Molecular typing of Mycobacterium tuberculosis complex isolated from pulmonary tuberculosis patients in central Ethiopia.Zufan Bedewi, Adane Worku, Yalemtsehay Mekonnen, Getnet Yimer, Girmay Medhin, Gezahegne Mamo, Rembert Pieper, Gobena Ameni,BMC Infect Dis. 2017; 17: 184.  Published online 2017 Mar 1. doi: 10.1186/s12879-017-2267-2.PMCID: PMC5333391

 

[HTML] Trends in spoligotype patterns of Mycobacterium tuberculosis strains in central India

P Desikan, DS Chauhan, N Panwalkar… - Indian journal of medical …, 2017 - ijmm.org… Table 1: Year wise occurrence of spoligotype lineages over the period of five years Click here to view … Comparison of spoligotyping, mycobacterial interspersed repetitive units typing and IS6110-RFLP in a study of genotypic … Predominant tuberculosis spoligotypes, Delhi, India …Cited by 1 Related articles All 4 versions

 

[HTML] Molecular typing of Mycobacterium tuberculosis complex isolated from pulmonary tuberculosis patients in central Ethiopia

Z Bedewi, A Worku, Y Mekonnen… - BMC infectious …, 2017 - bmcinfectdis.biomedcentral.com… Isolates that were positive for M. tuberculosis by RD9 PCR were further characterized by spoligotyping following the procedure described by Kamerbeeket al [17] and by observing the instructions of the spoligotype kit supplier (Ocimum Biosolutions Company, Iisselstein, and the …

 

 

First Insight into the Fluoroquinolone and Aminoglycoside Resistance of Multidrug-Resistant Mycobacterium tuberculosis in Saudi Arabia

B VargheseS Al-Hajoj - The American journal of tropical medicine and …, 2017 - ASTMH… Genotyping of MDR-TB isolates and data analysis. All the isolates reported with resistance to either amino- glycosides or fluoroquinolone were subjected to spoligotyping (Ocimum Biosolutions, Hyderabad, India) and 24-loci-based …

Cited by 1 Related articles All 4 versions

 

 

[HTML] Genetic diversity and distribution of Mycobacterium tuberculosis genotypes in Limpopo, South Africa

NTC Maguga-Phasha… - BMC infectious …, 2017 - bmcinfectdis.biomedcentral.com… They were spoligotyped and data analysed using the international spoligotyping database 4 (SpolDB4). Results … The results of spoligotyping analysis and a sample Spoligo pattern are presented in Table 1 and Fig. 2, respectively. Table 1 … Spoligotype family. Total isolates …  Cited by 2 Related articles All 7 versions 

 

Genetic diversity of Mycobacterium tuberculosis from Pará, Brazil, reveals a higher frequency of ancestral strains than previously reported in South America

EC Conceição, N RastogiD Couvin, ML Lopes… - Infection, Genetics and …, 2017 - Elsevier… In this database, Spoligotype International Type (SIT) designates patterns shared by two or … used the SpolSimilaritySearch tool to highlight the specificity of spoligotyping signatures in … Distribution of patient demographics, drug-susceptibility testing results and major spoligotypes …Cited by 5 Related articles All 8 versions

 

[HTML] Genetic diversity of Mycobacterium tuberculosis and transmission associated with first-line drug resistance: a first analysis in Jalisco, Mexico

G Lopez-Avalos, G Gonzalez-Palomar… - Journal of global …, 2017 - Elsevier… [19] using a Spoligotyping Diagnostic Kit with primers and controls (Ocimum Biosolutions LCC, Indianapolis, IN) following the manufacturer's instructions … In this database, spoligotype international type (SIT) designates a spoligotyping pattern shared by two or more patient …Cited by 6 Related articles All 7 versions

 

Cross-contamination of Mycobacterium tuberculosis at the National Rference Laboratory of Tuberculosis of Cuba

MR Martínez Romero, M Sardiña Aragón… - Revista Cubana de …, 2017 - medigraphic.com… A los aislamientos se les extrajo el ADN según lo descrito por van Soolingen y otros.8 Se empleóel sistema Spoligotyping Kit IM9701 (Ocimum Biosolutions Ltd, Hyderabad, India), de acuerdocon las instrucciones del fabricante y lo descrito por Kamerbeek y otros9 Los …  Related articles All 2 versions 

 

[HTML] PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping

B Molina-Moya, A Lacoma, N García-Sierra, S Blanco… - Scientific reports, 2017 - nature.com… Spoligotyping was performed using the spoligokit (Ocimum Biosolutions, Hyderabad, India) following the manufacturer's instructions … Spoligotyping International Types (SIT) were assigned according to spoligotype pattern signatures provided in SITVITWEB … Cited by 1 Related articles All 7 versions

 

Strains of Mycobacterium tuberculosis transmitting infection in Brazilian households and those associated with community transmission of tuberculosis

SA Vinhas, EC Jones-López, RR Rodrigues… - Tuberculosis, 2017 - Elsevier… frequencies of MTB isolates clustered assorted by RFLP and Spoligotyping in Vitoria … groups, the isolates were compared with those classified as Spoligotype International Type … Genotypes (IS6110 RFLP patterns and spoligotypes) were grouped according to three approaches … Cited by 2 Related articles All 8 versions

 

Genomic, Genetic, and Functional Interrogation of Mycobacterium tuberculosis Outbreak Strains

JW Saelens - 2017 - search.proquest.com… 45 2.6.3 Spoligotyping ..... 45 … xi List of Tables Table 1: Spoligotypes and patient risk factors for the 11 identified Beijing isolates from the Clínica Familiar “Luis Angel García” (CFLAG) and its associated public hospital …

 

Influência de fatores de risco do paciente e de características de Mycobacterium tuberculosis no desfecho dos casos novos de tuberculose pulmonar tratados com …

MC Scheffer - 2017 - repositorio.ufsc.br… de todos os spoligotypes encontrados nos isolados de M. tuberculosis incluídos no estudo, classificação segundo SITVIT-WEB..... 87 Figura 22- Distribuição das famílias e subfamílias segundo genotipagem por metodologia de Spoligotyping de isolados de M …  Related articles All 2 versions 

 

EPIDEMIOLOGY OF TUBERCULOSIS IN CAMELS SLAUGHTERED AT AKAKI ABATTOIR, CENTRAL ETHIOPIA

J YASMIN - 2017 - 213.55.95.56… while the rest were non-tuberculous mycobacteria (NTM). Spoligotyping of the 39 isolates indicated that majority of the isolates (94.9%) identified as orphan strains with no shared spoligotype international type (SIT) in the Spoligotype database while two …  Related articles 

 

[PDF] Characterization of efflux pumps genes involved in second-line drug resistance of tuberculosis

LA Malinga - 2017 - pdfs.semanticscholar.orgPage 1. Characterization of efflux pumps genes involved in second-line drug resistance of tuberculosis Lesibana Anthony Malinga © University of Pretoria Page 2. CHARACTERIZATION OF EFFLUX PUMPS GENES INVOLVED IN …

 

2016

 

Determination of genotypic varieties and genotyping of multiple drug-resistant tuberculosis by the RFLP and spoligotyping methods

T Karsligil, A BAYRAM, F EKŞİ - Turkish journal of medical …, 2016 - journals.tubitak.gov.tr The spoligotype patterns were encoded in triplet codes, and the types were identified (Figure 1). The obtained results were compared with the international spoligotyping database, and the spoligotypes were detected (15) …

  Related articles All 9 versions 

 

Role of gyrB mutations in pre-extensively and extensively drug-resistant tuberculosis in Thai clinical isolates

A Disratthakit, T Prammananan… - Antimicrobial agents …, 2016 - Am Soc Microbiol Cited by 10 Related articles All 7 versions

 

 

Secretome profiling of highly virulent Mycobacterium bovis 04-303 strain reveals higher abundance of virulence-associated proteins

F Vargas-Romero, G Mendoza-Hernández… - Microbial …, 2016 - Elsevier… From the M. bovis strains, 19 different spoligotyping patterns were observed among the 27 … M.bovis molecular typing database (Table 1). The most frequently observed spoligotypes were the … Only the 444 strain presented the spoligotype SB0140 and were used for comparison … Cited by 5 Related articles All 6 versions

 

[HTML] Evolutionary History and Ongoing Transmission of Phylogenetic Sublineages of Mycobacterium tuberculosis Beijing Genotype in China

Q Yin, H Liu, W Jiao, Q Li, R Han, J Tian, Z Liu… - Scientific reports, 2016 - nature.com… IS6110-RFLP or 24-VNTR) or slowly evolving phylogenetic markers (eg spoligotypes, RD and …in M. tuberculosis prevent from making any phylogenetic inference from the spoligotyping data of … For example, spoligotype SIT190 with deleted signal #40 was found in strains of both …Cited by 15 Related articles All 9 versions

 

[HTML] Genetic diversity of Mycobacterium tuberculosis complex strains isolated from patients with pulmonary tuberculosis in Anambra State, Nigeria

GN Uzoewulu, L Lawson, IS Nnanna, N Rastogi… - International journal of …, 2016 - Elsevier… The identified spoligotypes and five-loci ETR patterns were analyzed using the BioNumerics … result of the comparison of spoligotyping versus VNTRs demonstrated that spoligotyping analysis alone …with five-loci ETRs; for example, isolates with identical spoligotype pattern SIT61 …Cited by 8 Related articles All 15 versions

 

[HTML] Genetic diversity of Mycobacterium tuberculosis isolates from central India

P Desikan, DS Chauhan, P Sharma… - The Indian journal of …, 2016 - ncbi.nlm.nih.gov… Spoligotyping was carried out by using a commercial kit (Ocimum Biosolutions, Hyderabad) at …SITVIT2 database of Institute Pasteur de Guadeloupe 5 to locate families of spoligotypes … of comparison, the database consisted of a total of 7105 spoligotype patterns (corresponding …Cited by 4 Related articles All 6 versions

 

First insight into the molecular epidemiology of Mycobacterium tuberculosis in Santa Catarina,southern Brazil

CL Nogueira, RI Prim, SG Senna, DB Rovaris… - Tuberculosis, 2016 - Elsevier… Spoligotyping was performed by using commercially available membranes (Ocimum Biosolutions,Hyderabad … In this database, Spoligotype International Type (SIT) designates patterns shared by … The evolutionary relationships among all the observed spoligotypes were studied …Cited by 11 Related articles All 9 versions

 

[HTML] Analysis of Mycobacterium tuberculosis genotypic lineage distribution in Chile and neighboring countries

J Lagos, D Couvin, L Arata, J Tognarelli, C Aguayo… - PloS one, 2016 - journals.plos.org… The Fig 1B shows the spoligotyping-based MST; it is evident that LAM and T lineages … LAM9 cluster was the largest node evolved from SIT53/T1 and multiple spoligotypes were observed to be rising from it, eg, SIT33/LAM3, while the second largest spoligotype pattern SIT50/H3 … Cited by 4 Related articles All 13 versions 

 

[PDF] Molecular characterization of Mycobacterium tuberculosis complex in Gambella region, Southwest Ethiopia

G Asebe, A Worku, A Zewude, K Tafess… - African Journal of …, 2016 - researchgate.net… Figure 3. Spoligotype patterns of isolates obtained from Gambella … Page 7. 1092 Afr. J. Microbiol. Res. Table 3. Proportion of spoligotyping pattern of the strains with different variables … Table 4. Distribution of spoligotypes and their report status from Ethiopia … Cited by 4 Related articles All 3 versions 

 

[HTML] A novel molecular strategy for surveillance of multidrug resistant tuberculosis in high burden settings

HM Said, N Kushner, SV OmarAW Dreyer… - PloS one, 2016 - journals.plos.org… Genotyping. Spoligotyping was done using a commercial kit (Ocimum Biosolutions, India) according to the manufacturer's instructions [20]; spoligotypes were uploaded as binary codes in the SpolDB4 database for SIT (Spoligotype International Type) assignment …

 

[PDF] Molecular genotypes of Mycobacterium tuberculosis strains circulating in Dakar, Senegal

AB Diallo, OG Walbang, M Camara, S Lo… - African Journal of …, 2016 - researchgate.net… Spoligotyping and assignment of mycobacterial lineage and families … consecutive smear- positive TB patients out of which 15 (6.8%) did not yield a spoligotype pattern and … remaining 203 M. tuberculosis complex isolates, we identified a total of 59 different spoligotypes patterns …  Cited by 2 Related articles All 2 versions 

 

Molecular typing of mycobacteria isolated from tuberculous lymphadenitis cases in Addis Ababa, Ethiopia

O Zewdie, A Mihret, G Ameni, A Worku… - … of Tuberculosis and …, 2016 - ingentaconnect.com… Spoligotyping was used as the primary tool for typing MTC strains because of its ease of … SIT =spoligotype international type; CAS = Central Asian; EA = Euro-American; IO = Indo-Oceanic; LAM … A long descendant branch of spoligotypes belonged to the dominant 'ill-defined' T …Cited by 5 Related articles All 5 versions

 

[HTML] Whole-genome sequencing analysis of serially isolated multi-drug and extensively drug resistant Mycobacterium tuberculosis from Thai patients

K Faksri, JH Tan, A Disratthakit, E Xia… - PloS one, 2016 - journals.plos.org… Classical genotyping. Spoligotyping was performed according to the manufacturer's instructions (Ocimum Biosolutions, India) as previously described [19]. Twenty four loci MIRU-VNTR typing was performed according to the …

 

Latin-American-Mediterranean lineage of Mycobacterium tuberculosis: Human traces across pathogen's phylogeography

I Mokrousov, A Vyazovaya, T Iwamoto, Y Skiba… - Molecular phylogenetics …, 2016 - Elsevier… Spoligotyping was performed with in-house made membranes or using commercial kits (Ocimum Biosolutions … Profile #6 includes strains of 7 different spoligotypes from distant parts of Russia (Northwest … strains with SIT1321 and SIT64 that do not bear this spoligotype signature …

 

[HTML] Molecular typing of Mycobacterium Bovis from cattle reared in Midwest Brazil

RCT Carvalho, SEG Vasconcellos, M de Azevedo Issa… - PloS one, 2016 - journals.plos.org… The geographic distribution of the spoligotypes is presented in Fig 1 … bovis spoligotype found in strains isolated at municipalities of Midwest Brazil. https://doi.org/10.1371/journal.pone.0162459. g001 … by spoligotyping method. https://doi.org/10.1371/journal.pone.0162459.t001 …

 

[HTML] Chromosomal rearrangements and protein globularity changes in Mycobacterium tuberculosis isolates from cerebrospinal fluid

SH SawJL TanXY Chan, KG Chan, YF Ngeow - PeerJ, 2016 - peerj.com… The genotypes identified using the spoligotyping kit from Ocimum Biosolutions (Hyderabad, India) were Beijing ST1 (UM-CSF01, UM-CSF05, UM-CSF08, UM-CSF09 and UM-CSF17), EAI_IND (UM-CSF06), Unknown (UM-CSF04) and H3 ST50 (UM-CSF15) …  Cited by 3 Related articles All 11 versions 

 

[HTML] Contaminación cruzada por Mycobacterium tuberculosis en el Laboratorio Nacional de Referencia de Tuberculosis de Cuba

MR Martínez Romero, M Sardiña Aragón… - Revista Cubana de …, 2016 - scielo.sld.cu… 8 Se empleó el sistema Spoligotyping Kit IM9701 (Ocimum Biosolutions Ltd, Hyderabad, India), de acuerdo con las instrucciones del fabricante y lo descrito por Kamerbeek y otros 9 Los patrones obtenidos se compararon con la base de datos internacional SITVIT2 "en línea" …  Cited by 1 Related articles All 6 versions 

 

Mycobacterium Tuberculosis In Central Ethiopia: Molecular Epidemiology, Drug Sensitivity Patterns, and Evaluation of the Genotype Mtbdrplus Assay for the Detection …

Z Bedewi - 2016 - 213.55.95.56… The isolates were analyzed using region of difference (RD) 9-based polymerase chain reaction (PCR) and spoligotyping … Of these 90 spoligotypes, 32 were clustered consisting of 223 (79.3%) isolates while 58 had only a single isolate each …

  

The epidemiology of tuberculosis in cattle and humans living in the wildlife-livestock-human interface in the rural Mnisi community Mpumalanga province South Africa

J Musoke - 2016 - repository.up.ac.za… 85 5.3.5.2 Polymerase Chain Reaction (PCR) .... 86 5.3.5.3 Spoligotyping ..... 86 5.3.5.4 Mycobacterial interspersed repetitive-unit-variable number tandem ..... 87 …

 

[HTML] Molecular analysis of genetic mutations among cross-resistant second-line injectable drugs reveals a new resistant mutation in Mycobacterium tuberculosis

L Malinga, J Brand, S Olorunju, A Stoltz… - … and infectious disease, 2016 - Elsevier… Spoligotyping. The PCR products were amplified using primers purchased from manufacturer (Ocimum Biosciences, India) and the procedure was performed as … Amersham Biosciences) and by exposure to X-ray film (HyperfilmECL, Amersham).The spoligotypes were reported …

Association of Mycobacterium tuberculosis genotypes and treatment outcome in pulmonary tuberculosis patients in Tshwane metropolitan area

SR Matukane - 2016 - repository.up.ac.za

… 34 3.3.2 Data collection 35 3.3.3 DNA extraction 35 3.3.4 Spoligotyping 35 3.3.5 MIRU-VNTR typing 36 Page 8 … 38 4.1 Results 38 4.1.1 Spoligotyping and 24-loci MIRU-VNTR typing results 38 4.1.2 Association of genotypes and drug resistance with treatment outcome 39 …  Related articles All 3 versions 

 

Wild-type minimal inhibitory concentration distributions of secondline drugs in mycobacterium tubercolosis complex clinical isolated in relation to recommended critical …

NM Seloma - 2016 - ulspace.ul.ac.za… SpolDB4 - is an international spoligotyping database that defines M. tuberculosis complex genetic polymorphism, isolates sharing identical spoligotype pattern are defined according to SIT Spoligotype International Type) isolates with single spoligotype patterns are defined as …  Related articles All 2 versions 

 

Development and validation of a molecular assay and evaluation of the GeneXpert® MTB/RIF assay for the rapid detection of genital tuberculosis

MC Sokhela - 2016 - scholar.ufs.ac.za… Figure 3.1 Prevalence of spoligotypes in South Africa ..... 56 Figure 3.2 MIRU-VNTR 24 loci copy number ..... 62Figure 3.3 MIRU-VNTR and Spoligotyping results …

 

[HTML] Tuberculosis por Mycobacterium bovis en trabajadores de fincas en saneamiento para tuberculosis bovina, de Antioquia, Boyacá y Cundinamarca

AF Leal-BohórquezCM Castro-Osorio… - Revista de Salud …, 2016 - SciELO Public Health… Kremer K, Bunschoten A, Schouls L, Van Soolingen D, Van Embden J. Laboratory Spoligotyping, a PCR-based method to simultaneously detect … el producto de la reacción en cadena de la polimerasa (RCP) se hibridó en una membrana comercial (Ocimum Biosolutions India …

 

[PDF] Usefulness of molecular methods for detecting drug resistance and genotyping in mycobacterium tuberculosis

BM Moya - 2016 - core.ac.uk… RFLP) .... 43 1.7.2. Spoligotyping ..... 44 … RFLP) .... 64 4.4.2. Spoligotyping ..... 65 …  Related articles All 3 versions

 

Usefulness of molecular methods for detecting drug resistance and genotyping in mycobacterium tuberculosis

B Molina Moya - 2016 - ddd.uab.cat… RFLP) .... 43 1.7.2. Spoligotyping ..... 44 … RFLP) .... 64 4.4.2. Spoligotyping ..... 65 …  Related articles 

 

 

[HTML] Tuberculosis caused by Mycobacterium bovis in workers of bovine tuberculosis sanitation farms in Antioquia, Boyacá and Cundinamarca

AF Leal-Bohórquez, CM Castro-Osorio… - Revista de Salud …, 2016 - scielo.org.co… La metodología de Spoligotyping se realizó según lo descrito por Kremer en el 2002 … por 30 segundos y una extensión final a 72°C por 5 minutos; el producto de la reacción en cadena de la polimerasa (RCP) se hibridó en una membrana comercial (Ocimum Biosolutions India … Related articles All 3 versions 

 

[BOOK] Clinical and pathological characteristics of the Uganda genotype of Mycobacterium tuberculosis

D Wamala - 2016 - openarchive.ki.se… A study of the evolutionary relationships and worldwide distribution of the spoligotypes of Mtb isolates from Ugandan patients with tuberculous lymphadenitis indicated an ongoing evolution of the Uganda genotype, with Uganda at the center of this evolution (Paper II) …  Related articles All 3 versions 

 

Genotipificación de Mycobacterium tuberculosis en aislados clínicos obtenidos de pacientes VIH positivos de los hospitales Simón Bolívar y Santa Clara de Bogotá

MY Beltrán León - 2016 - bdigital.unal.edu.co… A total of 49 isolates of Mycobacterium tuberculosis were genotyped by spoligotyping and MIRU VNTR loci 24 … Spoligotype analysis showed 15 patterns, of which 3/49 (6,1%) were orphans and 45/49 (91,8%) belonged to the Euro American lineage …  Cited by 4 Related articles 

 

Genotipos de Mycobacterium tuberculosis prevalentes en Argentina y su relación con mutaciones de resistencia a isoniacida

J Monteserin - 2016 - ridaa.unq.edu.ar

… La genotipificación se realizó mediante spoligotyping, y en casos selectos, por MIRU-VNTR24. El análisis de clustering se realizó mediante RFLP IS6110 … como spoligotyping y MIRUs-VNTR (Mycobacterial interspersed repetitive units-variable …

Molecular typing of mycobacteria isolated from tuberculous lymphadenitis cases in Addis Ababa, Ethiopia.O. Zewdie, A. Mihret, G. Ameni, A. Worku, T. Gemechu, T. Abebe,Int J Tuberc Lung Dis.Author manuscript; available in PMC 2017 Nov 1.Published in final edited form as: Int J Tuberc Lung.Dis. 2016 Nov; 20(11): 1529–1534.  doi: 10.5588/ijtld.15.1023.PMCID: PMC5665165

 Evolutionary History and Ongoing Transmission of Phylogenetic Sublineages of Mycobacterium tuberculosis Beijing Genotype in China,Qing-qin Yin, Hai-can Liu, Wei-wei Jiao, Qin-jing Li, Rui Han, Jian-ling Tian, Zhi-guang Liu, Xiu-qin Zhao, Ying-jia Li, Kang-lin Wan, A-dong Shen, IgorMokrousov,Sci Rep. 2016; 6: 34353.  Published online 2016 Sep 29. doi: 10.1038/srep34353.PMCID: PMC5041183

Chromosomal rearrangements and protein globularity changes in Mycobacterium tuberculosis isolates from cerebrospinal fluid.Seow Hoon Saw, Joon Liang Tan, Xin Yue Chan, Kok Gan Chan, Yun Fong Ngeow.PeerJ. 2016; 4: e2484.  Published online 2016 Sep 21. doi: 10.7717/peerj.2484.PMCID: PMC5036109

Molecular Typing of Mycobacterium bovis from Cattle Reared in Midwest Brazil,Ricardo César Tavares Carvalho, Sidra Ezidio Gonçalves Vasconcellos, Marina de Azevedo Issa, Paulo Martins Soares Filho, Pedro Moacyr Pinto Coelho Mota, Flábio Ribeiro de Araújo, Ana Carolina da Silva Carvalho, Harrison Magdinier Gomes, Philip Noel Suffys, Eduardo Eustáquio de Souza Figueiredo, Vânia Margaret.Flosi Paschoalin.PLoS One. 2016; 11(9): e0162459.  Published online 2016 Sep 15. doi: 10.1371/journal.pone.0162459. PMCID: PMC5024986

Role of gyrB Mutations in Pre-extensively and Extensively Drug-Resistant Tuberculosis in Thai  Clinical Isolates.Areeya Disratthakit, Therdsak Prammananan, Chanwit Tribuddharat, Iyarit  Thaipisuttikul, Norio Doi, Manoon Leechawengwongs, Angkana Chaiprasert,Antimicrob Agents Chemother. 2016 Sep; 60(9): 5189–5197.  Prepublished online 2016 Jun 13. Published online 2016 Aug 22. doi: 10.1128/AAC.00539-16.PMCID: PMC4997844

Analysis of Mycobacterium tuberculosis Genotypic Lineage Distribution in Chile and Neighboring Countries.Jaime Lagos, David Couvin, Loredana Arata, Javier Tognarelli, Carolina Aguayo, TamaraLeiva, Fabiola Arias, Juan Carlos Hormazabal, Nalin Rastogi, Jorge Fernández,PLoS One. 2016; 11(8): e0160434.  Published online 2016 Aug 12. doi: 10.1371/journal.pone.0160434.PMCID: PMC4982630

Whole-Genome Sequencing Analysis of Serially Isolated Multi-Drug and Extensively Drug Resistant Mycobacterium tuberculosis from Thai Patients.Kiatichai Faksri, Jun Hao Tan, Areeya Disratthakit, Eryu Xia, Therdsak Prammananan, Prapat Suriyaphol, Chiea Chuen Khor, Yik-Ying Teo, Rick Twee-Hee Ong, Angkana Chaiprasert.PLoS One. 2016; 11(8): e0160992.  Published online 2016 Aug 12. doi: 10.1371/journal.pone.0160992.PMCID: PMC4982626

Genetic diversity of Mycobacterium tuberculosis isolates from central India.Prabha Desikan, D.S. Chauhan, Pragya Sharma, Nikita Panwalkar, Manju Chourey, Mohan Lal Patidar, Priyanka Yadav, V. Chandrasekaran, B.S. Ohri.Indian J Med Res. 2016 Apr; 143(4): 481–486.doi: 10.4103/0971-5916.184287.PMCID: PMC4928555

Association between human leukocyte antigen class II and pulmonary tuberculosis due to mycobacterium tuberculosis in Uganda.Dan Wamala, Helen Koyokoyo Buteme, Samuel Kirimunda, Gunilla Kallenius, Moses Joloba.BMC Infect Dis. 2016; 16: 23.  Published online 2016 Jan 23.doi: 10.1186/s12879-016-1346-0.PMCID: PMC4724396

A Novel Molecular Strategy for Surveillance of Multidrug Resistant Tuberculosis in High BurdenettingsHalima M. Said, Nicole Kushner, Shaheed V. Omar, Andries W. Dreyer, Hendrik Koornhof, Linda Erasmus,Yasmin Gardee, Ivy Rukasha, Elena Shashkina, Natalie Beylis, Gilla Kaplan, Dorothy Fallows,Nazir A. Ismail.PLoS One. 2016; 11(1): e0146106. Published online 2016 Jan 11.doi: 10.1371/journal.pone.0146106.PMCID: PMC4713439

 

2015

Genetic Diversity of Mycobacterium tuberculosis Isolates from Assam, India: Dominance of Beijing Family and Discovery of Two New Clades Related to CAS1_Delhi and EAI Family Based on Spoligotyping and MIRU-VNTR Typing.Kangjam Rekha Devi, Rinchenla Bhutia, Shovonlal Bhowmick, Kaustab Mukherjee, Jagadish Mahanta,Kanwar Narain.PLoS One. 2015; 10(12): e0145860.  Published online 2015.Dec 23. doi: 10.1371/journal.pone.0145860.PMCID: PMC4689458

Multidrug-Resistant Mycobacterium tuberculosis of the Latin American Mediterranean Lineage, Wrongly Identified as Mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), Causing Active Tuberculosis in South Brazil,Elis R. Dalla Costa, Sidra E. G. Vasconcelos, Leonardo S. Esteves, Harrison M. Gomes, Lia L. Gomes, Pedro Almeida da Silva, João Perdigão, Isabel Portugal, Miguel Viveiros, Ruth McNerney, Arnab Pain, Taane G. Clark, Nalin Rastogi, Gisela Unis, Maria Lucia R. Rossetti, Philip Noel Suffys,J Clin Microbiol. 2015 Dec; 53(12): 3805–3811.  Prepublished online.2015 Sep 23. Published online 2015 Nov 18. doi: 10.1128/JCM.02012-15.PMCID: PMC4652117

 Genetic Diversity of Mycobacterium tuberculosis Complex Isolated from Tuberculosis Patients in Bahir Dar City and Its Surroundings, Northwest Ethiopia.Anwar Nuru, Gezahegne Mamo, Adane Worku, Aschalew Admasu, Girmay Medhin, Rembert Pieper, Gobena Ameni.Biomed Res Int. 2015; 2015: 174732.Published online 2015 Sep 28. doi: 10.1155/2015/174732.PMCID: PMC4600926

 Predominance of Uganda genotype of Mycobacterium tuberculosis isolated from Ugandan patients with tuberculous lymphadenitis.Dan Wamala, Moses Okee, Edgar Kigozi, David Couvin, Nalin Rastogi, Moses Joloba, Gunilla Kallenius,BMC Res Notes. 2015; 8: 398.  Published online 2015 Sep 1. doi: 10.1186/s13104-015-1362-y.PMCID: PMC4556223

Molecular Typing of Mycobacterium Tuberculosis Complex by 24-Locus Based MIRU-VNTR Typing inConjunction with Spoligotyping to Assess Genetic Diversity of Strains Circulating in Morocco .Nada Bouklata, Philip Supply, Sanae Jaouhari, Reda Charof, Fouad Seghrouchni, Khalid Sadki, Youness El Achhab, Chakib Nejjari, Abdelkarim Filali-Maltouf, Ouafae Lahlou, Rajae El Aouad,PLoS One. 2015; 10(8): e0135695.  Published online 2015 Aug 18. doi: 10.1371/journal.pone.0135695.PMCID: PMC4540494

Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.Rodrigo Ivan Prim, Marcos André Schörner, Simone Gonçalves Senna, Christiane Lourenço Nogueira, Anna,Carolina Cançado Figueiredo, Jaquelline Germano de Oliveira, Darcita Bürger Rovaris, Maria Luiza Bazzo.Mem Inst Oswaldo Cruz. 2015 Aug; 110(5): 618–623.  doi: 10.1590/0074-02760150100.PMCID: PMC4569824

Rapid Whole-Genome Sequencing of Mycobacterium tuberculosis Isolates Directly from Clinical Samples.Amanda C. Brown, Josephine M. Bryant, Katja Einer-Jensen, Jolyon Holdstock, Darren T Houniet, Jacqueline Z. M. Chan, Daniel P. Depledge, Vladyslav Nikolayevskyy, Agnieszka Broda, Madeline J. Stone,  Mette T. Christiansen, Rachel Williams, Michael B. McAndrew, Helena Tutill, Julianne Brown, Mark Melzer, Caryn Rosmarin, Timothy D. McHugh, Robert J. Shorten, Francis Drobniewski, Graham Speight, Judith Breuer.J Clin Microbiol. 2015 Jul; 53(7): 2230–2237.  Prepublished online 2015 May 13. Published online. 2015 Jun 18. doi: 10.1128/JCM.00486-15.PMCID: PMC4473240

 

 Comparative Whole-Genome Analysis of Clinical Isolates Reveals Characteristic Architecture of Mycobacterium tuberculosis Pangenome.Vinita Periwal, Ashok Patowary, Shamsudheen Karuthedath Vellarikkal, Anju Gupta, Meghna Singh,Ashish Mittal, Shamini Jeyapaul, Rajendra Kumar Chauhan, Ajay Vir Singh, Pravin Kumar Singh, Parul,Garg, Viswa Mohan Katoch, Kiran Katoch, Devendra Singh Chauhan, Sridhar Sivasubbu, Vinod Scaria.PLoS One. 2015; 10(4): e0122979.  Published online 2015 Apr 8. doi: 10.1371/journal.pone.0122979.PMCID: PMC4390332

A First Insight on the Population Structure of Mycobacterium tuberculosis Complex as Studied by Spoligotyping and MIRU-VNTRs in Santiago, Chile.María Elvira Balcells, Patricia García, Paulina Meza, Carlos Peña, Marcela Cifuentes, David Couvin,PLoS One. 2015; 10(2): e0118007.  Published online 2015 Feb 11. doi: 10.1371/journal.pone.0118007.PMCID: PMC4324903

Multidrug-Resistant Tuberculosis Outbreak in Gaming Centers, Singapore, 2012.Cynthia B.E. Chee, Suay-Hong Gan, Rick T. Ong, Li-Hwei Sng, Christopher W. Wong, Jeffery Cutter, Min.Gong, Hui-Maan Seah, Li Yang Hsu, Suhana Solhan, Peng-Lim Ooi, Eryu Xia, Jayne T. Lim, Chwee-Kim .Koh, Soon-Kok Lim, Han-Kee Lim, Yee-Tang Wang.Emerg Infect Dis. 2015 Jan; 21(1): 179–180.  doi: 10.3201/eid2101.141159.PMCID: PMC4285258

 

2014

 

Study of Mycobacterium tuberculosis Complex Genotypic Diversity in Malaysia Reveals a Predominance of Ancestral East-African-Indian Lineage with a Malaysia-Specific SignatureFazli Ismail, David Couvin, Izzah Farakhin, Zaidah Abdul Rahman, Nalin Rastogi, Siti SuraiyaPLoS One. 2014; 9(12): e114832.  Published online 2014 Dec 11. doi: 10.1371/journal.pone.0114832.PMCID: PMC4263714

Diversity of Mycobacterium tuberculosis and drug resistance in different provinces of Papua New Guinea.Serej D Ley, Paul Harino, Kilagi Vanuga, Ruben Kamus, Robyn Carter, Christopher Coulter,  Sushil.Pandey, Julia Feldmann, Marie Ballif, Peter M Siba, Suparat Phuanukoonnon, Sebastien Gagneux,Hans-Peter Beck.BMC Microbiol. 2014; 14: 307.  Published online 2014 Dec 5. doi: 10.1186/s12866-014-0307-2.PMCID: PMC4264550

Molecular Confirmation of Bacillus Calmette Guerin Vaccine Related Adverse Events among Saudi Arabian Children.Sahal Al-Hajoj, Ziad Memish, Naila Abuljadayel, Raafat AlHakeem, Fahad AlRabiah, Bright Arghese,PLoS One. 2014; 9(11): e113472.  Published online 2014 Nov 9 doi: 10.1371/journal.pone.0113472.PMCID: PMC4237431

Tuberculosis recurrence in a high incidence setting for HIV and tuberculosis in Brazil. Gisela Unis, Andrezza Wolowski Ribeiro, Leonardo Souza Esteves, Fernanda Sá Spies, Pedro Dornelles Picon, Elis Regina Dalla Costa, Maria Lucia Rosa Rossetti,BMC Infect Dis. 2014; 14: 548.  Published online 2014 Oct 24. doi: 10.1186/s12879-014-0548-6.PMCID: PMC4215011

 Strain Classification of Mycobacterium tuberculosis Isolates in Brazil Based on Genotypes Obtained by Spoligotyping, Mycobacterial Interspersed Repetitive Unit Typing and the Presence of Large Sequence and Single Nucleotide Polymorphism.Sidra E. G. Vasconcellos, Chyntia Carolina Acosta, Lia Lima Gomes, Emilyn Costa Conceição, KarlaValéria Lima, Marcelo Ivens de Araujo, Maria de Lourdes Leite, Flávio Tannure, Paulo Cesar de Souza Caldas, Harrison M. Gomes, Adalberto Rezende Santos, Michel K. Gomgnimbou, Christophe Sola, David Couvin, Nalin Rastogi, Neio Boechat, Philip Noel SuffysPLoS One. 2014; 9(10): e107747.  Published online 2014 Oct 14. doi: 10.1371/journal.pone.0107747.PMCID: PMC4196770

Prevalence of Extensively Drug Resistant Tuberculosis among Archived Multidrug Resistant Tuberculosis Isolates in Zimbabwe.Tichaona Sagonda, Lucy Mupfumi, Rumbidzai Manzou, Beauty Makamure, Mqondisi Tshabalala, Lovemore Gwanzura, Peter Mason, Reggie Mutetwa.Tuberc Res Treat. 2014; 2014: 349141.  Published online 2014 May 20. doi: 10.1155/2014/349141.PMCID: PMC4054961

Clinico-pathological features of tuberculosis due to Mycobacterium tuberculosis Uganda genotype in patients with tuberculous lymphadenitis: a cross sectional study. Dan Wamala, Benon Asiimwe, Edgar Kigozi, Gerald Mboowa, Moses Joloba, Gunilla Kallenius.BMC Clin Pathol. 2014; 14: 14.  Published online 2014 Apr 2. doi: 10.1186/1472-6890-14-14.PMCID: PMC3974774

Strain Diversity of Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Afar Pastoral Region of Ethiopia.Mulugeta Belay, Gobena Ameni, Gunnar Bjune, David Couvin, Nalin Rastogi, Fekadu Abebe.Biomed Res Int. 2014; 2014: 238532.  Published online 2014 Mar 6. doi: 10.1155/2014/238532.PMCID: PMC3966356

 

2013

 

Molecular Epidemiology of Mycobacterium tuberculosis Complex in Singapore, 2006-2012.Leo Kang-Yang Lim, Li Hwei Sng, Wah Win, Cynthia Bin-Eng Chee, Li Yang Hsu, Estelle Mak, Arul Earnest, Marcus Eng-Hock Ong, Jeffery Cutter, Yee Tang Wang.PLoS One. 2013; 8(12): e84487.  Published online 2013 Dec 18. doi: 10.1371/journal.pone.0084487.PMCID: PMC3867517

 Global Study of IS6110 in a Successful Mycobacterium tuberculosis Strain: Clues for Deciphering Its Behavior and for Its Rapid Detection.Maria Isabel Millán-Lou, Ana Isabel López-Calleja, Cristina Colmenarejo, Maria Antonia Lezcano, Maria Asunción Vitoria, Patricia del Portillo, Isabel Otal, Carlos Martín, Sofía Samper. J Clin Microbiol. 2013 Nov; 51(11): 3631–3637.  doi: 10.1128/JCM.00970-13.PMCID: PMC3889744

Tuberculosis Transmission among Immigrants and Autochthonous Populations of the Eastern Province of Saudi Arabia.Bright Varghese, Philip Supply, Mohammed Shoukri, Caroline Allix-Beguec, Ziad Memish, Naila Abuljadayel, Raafat Al-Hakeem, Fahad AlRabiah, Sahal Al-Hajoj.PLoS One. 2013; 8(10): e77635.  Published online 2013 Oct 17. doi: 10.1371/journal.pone.0077635.PMCID: PMC379832

 Efficacy and safety of thrice weekly DOTS in tuberculosis patients with and without HIV co-infection: an observational study.Richa Vashishtha, Krishna Mohan, Bhagteshwar Singh, Satish K Devarapu, Vishnubhatla Sreenivas, Sanjay Ranjan, Deepak Gupta, Sanjeev Sinha, Surendra K Sharma.BMC Infect Dis. 2013; 13: 468.  Published online 2013 Oct 7. doi: 10.1186/1471-2334-13-468.PMCID: PMC3852441

 Extrapulmonary Tuberculosis: Mycobacterium tuberculosis Strains and Host Risk Factors in a Large Urban Setting in Brazil.Teresa Gomes, Solange Alves Vinhas, Bárbara Reis-Santos, Moisés Palaci, Renata Lyrio Peres, Paola P. Aguiar, Fabiola Karla Correa Ribeiro, Hebert Silva Marques, Valdério do Valle Dettoni, John L.

 Johnson, Lee W. Riley, Ethel Leonor Maciel PLoS One. 2013; 8(10): e74517.  Published online 2013 Oct 2. doi: 10.1371/journal.pone.0074517.PMCID: PMC3788772

Immunogenic Mycobacterium africanum Strains Associated with Ongoing Transmission in The Gambia

Florian Gehre, Martin Antonio, Jacob K. Otu, Neneh Sallah, Oumie Secka, Tutty Faal, Patrick Owiafe, Jayne S. Sutherland, Ifedayo M. Adetifa, Martin O. Ota, Beate Kampmann, Tumani Corrah, Bouke C. de Jong .Emerg Infect Dis. 2013 Oct; 19(10): 1593–1604.  doi: 10.3201/eid1910.121023.PMCID: PMC3810728

Mycobacterium tuberculosis is the causative agent of tuberculosis in the southern ecological zones of Cameroon, as shown by genetic analysis.Jean Paul Assam Assam, Véronique Penlap Beng, Fidelis Cho-Ngwa, Michel Toukam, Ane-Anyangwe Irene Ngoh, Mercy Kitavi, Inoster Nzuki, Juliette N Nyonka, Emilienne Tata, Jean Claude Tedom, Robert A Skilton, Roger Pelle, Vincent P K Titanji BMC Infect Dis. 2013; 13: 431.  Published online 2013 Sep 13. doi: 10.1186/1471-2334-13-431.PMCID: PMC3851

 Primary Drug-Resistant Tuberculosis in Hanoi, Viet Nam: Present Status and Risk Factors Nguyen Thi Le Hang, Shinji Maeda, Luu Thi Lien, Pham Huu Thuong, Nguyen Van Hung, Tran Bich Thuy,  Akiko Nanri, Tetsuya Mizoue, Nguyen Phuong Hoang, Vu Cao Cuong, Khieu Thi Thuy Ngoc, Shinsaku Sakurada, Hiroyoshi Endo, Naoto Keicho.PLoS One. 2013; 8(8): e71867.  Published online 2013 Aug 13. doi: 10.1371/journal.pone.0071867.PMCID: PMC374246

MDR TB Transmission, Singapore Cynthia B.E. Chee, Li-Yang Hsu, Li-Hwei Sng, Yee-Sin Leo, Jeffery Cutter, Yee-Tang Wang .Emerg Infect Dis. 2013 Jul; 19(7): 1151–1152.  doi: 10.3201/eid1907.120372. PMCID: PMC3713962

Prevalence of tuberculosis in pigs slaughtered at two abattoirs in Ethiopia and molecular characterization of Mycobacterium tuberculosis isolated from tuberculous-like lesions in pigs.  Sintayehu Mulugeta Arega, Franz Josef Conraths, Gobena Ameni.BMC Vet Res. 2013; 9: 97.  Published online 2013 May 6. doi: 10.1186/1746-6148-9-97.PMCID: PMC3661388

 Abattoir-based study on the epidemiology of caprine tuberculosis in Ethiopia using conventional and molecular tools. Benti Deresa, Franz J Conraths, Gobena Ameni, Acta Vet Scand. 2013; 55(1): 15.  Published online 2013 Feb 21. doi: 10.1186/1751-0147-55-15.PMCID: PMC3639213

Admixed Phylogenetic Distribution of Drug Resistant Mycobacterium tuberculosis in Saudi Arabia. Bright Varghese, Philip Supply, Caroline Allix-Béguec, Mohammed Shoukri, Ruba Al-Omari, Mais  Herbawi, Sahal Al-Hajoj.PLoS One. 2013; 8(2): e55598.  Published online 2013 Feb 1. doi: 10.1371/journal.pone.0055598

PMCID: PMC3562184

Mycobacterium tuberculosis DNA fingerprint clusters and its relationship with RDRio genotype in Brazil

Solange Alves Vinhas, Moisés Palaci, Hebert Silva Marques, Paola Poloni Lobo de Aguiar, Fabíola Karla Ribeiro, Renata Lyrio Peres, Reynaldo Dietze, Harrison Magdinier Gomes, Philip Noel Suffys, Jonathan E. Golub, Lee W. Riley, Ethel Leonor Noia Maciel,Tuberculosis (Edinb) Author manuscript; available in PMC 2014 Mar 1. Published in final edited form as: Tuberculosis (Edinb). 2013 Mar; 93(2): 207–212. Published online 2012 Dec 8.  doi: 10.1016/j.tube.2012.09.001.PMCID: PMC3734852

Spoligotyping of multidrug-resistant Mycobacterium tuberculosis isolates in Ethiopia

B Diriba, T Berkessa, G Mamo, Y Tedla… - … International Journal of …, 2013 - ingentaconnect.com ... al.10 and as per the spoligotype kit supplier's instructions (Ocimum Biosolutions, Ijssel- stein … clinical isolates were represented by a unique (non-clustered) spoligotype pattern ... these patterns,which are already recorded in the fourth international spoligotyping database (SpolDB4 ...

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[PDF] from ajol.info

Modern lineages of Mycobacterium tuberculosis in Addis Ababa, Ethiopia: implications for the tuberculosis control programe

A Mihret, Y Bekele, M Aytenew, M Abebe, L Wassie… - African health …, 2013 - ajol.info... was carried out using the commercially available kit from Ocimum Biosolutions, India, using ... results with already existing designations in the international spoligotyping database, SpolDB4 ... 5. In this database, two or more patient isolates sharing identical spoligotype patterns are ...Related articles All 7 versions Cite

 

[HTML] from plos.org

[HTML] Admixed Phylogenetic Distribution of Drug Resistant Mycobacterium tuberculosis in Saudi Arabia

B Varghese, P Supply, C Allix-Béguec, M Shoukri… - PloS one, 2013 - dx.plos.org... grouping of the isolates with a typical Beijing spoligotype) (Figure S1). ... were identified based on isolates sharing identical MIRU-VNTR types and spoligotypes. ... loci based MIRU-VNTR typing (Genoscreen, Lille, France) and Spoligotyping (Ocimum Biosolutions, Hyderabad, India ...Related articles All 6 versions Cite More

 

[PDF] Abattoir-based study on the epidemiology of caprine tuberculosis in Ethiopia using conventional and molecular tools

B Deresa, FJ Conraths, G Ameni… - Acta Veterinaria …, 2013 - actavetscand.com ... Spoligotyping Spoligotyping was performed at ALIPB; following the procedure described earlier [28] and according to the spoligotype kit supplier's instructions (Ocimum Biosolutions Company, Iisselstein, The Netherlands). ...Related articles All 3 versions Cite

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Molecular typing of mycobacteria isolated from extrapulmonary tuberculosis patients at Debre Birhan Referral Hospital, central Ethiopia

L Garedew, A Mihret, T Abebe… - … Journal of Infectious …, 2013 - informahealthcare.com... The 34 isolates confirmed to be M. tuberculosis by deletion typing were further characterized by spoligotyping techniques, as previously described [11,12], and in accordance with the spoligotype kit supplier instructions (Ocimum Biosolutions Company, IJsselstein ...Related articles All 2 versions Cite

Endogenous reactivation followed by exogenous re-infection with drug resistant strains, a new challenge for tuberculosis control in Saudi Arabia

B Varghese, R al-Omari, C Grimshaw, S Al-Hajoj - Tuberculosis, 2013 - Elsevier... All of the available isolates from patients were subjected to spoligotyping (Ocimum Biosolutions, Hyderabad, India) and 24 loci based MIRU-VNTR typing (Genoscreen, Lille, France) by using the commercially available kits and methods described previously. ...Related articles All 4 versions Cite

 

2012

Rapid spoligotyping of Mycobacterium tuberculosis complex bacteria using a microarray system with automatic data processing and assignment

A Ruettger, J Nieter, A Skrypnyk… - Journal of Clinical …, 2012 - Am Soc Microbiol... Short-Form Paper 4 reverse line blot hybridization using the Spoligotyping Kit (Ocimum Biosolutions, Hydera- 79 bad, India) and the present DNA microarray. ... ArrayStrip spoligotyping and the assay's capability of identifying new spoligotypes and li- 109 neages. ...Import into BibTeX

 

Rapid Test for Identification of a Highly Transmissible Mycobacterium tuberculosis Beijing Strain of Sub-Saharan Origin

MI Millán-Lou, H Alonso, P Gavín… - Journal of Clinical …, 2012 - Am Soc Microbio... First, all 292 isolates were screened by spoligotyping with a commercially available kit (Ocimum Biosolutions Ltd., Hyderabad, India) by the method of Kamerbeek et al.(14). Seventy of the 292 isolates belonged to the Beijing family. ...Related articles - All 3 versions - Import into BibTeX

 

GenoType MTBDRsl performance on clinical samples with diverse genetic background

P Miotto, AM Cabibbe, P Mantegani… - European …, 2012 - Eur Respiratory Soc ... Spoligotyping. The amplified direct repeat locus of clinical isolates was investigated using the Spoligotyping kit (Ocimum Biosolutions, Hyderabad, India) following the manufacturer'sinstructions. Spoligotyping results were entered, in binary format, in the MIRU-VNTRplus web ...Related articles - All 2 versions - Import into BibTeX

 

New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Mycobacterium tuberculosis</i> strains from Saudi Arabia

B Varghese, A Hillemann, DR Wijayanti… - Infection, Genetics and …, 2012 - Elsevier... Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typingand spoligotyping. Spoligotyping was performed as previously described (Kamerbeek et al., 1997) with the commercial kit (Ocimum Biosolutions, Hyderabad, India). ...All 2 versions - Import into BibTeX

 

[HTML] DISTRIBUTION OF SPOLIGOTYPING DEFINED GENOTYPIC LINEAGES AMONG DRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS COMPLEX CLINICAL ISOLATES IN ANKARA, TURKEY

O Kisa, G Tarhan, S Gunal, A Albay, R Durmaz… - PloS one, 2012 - dx.plos.org... Spoligotyping was carried out with a commercial kit (Isogen Bioscience BV, Maarssen, The Netherlands ... tuberculosis H37Rv and M.bovis BCG P3 were used as control for spoligotyping.Spoligotypes results were converted into octal code and in the SITVIT2 proprietary database ...Related articles - Cached - All 4 versions - Import into BibTeX

 

[HTML] SPOLIGOTYPING AND DRUG RESISTANCE ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS FROM NATIONAL SURVEY IN CHINA

Y Pang, Y Zhou, B Zhao, G Liu, G Jiang, H Xia… - PloS one, 2012 - dx.plos.org... A commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as described by ... of the direct repeat region of Mycobacterium tuberculosis: implications for interpretation of spoligotyping data. ... 2004) Predominant tuberculosis spoligotypes, Delhi, India. ...Cached - Import into BibTeX

[HTML] SPOLIGOTYPING AND VARIABLE NUMBER TANDEM REPEAT ANALYSIS OF MYCOBACTERIUM BOVIS ISOLATES FROM CATTLE IN BRAZIL

PM Parreiras, GI Andrade… - Memórias do Instituto …, 2012 - SciELO Brasil... The PCR products were hybridised to a membrane (Isogen, The Netherlands) containing 43 oligonucleotides of known spacer sequences by reversed-line blot hybridisation. ... Spoligotyping - Complete spoligotypes were obtained from 61 isolates (Table I). In total, 17 ...Related articles - Cached - All 5 versions - Import into BibTeX

 

EVALUATION OF 24-LOCUS MIRU-VNTR IN EXTRAPULMONARY SPECIMENS: STUDY FROM A TERTIARY CENTRE IN MUMBAI

V Vadwai, A Shetty, P Supply… - Tuberculosis, 2012 - Elsevier... Spoligotyping, was performed using a commercial kit (Isogen, Netherlands) as per the ... 13/69,18.8%) spoligo-prototypes, compared to LAM, Haarlem or T spoligotypes represented at ... Analysis of the congruence between MIRU-VNTR and spoligotyping results combined with the …Cited by 1 - All 3 versions - Import into BibTeX

EPIDEMIOLOGICAL SIGNIFICANCE OF THE DOMESTIC BLACK PIG (SUS SCROFA) IN THE MAINTENANCE OF BOVINE TUBERCULOSIS IN SICILY

V Di Marco, P Mazzone, MT Capucchio… - Journal of Clinical …, 2012 - Am Soc Microbiol... direct repeat locus were detected by hybridization onto a spoligotyping membrane (Isogen 231 ...M. bovis isolates from both field studies were genotyped by both spoligotyping 342 ... Fourspoligotypes were identified: SB0120 (23 isolates), SB0841 (five isolates), SB0833 (two 344 ...Related articles - All 2 versions - Import into BibTeX

TRANSMISSION OF TUBERCULOSIS WITHIN FAMILY-HOUSEHOLDS

E Augustynowicz-Kopeć, T Jagielski, M Kozińska… - Journal of Infection, 2012 - Elsevier... Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen ...had different M. tuberculosis isolates, as evidenced by both spoligotyping and MIRU ... Assumingisolates revealing both identical spoligotypes and VNTR profiles are representing the ...All 2 versions - Import into BibTeX

[PDF] PREVALENCE OF PULMONARY TB AND SPOLIGOTYPE PATTERN OF MYCOBACTERIUM TUBERCULOSIS AMONG TB SUSPECTS IN A RURAL COMMUNITY IN SOUTHWEST ETHIOPIA

A Deribew, G Abebe, L Apers… - BMC Infectious …, 2012 - biomedcentral.com... Standard spoligotyping method [12] was done generally as described by Kamerbeek and colleagues using a commercially available kit (Isogen Bioscience BV ... Spoligotypes described only once (non-clustered) in this study and in the SpolDB4 were designated as ...View as HTML - Import into BibTeX

CHARACTERIZING< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES FROM KARACHI, PAKISTAN: DRUG RESISTANCE AND GENOTYPES

A Ayaz, Z Hasan, S Jafri, R Inayat, R Mangi… - International Journal of …, 2012 - Elsevier... The spoligotypes were compared with the most prevalent MTB subfamilies as identified by the World Spoligotyping Database SpolDB4.0 of the Pasteur Institute, Guadeloupe. 18. ... 3.4. Genotypicpattern of MTB isolates. Of the 1004 isolates, 987 were spoligotyped. ...All 2 versions - Import into BibTeX

IMPLICATION OF THE RD< SUP> RIO</SUP>< I> MYCOBACTERIUM TUBERCULOSIS</I> SUBLINEAGE IN MULTIDRUG RESISTANT TUBERCULOSIS IN PORTUGAL

S David, EL Duarte, CQF Leite, JN Ribeiro… - Infection, Genetics and …, 2012 - Elsevier... patient), were genotyped by spoligotyping according to the manufacturer's instructions (Isogen Bioscience BV ... Contribution of Spoligotyping to the Characterization of the population structure of ... Update on the Spoligotypes of Mycobacterium tuberculosis complex isolates from the ...Import into BibTeX

[PDF] ÇUKUROVA BÖLGESINDE AKCIĞER TÜBERKÜLOZLU HASTALARDAN İZOLE EDILEN MYCOBACTERIUM TUBERCULOSIS SUŞLARININ SPOLIGOTIPLENDIRME VE MIRU-VNTR YÖNTEMIYLE …

ÜO ZEYTİNLİ… - Mikrobiyol Bul, 2012 - mikrobiyolbul.org... Key words: Mycobacterium tuberculosis; spoligotyping; MIRU-VNTR; molecular epidemiology; Turkey. GİRİŞ ... Spoligotiplendirme Spoligotiplendirme yöntemi, üretici firmanın önerileri (Isogen Bioscience BV, Hollan- da) dikkate alınarak uygulandı. ...View as HTML - Import into BibTeX

Rapid spoligotyping of Mycobacterium tuberculosis complex bacteria using a microarray system with automatic data processing and assignment

A Ruettger, J Nieter, A Skrypnyk… - Journal of Clinical …, 2012 - Am Soc Microbiol... Short-Form Paper 4 reverse line blot hybridization using the Spoligotyping Kit (Ocimum Biosolutions, Hydera- 79 bad, India) and the present DNA microarray. ... ArrayStrip spoligotyping and the assay's capability of identifying new spoligotypes and li- 109 neages. ...Import into BibTeX

Rapid Test for Identification of a Highly Transmissible Mycobacterium tuberculosis Beijing Strain of Sub-Saharan Origin

MI Millán-Lou, H Alonso, P Gavín… - Journal of Clinical …, 2012 - Am Soc Microbiol... First, all 292 isolates were screened by spoligotyping with a commercially available kit (Ocimum Biosolutions Ltd., Hyderabad, India) by the method of Kamerbeek et al. (14). Seventy of the 292 isolates belonged to the Beijing family. ...Related articles - All 3 versions - Import into BibTeX

 

GenoType MTBDRsl performance on clinical samples with diverse genetic background

P Miotto, AM Cabibbe, P Mantegani… - European …, 2012 - Eur Respiratory Soc ... Spoligotyping. The amplified direct repeat locus of clinical isolates was investigated using the Spoligotyping kit (Ocimum Biosolutions, Hyderabad, India) following the manufacturer's instructions. Spoligotyping results were entered, in binary format, in the MIRU-VNTRplus web ...Related articles - All 2 versions - Import into BibTeX

New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Mycobacterium tuberculosis</i> strains from Saudi Arabia

B Varghese, A Hillemann, DR Wijayanti… - Infection, Genetics and …, 2012 - Elsevier... Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typing and spoligotyping. Spoligotyping was performed as previously described (Kamerbeek et al., 1997) with the commercial kit (Ocimum Biosolutions, Hyderabad, India). ...All 2 versions - Import into BibTeX

 [HTML] DISTRIBUTION OF SPOLIGOTYPING DEFINED GENOTYPIC LINEAGES AMONG DRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS COMPLEX CLINICAL ISOLATES IN ANKARA, TURKEY

[HTML] from plos.org

O Kisa, G Tarhan, S Gunal, A Albay, R Durmaz… - PloS one, 2012 - dx.plos.org... Spoligotyping was carried out with a commercial kit (Isogen Bioscience BV, Maarssen, TheNetherlands ... tuberculosis H37Rv and M.bovis BCG P3 were used as control for spoligotyping.Spoligotypes results were converted into octal code and in the SITVIT2 proprietary database ...Related articles - Cached - All 4 versions - Import into BibTeX

[HTML] SPOLIGOTYPING AND DRUG RESISTANCE ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS FROM NATIONAL SURVEY IN CHINA

Y Pang, Y Zhou, B Zhao, G Liu, G Jiang, H Xia… - PloS one, 2012 - dx.plos.org... A commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used asdescribed by ... of the direct repeat region of Mycobacterium tuberculosis: implications for interpretation of spoligotyping data. ... 2004) Predominant tuberculosis spoligotypes, Delhi, India. ...Cached - Import into BibTeX

[HTML] SPOLIGOTYPING AND VARIABLE NUMBER TANDEM REPEAT ANALYSIS OF MYCOBACTERIUM BOVIS ISOLATES FROM CATTLE IN BRAZIL

PM Parreiras, GI Andrade… - Memórias do Instituto …, 2012 - SciELO Brasil... The PCR products were hybridised to a membrane (Isogen, The Netherlands) containing 43oligonucleotides of known spacer sequences by reversed-line blot hybridisation. ... Spoligotyping -Complete spoligotypes were obtained from 61 isolates (Table I). In total, 17 ...Related articles - Cached - All 5 versions - Import into BibTeX

EVALUATION OF 24-LOCUS MIRU-VNTR IN EXTRAPULMONARY SPECIMENS: STUDY FROM A TERTIARY CENTRE IN MUMBAI

V Vadwai, A Shetty, P Supply… - Tuberculosis, 2012 - Elsevier... Spoligotyping, was performed using a commercial kit (Isogen, Netherlands) as per the ... 13/69,18.8%) spoligo-prototypes, compared to LAM, Haarlem or T spoligotypes represented at ... Analysisof the congruence between MIRU-VNTR and spoligotyping results combined with the ...Cited by 1 - All 3 versions - Import into BibTeX

EPIDEMIOLOGICAL SIGNIFICANCE OF THE DOMESTIC BLACK PIG (SUS SCROFA) IN THE MAINTENANCE OF BOVINE TUBERCULOSIS IN SICILY

V Di Marco, P Mazzone, MT Capucchio… - Journal of Clinical …, 2012 - Am Soc Microbiol... direct repeat locus were detected by hybridization onto a spoligotyping membrane (Isogen 231 ...M. bovis isolates from both field studies were genotyped by both spoligotyping 342 ... Four spoligotypes were identified: SB0120 (23 isolates), SB0841 (five isolates), SB0833 (two 344 ...Related articles - All 2 versions - Import into BibTeX

TRANSMISSION OF TUBERCULOSIS WITHIN FAMILY-HOUSEHOLDS

E Augustynowicz-Kopeć, T Jagielski, M Kozińska… - Journal of Infection, 2012 - Elsevier... Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen ...had different M. tuberculosis isolates, as evidenced by both spoligotyping and MIRU ... Assumingisolates revealing both identical spoligotypes and VNTR profiles are representing the ...All 2 versions - Import into BibTeX

[PDF] PREVALENCE OF PULMONARY TB AND SPOLIGOTYPE PATTERN OF MYCOBACTERIUM TUBERCULOSIS AMONG TB SUSPECTS IN A RURAL COMMUNITY IN SOUTHWEST ETHIOPIA

A Deribew, G Abebe, L Apers… - BMC Infectious …, 2012 - biomedcentral.com... Standard spoligotyping method [12] was done generally as described by Kamerbeek andcolleagues using a commercially available kit (Isogen Bioscience BV ... Spoligotypes described only once (non-clustered) in this study and in the SpolDB4 were designated as ...View as HTML - Import into BibTeX

CHARACTERIZING< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES FROM KARACHI, PAKISTAN: DRUG RESISTANCE AND GENOTYPES

A Ayaz, Z Hasan, S Jafri, R Inayat, R Mangi… - International Journal of …, 2012 - Elsevier... The spoligotypes were compared with the most prevalent MTB subfamilies as identified by the World Spoligotyping Database SpolDB4.0 of the Pasteur Institute, Guadeloupe. 18. ... 3.4. Genotypic pattern of MTB isolates. Of the 1004 isolates, 987 were spoligotyped. ...All 2 versions - Import into BibTeX

IMPLICATION OF THE RD< SUP> RIO</SUP>< I> MYCOBACTERIUM TUBERCULOSIS</I> SUBLINEAGE IN MULTIDRUG RESISTANT TUBERCULOSIS IN PORTUGAL

S David, EL Duarte, CQF Leite, JN Ribeiro… - Infection, Genetics and …, 2012 - Elsevier... patient), were genotyped by spoligotyping according to the manufacturer's instructions (Isogen Bioscience BV ... Contribution of Spoligotyping to the Characterization of the population structure of ... Update on the Spoligotypes of Mycobacterium tuberculosis complex isolates from the ...Import into BibTeX

[PDF] ÇUKUROVA BÖLGESINDE AKCIĞER TÜBERKÜLOZLU HASTALARDAN İZOLE EDILEN MYCOBACTERIUM TUBERCULOSIS SUŞLARININ SPOLIGOTIPLENDIRME VE MIRU-VNTR YÖNTEMIYLE …

ÜO ZEYTİNLİ… - Mikrobiyol Bul, 2012 - mikrobiyolbul.org... Key words: Mycobacterium tuberculosis; spoligotyping; MIRU-VNTR; molecular epidemiology;Turkey. GİRİŞ ... Spoligotiplendirme Spoligotiplendirme yöntemi, üretici firmanın önerileri(Isogen Bioscience BV, Hollan- da) dikkate alınarak uygulandı. ...View as HTML - Import into BibTeX

 

2011

 

[HTML] Bovine tuberculosis in a Nebraska herd of farmed elk and fallow deer: a failure of the tuberculin skin test and opportunities for serodiagnosis

W Waters, GE Stevens, MA Schoenbaum… - Veterinary medicine …, 2011 - hindawi.com... Isolates of M. bovis were identified by a combination of Ziehl-Neelsen acid-fast staining, nucleic acid probes (AccuProbe, Gen-Probe, San Diego, CA, USA), and spoligotyping (Ocimum Biosolutions Ltd., Hyderabad, India). Identification of atypical Mycobacteria spp. …Cited by 2 - Related articles - Cached - All 13 versions - Import into BibTeX

 [HTML] The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines …[HTML] from biomedcentral.com

E Abadia, J Zhang, V Ritacco… - BMC infectious …, 2011 - biomedcentral.com... of hard to interpret spoligotypes and samples representative of the work flow, so we could evaluate the benefits of a new platform regarding the classical one on these samples. Most centers had performed spoligotyping using commercial membranes (Ocimum, Hyderabad, India ...Cited by 3 - Related articles - Cached - All 26 versions - Import into BibTeX

Quality assurance for molecular epidemiology of tuberculosis methods in the mycobacterum reference laboratory

PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… - … and Quality Assurance: …, 2011 - Springer... for spoli- gotyping results, standardization and reproducibility depend on the quality of the commercial spoligotyping kit (Ocimum). ... Spoligotyping Pattern ... Both PETRA (a MIRU-VNTR lineage with central asian spoligotypes, CAS) and Beijing clusters have been observed in other ...Related articles - All 3 versions - Import into BibTeX

 

DETERMINATION OF DRUG SUSCEPTIBILITY PATTERNS AND GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM KANPUR DISTRICT; …S Purwar, S Chaudhari, VM Katoch… - Infection, Genetics and …, 2011 - Elsevier... commercially available kit 126 (Isogen Biosciences , BV, Maarsen, The Netherlands now Ocimum Biosolutions) and ... Owing to small sample size, the exact prevalence of various 272 spoligotypes and M ... Out of the isolates clustered by spoligotyping, all 280 were unique strains by ...Cited by 3 - Related articles - All 4 versions - Import into BibTeX

 

New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Mycobacterium tuberculosis</i> strains from Saudi ArabiaB Varghese, A Hillemann, DR Wijayanti… - Infection, Genetics and …, 2012 - Elsevier... Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typing and spoligotyping. Spoligotyping was performed as previously described (Kamerbeek et al., 1997) with the commercial kit (Ocimum Biosolutions, Hyderabad, India). ...All 2 versions - Import into BibTeX

 

High prevalence of subclinical tuberculosis in HIV-1-infected persons without advanced immunodeficiency: implications for TB screening

T Oni, R Burke, R Tsekela, N Bangani, R Seldon… - Thorax, 2011 - thorax.highwire.org... Lowenstein–Jensen slope cultures. The DNA was used to perform spoligotyping as previously described4 using a commercially available kit from Ocimum Biosolutions BV, The Netherlands. The hybridisation patterns obtained ...Cited by 1 - Related articles - All 12 versions - Import into BibTeX

[HTML] Pathology of camel tuberculosis and molecular characterization of its causative agents in pastoral regions of Ethiopia

G Mamo, G Bayleyegn, TS Tessema, M Legesse… - PloS one, 2011 - dx.plos.org... Spoligotyping. Spoligotyping was performed as previously described by Kamerbeek and coauthors [22] and according to the spoligotype kit supplier's instructions (Ocimum Biosolutions Company, Iisselstein, The Netherlands). ...Cited by 3 - Related articles - Cached - All 9 versions - Import into BibTeX

 

POPULATION-BASED INVESTIGATION OF FLUOROQUINOLONES RESISTANT TUBERCULOSIS IN RURAL EASTERN CHINA Y Hu, B Mathema, W Wang, B Kreiswirth, W Jiang… - Tuberculosis, 2011 - Elsevier... Spoligotyping was carried out using commercial kits from Isogen Bioscience BV (Maarssen, The Netherlands). 15 Strain family was determined by comparison of spoligotyping patterns with the SpoligDB4 database and assigned a spoligotyping international type (SIT). …Cited by 2 - Related articles - All 4 versions - Import into BibTeX

 

[HTML] THE GUINEA-BISSAU FAMILY OF MYCOBACTERIUM TUBERCULOSIS COMPLEX REVISITED [HTML] from plos.org R Groenheit, S Ghebremichael, J Svensson… - PLoS One, 2011 - dx.plos.org... All isolates that were subjected to RFLP were also spoligotyped. ... Altogether, 414 isolates were available for spoligotyping, of which 336 isolates were grouped into 42 clusters ... Out of 229 isolates, 82 different spoligotypes were obtained, of which 168 (73.4%) were clustered into ...Cited by 4 - Related articles - Cached - All 5 versions - Import into BibTeX

 

GENETIC DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS IN MBARARA, SOUTH WESTERN UGANDA [PDF] from ajol.infoBazira, M Matte, BB Asiimwe… - African health sciences, 2011 - ajol.info... was performed as previously described29 using a commercially available kit (Isogen Bioscience BV ... Spoligotypes that did not match any existing pattern in the SpolDB4 database were ... tuberculosis within the Mbarara, we have used RD analysis and spoligotyping to characterize ...Related articles - All 8 versions - Import into BibTeX

FLUOROQUINOLONE RESISTANCE AMONG MYCOBACTERIUM TUBERCULOSIS STRAINS FROM KARACHI, PAKISTAN: DATA FROM COMMUNITY-BASED FIELD CLINICS TML] from nih.gov  Rafiq, K Jabeen, R Hasan, S Jafri… - Antimicrobial agents …, 2011 - Am Soc Microbiol... was performed on all FQ-resistant strains using a commercially available kit provided by Isogen Life Science BV ... The spoligotypes were compared with the most prevalent M. tuberculosis subfamilies, as identified by the world spoligotyping database, SpolDB4.0, of ...Related articles - All 6 versions - Import into BibTeX

[HTML] CHARACTERISTICS OF PNCA MUTATIONS IN MULTIDRUG-RESISTANT TUBERCULOSIS IN TAIWAN [HTML] from biomedcentral.com YC Chiu, SF Huang, KW Yu, YC Lee… - BMC infectious …, 2011 - biomedcentral.com... Genotyping. All clinical isolates were genotyped by a commercial spoligotyping kit (Isogen Bioscience BV, Maarssen, Netherlands). The "Beijing strain" was defined as deletion from spacer 1 to spacer 34 in the direct repeat region and the presence of (at least 3) spacers 35-43. ...Related articles - Cached - All 11 versions - Import into BibTeX

[HTML] INITIAL PRESENTATIONS PREDICT MORTALITY IN PULMONARY TUBERCULOSIS PATIENTS-A PROSPECTIVE OBSERVATIONAL STUDY [HTML] from plos.org JY Feng, WJ Su, YC Chiu, SF Huang, YY Lin… - PloS one, 2011 - dx.plos.org... All clinical isolates were genotyped using a commercial spoligotyping kit (Isogen Bioscience BV, Maarssen, Netherlands). The “Beijing strain” was defined as a deletion from spacer 1 to spacer 34 in the direct repeat region and the presence of (at least 3) spacers in 35–43. ...Related articles - Cached - All 4 versions - Import into BibTeX

[HTML] MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS, BUENOS AIRES, ARGENTINA [HTML] from nih.govX Gonzalo, M Ambroggi, E Cordova… - Emerging Infectious …, 2011 - ncbi.nlm.nih.gov... DNA was extracted from cultures at this UK laboratory; spoligotyping was performed according to the manufacturer's instructions (Isogen Life Science, IJsselstein, the Netherlands); and data were analyzed with SPOTCLUST (http://cgi2.cs.rpi.edu/~bennek/SPOTCLUST.html). ...Related articles - All 4 versions - Import into BibTeX

CHARACTERISTICS OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS IN TAIWAN: A POPULATION-BASED STUDY CW Chang, MH Wu, PC Chuang… - Infection, Genetics and Evolution, 2011 - Elsevier... 12 13 Spoligotyping 14 A commercially available kit (Isogen Bioscience BV, Maarssen, The 15 Netherlands) was used as ... Even through spoligotyping results 3 revealed the genetic diversities of MDR M. tuberculosis isolates in Taiwan, 4 identical spoligotypes could not ...Cited by 2 - Related articles - All 2 versions - Import into BibTeX

MOLECULAR PATTERN OF MYCOBACTERIUM BOVIS ISOLATES AND ITS RELATIONSHIP WITH RISK FACTORS ASSOCIATED WITH THE PRESENCE OF BOVINE …[PDF] from uaemex.mx IF Padilla-Campos, JC Segura-Correa… - Tropical and …, 2011 - redalyc.uaemex.mx... Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors. ... Spoligotyping was carried out following the protocol described by Kamerbeek et al ... sequences from M. tuberculosis and six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The ...Related articles - All 2 versions - Import into BibTeX

[HTML] FINE-TUNING THE SPACE, TIME, AND HOST DISTRIBUTION OF MYCOBACTERIA IN WILDLIFE[HTML] from biomedcentral.com C Gortazar, MJ Torres, P Acevedo, J Aznar… - BMC …, 2011 - biomedcentral.com... Typing. The M. bovis isolates were further characterized by spoligotyping [40]. The amplified product was detected by hybridization of the biotin-labelled PCR product onto spoligotyping membrane (Isogen Bioscience BV, Maarssen, The Netherlands). ...Cited by 6 - Related articles - Cached - All 19 versions - Import into BibTeX

MOLECULAR PATTERN OF MYCOBACTERIUM BOVIS ISOLATES AND ITS RELATIONSHIP WITH RISK FACTORS ASSOCIATED WITH THE PRESENCE OF …[PDF] from uady.mx IF Padilla, JC Segura, JC Rodriguez… - Tropical and …, 2011 - ccba.uady.mx... Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors. ... Spoligotyping was carried out following the protocol described by Kamerbeek et al ... sequences from M. tuberculosis and six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The ...Related articles - All 2 versions - Import into BibTeX

EPIDEMIOLOGICAL TRENDS AND CLINICAL COMPARISONS OF< I> MYCOBACTERIUM</I>< I> TUBERCULOSIS</I> LINEAGES IN THAI TB MENINGITISK Faksri, F Drobniewski, V Nikolayevskyy, T Brown… - Tuberculosis, 2011 - Elsevier... isolated from CSF samples collected from 1996 to 2004 with IS6110 RFLPs (120 RFLP patterns were available) and spoligotyping results (141 spoligotypes were available ... 27 Spoligotypingwas performed according to the manufacturer's instructions (Isogen Bioscience BV ...Cited by 1 - Related articles - All 3 versions - Import into BibTeX

A NOVEL METHOD FOR THE RAPID AND PROSPECTIVE IDENTIFICATION OF BEIJING MYCOBACTERIUM TUBERCULOSIS STRAINS BY HIGH‐RESOLUTION MELTING ANALYSISAlonso, Y Navarro, F Barletta… - Clinical …, 2011 - Wiley Online Library... Thirty-two MTB strains (18 representatives of the Beijing genotype and 14 representatives of the non-Beijing genotype, according to their spoligotypes) were grown on ... Spoligotyping was performed in accordance with the manufacturer's instructions (Isogen, De Meern, the ...Cited by 3 - Related articles - All 3 versions - Import into BibTeX

QUALITY ASSURANCE FOR MOLECULAR EPIDEMIOLOGY OF TUBERCULOSIS METHODS IN THE MYCOBACTERIUM REFERENCE LABORATORY PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… - … and Quality Assurance: …, 2011 - Springer... 43 spacer spoligotyping was performed according to the manufacturer's instructions (Isogen/Ocimun) as described previously. ... 27 Spoligotyping Pattern ... Both PETRA (a MIRU-VNTR lineage with central asian spoligotypes, CAS) and Beijing clusters have been observed in other ...Related articles - All 3 versions - Import into BibTeX

EPIDEMIOLOGICAL ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED IN LODZ, POLANDKrawczyk, A Brzostek, A Gorna… - … of Tuberculosis and …, 2011 - ingentaconnect.com... of van Embden et al.16 Spoligotyping was performed as described by Kamerbeek et al., using the Isogen Life Science kit ... Spoligotyping analysis showed that 184 (78.6%) of the strains were grouped in clusters and 50 (21.4%) strains possessed unique spoligotypes. ...

 

ONE YEAR NATIONWIDE EVALUATION OF 24-LOCUS MIRU-VNTR GENOTYPING ON SLOVENIAN< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES U Bidovec-Stojkovic, M Zolnir-Dovc… - Respiratory Medicine, 2011 - Elsevier... 4,9,20 Spoligotyping was performed using a commercially available kit (Isogen Bioscience B identically defined by IS6110 RFLP and 24-locus MIRU-VNTR typing combined with spoligotyping. ...jointly defined by identical 24-locus MIRU-VNTR types and spoligotypes were only ...

 

MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX (MTBC) ISOLATED FROM CATTLE IN NORTHEAST AND NORTHWEST CHINA Y Du, Y Qi, L Yu, J Lin, S Liu, H Ni… - Research in veterinary …, 2011 - Elsevier... 2.5. Spoligotyping. Spoligotyping kits and Mini-blotter MN45 were obtained from Isogen Life Science Co. (Utrecht, the Netherlands). The Streptavidin-ß-peroxidase conjugate was produced by Roche Applied Science Co. (Penzberg, Germany). ...Cited by 3 - Related articles - All 3 versions - Import into BibTeX

[HTML] TRANSMISSION PATTERN OF DRUG-RESISTANT TUBERCULOSIS AND ITS IMPLICATION FOR TUBERCULOSIS CONTROL IN EASTERN RURAL CHINA [HTML] from plos.org Y Hu, B Mathema, W Jiang, B Kreiswirth, W Wang… - PloS one, 2011 - dx.plos.org... Hybridized DNA was detected using an enhanced chemiluminescene kit from Isogen Bioscience BV ... b Probaiblity that the Spoligotyping pattern belongs to the clades. ... N, Bennett KP (2006) Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. ...Related articles - Cached - All 7 versions - Import into BibTeX

SUBLINEAGES OF BEIJING STRAIN OF MYCOBACTERIUM TUBERCULOSIS IN SRI LANKA US Rajapaksa… - Indian Journal of Microbiology, 2011 - Springer... Spoligotyping was carried out according to manufacturer's guidelines using a commercially available kit (Isogen, Maarsen MV, The Netherland). Fourteen iso-lates that confirmed to be Beijing strain were further characterised. ...Related articles - All 2 versions - Import into BibTeX

DETERMINATION OF DRUG SUSCEPTIBILITY PATTERNS AND GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM KANPUR DISTRICT; …S Purwar, S Chaudhari, VM Katoch… - Infection, Genetics and …, 2011 - Elsevier... 43 spacers was done with the help of a commercially available kit 126 (Isogen Biosciences ,BV ... Owing to small sample size, the exact prevalence of various 272 spoligotypes and M.tuberculosis ... Out of the isolates clustered by spoligotyping, all 280 were unique strains by IS6110 ...Cited by 3 - Related articles - All 4 versions - Import into BibTeX

MOLECULAR EPIDEMIOLOGY OF HUMAN AND ANIMAL TUBERCULOSIS IN IBADAN, SOUTHWESTERN NIGERIA AO Jenkins, SIB Cadmus, EH Venter… - Veterinary …, 2011 - Elsevier... Spoligotyping167 Spoligotyping confirmed the species identification of 19 M. tuberculosis and 13 M ... None of these CAM 173 variant spoligotypes were found in the spolDB4 database and were ...All M. tuberculosis strains 175 from PTB patients that were spoligotyped had identical ...Cited by 1 - Related articles - All 5 versions - Import into BibTeX

 

GENETIC DIVERSITY OF THE MYCOBACTERIUM TUBERCULOSIS BEIJING FAMILY BASED ON IS6110, SNP, LSP AND VNTR PROFILES FROM THAILANDK Faksri, F Drobniewski, V Nikolayevskyy… - Infection, Genetics and …, 2011 - Elsevier... Eighty MTB Beijing isolates from CSF samples with IS6110 RFLPs (69 RFLP patterns wereavailable) and spoligotyping results (all 80 spoligotypes were available ... Spoligotyping was performed according to the manufacturer s instructions (Isogen Bioscience BV ...Cited by 2 - Related articles - All 3 versions - Import into BibTeX

[PDF] 骨结核临床分离菌株基因分型的特点 [PDF] from clinicmed.net李文胜, 刘毅, 孙勇, 田苗, 刘忠泉… - 中华临床医师杂志 ( …, 2011 - clinicmed.net... TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自TaKaRa 公司。凝胶成像仪购自FOTO DYNE 公司。 Spoligotyping 试 剂盒以及Miniblotter MN45为荷兰 Isogen Bioscience 公司产品。 链霉亲和素为Sigam 公司产品。 化学发光 ...Related articles - View as HTML - All 3 versions - Import into BibTeX

MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS IN THE UNITED ARAB EMIRATES [PDF] from qmul.ac.uk A Saedi… - 2011 - qmro.qmul.ac.uk ... 130 3.2.11 Identification of the UAE isolates by spoligotyping..... 132 3.2.12 Comparison betweenMIRU-typed and spoligotyped families in association with katG463/gyrA95 groupings ..... … Related articles - All 2 versions - Import into BibTeX 

 

[HTML] MYCOBACTERIUM BOVIS IN SWINE: SPOLIGOTYPING OF ISOLATES FROM ARGENTINA [HTML] from hindawi.comS Barandiaran, EV Moras, A Adri& - Veterinary Medicine …, 2011 - hindawi.com... Spoligotyping [14] was carried out by using the spoligotyping kit (Isogen Biosolutions BV, Ocimun ...Guerin (BCG) (ATCC 27289) were included as reference strains in each spoligotyping experiment ...isolates were defined as two or more M. bovis strains with identical spoligotypes. ...Cited by 1 - Related articles - Cached - All 11 versions - Import into BibTeX

[HTML] SPOLIGOTYPING OF CLINICAL MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM THE STATE OF MINAS GERAIS, BRAZIL [HTML] from scielo.brSS Miranda, WS Carvalho, PN Suffys… - Memórias do Instituto …, 2011 - SciELO Brasil... was resuspended in the same TE buffer, stored at -20ºC and subjected to spoligotyping using a previously published protocol (Kamerbeek et al. 1997) and a commercially available kit (Isogen Life Science BV, Utrecht, The Netherlands). The hybridised spoligotypes in binary ...Related articles - Cached - All 7 versions - Import into BibTeX

EVALUATION OF SPOLIGOTYPING AND MIRU-VNTR FOR MYCOBACTERIUM BOVIS IN XINJIANG, CHINAZ Sun, R Cao, M Tian, X Zhang, X Zhang… - Research in Veterinary …, 2011 - Elsevier... Additionally, two new spoligotypes (SB1903 and SB1904) and special repeat numbers of 3 loci (ETR ... Spoligotyping was firstly analyzed and interpreted in octal codes (Dale et al., 2001) following the instruction (http://www.mbovis.org) with a commercial kit (Isogen Bioscience BV ...Cited by 2 - Related articles - All 2 versions - Import into BibTeX

[HTML] MYCOBACTERIUM TUBERCULOSIS SPOLIGOTYPES AND DRUG SUSCEPTIBILITY PATTERN OF ISOLATES FROM TUBERCULOSIS PATIENTS IN SOUTH-WESTERN UGANDA [HTML] from biomedcentral.comJ Bazira, BB Asiimwe, ML Joloba… - BMC infectious …, 2011 - biomedcentral.com... Spoligotyping. Standard spoligotyping was performed as previously described [29] using a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands). ... Spoligotyping.All the isolates had spoligotypes characteristic of M. tuberculosis. ...Cited by 1 - Related articles - Cached - All 12 versions - Import into BibTeX

A FIRST INSIGHT ON THE POPULATION STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS COMPLEX AS STUDIED BYSPOLIGOTYPING AND MIRU-VNTRS IN BOGOTÁ, COLOMBIAI Cerezo, Y Jiménez, J Hernandez, T Zozio… - Infection, Genetics and …, 2011 - Elsevier... Kamerbeek et al., 1997) according to the manufacturer s instructions (Isogen Bioscience, the ...Spoligotype International Type (SIT) and MIRU International Type (MIT) designate spoligotypes and MIRU ... was used to build minimum spanning trees based on spoligotyping and MIRU ...All 2 versions - Import into BibTeX

[PDF] PRELIMINARY MAPPING STUDY OF MYCOBACTERIUM TUBERCULOSIS' COMPLEX DNA BYSPOLIGOTYPING ON ISOLATED SPUTUM OF TUBERCULOSIS PATIENT …[PDF] from digitaljournals.org V Lisdawati, I Parwati… - Bulletin of Health …, 2011 - indonesia.digitaljournals.org... PCR, denaturasi menggunakan thermocycler selama 10 menit pada suhu 99,9°C. Kemudian pada mini blotter MN45 yang telah diletakkan membran spoligotyping kit (IM9702, Isogen Biosolution, BV) berlabel 43 DNA spacer Mtb complex segera hibridisasikan DNA hasil PCR. ...Related articles - View as HTML - Import into BibTeX

[PDF] INFECTION OF EURASIAN BADGERS (MELES MELES) WITH MYCOBACTERIUM BOVIS[PDF] from uclm.esA Balseiro, O Rodríguez, P González-Quirós… - prevalence, 2011 - uclm.es... al., 2009). 125 MTC isolates were characterised hybridisation of biotin-labelled PCR products 126 onto a spoligotyping membrane (Isogen Bioscience BV), followed by spoligotyping 127 (Kamerbeek et al., 1997). Results were ...Cited by 2 - Related articles - View as HTML - All 2 versions - Import into BibTeX

CLINICAL AND MICROBIOLOGICAL CHARACTERISTICS OF URINE CULTURE-CONFIRMED GENITOURINARY TUBERCULOSIS AT MEDICAL CENTERS IN TAIWAN FROM 1995 TO 2007HL Hsu, CC Lai, MC Yu, FL Yu, JC Lee… - European journal of …, 2011 - Springer... commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as described by the manufacturer. The fourth international spoligotyping database (SpolDB4) database was used to assign genotype families to the spoligotypes obtained [14 ...Related articles - All 5 versions - Import into BibTeX

MOLECULAR TYPING OF MYCOBACTERIUM TUBERCULOSIS CIRCULATED IN MOSCOW, RUSSIAN FEDERATIONMV Afanas' ev, LN Ikryannikova, EN Il'ina… - European Journal of …, 2011 - Springer... spacers probes from the spoligotyping kit from Isogen Lifescience (cat. no. IM9701). 182 Eur J Clin Microbiol Infect Dis (2011) 30:181–191 Page 3. Spoligotypes in binary format were entered in an Excel spreadsheet and compared with the spoligotyping database SpolDB4 [13]. ...Cited by 7 - Related articles - All 4 versions - Import into BibTeX

ROLE OF 2-MONTH SPUTUM SMEARS IN PREDICTING CULTURE CONVERSION IN PULMONARY TUBERCULOSIS[HTML] from ersj.org.uk WJ Su, JY Feng, YC Chiu… - European Respiratory …, 2011 - Eur Respiratory Soc... All clinical isolates were genotyped by commercial spoligotyping kit (Isogen Bioscience BV, Maarssen, the Netherlands). The “Beijing strain” was defined as deletion from spacer 1 to spacer 34 in the direct repeat region and the presence of (at least three of) spacers 35–43. ...Cited by 5 - Related articles - All 6 versions - Import into BibTeX

[PDF] A TWO-STEP STRATEGY FOR MOLECULAR TYPING OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATES FROM POLAND. [PDF] from microbiology.pl T JAGIELSKI, EWAA KOPEĆ… - Polish journal of …, 2011 - pjm.microbiology.pl... Spoligotyping was perfor- med with a commercially available kit (Isogen Bio- science BV ... e isolates whose spoligotype patterns were already recorded in the database, were assigned“shared types”, whereas those of spoligotypes identified for the first time, were ...View as HTML - All 3 versions - Import into BibTeX

MOLECULAR TECHNOLOGIES USED IN DETECTING OF SENSITIVE AND ISONIAZID-RESISTANT MYCOBACTERIUM TUBERCULOSIS OY Limanskaya… - Cytology and Genetics, 2011 - Springer... The PCR was carried out by a dry kit of reagents for DNA amplification (Isogen, Russian Federation) in 20–50 μl of reaction mixture containing 4 mM NaCl, 50 mM KCl, 12 mM Tris HCl (pH 8.0), 2.5 mM MgCl2, 200 μM dNTPs, 0.1–0.5 μM of each primer, and 1 unit of Taq DNA ...Related articles - All 4 versions - Import into BibTeX

STREPTOMYCIN RESISTANCE AND LINEAGE-SPECIFIC POLYMORPHISMS IN MYCOBACTERIUM TUBERCULOSIS GIDB GENE HTML] from nih.govFS Spies, AW Ribeiro, DF Ramos… - Journal of clinical …, 2011 - Am Soc Microbiol... Genotyping.Spoligotyping was performed using a commercial kit (Isogen Biosciences BV, Netherlands ... it was classified as LAM3 and S/convergent sublineage by spoligotyping but as non … Spoligotypes evolve through successive loss of spacer DNA sequences (16), and since ...Cited by 2 - Related articles - All 7 versions - Import into BibTeX

GENOTYPING AND DRUG RESISTANCE PATTERNS OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN FIVE PROVINCES OF CHINA YL Guo, Y Liu, SM Wang, CY Li… - … of Tuberculosis and …, 2011 - ingentaconnect.com... Of the (Isogen Bioscience BV, De Meern, The Netherlands). ... 1–1800 = shared spoligotypes. Table 3 Results of MIRU typing and comparison of the three typing methods Isolate MIRU locus number IS6110-RFLP Cluster 2 4 10 16 20 23 24 26 27 31 39 40 Spoligotyping MIRU ...Cited by 3 - Related articles - All 3 versions - Import into BibTeX

ASSOCIATION OF< I> MYCOBACTERIUM TUBERCULOSIS</I> GENOTYPES AND CLINICAL AND EPIDEMIOLOGICAL FEATURES-A MULTI-CENTER STUDY IN TAIWAN SF Huang, WJ Su, HY Dou, JY Feng… - Infection, Genetics and …, 2011 - Elsevier... Spoligotyping was performed according to the method reported by Kamerbeek et al. (Kamerbeek et al., 1997) using a commercial kit (Isogen Biosolutions, Netherlands). The spoligotypes were identified according to the database SpoDB4.0 (Brudey et al., 2006), and the SIT ...Related articles - All 5 versions - Import into BibTeX

THE GENOTYPE CHARACTERISTIC OF CLINICAL ISOLATED STRAINS GETTING FROM BONE TUBERCULOSIS[PDF] from clinicmed.netLI Wen-sheng, LIU Yi, SUN Yong… - Chinese Journal of …, 2011 - global.clinicmed.net... TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自 TaKaRa 公司。 凝胶成像仪购自 FOTO DYNE 公司。 Spoligotyping 试 剂盒以及 Miniblotter MN45为荷兰 Isogen Bioscience 公司产品。 链霉亲和素为 Sigam 公司产品。 化学发光 ...Related articles - Import into BibTeX

MULTIPLE STRAINS OF< I> MYCOBACTERIUM BOVIS</I> REVEALED BY MOLECULAR TYPING IN A HERD OF CATTLE EES Figueiredo, DF Ramos, L Medeiros… - The Veterinary …, 2011 - Elsevier... to the spoligo-membrane was performed according to the manufacturer's instructions (Isogen Life Sciences). ... The strains clustered by spoligotyping were analysed by VNTR-PCR to confirm clonal relationships ... Four spoligotypes were identified among the 12 M. bovis isolates. ...Related articles - All 2 versions - Import into BibTeX

DETERMINAÇÃO DO CUSTO BIOLÓGICO EM ISOLADOS DE MYCOBACTERIUM TUBERCULOSIS COM MUTAÇÕES NOS GENES RPSL, RRS, GIDB, RPOB E KATG [PDF] from ufrgs.br FS Spies - 2011 - lume.ufrgs.br... restriction fragment lenght polymorphism) da sequência de inserção IS6110, spoligotyping e unidade de repetição de micobactéria- repetição em tandem de número variável (MIRU- ...Spoligotyping é uma técnica de genotipagem que explora a variabilidade da região ...Related articles - Import into BibTeX

A MAJOR SUBGROUP OF BEIJING FAMILY MYCOBACTERIUM TUBERCULOSIS IS ASSOCIATED WITH MULTIDRUG RESISTANCE AND INCREASED TRANSMISSIBILITY Y Hu, X Ma, EA Graviss, W Wang… - Epidemiology and …, 2011 - Cambridge Univ Press... Spoligotyping was performed by using a com- mercial kit from Isogen Bioscience BV (Maarssen ...0.04), and resistance to RIF and SM (P=0.04) (Table 1). Spoligotyping identified 243 ... Thirty-three(9.4%) isolates had unique/novel spoligotypes, of which similarity analysis found that ...Cited by 6 - Related articles - All 4 versions - Import into BibTeX

[HTML] MODERN AND ANCESTRAL GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS FROM ANDHRA PRADESH, INDIA [HTML] from plos.org SK Thomas, CC Iravatham, BH Moni, A Kumar… - PloS one, 2011 - dx.plos.org ... the help of commercially available line probe arrays (Isogen Biosciences BV ... tuberculosis complexgenetic diversity: mining the fourth international spoligotyping database (SpolDB4 ... KP (2006)Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. ...Related articles - Cached - All 5 versions - Import into BibTeX

[HTML] TUBERCULOSIS IN SUDAN: A STUDY OF MYCOBACTERIUM TUBERCULOSIS STRAIN GENOTYPE AND SUSCEPTIBILITY TO ANTI-TUBERCULOSIS DRUGS [HTML] from biomedcentral.com GSS Eldin, I Fadl-Elmula, MS Ali… - BMC infectious …, 2011 - biomedcentral.com... 21] using a commercial kit to test for 43 individual spacers (Isogen, IJsselstein, The ... Spoligotyping provided a relatively simple and rapid means of describing the population of ... improved discrimination of strains sharing the CAS1_Dehli and Beijing spoligotypes when using MIRU ...Related articles - Cached - All 11 versions - Import into BibTeX

[PDF] MOLECULAR CHARACTERISATION OF MYCOBACTERIUM BOVIS ISOLATED FROM AFRICAN BUFFALOES (SYNCERUS CAFFER) IN HLUHLUWE-IMFOLOZI PARK IN KWAZULU-NATAL, SOUTH AFRICA [PDF] from scielo.org.za TM Hlokwe, AO Jenkins, EM Streicher… - … Journal of Veterinary …, 2011 - scielo.org.za... One of the observed spoligotypes (SB0130) was dominant and represented 75% of isolates ...Spoligotyping Spoligotyping was done according to a standardised international method(Kamerbeek et al. 1997) using a commercially available kit (Isogen Biosciences BV, Maarsen). ...Related articles - View as HTML - All 10 versions - Import into BibTeX

GENOTYPING DID NOT EVIDENCE ANY CONTRIBUTION OF MYCOBACTERIUM BOVIS TO HUMAN TUBERCULOSIS IN BRAZIL [PDF] from redetb.org A Rocha, AR Elias, LF Sobral, DF Soares… - Tuberculosis, 2011 - Elsevier... using PCR and hybridization conditions on a commercially available membrane (Isogen Biosciene BV ... All isolates that yielded spoligotypes that were recognized as not previously described patterns were submitted to at least one additional spoligotyping assay and ...Related articles - All 6 versions - Import into BibTeX

MOLECULAR AND EPIDEMIOLOGICAL CHARACTERIZATION OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS ISOLATES IN JOHANNESBURG, SOUTH AFRICA [PDF] from wits.ac.zaCK Mlambo - 2011 - wiredspace.wits.ac.za ... 61 Table 3.6 Description of the orphan MIRU patterns (n=45) from this study and corresponding spoligotyping-based information (octal code, SIT, and clade)…………64 Table 3.7 MIRU profiles of the four newly created spoligotypes (10 ...Related articles - Import into BibTeX

USE OF SPOLIGOTYPE ANALYSIS TO DETECT LABORATORY CROSS-CONTAMINATIONB Nivin, J Driscoll, T Glaser, P Bifani… - Infection control and …, 2000 - JSTOR ... The spoligotyping analysis was performed using a commercially available kit (Isogen Bioscience BV ... In several cases of identical spoligotypes, the Wadsworth Center indicated that the ...Spoligotyping is an effective, relatively inexpensive (approximately 10% the cost of RFLP ...Cited by 12 - Related articles - BL Direct - All 6 versions - Import into BibTeX

CLINICAL AND GENOTYPIC CHARACTERISTICS OF CHILDHOOD TUBERCULOSIS IN CHONGQING, CHINA L Xing, R Liu, Q Li, Z Peng… - European Journal of Clinical …, 2011 - Springer... Of 105 isolates spoligotyped, the major spoligotypes found were ... Spoligotyping was performed according to the internation- ally standardized protocol [5]. A commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as described ...Related articles - All 2 versions - Import into BibTeX

TUBERCULOSIS DUE TO MYCOBACTERIUM BOVIS AND MYCOBACTERIUM CAPRAE IN SHEEPM Muņoz Mendoza, L Juan, S Menéndez… - The Veterinary …, 2011 - Elsevier

... by hybridisation of the biotin-labelled PCR product onto spoligotyping membrane (Isogen Bioscience BV). ... Isolates were characterised by spoligotyping as M. caprae SB0157 (flock A) and M. bovis ... close contact with cattle or goats infected by the same spoligotypes (Table 1 ...Cited by 3 - Related articles - All 2 versions - Import into BibTeX

[HTML] MYCOBACTERIUM BOVIS INFECTION IN GOATS FROM THE NORTHEAST REGION OF BRAZIL [HTML] from scielo.br SSS Higino, SR Pinheiro, GO Souza… - Brazilian Journal of …, 2011 - SciELO Brasil... Isolate was characterised by spoligotyping as described (7). The amplified product was detected by hybridization of the biotin-labelled PCR product onto spoligotyping membrane (Isogen Bioscience BV, Maarssen, The Netherlands). ...

EPIDEMIOLOGICAL STUDIES OF BEIJING STRAINS OF MYCOBACTERIUM TUBERCULOSIS FROM TAIPEI AND OTHER ASIAN CITIES BASED ON MIRU PROFILES JUNREN SUN, HYUN DOU, SYI LEE… - Apmis, 2011 - Wiley Online Library... on isolates from areas where the Beijing strain is prevalent as most isolates in this family share the same spoligotypes (1, 5 ... Spoligotyping ... to the manufacturers' protocol, to a set of 43 oligonucle-otides of DR spacers on a membrane using the Isogen Bioscience spoligotyping kit ...

 

 

 

 

Year 2012

 

 Diversity of Mycobacterium tuberculosis Isolates from New Pulmonary Tuberculosis Cases in Addis Ababa, Ethiopia.Adane Mihret, Yonas Bekele, Andre G. Loxton, Annemie M. Jordan, Lawrence Yamuah, Abraham Aseffa, Rawleigh Howe, Gerhard Walzl,Tuberc Res Treat. 2012; 2012: 892079.  Published online 2012 Nov 22. doi: 10.1155/2012/892079.PMCID: PMC3513727

 

 Plasma Level of IL-4 Differs in Patients Infected with Different Modern Lineages of M. tuberculosis.Adane Mihret, Yonas Bekele, Andre G. Loxton, Abraham Aseffa, Rawleigh Howe, Gerhard Walzl,J Trop Med. 2012; 2012: 518564.  Published online 2012 Sep 25. doi: 10.1155/2012/518564.PMCID: PMC3463165

 

 Modern lineages of Mycobacterium tuberculosis in Addis Ababa, Ethiopia: implications for the tuberculosis control programe.A Mihret, Y Bekele, M Aytenew, Y Assefa, M Abebe, L Wassie, GA Loxton, L Yamuah, A Aseffa, G Walzl, R Howe.Afr Health Sci. 2012 Sep; 12(3): 339–344.  PMCID: PMC3557690

 

 Rapid Spoligotyping of Mycobacterium tuberculosis Complex Bacteria by Use of a Microarray System with Automatic Data Processing and Assignment.Anke Ruettger, Johanna Nieter, Artem Skrypnyk, Ines Engelmann, Albrecht Ziegler, Irmgard Moser, Stefan Monecke, Ralf Ehricht, Konrad Sachse

J Clin Microbiol. 2012 Jul; 50(7): 2492–2495.  doi: 10.1128/JCM.00442-12

  Whole cell & culture filtrate proteins from prevalent genotypes of Mycobacterium tuberculosis provoke better antibody & T cell response than laboratory strain H37Rv.Gavish Kumar, Hari Shankar, Mamta Chahar, Pragya Sharma, Virendra Singh Yadav, Devendra Singh.Chauhan, Vishwa Mohan Katoch, Beenu Joshi

Indian J Med Res. 2012 May; 135(5): 745–755. PMCID: PMC3401709

 

 Rapid Test for Identification of a Highly Transmissible Mycobacterium tuberculosis Beijing Strain of Sub-Saharan Origin.María Isabel Millán-Lou, Henar Alonso, Patricia Gavín, Melissa Hernández-Febles, María Isolina

Campos-Herrero, Rodolfo Copado, Fernando Cañas, Kristin Kremer, José Antonio Caminero, Carlos  Martín, Sofía Samper.J Clin Microbiol. 2012 Feb; 50(2): 516–518.  doi: 10.1128/JCM.06314-11.PMCID: PMC3264144

 

2011

High prevalence of subclinical tuberculosis in HIV-1-infected persons without advanced  immunodeficiency: implications for TB screening.Tolu Oni, Rachael Burke, Relebohile Tsekela, Nonzwakazi Bangani, Ronnett Seldon, Hannah P Gideon, Kathryn Wood, Katalin A Wilkinson, Tom H M Ottenhoff, Robert J Wilkinson.Thorax. 2011 Aug; 66(8): 669–673.  Published online 2011 May 31. doi: 10.1136/thx.2011.160168

PMCID: PMC3142344

 

The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines for Quality Assurance when working on membranes

Edgar Abadia, Jian Zhang, Viviana Ritacco, Kristin Kremer, Raymond Ruimy, Leen Rigouts, Harrison Magdinier Gomes, Atiná Ribeiro Elias, Maryse Fauville-Dufaux, Karolien Stoffels, Voahangy Rasolofo-Razanamparany, Darío Garcia de Viedma, Marta Herranz, Sahal Al-Hajoj, Nalin Rastogi, Carlo Garzelli, Enrico Tortoli, Philip N Suffys, Dick van Soolingen, Guislaine Refrégier, Christophe Sola.BMC Infect Dis. 2011; 11: 110.  Published online 2011 Apr 28. doi: 10.1186/1471-2334-11-110.PMCID: PMC3107175

 

Bovine Tuberculosis in a Nebraska Herd of Farmed Elk and Fallow Deer: A Failure of the Tuberculin Skin Test and Opportunities for Serodiagnosis.W. Ray Waters, Gary E. Stevens, Mark A. Schoenbaum, Kathy A. Orloski, Suelee Robbe-Austerman, N. Beth Harris, S. Mark Hall, Bruce V. Thomsen, Arach J. Wilson, Roger E. Brannian, Jeffrey T. Nelson, Shawn Schafer, Javan Esfandiari, Meghan Dutton, Rena Greenwald, Konstantin P. Lyashchenko.Vet Med Int. 2011; 2011: 953985.  Published online 2011 Apr 14. doi: 10.4061/2011/953985

PMCID: PMC3087612

 

Pathology of Camel Tuberculosis and Molecular Characterization of Its Causative Agents in astoral Regions of Ethiopia.Gezahegne Mamo, Gizachew Bayleyegn, Tesfaye Sisay Tessema, Mengistu Legesse, Girmay Medhin, Gunnar,Bjune, Fekadu Abebe, Gobena Ameni.PLoS One. 2011; 6(1): e15862.  Published online 2011 Jan 24. doi: 10.1371/journal.pone.0015862.PMCID: PMC3025912

[HTML] Bovine tuberculosis in a Nebraska herd of farmed elk and fallow deer: a failure of the tuberculin skin test and opportunities for serodiagnosis

[HTML] from hindawi.comW Waters, GE Stevens, MA Schoenbaum… - Veterinary medicine …, 2011 - hindawi.com... Isolates of M. bovis were identified by a combination of Ziehl-Neelsen acid-fast staining, nucleicacid probes (AccuProbe, Gen-Probe, San Diego, CA, USA), and spoligotyping (OcimumBiosolutions Ltd., Hyderabad, India). Identification of atypical Mycobacteria spp. ...Cited by 2 - Related articles - Cached - All 13 versions - Import into BibTeX

 [HTML] The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines …E Abadia, J Zhang, V Ritacco… - BMC infectious …, 2011 - biomedcentral.com... of hard to interpret spoligotypes and samples representative of the work flow, so we could evaluatethe benefits of a new platform regarding the classical one on these samples. Most centers hadperformed spoligotyping using commercial membranes (Ocimum, Hyderabad, India ...Cited by 3 - Related articles - Cached - All 26 versions - Import into BibTeX

Quality assurance for molecular epidemiology of tuberculosis methods in the mycobacterium reference laboratoryPJ Freidlin, D Goldblatt, H Kaidar-Shwartz… - … and Quality Assurance: …, 2011 - Springer... for spoli- gotyping results, standardization and reproducibility depend on the quality of thecommercial spoligotyping kit (Ocimum). ... Spoligotyping Pattern ... Both PETRA (a MIRU-VNTR lineagewith central asian spoligotypes, CAS) and Beijing clusters have been observed in other ...Related articles - All 3 versions - Import into BibTeX

DETERMINATION OF DRUG SUSCEPTIBILITY PATTERNS AND GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM KANPUR DISTRICT; …S Purwar, S Chaudhari, VM Katoch… - Infection, Genetics and …, 2011 - Elsevier... commercially available kit 126 (Isogen Biosciences , BV, Maarsen, The Netherlands now OcimumBiosolutions) and ... Owing to small sample size, the exact prevalence of various 272 spoligotypesand M ... Out of the isolates clustered by spoligotyping, all 280 were unique strains by ...Cited by 3 - Related articles - All 4 versions - Import into BibTeX

New insight into the molecular characterization of isoniazid and rifampicin resistant< i> Mycobacterium tuberculosis</i> strains from Saudi ArabiaB Varghese, A Hillemann, DR Wijayanti… - Infection, Genetics and …, 2012 - Elsevier... Of the 151 isolates, 146 with interpretable mutation results were subjected to MIRU-VNTR typingand spoligotyping. Spoligotyping was performed as previously described (Kamerbeek et al., 1997)with the commercial kit (Ocimum Biosolutions, Hyderabad, India). ...All 2 versions - Import into BibTeX

High prevalence of subclinical tuberculosis in HIV-1-infected persons without advanced immunodeficiency: implications for TB screeningT Oni, R Burke, R Tsekela, N Bangani, R Seldon… - Thorax, 2011 - thorax.highwire.org... Lowenstein–Jensen slope cultures. The DNA was used to perform spoligotypingas previously described4 using a commercially available kit from Ocimum BiosolutionsBV, The Netherlands. The hybridisation patterns obtained ...Cited by 1 - Related articles - All 12 versions - Import into BibTeX

[HTML] Pathology of camel tuberculosis and molecular characterization of its causative agents in pastoral regions of Ethiopia

G Mamo, G Bayleyegn, TS Tessema, M Legesse… - PloS one, 2011 - dx.plos.org... Spoligotyping. Spoligotyping was performed as previously described by Kamerbeekand coauthors [22] and according to the spoligotype kit supplier's instructions (OcimumBiosolutions Company, Iisselstein, The Netherlands). ...Cited by 3 - Related articles - Cached - All 9 versions - Import into BibTeX

POPULATION-BASED INVESTIGATION OF FLUOROQUINOLONES RESISTANT TUBERCULOSIS IN RURAL EASTERN CHINA

Y Hu, B Mathema, W Wang, B Kreiswirth, W Jiang… - Tuberculosis, 2011 - Elsevier

... Spoligotyping was carried out using commercial kits from Isogen Bioscience BV (Maarssen, The Netherlands). 15 Strain family was determined by comparison of spoligotyping patterns with the SpoligDB4 database and assigned a spoligotyping international type (SIT). ...Cited by 2 - Related articles - All 4 versions - Import into BibTeX

[HTML] THE GUINEA-BISSAU FAMILY OF MYCOBACTERIUM TUBERCULOSIS COMPLEX REVISITED

R Groenheit, S Ghebremichael, J Svensson… - PLoS One, 2011 - dx.plos.org

... All isolates that were subjected to RFLP were also spoligotyped. ... Altogether, 414 isolates were available for spoligotyping, of which 336 isolates were grouped into 42 clusters ... Out of 229 isolates, 82 different spoligotypes were obtained, of which 168 (73.4%) were clustered into ...Cited by 4 - Related articles - Cached - All 5 versions - Import into BibTeX

GENETIC DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS IN MBARARA, SOUTH WESTERN UGANDA

J Bazira, M Matte, BB Asiimwe… - African health sciences, 2011 - ajol.info

... was performed as previously described29 using a commercially available kit (Isogen Bioscience BV ... Spoligotypes that did not match any existing pattern in the SpolDB4 database were ... tuberculosis within the Mbarara, we have used RD analysis and spoligotyping to characterize ...Related articles - All 8 versions - Import into BibTeX

FLUOROQUINOLONE RESISTANCE AMONG MYCOBACTERIUM TUBERCULOSIS STRAINS FROM KARACHI, PAKISTAN: DATA FROM COMMUNITY-BASED FIELD CLINICS

Y Rafiq, K Jabeen, R Hasan, S Jafri… - Antimicrobial agents …, 2011 - Am Soc Microbiol... was performed on all FQ-resistant strains using a commercially available kit provided by Isogen Life Science BV ... The spoligotypes were compared with the most prevalent M. tuberculosis subfamilies, as identified by the world spoligotyping database, SpolDB4.0, of ...Related articles - All 6 versions - Import into BibTeX

[HTML] CHARACTERISTICS OF PNCA MUTATIONS IN MULTIDRUG-RESISTANT TUBERCULOSIS IN TAIWAN

YC Chiu, SF Huang, KW Yu, YC Lee… - BMC infectious …, 2011 - biomedcentral.com

... Genotyping. All clinical isolates were genotyped by a commercial spoligotyping kit (Isogen Bioscience BV, Maarssen, Netherlands). The "Beijing strain" was defined as deletion from spacer 1 to spacer 34 in the direct repeat region and the presence of (at least 3) spacers 35-43. ...Related articles - Cached - All 11 versions - Import into BibTeX

[HTML] INITIAL PRESENTATIONS PREDICT MORTALITY IN PULMONARY TUBERCULOSIS PATIENTS-A PROSPECTIVE OBSERVATIONAL STUDY

 

JY Feng, WJ Su, YC Chiu, SF Huang, YY Lin… - PloS one, 2011 - dx.plos.org

... All clinical isolates were genotyped using a commercial spoligotyping kit (Isogen Bioscience BV, Maarssen, Netherlands). The “Beijing strain” was defined as a deletion from spacer 1 to spacer 34 in the direct repeat region and the presence of (at least 3) spacers in 35–43. ...Related articles - Cached - All 4 versions - Import into BibTeX

[HTML] MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS, BUENOS AIRES, ARGENTINA

X Gonzalo, M Ambroggi, E Cordova… - Emerging Infectious …, 2011 - ncbi.nlm.nih.gov

... DNA was extracted from cultures at this UK laboratory; spoligotyping was performed according to the manufacturer's instructions (Isogen Life Science, IJsselstein, the Netherlands); and data were analyzed with SPOTCLUST (http://cgi2.cs.rpi.edu/~bennek/SPOTCLUST.html). ...Related articles - All 4 versions - Import into BibTeX

CHARACTERISTICS OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS IN TAIWAN: A POPULATION-BASED STUDY

CW Chang, MH Wu, PC Chuang… - Infection, Genetics and Evolution, 2011 - Elsevier

... 12 13 Spoligotyping 14 A commercially available kit (Isogen Bioscience BV, Maarssen, The 15 Netherlands) was used as ... Even through spoligotyping results 3 revealed the genetic diversities of MDR M. tuberculosis isolates in Taiwan, 4 identical spoligotypes could not ...Cited by 2 - Related articles - All 2 versions - Import into BibTeX

MOLECULAR PATTERN OF MYCOBACTERIUM BOVIS ISOLATES AND ITS RELATIONSHIP WITH RISK FACTORS ASSOCIATED WITH THE PRESENCE OF BOVINE …

IF Padilla-Campos, JC Segura-Correa… - Tropical and …, 2011 - redalyc.uaemex.mx

... Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors. ... Spoligotyping was carried out following the protocol described by Kamerbeek et al ... sequences from M. tuberculosis and six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The ...Related articles - All 2 versions - Import into BibTeX

[HTML] FINE-TUNING THE SPACE, TIME, AND HOST DISTRIBUTION OF MYCOBACTERIA IN WILDLIFE

C Gortazar, MJ Torres, P Acevedo, J Aznar… - BMC …, 2011 - biomedcentral.com

... Typing. The M. bovis isolates were further characterized by spoligotyping [40]. The amplified product was detected by hybridization of the biotin-labelled PCR product onto spoligotyping membrane (Isogen Bioscience BV, Maarssen, The Netherlands). ...Cited by 6 - Related articles - Cached - All 19 versions - Import into BibTeX

MOLECULAR PATTERN OF MYCOBACTERIUM BOVIS ISOLATES AND ITS RELATIONSHIP WITH RISK FACTORS ASSOCIATED WITH THE PRESENCE OF …

IF Padilla, JC Segura, JC Rodriguez… - Tropical and …, 2011 - ccba.uady.mx

... Key words: Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors. ... Spoligotyping was carried out following the protocol described by Kamerbeek et al ... sequences from M. tuberculosis and six spacer sequences for M. bovis BCG were covalently bound (ISOGEN, Maarssen, The ...Related articles - All 2 versions - Import into BibTeX

EPIDEMIOLOGICAL TRENDS AND CLINICAL COMPARISONS OF< I> MYCOBACTERIUM</I>< I> TUBERCULOSIS</I> LINEAGES IN THAI TB MENINGITIS

K Faksri, F Drobniewski, V Nikolayevskyy, T Brown… - Tuberculosis, 2011 - Elsevier

... isolated from CSF samples collected from 1996 to 2004 with IS6110 RFLPs (120 RFLP patterns were available) and spoligotyping results (141 spoligotypes were available ... 27 Spoligotyping was performed according to the manufacturer's instructions (Isogen Bioscience BV ...Cited by 1 - Related articles - All 3 versions - Import into BibTeX

A NOVEL METHOD FOR THE RAPID AND PROSPECTIVE IDENTIFICATION OF BEIJING MYCOBACTERIUM TUBERCULOSIS STRAINS BY HIGH‐RESOLUTION MELTING ANALYSIS

M Alonso, Y Navarro, F Barletta… - Clinical …, 2011 - Wiley Online Library

... Thirty-two MTB strains (18 representatives of the Beijing genotype and 14 representatives of the non-Beijing genotype, according to their spoligotypes) were grown on ... Spoligotyping was performed in accordance with the manufacturer's instructions (Isogen, De Meern, the ...Cited by 3 - Related articles - All 3 versions - Import into BibTeX

QUALITY ASSURANCE FOR MOLECULAR EPIDEMIOLOGY OF TUBERCULOSIS METHODS IN THE MYCOBACTERIUM REFERENCE LABORATORY

PJ Freidlin, D Goldblatt, H Kaidar-Shwartz… - … and Quality Assurance: …, 2011 - Springer

... 43 spacer spoligotyping was performed according to the manufacturer's instructions (Isogen/Ocimun) as described previously. ... 27 Spoligotyping Pattern ... Both PETRA (a MIRU-VNTR lineage with central asian spoligotypes, CAS) and Beijing clusters have been observed in other ...Related articles - All 3 versions - Import into BibTeX

EPIDEMIOLOGICAL ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED IN LODZ, POLAND

M Krawczyk, A Brzostek, A Gorna… - … of Tuberculosis and …, 2011 - ingentaconnect.com

... of van Embden et al.16 Spoligotyping was performed as described by Kame rbeek et al., using the Isogen Life Science kit ... Spoligotyping analysis showed that 184 (78.6%) of the strains were grouped in clusters and 50 (21.4%) strains possessed unique spoligotypes. ...Cited by 1 - Related articles - All 3 versions - Import into BibTeX

ONE YEAR NATIONWIDE EVALUATION OF 24-LOCUS MIRU-VNTR GENOTYPING ON SLOVENIAN< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES

U Bidovec-Stojkovic, M Zolnir-Dovc… - Respiratory Medicine, 2011 - Elsevier

... 4,9,20 Spoligotyping was performed using a commercially available kit (Isogen Bioscience BV ... identically defined by IS6110 RFLP and 24-locus MIRU-VNTR typing combined with spoligotyping. ...jointly defined by identical 24-locus MIRU-VNTR types and spoligotypes were only ...Related articles - All 3 versions - Import into BibTeX

MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX (MTBC) ISOLATED FROM CATTLE IN NORTHEAST AND NORTHWEST CHINA

Y Du, Y Qi, L Yu, J Lin, S Liu, H Ni… - Research in veterinary …, 2011 - Elsevier

... 2.5. Spoligotyping. Spoligotyping kits and Mini-blotter MN45 were obtained from Isogen Life Science Co. (Utrecht, the Netherlands). The Streptavidin-ß-peroxidase conjugate was produced by Roche Applied Science Co. (Penzberg, Germany). ...Cited by 3 - Related articles - All 3 versions - Import into BibTeX

[HTML] TRANSMISSION PATTERN OF DRUG-RESISTANT TUBERCULOSIS AND ITS IMPLICATION FOR TUBERCULOSIS CONTROL IN EASTERN RURAL CHINA

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Y Hu, B Mathema, W Jiang, B Kreiswirth, W Wang… - PloS one, 2011 - dx.plos.org

... Hybridized DNA was detected using an enhanced chemiluminescene kit from Isogen Bioscience BV ... b Probaiblity that the Spoligotyping pattern belongs to the clades. ... N, Bennett KP (2006) Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. ...Related articles - Cached - All 7 versions - Import into BibTeX

SUBLINEAGES OF BEIJING STRAIN OF MYCOBACTERIUM TUBERCULOSIS IN SRI LANKA

US Rajapaksa… - Indian Journal of Microbiology, 2011 - Springer

... Spoligotyping was carried out according to manufacturer's guidelines using a commercially available kit (Isogen, Maarsen MV, The Netherland). Fourteen iso-lates that confirmed to be Beijing strain were further characterised. ...Related articles - All 2 versions - Import into BibTeX

DETERMINATION OF DRUG SUSCEPTIBILITY PATTERNS AND GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM KANPUR DISTRICT; …

S Purwar, S Chaudhari, VM Katoch… - Infection, Genetics and …, 2011 - Elsevier

... 43 spacers was done with the help of a commercially available kit 126 (Isogen Biosciences , BV ... Owing to small sample size, the exact prevalence of various 272 spoligotypes and M.tuberculosis ... Out of the isolates clustered by spoligotyping, all 280 were unique strains by IS6110 ...Cited by 3 - Related articles - All 4 versions - Import into BibTeX

MOLECULAR EPIDEMIOLOGY OF HUMAN AND ANIMAL TUBERCULOSIS IN IBADAN, SOUTHWESTERN NIGERIA

AO Jenkins, SIB Cadmus, EH Venter… - Veterinary …, 2011 - Elsevier

... Spoligotyping167 Spoligotyping confirmed the species identification of 19 M. tuberculosis and 13 M ... None of these CAM 173 variant spoligotypes were found in the spolDB4 database and were ...All M. tuberculosis strains 175 from PTB patients that were spoligotyped had identical ...Cited by 1 - Related articles - All 5 versions - Import into BibTeX

GENETIC DIVERSITY OF THE MYCOBACTERIUM TUBERCULOSIS BEIJING FAMILY BASED ON IS6110, SNP, LSP AND VNTR PROFILES FROM THAILAND

K Faksri, F Drobniewski, V Nikolayevskyy… - Infection, Genetics and …, 2011 - Elsevier

... Eighty MTB Beijing isolates from CSF samples with IS6110 RFLPs (69 RFLP patterns were available) and spoligotyping results (all 80 spoligotypes were available ... Spoligotyping wasperformed according to the manufacturer s instructions (Isogen Bioscience BV ...Cited by 2 - Related articles - All 3 versions - Import into BibTeX

[PDF] 骨结核临床分离菌株基因分型的特点

李文胜, 刘毅, 孙勇, 田苗, 刘忠泉… - 中华临床医师杂志 ( …, 2011 - clinicmed.net

... TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自TaKaRa 公司。凝胶成像仪购自FOTO DYNE 公司。 Spoligotyping 试 剂盒以及Miniblotter MN45为荷兰Isogen Bioscience 公司产品。 链霉亲和素为Sigam 公司产品。 化学发光 ...Related articles - View as HTML - All 3 versions - Import into BibTeX

MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS IN THE UNITED ARAB EMIRATES

A Saedi… - 2011 - qmro.qmul.ac.uk

... 130 3.2.11 Identification of the UAE isolates by spoligotyping..... 132 3.2.12 Comparison between MIRU-typed and spoligotyped families in association with katG463/gyrA95 groupings ..... ...Related articles - All 2 versions - Import into BibTeX

[HTML] MYCOBACTERIUM BOVIS IN SWINE: SPOLIGOTYPING OF ISOLATES FROM ARGENTINA

S Barandiaran, EV Moras, A Adri& - Veterinary Medicine …, 2011 - hindawi.com

... Spoligotyping [14] was carried out by using the spoligotyping kit (Isogen Biosolutions BV, Ocimun ...Guerin (BCG) (ATCC 27289) were included as reference strains in each spoligotyping experiment ...isolates were defined as two or more M. bovis strains with identical spoligotypes. ...Cited by 1 - Related articles - Cached - All 11 versions - Import into BibTeX

[HTML] SPOLIGOTYPING OF CLINICAL MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM THE STATE OF MINAS GERAIS, BRAZIL

SS Miranda, WS Carvalho, PN Suffys… - Memórias do Instituto …, 2011 - SciELO Brasil

... was resuspended in the same TE buffer, stored at -20ºC and subjected to spoligotyping using a previously published protocol (Kamerbeek et al. 1997) and a commercially available kit (Isogen Life Science BV, Utrecht, The Netherlands). The hybridised spoligotypes in binary ...Related articles - Cached - All 7 versions - Import into BibTeX

EVALUATION OF SPOLIGOTYPING AND MIRU-VNTR FOR MYCOBACTERIUM BOVIS IN XINJIANG, CHINA

Z Sun, R Cao, M Tian, X Zhang, X Zhang… - Research in Veterinary …, 2011 - Elsevier

... Additionally, two new spoligotypes (SB1903 and SB1904) and special repeat numbers of 3 loci(ETR ... Spoligotyping was firstly analyzed and interpreted in octal codes (Dale et al., 2001) following the instruction (http://www.mbovis.org) with a commercial kit (Isogen Bioscience BV ...Cited by 2 - Related articles - All 2 versions - Import into BibTeX

[HTML] MYCOBACTERIUM TUBERCULOSIS SPOLIGOTYPES AND DRUG SUSCEPTIBILITY PATTERN OF ISOLATES FROM TUBERCULOSIS PATIENTS IN SOUTH-WESTERN UGANDA

J Bazira, BB Asiimwe, ML Joloba… - BMC infectious …, 2011 - biomedcentral.com

... Spoligotyping. Standard spoligotyping was performed as previously described [29] using a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands). ... Spoligotyping. All the isolates had spoligotypes characteristic of M. tuberculosis. ...Cited by 1 - Related articles - Cached - All 12 versions - Import into BibTeX

A FIRST INSIGHT ON THE POPULATION STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS COMPLEX AS STUDIED BYSPOLIGOTYPING AND MIRU-VNTRS IN BOGOTÁ, COLOMBIA

I Cerezo, Y Jiménez, J Hernandez, T Zozio… - Infection, Genetics and …, 2011 - Elsevier

... Kamerbeek et al., 1997) according to the manufacturer s instructions (Isogen Bioscience, the … Spoligotype International Type (SIT) and MIRU International Type (MIT) designate spoligotypes and MIRU ... was used to build minimum spanning trees based on spoligotyping and MIRU ...All 2 versions - Import into BibTeX

[PDF] PRELIMINARY MAPPING STUDY OF MYCOBACTERIUM TUBERCULOSIS' COMPLEX DNA BYSPOLIGOTYPING ON ISOLATED SPUTUM OF TUBERCULOSIS PATIENT …

[PDF] from digitaljournals.org

V Lisdawati, I Parwati… - Bulletin of Health …, 2011 - indonesia.digitaljournals.org

... PCR, denaturasi menggunakan thermocycler selama 10 menit pada suhu 99,9°C. Kemudian pada mini blotter MN45 yang telah diletakkan membran spoligotyping kit (IM9702, Isogen Biosolution, BV) berlabel 43 DNA spacer Mtb complex segera hibridisasikan DNA hasil PCR. ...Related articles - View as HTML - Import into BibTeX

[PDF] INFECTION OF EURASIAN BADGERS (MELES MELES) WITH MYCOBACTERIUM BOVIS

A Balseiro, O Rodríguez, P González-Quirós… - prevalence, 2011 - uclm.es

... al., 2009). 125 MTC isolates were characterised hybridisation of biotin-labelled PCR products 126 onto a spoligotyping membrane (Isogen Bioscience BV), followed by spoligotyping 127 (Kamerbeek et al., 1997). Results were ...Cited by 2 - Related articles - View as HTML - All 2 versions - Import into BibTeX

CLINICAL AND MICROBIOLOGICAL CHARACTERISTICS OF URINE CULTURE-CONFIRMED GENITOURINARY TUBERCULOSIS AT MEDICAL CENTERS IN TAIWAN FROM 1995 TO 2007

HL Hsu, CC Lai, MC Yu, FL Yu, JC Lee… - European journal of …, 2011 - Springer

... commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as described by the manufacturer. The fourth international spoligotyping database (SpolDB4) database was used to assign genotype families to the spoligotypes obtained [14 ...Related articles - All 5 versions - Import into BibTeX

MOLECULAR TYPING OF MYCOBACTERIUM TUBERCULOSIS CIRCULATED IN MOSCOW, RUSSIAN FEDERATION

MV Afanas' ev, LN Ikryannikova, EN Il'ina… - European Journal of …, 2011 - Springer

... spacers probes from the spoligotyping kit from Isogen Lifescience (cat. no. IM9701). 182 Eur J Clin Microbiol Infect Dis (2011) 30:181–191 Page 3. Spoligotypes in binary format were entered in an Excel spreadsheet and compared with the spoligotyping database SpolDB4 [13]. ...Cited by 7 - Related articles - All 4 versions - Import into BibTeX

ROLE OF 2-MONTH SPUTUM SMEARS IN PREDICTING CULTURE CONVERSION IN PULMONARY TUBERCULOSIS

WJ Su, JY Feng, YC Chiu… - European Respiratory …, 2011 - Eur Respiratory Soc

... All clinical isolates were genotyped by commercial spoligotyping kit (Isogen Bioscience BV, Maarssen, the Netherlands). The “Beijing strain” was defined as deletion from spacer 1 to spacer 34 in the direct repeat region and the presence of (at least three of) spacers 35–43. ...Cited by 5 - Related articles - All 6 versions - Import into BibTeX

[PDF] A TWO-STEP STRATEGY FOR MOLECULAR TYPING OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATES FROM POLAND.

T JAGIELSKI, EWAA KOPEĆ… - Polish journal of …, 2011 - pjm.microbiology.pl

... Spoligotyping was perfor- med with a commercially available kit (Isogen Bio- science BV ... e isolates whose spoligotype patterns were already recorded in the database, were assigned “shared types”,

whereas those of spoligotypes identified for the first time, were ...View as HTML - All 3 versions - Import into BibTeX

MOLECULAR TECHNOLOGIES USED IN DETECTING OF SENSITIVE AND ISONIAZID-RESISTANT MYCOBACTERIUM TUBERCULOSIS

OY Limanskaya… - Cytology and Genetics, 2011 - Springer

... The PCR was carried out by a dry kit of reagents for DNA amplification (Isogen, Russian Federation) in 20–50 μl of reaction mixture containing 4 mM NaCl, 50 mM KCl, 12 mM Tris HCl (pH 8.0), 2.5 mM MgCl2, 200 μM dNTPs, 0.1–0.5 μM of each primer, and 1 unit of Taq DNA ...Related articles - All 4 versions - Import into BibTeX

STREPTOMYCIN RESISTANCE AND LINEAGE-SPECIFIC POLYMORPHISMS IN MYCOBACTERIUM TUBERCULOSIS GIDB GENE

FS Spies, AW Ribeiro, DF Ramos… - Journal of clinical …, 2011 - Am Soc Microbiol

... Genotyping.Spoligotyping was performed using a commercial kit (Isogen Biosciences BV, Netherlands ... it was classified as LAM3 and S/convergent sublineage by spoligotyping but as non ...Spoligotypes evolve through successive loss of spacer DNA sequences (16), and since ...

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GENOTYPING AND DRUG RESISTANCE PATTERNS OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN FIVE PROVINCES OF CHINA

YL Guo, Y Liu, SM Wang, CY Li… - … of Tuberculosis and …, 2011 - ingentaconnect.com

... Of the (Isogen Bioscience BV, De Meern, The Netherlands). ... 1–1800 = shared spoligotypes. Table 3 Results of MIRU typing and comparison of the three typing methods Isolate MIRU locus number IS6110-RFLP Cluster 2 4 10 16 20 23 24 26 27 31 39 40 Spoligotyping MIRU ...Cited by 3 - Related articles - All 3 versions - Import into BibTeX

ASSOCIATION OF< I> MYCOBACTERIUM TUBERCULOSIS</I> GENOTYPES AND CLINICAL AND EPIDEMIOLOGICAL FEATURES-A MULTI-CENTER STUDY IN TAIWAN

SF Huang, WJ Su, HY Dou, JY Feng… - Infection, Genetics and …, 2011 - Elsevier

... Spoligotyping was performed according to the method reported by Kamerbeek et al. (Kamerbeek et al., 1997) using a commercial kit (Isogen Biosolutions, Netherlands). The spoligotypes were identified according to the database SpoDB4.0 (Brudey et al., 2006), and the SIT ...Related articles - All 5 versions - Import into BibTeX

THE GENOTYPE CHARACTERISTIC OF CLINICAL ISOLATED STRAINS GETTING FROM BONE TUBERCULOSIS

LI Wen-sheng, LIU Yi, SUN Yong… - Chinese Journal of …, 2011 - global.clinicmed.net

... TM 琼脂糖购自赛百盛基因技 术有限公司。 DNA Ladder Marker 购自 TaKaRa 公司。凝胶成像仪购自 FOTO DYNE 公司。 Spoligotyping 试 剂盒以及 Miniblotter MN45为荷兰Isogen Bioscience 公司产品。 链霉亲和素为 Sigam 公司产品。 化学发光 ...Related articles - Import into BibTeX

MULTIPLE STRAINS OF< I> MYCOBACTERIUM BOVIS</I> REVEALED BY MOLECULAR TYPING IN A HERD OF CATTLE

EES Figueiredo, DF Ramos, L Medeiros… - The Veterinary …, 2011 - Elsevier

... to the spoligo-membrane was performed according to the manufacturer's instructions (Isogen

Life Sciences). ... The strains clustered by spoligotyping were analysed by VNTR-PCR to confirm

clonal relationships ... Four spoligotypes were identified among the 12 M. bovis isolates. ...

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DETERMINAÇÃO DO CUSTO BIOLÓGICO EM ISOLADOS DE MYCOBACTERIUM TUBERCULOSIS COM MUTAÇÕES NOS GENES RPSL, RRS, GIDB, RPOB E KATG

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FS Spies - 2011 - lume.ufrgs.br

... restriction fragment lenght polymorphism) da sequência de inserção IS6110, spoligotyping e

unidade de repetição de micobactéria- repetição em tandem de número variável (MIRU- ...

Spoligotyping é uma técnica de genotipagem que explora a variabilidade da região ...

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A MAJOR SUBGROUP OF BEIJING FAMILY MYCOBACTERIUM TUBERCULOSIS IS ASSOCIATED WITH MULTIDRUG RESISTANCE AND INCREASED TRANSMISSIBILITY

Y Hu, X Ma, EA Graviss, W Wang… - Epidemiology and …, 2011 - Cambridge Univ Press

... Spoligotyping was performed by using a com- mercial kit from Isogen Bioscience BV (Maarssen ...

0.04), and resistance to RIF and SM (P=0.04) (Table 1). Spoligotyping identified 243 ... Thirty-three

(9.4%) isolates had unique/novel spoligotypes, of which similarity analysis found that ...

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[HTML] MODERN AND ANCESTRAL GENOTYPES OF MYCOBACTERIUM TUBERCULOSIS FROM ANDHRA PRADESH, INDIA

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SK Thomas, CC Iravatham, BH Moni, A Kumar… - PloS one, 2011 - dx.plos.org

... the help of commercially available line probe arrays (Isogen Biosciences BV ... tuberculosis complex

genetic diversity: mining the fourth international spoligotyping database (SpolDB4 ... KP (2006)

Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. ...

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[HTML] TUBERCULOSIS IN SUDAN: A STUDY OF MYCOBACTERIUM TUBERCULOSIS STRAIN GENOTYPE AND SUSCEPTIBILITY TO ANTI-TUBERCULOSIS DRUGS

[HTML] from biomedcentral.com

GSS Eldin, I Fadl-Elmula, MS Ali… - BMC infectious …, 2011 - biomedcentral.com

... 21] using a commercial kit to test for 43 individual spacers (Isogen, IJsselstein, The ... Spoligotyping

provided a relatively simple and rapid means of describing the population of ... improved

discrimination of strains sharing the CAS1_Dehli and Beijing spoligotypes when using MIRU ...

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[PDF] MOLECULAR CHARACTERISATION OF MYCOBACTERIUM BOVIS ISOLATED FROM AFRICAN BUFFALOES (SYNCERUS CAFFER) IN HLUHLUWE-IMFOLOZI PARK IN KWAZULU-NATAL, SOUTH AFRICA

[PDF] from scielo.org.za

TM Hlokwe, AO Jenkins, EM Streicher… - … Journal of Veterinary …, 2011 - scielo.org.za

... One of the observed spoligotypes (SB0130) was dominant and represented 75% of isolates ...Spoligotyping Spoligotyping was done according to a standardised international method

(Kamerbeek et al. 1997) using a commercially available kit (Isogen Biosciences BV, Maarsen). ...

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GENOTYPING DID NOT EVIDENCE ANY CONTRIBUTION OF MYCOBACTERIUM BOVIS TO HUMAN TUBERCULOSIS IN BRAZIL

[PDF] from redetb.org

A Rocha, AR Elias, LF Sobral, DF Soares… - Tuberculosis, 2011 - Elsevier

... using PCR and hybridization conditions on a commercially available membrane (Isogen

Bioscience BV ... All isolates that yielded spoligotypes that were recognized as not previously

described patterns were submitted to at least one additional spoligotyping assay and ...

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MOLECULAR AND EPIDEMIOLOGICAL CHARACTERIZATION OF MULTIDRUG-RESISTANT MYCOBACTERIUM TUBERCULOSIS ISOLATES IN JOHANNESBURG, SOUTH AFRICA

[PDF] from wits.ac.za

CK Mlambo - 2011 - wiredspace.wits.ac.za

... 61 Table 3.6 Description of the orphan MIRU patterns (n=45) from this study and

corresponding spoligotyping-based information (octal code, SIT, and clade)…………

64 Table 3.7 MIRU profiles of the four newly created spoligotypes (10 ...

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USE OF SPOLIGOTYPE ANALYSIS TO DETECT LABORATORY CROSS-CONTAMINATION

B Nivin, J Driscoll, T Glaser, P Bifani… - Infection control and …, 2000 - JSTOR

... The spoligotyping analysis was performed using a commercially available kit (Isogen Bioscience

BV ... In several cases of identical spoligotypes, the Wadsworth Center indicated that the ...

Spoligotyping is an effective, relatively inexpensive (approximately 10% the cost of RFLP ...

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CLINICAL AND GENOTYPIC CHARACTERISTICS OF CHILDHOOD TUBERCULOSIS IN CHONGQING, CHINA

L Xing, R Liu, Q Li, Z Peng… - European Journal of Clinical …, 2011 - Springer

... Of 105 isolates spoligotyped, the major spoligotypes found were ... Spoligotyping was performed

according to the internation- ally standardized protocol [5]. A commercially available spoligotyping

kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as described ...

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TUBERCULOSIS DUE TO MYCOBACTERIUM BOVIS AND MYCOBACTERIUM CAPRAE IN SHEEP

M Muņoz Mendoza, L Juan, S Menéndez… - The Veterinary …, 2011 - Elsevier... by hybridisation of the biotin-labelled PCR product onto spoligotyping membrane (Isogen Bioscience BV). ... Isolates were characterised by spoligotyping as M. caprae SB0157 (flock A) and

M. bovis ... close contact with cattle or goats infected by the same spoligotypes (Table 1 ...

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[HTML] MYCOBACTERIUM BOVIS INFECTION IN GOATS FROM THE NORTHEAST REGION OF BRAZIL

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SSS Higino, SR Pinheiro, GO Souza… - Brazilian Journal of …, 2011 - SciELO Brasil

... Isolate was characterised by spoligotyping as described (7). The amplified product

was detected by hybridization of the biotin-labelled PCR product onto spoligotyping

membrane (Isogen Bioscience BV, Maarssen, The Netherlands). ...

 

EPIDEMIOLOGICAL STUDIES OF BEIJING STRAINS OF MYCOBACTERIUM TUBERCULOSIS FROM TAIPEI AND OTHER ASIAN CITIES BASED ON MIRU PROFILES

JUNREN SUN, HYUN DOU, SYI LEE… - Apmis, 2011 - Wiley Online Library

... on isolates from areas where the Beijing strain is prevalent as most isolates in this family share

the same spoligotypes (1, 5 ... Spoligotyping ... to the manufacturers' protocol, to a set of 43 oligonucle-

otides of DR spacers on a membrane using the Isogen Bioscience spoligotyping kit ...

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2010

Comparison of restriction fragment length polymorphism with the polymorphic guanine-cytosine-rich sequence and spoligotyping for differentiation of Mycobacterium …

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L Flores, LG Jarlsberg, EY Kim… - Journal of clinical …, 2010 - Am Soc Microbiol... 2004 was performed with standardized methods (15) using a membrane with 43 oligonucleotides representing the different direct variable repeats (DVRs) immobilized in specific locations (Ocimum Biosolutions, Inc., Gaithersburg, MD). Beginning in 2004, spoligotyping of the …

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Fitness study of the RDRio lineage and Latin American–Mediterranean family of Mycobacterium tuberculosis in the city of Rio Grande, Brazil

A Von Groll, A Martin, C Felix… - FEMS Immunology …, 2010 - Wiley Online Library... RD Rio BrgR: 5′-CACCGCCACGCTGAATGAGACCA-3′, –. Genotyping by spoligotyping. Spoligotyping was performed with a commercial kit (Ocimum Biosolutions BV, India) according to standard procedures (Kamerbeek et al., 1997). ...

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[HTML] Fitness of Mycobacterium tuberculosis strains of the W-Beijing and Non-W-Beijing genotype

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A Von Groll, A Martin, M Stehr, M Singh, F Portaels… - PloS one, 2010 - dx.plos.org... Strains were fully characterized and analyzed for the presence of mutations conferring resistance to RMP and INH. Spoligotyping was performed with a commercial kit (Ocimum Biosolutions BV, India) according to standard procedures [29]. Molecular characterization. ...

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[HTML] MOLECULAR DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM PATIENTS WITH PULMONARY TUBERCULOSIS IN MOZAMBIQUE

[HTML] from biomedcentral.com

SO Viegas, A Machado, R Groenheit… - BMC …, 2010 - biomedcentral.com ... using a commercially available kit (Isogen Life Science BV, Utrecht, The Netherlands). Spoligotyping results were analysed with the BioNumerics Software ver. 5.01 (Applied Maths, Kortrijk, Belgium). Database comparison and geographical distribution of spoligotypes. ...

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[PDF] ASSOCIATION AMONG THE RV2629 GENE, RPOB GENE, RFP RESISTANCE AND BEIJING GENOTYPE IN M. TUBERCULOSIS CLINICAL ISOLATES

[PDF] from academicjournals.org

J Zhang, X Wu, Y Yang, Y Zhuang… - African Journal of …, 2010 - academicjournals.org... The amplified PCR products were hybridized with spoligotyping Biodyne C membrane immobilized 43 oligonucleotide probes (Isogen Life Science, Holland). ... A total of 8 distinct spoligotyping patterns were observed. ... 7 isolates had unique spoligotypes (Figure 1, Tables 1 and 2 ...

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SINGLE NUCLEOTIDE POLYMORPHISMS IN CELL WALL BIOSYNTHESIS-ASSOCIATED GENES AND PHYLOGENY OF< I> MYCOBACTERIUM TUBERCULOSIS</I> LINEAGES

PC Chuang, YMA Chen, HY Chen… - Infection, Genetics and …, 2010 - Elsevier ... 2.2. Genotype analysis. Spoligotyping using a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands ... Spoligotypes and RFLP patterns were scanned and analyzed with Bionumerics ® software (version 4.601; Applied Maths, Kortrijk, Belgium). ...

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[HTML] ASSOCIATIONS BETWEEN MYCOBACTERIUM TUBERCULOSIS STRAINS AND PHENOTYPES

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T Brown, V Nikolayevskyy, P Velji… - Emerging Infectious …, 2010 - ncbi.nlm.nih.gov... Spoligotyping was performed according to the manufacturer's instructions (Isogen Lifescience, IJsselstein, the Netherlands) (9). Images were digitized and entered into BioNumerics software by using the BNIMA module (Applied Maths). Spoligotypes were assigned to families ...

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[HTML] SENSIBILIDAD Y ESPECIFICIDAD DE PCR ANIDADA Y SPOLIGOTYPING COMO PRUEBAS RÁPIDAS DE DIAGNÓSTICO DE TUBERCULOSIS BOVINA EN TEJIDO FRESCO

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F Milián Suazo, B Harris, C Arriaga Díaz… - Revista mexicana de …, 2010 - scielo.org.mx... Spoligotyping se realizó tal como lo describe Kamerbeek et al (17) . De manera resumida, la amplificación del ... por 30 seg. La hibridización se realizó de acuerdo a las recomendaciones del fabricante (Isogen). El ADN hibridado ...

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[HTML] GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM ALOW-ENDEMIC SETTING IN NORTHWESTERN STATE OF PARANÁ IN SOUTHERN BRAZIL

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EN Noguti, CQF Leite, AC Malaspina… - Memórias do Instituto …, 2010 - SciELO Brasil... Spoligotyping - Spoligotyping was performed in all 93 M. tuberculosis isolates, using the standardmethod ... of 43 spacer oligonucleotides covalently linked to the spoligo-membrane (Isogen Life Sciences ... Spoligotypes were reported using an octal code in which the 43-digit binary ...

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[HTML] EXTENSIVE TRANSMISSION OF ISONIAZID RESISTANT M. TUBERCULOSIS AND ITS ASSOCIATION WITH INCREASED MULTIDRUG-RESISTANT TB IN TWO RURAL COUNTIES OF EASTERN CHINA: A …

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Y Hu, S Hoffner, W Jiang… - BMC infectious …, 2010 - biomedcentral.com... Spoligotyping was carried out by using the commercial kit from Isogen Bioscience BV ... Table 3. Spoligotyping pattern and frequency of the MTB isolates included. ... B, Kurepina N, Bennett KP:Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. ...

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[HTML] MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM BOVIS ISOLATES FROM ETHIOPIAN CATTLE

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D Biffa, E Skjerve, J Oloya, A Bogale… - BMC veterinary …, 2010 - biomedcentral.com... spacers were then hybridized with the known oligonucleotide spacers ligated to the membrane(Isogen). ... The discriminatory power (HGDI) of the spoligotyping method was calculated to be 0.81. Also on the basis of the above findings (the number of distinct spoligotypes (S) = 12 ...

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[HTML] MYCOBACTERIUM TUBERCULOSIS BEIJING STRAIN, BAMAKO, MALI

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B Diarra, S Siddiqui, D Sogoba… - Emerging infectious …, 2010 - ncbi.nlm.nih.gov... See the article"Global Distribution of Mycobacterium tuberculosis Spoligotypes" in volume 8 on page 1347. ... Spoligotyping using a commercially available kit (Spoligotyping Isogen Life Science, De Meern, the Netherlands) showed characteristics of the Beijing clade (Appendix ...

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EVALUATION OF 24 LOCUS MIRU-VNTR GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS ISOLATES IN CANADA

S Christianson, J Wolfe, P Orr, J Karlowsky… - Tuberculosis, 2010 - Elsevier... some cases. 22 Blots were purchased from Isogen Biosolutions and run according to the manufacturer's directions. Spoligotyping patterns were converted to the “octal” pattern using the previously described formula. 23 Data ...

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MYCOBACTERIUM MICROTI INFECTION ASSOCIATED WITH SPINDLE CELL PSEUDOTUMOUR AND HYPERCALCAEMIA: A POSSIBLE LINK WITH AN INFECTED ALPACA

C McGoldrick, C Coghlin, A Seagar… - BMJ Case …, 2010 - casereports.bmj.com... At SMRL spoligotyping was performed using a commercial assay available from Isogen Life Science (3600 BT; Maarssen, The Netherlands) and 15-locus Mycobacterial Interspersed Repetitive Unit-Variable Number Tandem Repeat (MIRU-VNTR) typing, performed using the ...

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MOLECULAR EPIDEMIOLOGY OF HUMAN CASES OF TUBERCULOSIS BY MYCOBACTERIUM BOVIS IN MEXICO

F Milián-Suazo, L Pérez-Guerrero… - Preventive veterinary …, 2010 - Elsevier... Coding allows easy comparisons of spoligotypes from different laboratories and from differentparts of ... Spoligotyping was performed following a standard protocol (Kemerbeek et al., 1997). ... 6 spacer sequences from M. bovis BCG were covalently bound (ISOGEN, Maarssen, the ...

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THE MOST PREDOMINANT SPOLIGOPATTERNS OF< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES AMONG IRANIAN, AFGHAN-IMMIGRANT, PAKISTANI AND TURKISH TUBERCULOSIS PATIENTS: …

MA Merza, P Farnia, AM Salih, MR Masjedi… - …, 2010 - content.karger.com

... [19] , with a commercially available kit, according to the manufacturer's instructions (Isogen Biosci-ence, The ... Analysis of Spoligotyping Data A total of 133 distinct spoligopatterns were identi- fied ...The distribution of spoligotypes (T, CAS, EAI, Haarlem, X, Beijing, Or- phan and ...

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[HTML] CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS BEIJING ISOLATES FROM THE MEDITERRANEAN AREA

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M Alonso, NA Rodriguez, C Garzelli… - BMC …, 2010 - biomedcentral.com... deletion of the spacers 1-34 and the characteristic hybridization pattern of spacers 35-43, and the remaining seven corresponded to variant "Beijing-like" spoligotypes. ... Spoligotyping was performed following the manufacturer's instructions (Isogen, Netherlands). ...

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SPOLIGOTYPE-BASED COMPARATIVE POPULATION STRUCTURE ANALYSIS OF MULTIDRUG-RESISTANT AND ISONIAZID-MONORESISTANT MYCOBACTERIUM TUBERCULOSIS COMPLEX CLINICAL ISOLATES …

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T Jagielski, E Augustynowicz-Kopeć… - Journal of clinical …, 2010 - Am Soc Microbiol... CTAB) method (47) were stored at −20°C before being subjected to spoligotyping using a ...hybridized to a set of 43 oligonucleotides covalently bound to a membrane (Isogen Life Science BV ... Spoligotypes in binary format were converted to an octal code for comparison with the ...

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[HTML] FIRST INSIGHTS INTO THE GENETIC DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM HIV-INFECTED MEXICAN PATIENTS AND MUTATIONS CAUSING MULTIDRUG RESISTANCE

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R Lopez-Alvarez, C Badillo-Lopez… - BMC …, 2010 - biomedcentral.com... To determine lineage, the 57 strains (48 MTb and 9 M. bovis) from the MTC were spoligotyped and binary outcomes were compared with the shared type (ST) number and lineages and sublineages reported by ... Spoligotyping. ... Spoligotypes were reported using an octal code [74]. ...

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MOLECULAR EVIDENCE OF FALSE-POSITIVE CULTURES FOR MYCOBACTERIUM TUBERCULOSIS IN A TAIWANESE HOSPITAL WITH A HIGH INCIDENCE OF TB

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CC Lai, CK Tan, SH Lin, CH Liao… - Chest, 2010 - chestjournal.chestpubs.org... Spoligotyping was performed according to the internationally standardized protocol to identify and differentiate ... 10 A commercially available kit (Isogen Bioscience BV; Maarssen, The Netherlands) was ... database was used to assign genotype families to the spoligotypes obtained ...

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MOLECULAR DETECTION OF MIXED INFECTIONS OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN SPUTUM SAMPLES FROM PATIENTS IN KARONGA DISTRICT, MALAWI

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K Mallard, R McNerney, AC Crampin… - Journal of clinical …, 2010 - Am Soc Microbiol... on these isolates with the aid of a commercially available kit (Isogen Biosciences BV ... These isolates were also spoligotyped and had previously been characterized by IS6110-RFLP ... Spoligotyping versus Beijing and LAM PCR results for 377 clinical M. tuberculosis isolates used ...

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[HTML] CLUSTERING OF MYCOBACTERIUM TUBERCULOSIS CASES IN ACAPULCO: SPOLIGOTYPING AND RISK FACTORS

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E Nava-Aguilera, E Harris, S Mitchell… - Clinical and …, 2010 - hindawi.com... DNA with 43 oligonucleotides covalently linked to a nylon membrane (Isogen Bioscience BV ...SpolTools permitted the analysis of M. tuberculosis spoligotyping data to identify emerging ... and visualization of the probable evolutionary relationships between the spoligotypes in our ...

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TRACING THE ORIGINS OF< I> MYCOBACTERIUM BOVIS</I> TUBERCULOSIS IN HUMANS IN THE USA TO CATTLE IN MEXICO USING SPOLIGOTYPING

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TC Rodwell, AJ Kapasi, M Moore… - International Journal of …, 2010 - Elsevier... For this study, we also examined all available spoligotypes in the US national surveillance database of M. bovis isolates sent to the National Veterinary Services Laboratories ... Spoligotyping was performed by all laboratories using the Spoligo Kit (Isogen Bioscience BV ...

MYCOBACTERIUM TUBERCULOSIS BEIJING GENOTYPE IS AN INDEPENDENT RISK FACTOR FOR TUBERCULOSIS TREATMENT FAILURE IN INDONESIA

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I Parwati, B Alisjahbana, L Apriani… - Journal of Infectious …, 2010 - jid.oxfordjournals.org

... of the Beijing genotype per se or to confounding by drug resistance, which has been linked to

the Beijing genotype in different settings [1]. We previously spoligotyped a large ... Spoligotyping

was performed using a commercial kit (Isogen Bioscience) according to the ...

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EFFECT OF SERIAL SUBCULTURING ON THE GENETIC COMPOSITION AND CYTOTOXIC ACTIVITY OF MYCOBACTERIUM TUBERCULOSIS

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CA Molina-Torres, J Castro-Garza… - Journal of medical …, 2010 - Soc General Microbiol... (1997) was used. PCR amplification of the direct repeat locus was performed on heat-treated cell suspensions. Biotin-labelled PCR products were detected by hybridization onto a spoligotyping membrane (Isogen Bioscience). Microarray analysis. ...

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[HTML] DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS GENOTYPES CIRCULATING IN NDOLA, ZAMBIA

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C Mulenga, I Shamputa, D Mwakazanga… - BMC infectious …, 2010 - biomedcentral.com

... Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen, The Netherlands) according ... Spoligotyping and MIRU-VNTR patterns were compared to the

international Spol DB4.0 ... Spoligotypes that were not present in the Spol DB4.0 are referred to ...

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PHENOTYPIC AND GENOTYPIC ANALYSIS OF MULTIDRUG-RESISTANT TUBERCULOSIS IN ETHIOPIA

M Agonafir, E Lemma… - … of Tuberculosis and …, 2010 - ingentaconnect.com

... to the extraction protocol described by van Soolingen et al.14 Spoligotyping was performed ... the

commercially available kit from Isogen Bioscience BV, Maarssen, The Netherlands, according

to ... Spoligotypes described only once (non-clustered) in this study and in the SpolDB4 ...

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[PDF] SENSIBILIDAD Y ESPECIFICIDAD DE PCR ANIDADA Y SPOLIGOTYPING COMO PRUEBAS RÁPIDAS DE DIAGNÓSTICO DE TUBERCULOSIS BOVINA EN TEJIDO FRESCO

[PDF] from scielo.org.mx

FM Suazoa, B Harrisb, CA Díazc… - Revista Mexicana de …, 2010 - scielo.org.mx ... Spoligotyping was performed essentially as described by Kamerbeek(17). Briefly, amplification of DNA was run in ... for 30 sec. Hybridization was performed as recommended by the manufacturer (Isogen). Hybridized DNA was detected ...

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CLINICAL AND GENOTYPIC CHARACTERISTICS OF EXTENSIVELY DRUG-RESISTANT AND MULTIDRUG-RESISTANT TUBERCULOSIS

CC Lai, CK Tan, SH Lin, CH Liao… - European journal of …, 2010 - Springer

... by serial dilution on agar plates as described previously [1]. Spoligotyping, according to ... the M. tuberculosis complex isolates [3]. A commercially available kit (Isogen Bioscience BV ... 1 Dendrograms of spoligotypes generated by clustered Myco- bacterium tuberculosis strains after ...

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IDENTIFICATION OF WILD-TYPE MYCOBACTERIUM TUBERCULOSIS ISOLATES AND POINT MUTATIONS ASSOCIATED WITH ISONIAZID RESISTANCE

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OY Limanskaya, TN Mukhina, VN Stepanshina… - Molecular Biology, 2010 - Springer

... carrying AGC AGA; HB125, carrying AGC ACC, and H37Rv with the wild type codon

315 (negative control for amplification). The reaction mixture (20–50 μl) was prepared

from a dry amplification kit (Isogen, Russia). Its com ...

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A SNAPSHOT OF THE BIODIVERSITY AND CLUSTERING OF MYCOBACTERIUM TUBERCULOSIS IN OMAN USINGSPOLIGOTYPING

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A Al-Maniri, JPN Singh, O Al-Rawas… - … of Tuberculosis and …, 2010 - ingentaconnect.com

... Figure Spoligotyped distribution of ... Molecular typing of Mycobacterium tuberculosis isolates

from a rural area of Kanpur by spoligotyping and mycobacterial interspersed re- petitive units

(MIRUs) typing. ... Predominant tubercu- losis spoligotypes, Delhi, India. ...

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MYCOBACTERIUM TUBERCULOSIS COMPLEX CRISPR GENOTYPING: IMPROVING EFFICIENCY, THROUGHPUT AND DISCRIMINATIVE POWER OF 'SPOLIGOTYPING'WITH NEW SPACERS AND A …

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J Zhang, E Abadia, G Refregier… - Journal of medical …, 2010 - Soc General Microbiol

... Thus, as spoligotypes are considered both as phylogenetic and epidemiological markers (Asiimwe

et al., 2008; Sola et al., 2001; Tanaka & Francis, 2006 ... Both sets had been previously genotyped

by spoligotyping using commercial membranes (Isogen Biosciences). ...

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[HTML] MOLECULAR DIVERSITY OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM PATIENTS WITH TUBERCULOSIS IN HONDURAS

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S Rosales, L Pineda-García… - BMC …, 2010 - biomedcentral.com

... All isolates were spoligotyped in order to identify the predominant genotypes within this ... SG

performed and analyzed RFLP; carried out bioinformatics analysis of spoligotyping and RFLP

results ... NR performed database analysis of the spoligotypes and helped draft the manuscript ...

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[HTML] PRODUCTION OF TNF-Α, IL-12 (P40) AND IL-17 CAN DISCRIMINATE BETWEEN ACTIVE TB DISEASE AND LATENT INFECTION IN A WEST AFRICAN COHORT

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JS Sutherland, BC de Jong, DJ Jeffries, IM Adetifa… - PloS one, 2010 - dx.plos.org... 10ng of DNA was used for spoligotype analysis with commercially available membranes (Isogen Biosciences, The Netherlands). ... Contributed reagents/materials/analysis tools: BCdJ. Wrote the paper: JSS MOCO. Performed spoligotyping: BCdJ. Statistical analysis: DJJ. ...

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[BOOK] RAPID TESTS FOR MULTIDRUG RESISTANT TUBERCULOSIS IN LOW INCOME SETTINGS

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F Bwanga - 2010 - mbl.mak.ac.ug

... In paper V, we applied spoligotyping to study the clustering rate and predominant genotypic strains of 99 MDR-TB strains isolated from patients in Kampala. Eighty three percent of the strains occurred in clusters, and the T2 lineage was the largest single cluster. Conclusion. ...

2009

 

Spoligotyping for molecular epidemiology of the Mycobacterium tuberculosis complex

JR Driscoll - Methods Mol. Biol, 2009 - Springer

... 1. Spoligotyping membrane (Ocimum Biosolutions Inc., for- merly Isogen Biosciences BV ... potential

of additional spacer sequences for “expanded” or “extended” spoligotyping (25, 26). ... spacer

sequences would improve the differentiability of the commonly observed spoligotypes. ...

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Polymorphic Exact Tandem Repeat A (PETRA): a Newly Defined Lineage of Mycobacterium tuberculosis in Israel Originating Predominantly in Sub-Saharan Africa

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PJ Freidlin, D Goldblatt… - Journal of clinical …, 2009 - Am Soc Microbiol

... The fast DNA extraction method (amount used, 2 μl) was also successfully employed for

spoligotyping (Ocimum Biosolution Company, India). ... In addition, further CAS MIRU-VNTR types

and associated spoligotypes were identified in the literature (3, 6, 7, 58, 61, 62). ...

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MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS IN THE UNITED ARAB EMIRATES (UAE)

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A Saaidi… - 2009 - qmro.qmul.ac.uk

... HYPERLINK \l "_Toc237239861" **3.2.11 Identification of the UAE isolates by spoligotyping *

PAGEREF _Toc237239861 \h **133**. * HYPERLINK \l "_Toc237239862" **3.2.12 Comparison

between MIRU-typed and spoligotyped families in association with katG463/gyrA95 ...

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BEIJING GENOTYPE OF MYCOBACTERIUM TUBERCULOSIS IS SIGNIFICANTLY ASSOCIATED WITH HIGH-LEVEL FLUOROQUINOLONE RESISTANCE IN VIETNAM

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DD An, NTH Duyen, NTN Lan, DTM Ha… - Antimicrobial agents …, 2009 - Am Soc Microbiol

... with primers Dra (biotin labeled) and Drb, hybridized to the spoligotype membrane (Isogen

Bioscience BV ... were assigned to the Euro-American clade on the basis of spoligotyping patterns ...

LSP types and spoligotypes of 109 consecutive FQ-resistant isolates and 109 consecutive ...

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[PDF] NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS

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TB Nosocomial - Indian J Tuberc, 2009 - lrsitbrd.nic.in

Page 1. Indian Journal of Tuberculosis [Indian J Tuberc 2009; 56:59-61] Editorial

NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS

Indian Journal of Tuberculosis Vol. 56 New Delhi, April 2009 No. 2 ...

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[HTML] SUPERVISED LEARNING FOR THE AUTOMATED TRANSCRIPTION OF SPACER CLASSIFICATION FROM SPOLIGOTYPE FILMS

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D Jeffries, N Abernethy… - BMC bioinformatics, 2009 - biomedcentral.com

... a standardized method [4] using commercially prepared membranes from Isogen Biosciences

(Maarsen ... Crawford JT: Transfer of a Mycobacterium tuberculosis genotyping method,

Spoligotyping, from a ... AR, Tanaka MM: spolTools: online utilities for analyzing spoligotypes of ...

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POLYMORPHIC EXACT TANDEM REPEAT A (PETRA): A NEWLY DEFINED LINEAGE OF MYCOBACTERIUM TUBERCULOSIS IN ISRAEL ORIGINATING PREDOMINANTLY IN SUB-SAHARAN AFRICA

[HTML] from nih.gov

PJ Freidlin, D Goldblatt… - Journal of clinical …, 2009 - Am Soc Microbiol... In addition, further CAS MIRU-VNTR types and associated spoligotypes were identified in ...Spoligotyping.Spacer oligonucleotide typing (spoligotyping) for 43 spacers of the direct repeat region (9 ... performed using a kit according to the manufacturer's instructions (Isogen/Omicium ...

A RAPID AND SIMPLE METHOD FOR IDENTIFYING MYCOBACTERIUM TUBERCULOSIS W-BEIJING STRAINS BASED ON DETECTION OF A UNIQUE MUTATION IN RV0927C BY PCR-SSCP

X Jiang, C Lu, F Gao, F Wang, W Zhang… - Microbes and …, 2009 - Elsevier

... Spoligotyping was performed as previously described [16 ... in 2 × SSPE (1 × SSPE is 0.18 M NaCl, 10 mM NaH 2 PO 4 , and 1 mM EDTA) −0.5% sodium dodecyl sulfate (SDS) at 60 °C and then incubated in 1:4000 diluted streptavidin peroxidase conjugate (Isogen Bioscience) for ...

Using a multiplex polymerase chain reaction for the identification of Beijing strains of Mycobacterium tuberculosis

JR Sun, SY Lee, HY Dou… - European Journal of Clinical …, 2009 - Springer... Page 2. in the Isogen Bioscience spoligotyping kit (Isogen Life Science, the Netherlands). ... Among the 323 clinical MTB isolates, a total of 78 different spoligotypes were found. One hundred and sixty-nine isolates were determined to belong to the Beijing family. ...

[HTML] Mycobacterium bovis en Argentina: aislamientos de gatos tipificados por spoligotyping

[HTML] from scielo.org.ar

MJ Zumárraga, M Martínez Vivot… - Revista argentina de …, 2009 - SciELO Argentina

... (8) , and using the spoligotyping kit (Isogen Biosolutions BV ... Each of the different spoligotypes were allocated a number. ... H37Rv (ATCC 27294) and M. bovis Bacillus Calmette-Guerin (BCG)(ATCC 27289) were included as reference strains in each spoligotyping experiment. ...

Spoligotyping for molecular epidemiology of the Mycobacterium tuberculosis complex

JR Driscoll - Methods Mol. Biol, 2009 - Springer... 1. Spoligotyping membrane (Ocimum Biosolutions Inc., for- merly Isogen Biosciences BV ... potential of additional spacer sequences for “expanded” or “extended” spoligotyping (25, 26). ... spacer sequences would improve the differentiability of the commonly observed spoligotypes. ...

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Use of spoligotyping and large sequence polymorphisms to study the population structure of the Mycobacterium tuberculosis complex in a cohort study of consecutive …

[HTML] from nih.gov

BC De Jong, M Antonio, T Awine… - Journal of clinical …, 2009 - Am Soc Microbiol... After spectrophotometry, we used 10 ng of DNA for spoligotype analysis (16) with commercially available membranes (Isogen Biosciences, Maarssen, The Netherlands ... If spoligotype analysis did not result in a clear pattern of spacers, the isolate underwent repeat spoligotyping. ...

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Isoniazid and rifampicin resistance-associated mutations in Mycobacterium tuberculosis isolates from Yangon, Myanmar: implications for rapid molecular testing

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H Valvatne, H Syre, M Kross… - Journal of …, 2009 - Br Soc Antimicrob Chemo

... identification test (GenProbe Incorporated, San Diego, CA, USA) and by spoligotyping (Isogen Bioscience BV ... A total of 43 different spoligotypes were observed among the 96 isoniazid-resistant … of the 63 isolates that could not be differentiated by spoligotyping produced diverse ...

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… of the Mycobacterium tuberculosis complex in Albania obtained by multilocus variable-number tandem-repeat analysis and spoligotyping reveals the presence of …

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S Tafaj, J Zhang, Y Hauck, C Pourcel… - Journal of clinical …, 2009 - Am Soc Microbiol... ethambutol (2.0 μg/ml) (5). Identification at the species level was performed by phenotypic methods and verified by a genotypic method on isolates with rare spoligotypes and/or ... Spoligotyping was performed using a commercial membrane (Isogen, The Netherlands ...

Prospective study applying spoligotyping directly to DNA from sputum samples of patients suspected of having tuberculosis

PICPI Cafrune, LGPLG Possuelo… - Canadian Journal …, 2009 - NRC Research Press ... was performed using a commercial kit according to the manufacturer's instructions (Isogen Biosciences BV ... Table 1. Accuracy of spoligotyping results according to culture grading. ... A total of 44 different spoligotypes were identified; 112 isolates (83%) were included in 21 shared ...

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Molecular typing of Mycobacterium bovis strains isolated in Italy from 2000 to 2006 and evaluation of variable-number tandem repeats for geographically optimized …

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MB Boniotti, M Goria, D Loda… - Journal of clinical …, 2009 - Am Soc Microbiol... repeat locus were detected by hybridization onto a spoligotyping membrane (Isogen Bioscience BV ... into 64 different patterns, while the seven M. caprae spoligotypes were subdivided ... Thediscrimination provided by combination of spoligotyping and ETR methods allowed for a ...

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Utility and evaluation of new variable-number tandem-repeat systems for genotyping mycobacterial tuberculosis isolates

HY Dou, JJ Lu, CW Lin, JR Chang, JR Sun… - Journal of microbiological …, 2009 - Elsevier... Spoligotyping was carried out using a spoligotyping kit according to the manufacturer's instructions (Isogen Bioscience BV). Spoligotypes common to more than one strain were designated as shared types (ST) and assigned a shared international type number (SIT) according to ...

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[PDF] Comparison of phenotypic and genotypic methods for pyrazinamide susceptibility testing

[PDF] from medind.nic.in

S Shenai, C Rodrigues, M Sadani, N Sukhadia… - Indian J Tuberc, 2009 - medind.nic.in... All the PZA resistant isolates were further spoligotyped to identify M. bovis, if present. ... Finger printing - Spoligotyping All the 63 culture isolates were subjected to spoligotyping using a commercially available Isogen kit, Netherlands as described by Kamerbeek et al.11 Briefly ...

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Molecular analysis of< i> Mycobacterium tuberculosis</i> from tuberculous meningitis patients in Thailand

J Yorsangsukkamol, A Chaiprasert, T Prammananan… - Tuberculosis, 2009 - Elsevier... 2.4. Spoligotyping. Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarsen, The Netherlands). ... 25 All obtained spoligotypes were compared to SpolDB4 for analysis. 2.5. Data analysis. ...

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Prevalence of ST26 among untreated smear-positive tuberculosis patients from Karachi indicating ongoing transmission

S Shakoor, M Tanveer, Y Rafiq… - Scandinavian …, 2009 - informahealthcare.com... Five different resistance phenotypes were identified among the isolates spoligotyped: isolated low-level isoniazid resistance (resistant to 0.2 µg/ml) in 1 isolate; high-level isoniazid resistance(resistant to 1 ... Spoligotypes Spoligotyping grouped 66 isolates into 9 different clusters. ...

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[PDF] Reinout van Crevel1, Ida Parwati2, Edhyana Sahiratmadja2, Sangkot Marzuki3, Tom HM Ottenhoff4, Mihai G. Netea1, Andre van der Ven1, Ronald H. Nelwan5, …

[PDF] from kun.nl

BA van der Meer… - Factors Underlying the Success … - repository.ubn.kun.nl... min. Spoligotyping was performed using a commercial kit (Isogen Bioscience,Maarssen, The Netherlands). The ... min. Spoligotyping was performed using acommercial kit (Isogen Bioscience, Maarssen, The Netherlands). The ...

Emergence of new forms of totally drug-resistant tuberculosis bacilli

[HTML] from chestpubs.org

AA Velayati, MR Masjedi, P Farnia… - Chest, 2009 - chestjournal.chestpubs.org... by polymerase chain reaction (PCR) using primers derived from the direct repeat sequence (Isogen Bioscience; Maarssen, the ... and CAS (n = 4; 17.5%) [Table 2]. The VNTR profiles of spoligotypes strains were ... Spoligotyping Patterns and VNTR Profiles of XDR and TDR Patients. ...

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[PDF] Mycobacterium tuberculosis Beijing genotype in Indonesia is associated with drug resistance gene mutations in katG, rpoB and embB

[PDF] from kun.nl

I Parwati, R van Crevel, J de Beer… - Factors Underlying the … - repository.ubn.kun.nl

... of rpoB-hotspot region Three hundred and twenty five spoligotyped isolates were ... Mycobacteriumtuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4 ... mutations in katG, rpoB, rpsL and rrs genes with spoligotypes of multidrug ...

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Infection with Mycobacterium tuberculosis Beijing genotype strains is associated with polymorphisms in SLC11A1/NRAMP1 in Indonesian patients with tuberculosis

[HTML] from oxfordjournals.org

R Van Crevel, I Parwati… - Journal of Infectious …, 2009 - jid.oxfordjournals.org... Mycobacterial DNA was extracted by bringing 2 loops of bacterial mass from a M. tuberculosis culture in saline and subsequently heating it at 95°C for 5 min. Spoligotyping was performed using a commercial kit (Isogen Bioscience). ...

Unexpectedly high proportion of ancestral Manu genotype Mycobacterium tuberculosis strains cultured from tuberculosis patients in Egypt

[HTML] from nih.gov

ZH Helal, MSED Ashour, SA Eissa… - Journal of clinical …, 2009 - Am Soc Microbiol... was amplified with primers DRa and DRb, and the amplified biotinylated products were hybridized to a set of 43 oligonucleotides covalently bound to a membrane (Isogen Life Science BV ...Comparison of spoligotyping results with database types.Spoligotypes in binary ...

Differences in Cell Wall Thickness between Resistant and Nonresistant Strains of< i> Mycobacterium tuberculosis</i>: Using Transmission Electron Microscopy

[PDF] from mrc.ac.ir

AA Velayati, P Farnia, TA Ibrahim… - …, 2009 - content.karger.com... For spoligotyping, the direct repeat region was am- plified by PCR using primers derived fromthe direct repeat se- quence (Isogen Bioscience). ... Spoligopatterns and VNTR Profiles When the spoligotype patterns were compared with earlier published spoligotypes [5] , our ...

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Mycobacterium tuberculosis genotypic diversity in pyrazinamide-resistant isolates of Iran

[PDF] from mrc.ac.ir

F Doustdar, AD Khosravi… - Microbial Drug …, 2009 - online.liebertpub.com... Spoligotyping was performed with a commercial kit (Isogen Bioscience BV, Maarsen, The ... Only1 (5.0%) of the 20 PZA-monoresistant isolates had spoligotyping patterns characteristic ... Therefore,the CAS superfamily spoligotypes showed no important correlation with MDR-TB as ...

Molecular characterisation of Mycobacterium bovis isolates from cattle carcases at a city slaughterhouse in Uganda

BB Asiimwe, J Asiimwe, G Kallenius… - Veterinary …, 2009 - veterinaryrecord.bmj.com... The isolates were spoligotyped using the DNA extracted at the identification stage. ... Thespoligotypes were analysed by BioNumerics software, version 5.0 (Applied Maths) as character ...were compared with those in SpolDB4.0, the international spoligotyping database (Brudey ...

High resolution typing system for pathogenic Mycobacterium tuberculosum

PS Keim, RS Spurgiesz… - US Patent 7,592,135, 2009 - Google Patents... Spoligotyping is currently the most widely used PCR- based method of M. tuberculosum genotyping due to the 65 relative technical ease of ... In a database of 3,319 spoligotypes from isolates collected in geographically distinct areas, seven spoligotype patterns were found for ...

Frequency of Mycobacterium bovis as an etiologic agent in extrapulmonary tuberculosis in HIV-positive and-negative Mexican patients

R Cicero, H Olivera, A Hernández-Solis… - European journal of …, 2009 - Springer

... a commercially available kit in accordance with the manufacturer's recommendations (Isogen Bioscience BV ... and consistent identification of M. bovis was obtained by spoligotyping, showing a ... cattle, where even isolates from the same herd show different spoligotypes [26] and ...

[HTML] In-depth molecular characterization of Mycobacterium tuberculosis from New Delhi–predominance of drug resistant isolates of the 'modern'(TbD1−) type

[HTML] from plos.org

R Stavrum, VP Myneedu, VK Arora, N Ahmed… - PloS one, 2009 - dx.plos.org... C membranes with the 43 synthetic oligonucleotides covalently bound to the membranes (Isogen Bioscience BV ... (2004) Predominant tuberculosis spoligotypes, Delhi, India. ... repetitive-unit-variable-number tandem-repeat typing system combined with spoligotyping for population ...

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Predominance of Mycobacterium tuberculosis EAI and Beijing lineages in Yangon, Myanmar

[HTML] from nih.gov

S Phyu, R Stavrum, T Lwin… - Journal of clinical …, 2009 - Am Soc Microbiol... (35). Spoligotyping was performed with commercially available activated Biodyne C membranesthat had 43 synthetic oligonucleotides covalently bound to the membranes (Isogen BioscienceBV ... Prevalence of spoligotypes in Yangon.The spoligotyping results obtained ...

[HTML] Tokyo-172 BCG vaccination complications, Taiwan

[HTML] from nih.gov

R Jou, WL Huang… - Emerging Infectious Diseases, 2009 - ncbi.nlm.nih.gov... BCG, DNA samples were initially screened using a GenoType kit (Hain Lifescience GmbH, Nehren,Germany), multiplex PCR (7), and pncA sequencing (8). In addition, spoligotyping was performedwith a commercial kit (Isogen Bioscience BV, Maarssen, the Netherlands). ...

Immunologic analysis of HIV-uninfected taiwanese children with BCG-induced disease

WI Lee, FC Liang, JL Huang, TH Jaing… - Journal of clinical …, 2009 - Springer... also used to identify and differentiate M. tuberculosis complex isolates in this study [38], using a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands). The resolved spoligotypes were M. tuberculosis complex strain specific. The spoligotyping profile of ...

A rapid and simple method for identifying Mycobacterium tuberculosis W-Beijing strains based on detection of a unique mutation in Rv0927c by PCR-SSCP

X Jiang, C Lu, F Gao, F Wang, W Zhang… - Microbes and …, 2009 - Elsevier... Spoligotyping was performed as previously described [16 ... in 2 × SSPE (1 × SSPE is 0.18 M NaCl,10 mM NaH 2 PO 4 , and 1 mM EDTA) −0.5% sodium dodecyl sulfate (SDS) at 60 °C and thenincubated in 1:4000 diluted streptavidin peroxidase conjugate (Isogen Bioscience) for ...

[PDF] NOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSIS

[PDF] from lrsitbrd.nic.in

TB Nosocomial - Indian J Tuberc, 2009 - lrsitbrd.nic.inPage 1. Indian Journal of Tuberculosis [Indian J Tuberc 2009; 56:59-61] EditorialNOSOCOMIAL TUBERCULOSIS IN THE ERA OF DRUG RESISTANT TUBERCULOSISIndian Journal of Tuberculosis Vol. 56 New Delhi, April 2009 No. 2 ...

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Beijing genotype of Mycobacterium tuberculosis is significantly associated with high-level fluoroquinolone resistance in Vietnam

[HTML] from nih.gov

DD An, NTH Duyen, NTN Lan, DTM Ha… - Antimicrobial agents …, 2009 - Am Soc Microbiol... with primers Dra (biotin labeled) and Drb, hybridized to the spoligotype membrane (IsogenBioscience BV ... were assigned to the Euro-American clade on the basis of spoligotyping patterns ...LSP types and spoligotypes of 109 consecutive FQ-resistant isolates and 109 consecutive ...

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Polymorphic Exact Tandem Repeat A (PETRA): a Newly Defined Lineage of Mycobacterium tuberculosis in Israel Originating Predominantly in Sub-Saharan Africa

[HTML] from nih.gov

PJ Freidlin, D Goldblatt… - Journal of clinical …, 2009 - Am Soc Microbiol... In addition, further CAS MIRU-VNTR types and associated spoligotypes were identified in ...Spoligotyping.Spacer oligonucleotide typing (spoligotyping) for 43 spacers of the direct repeatregion (9 ... performed using a kit according to the manufacturer's instructions (Isogen/Omicium ...

[HTML] Supervised learning for the automated transcription of spacer classification from spoligotype films

[HTML] from biomedcentral.com

D Jeffries, N Abernethy… - BMC bioinformatics, 2009 - biomedcentral.com... a standardized method [4] using commercially prepared membranes from Isogen Biosciences (Maarsen ... Crawford JT: Transfer of a Mycobacterium tuberculosis genotyping method, Spoligotyping, from a ... AR, Tanaka MM: spolTools: online utilities for analyzing spoligotypes of ...

Potential challenges to the Stop TB Plan for humans in China; cattle maintain M. bovis and M. tuberculosis

[PDF] from yimg.com

Y Chen, Y Chao, Q Deng, T Liu, J Xiang, J Chen… - Tuberculosis, 2009 - Elsevier... Spoligotyping was performed to define the genotype of Beijing family strains with a commercial kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the manufacturer's instruction. The spoligotype that only contained ...

[PDF] Tuberculosis Control in Oman

[PDF] from ki.se A Al-Maniri - 2009 - diss.kib.ki.se... Spoligotyping of 312 isolates of Mycobacterium tuberculosis from TB patientsdiagnosed during 2005-2007 was carried out in study IV. ... 25 6.5 Study IV: Biodiversity and clustering of M.tuberculosis isolates using spoligotyping ..... ...

[HTML] Tuberculosis disparity between US-born blacks and whites, Houston, Texas, USA

[HTML] from nih.gov

JA Serpa, LD Teeter, JM Musser… - Emerging infectious …, 2009 - ncbi.nlm.nih.gov... isolates underwent susceptibility and genotype testing (insertion sequence 6110 restriction fragment length polymorphism, spoligotyping, and genetic ... the spacer oligonucleotide type (spoligotype) was determined by using a commercially available kit (Isogen Bioscience BV ...

Molecular characterization of Mycobacterium Bovis isolates from selected slaughter houses in Kampala

[PDF] from mak.ac.ug

J Asiimwe - 2009 - dspace.mak.ac.ug... Spoligotyping revealed that three of the M. bovis isolates had spoligopatterns that had previously been reported in Uganda while the seven ... Although a few clusters were identified on analysis of the spoligotypes and RFLP patterns, no geographical sub-structuring was observed ...

Predictors of poor treatment outcome in multi-and extensively drug-resistant pulmonary TB

[HTML] from ersj.org.uk

K Kliiman… - European Respiratory Journal, 2009 - ersj.org.uk... The strains were spoligotyped as described by Kamerbeek et al. 17 using commercially available membranes (Isogen, Maarssen, The Netherlands) 17. ... Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for ...

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[HTML] Tuberculosis Disparity between US-born Blacks and Whites, Houston, Texas, USA

[HTML] from cdc.gov

F Figures, I Capitalization, AS Guides… - Tuberculosis, 2009 - wwwnc.cdc.gov... isolates underwent susceptibility and genotype testing (insertion sequence 6110 restriction fragment length polymorphism, spoligotyping, and genetic ... the spacer oligonucleotide type(spoligotype) was determined by using a commercially available kit (Isogen Bioscience BV ...

 

2008

 

Spoligotypes of< i> Mycobacterium tuberculosis</i> isolates of a high tuberculosis burden aboriginal township in Taiwan

PC Chuang, H Liu, C Sola, YMA Chen… - Infection, Genetics and …, 2008 - Elsevier... A commercially available spoligotyping kit (Isogen Bioscience BV, Maarssen, The Netherlands) was used as ... All 138 isolates were successfully genotyped by spoligotyping, but only 122 were of ... A total of 27 spoligotypes were identified; 124 isolates (89.9%, 124/138) were ...

Genetic diversity of isoniazid-resistant Mycobacterium tuberculosis isolates collected in Poland and assessed by spoligotyping

[HTML] from nih.gov

E Augustynowicz-Kopeć, T Jagielski… - Journal of clinical …, 2008 - Am Soc Microbiol... Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands), according to the manufacturer's instructions and as ... All spoligotypes obtained were compared to those in the world spoligotyping database (SpolDB4) at ...

[HTML] Mycobacterium tuberculosis spoligotypes and drug susceptibility pattern of isolates from tuberculosis patients in peri-urban Kampala, Uganda

[HTML] from biomedcentral.com

B Asiimwe, S Ghebremichael… - BMC infectious …, 2008 - biomedcentral.com... The obtained signatures were compared in binary format with the international spoligotyping\ database of the Pasteur Institute of ... Spoligotypes. To determine the strain lineages present in the sample, the 344 isolates were spoligotyped and binary outcomes compared with those ...

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Molecular typing of Mycobacterium tuberculosis isolates from a rural area of Kanpur by spoligotypingand mycobacterial interspersed repetitive units (MIRUs) typing

P Sharma, DS Chauhan, P Upadhyay… - Infection, Genetics and …, 2008 - Elsevier

... 2.3. Spoligotyping. Spoligotyping to detect 43 spacers was done with the help of commercially available kit (Isogen Biosciences, BV, Maarsen, The Netherlands). ... 3.1. Spoligotyping. A total of 46 distinct spoligotypes were obtained from this set of 97 M. tuberculosis isolates. ...

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Role of spoligotyping and IS6110-RFLP in assessing genetic diversity of< i> Mycobacterium tuberculosis</i> in India

JP Mathuria, P Sharma, P Prakash… - Infection, Genetics and …, 2008 - Elsevier... spacers by using the standard method (Kamerbeek et al., 1997) with the help of commercially available kit (Isogen Biosciences, BV ... Spoligotyping. ... Presence of 19 orphan isolates indicates the need of conducting multicentric studies to know all the prevalent spoligotypes in Indian ...

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[PDF] Investigation of Cross-Contamination in a Mycobacterium tuberculosis Laboratory Using Epidemiological data and Spoligotyping

[PDF] from pjms.com.pk

F Al-Otaibi… - pjms.com.pk... Spoligotyping was performed with a commer- cially available kit (Isogen Bioscience).6,7 RESULTS ...Culturing of phosphate buffer in MGIT tube was positive for MTB after seven-day inoculation. Ten different spoligotypes were identified among the 47 MTB isolates. ...

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Persistence and molecular evolution of Mycobacterium bovis population from cattle and wildlife in Doñana National Park revealed by genotype variation

[PDF] from cnrs.fr

B Romero, A Aranaz, Á Sandoval, J Álvarez… - Veterinary …, 2008 - Elsevier... detected by hybridization of the biotin-labelled PCR product onto spoligotyping membrane (Isogen Bioscience BV ... The discriminatory capacity of spoligotyping was h = 0.38 ... The spoligotypes SB0950, SB1230 and SB1232 were shared between domestic and wildlife animals (red ...

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Spoligotyping of Mycobacterium tuberculosis in anthracotic bronchitis

K Najafizadeh, F Ghorbani, P Farnia… - … of Tuberculosis and …, 2008 - ingentaconnect.com... use at −20°C. Spoligotyping Spoligotyping was performed, as previously described by Kamerbeek et al.,10 with a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands). Analysis Information about the ...

Molecular analysis of drug‐resistant Mycobacterium tuberculosis isolates collected in central Poland

E Augustynowicz‐Kopeć, T Jagielski… - Clinical …, 2008 - Wiley Online Library... DNA was prepared by the cetyl-trimethyl-ammonium bromide method [4]. Spoligotyping was performed using a commercially available kit (Isogen Bioscience BV ... was also performed as described previously [2]. Isolates with indistinguishable spoligotypes and IS6110 ...

[HTML] Human tuberculosis caused by Mycobacterium bovis, Taiwan

[HTML] from nih.gov

R Jou, WL Huang… - Emerging infectious diseases, 2008 - ncbi.nlm.nih.gov... Currently, 119 M. bovis spoligotypes are contained in the fourth international spoligotyping database (SpolDB 4) and ... strains, presence or absence of region of difference 1 was analyzed(5). Spoligotyping was performed with a commercial kit (Isogen Bioscience BV ...

A proposal for applying molecular markers as an aid to identifying potential cases of imported tuberculosis in immigrants

M Alonso, S Borrell, MM Lirola, E Bouza… - Tuberculosis, 2008 - Elsevier... Spoligotyping was performed using primers of the spoligotyping kit (Isogen, Netherlands) andfollowing the manufacturer's instructions. The spoligotypes were analyzed in the MIRU-VNTRplus application (www.miru-vntrplus.org) 19 in order to identify the genetic lineages ...

Characterization of extensively drug-resistant Mycobacterium tuberculosis clinical isolates in China

[HTML] from nih.gov

Z Sun, Y Chao, X Zhang, J Zhang… - Journal of clinical …, 2008 - Am Soc Microbiol... repeat (MIRU-VNTR) analysis. Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the manufacturer's protocols. PCR-based MIRU-VNTR analysis ...

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[HTML] Incidence of tuberculosis and the predictive value of ELISPOT and Mantoux tests in Gambian case contacts

[HTML] from plos.org

PC Hill, DJ Jackson-Sillah, A Fox, RH Brookes… - PLoS One, 2008 - dx.plos.org... For molecular subtyping of index case isolates, we extracted mycobacterial DNA using CTAB and chloroform[18]. We performed spoligotyping using membranes from Isogen Biosciences (http:/www.isogen-lifescience.com), as previously described[19]. ...

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[PDF] : Transmission from South American Sea Lion () to Bactrian Camel () and Malayan Tapirs ()

[PDF] from cnrs.fr

I Moser, WM Prodinger, H Hotzel… - Veterinary …, 2008 - peer.ccsd.cnrs.fr... by 30 cycles of 96°C for 15 sec, 67°C for 60 sec, 72°C for 60 sec, and a final extension at 18 72°C 300 sec. 19 Spoligotyping. Spoligotyping (9; Isogen Bioscience, The Netherlands) was performed using 20 DNA extracted from cultures. 21 MIRU typing. ...

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Use of PCR-based Mycobacterium tuberculosis genotyping to prioritize tuberculosis outbreak control activities

[HTML] from nih.gov

M Ashworth, KL Horan, R Freeman… - Journal of clinical …, 2008 - Am Soc Microbiol... Only one isolate per case was included in the analysis. Genotyping.Spoligotyping was performed according to previously published methods by using a spoligotypingkit (Isogen, IJsselstein, The Netherlands) (12). MIRU analysis ...

Mycobacteria causing human cervical lymphadenitis in pastoral communities in the Karamoja region of Uganda

[HTML] from nih.gov

J Oloya, J Opuda-Asibo, R Kazwala… - Epidemiology and …, 2008 - Cambridge Univ Press... MTC were submitted for further differentiation by the spoligotyping kit ac- cording to the manufacturer's instructions (Isogen, Life Science ... One of the spoligotypes (Hu-4 and Hu-5 in Fig. 1) is labelled LAM 3/S in the spoligotyping database SpolDB4, and has been isolated in 118 ...

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Genotypic diversity of extensively drug-resistant tuberculosis (XDR-TB) in South Africa

CK Mlambo, RM Warren, X Poswa… - … of tuberculosis and …, 2008 - ingentaconnect.com... Spoligotyping was performed ac- cording to the internationally standardised protocol11 using commercially ... of the genotyping data, serial isolates from 13 patients were randomly sampled and spoligotyped. ... was used to assign geno- type families to the spoligotypes obtained.12 ...

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[HTML] Molecular epidemiology and evolutionary genetics of Mycobacterium tuberculosis in Taipei

[HTML] from biomedcentral.com

HY Dou, FC Tseng, CW Lin, JR Chang… - BMC infectious …, 2008 - biomedcentral.com... Of 356 isolates spoligotyped, 290 (81.4%) displayed known spoligotypes and 66 were not ...Spoligotyping was carried out according to the manufacturer's instructions (Isogen Bioscience BV ... The resulting spoligotypes were documented under a binary code representing either a ...

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[HTML] Genotyping and drug resistance patterns of M. tuberculosis strains in Pakistan

[HTML] from biomedcentral.com

M Tanveer, Z Hasan, A Siddiqui, A Ali… - BMC Infectious …, 2008 - biomedcentral.com... Spoligotyping of M. tuberculosis isolates. All M. tuberculosis strains were spoligotyped and their data analyzed using UPGMA calculation in the Bionumerics software. ... one (78%) isolates grouped into 59 different "shared types" while 205 (22%) isolates had 'orphan' spoligotypes. ...

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< i> Mycobacterium pinnipedii</i>: Transmission from South American sea lion (< i> Otaria byronia</i>) to Bactrian camel (< i> Camelus bactrianus bactrianus</i>) and …

[PDF] from tcd.ie

I Moser, WM Prodinger, H Hotzel, R Greenwald… - Veterinary …, 2008 - Elsevier... 2.5. Spoligotyping. Spoligotyping (Kamerbeek et al., 1997; Isogen Bioscience, The Netherlands) was performed using DNA extracted from cultures. 2.6. MIRU typing. MIRU typing was performed targeting 12 chromosomal loci as given by Mazars et al. ...

Mycobacterium tuberculosis population structures differ significantly on two Indonesian Islands

[HTML] from nih.gov

I Parwati, R Van Crevel, M Sudiro… - Journal of clinical …, 2008 - Am Soc Microbiol... Therefore, we have spoligotyped M. tuberculosis isolates from a large cohort of patients ...Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for ... Global distribution of Mycobacterium tuberculosis ...

Development of multiplex assay for rapid characterization of Mycobacterium tuberculosis

[HTML] from nih.gov

IL Bergval, R Vijzelaar, ER Dalla Costa… - Journal of clinical …, 2008 - Am Soc Microbiol... Strains 1 to 29 were selected for their specific spoligotypes and were used to determine the correlation between MLPA, the spoligotype, and the sequence of ogt codon 15 (a marker for theM ... Spoligotyping.Mycobacterium tuberculosis strains were spoligotyped by using a ...

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Mycobacterium bovis in wildlife in France

[HTML] from jwildlifedis.org

G Zanella, B Durand, J Hars… - Journal of wildlife …, 2008 - Wildlife Dis Assoc... Biotin-labeled PCR products were detected on a spoligotyping membrane (Isogen Bioscience BV,Maarssen, The Netherlands). Purified sterile water and M. bovis and M. tuberculosis controlsincluded in the spoligomembrane kit were included in each batch of tests. ...

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Distribution of rpoB mutations among multidrug‐resistant Mycobacterium tuberculosis (MDRTB) strains from Thailand and development of a rapid method for mutation …

T Prammananan, W Cheunoy… - Clinical …, 2008 - Wiley Online Library... In contrast, of 112 rifampicin-susceptible isolates that were spoligotyped, only 51 (45.5%) wererevealed to be ... a One additional MDRTB isolate was not available for spoligotyping. ... Association of specific mutations in katG, rpoB, rpsL and rrs genes with spoligotypes of multidrug ...

Molecular diversity of< i> Mycobacterium tuberculosis</i> isolates from patients with pulmonary tuberculosis in Sri Lanka

US Rajapaksa, TC Victor, AJ Perera… - Transactions of the …, 2008 - Elsevier... Spoligotyping was performed using a commercially available kit (Isogen Bioscience BV, Maarssen,The ... Spoligotyping of 98 M. tuberculosis isolates revealed a total of 43 patterns, with six ... The finding of identical spoligotypes in geographically widely separated countries may be ...

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[PDF] Mutations in rpoB gene and genotypes of rifampin resistant Mycobacterium tuberculosis isolates in Iran

[PDF] from nritld.ac.ir

F Doustdar, AD Khosravi, P Farnia, AR Bahrmand… - Tanaffos, 2008 - nritld.ac.ir

... tuberculosis isolates are defined on the basis of shared spoligotypes or closely related IS6110 ...Spoligotyping was performed with a commercially available kit to detect 43 known spacers ... DR locus according to the instructions supplied by the manufacturer (Isogen Bioscience BV ...

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Molecular epidemiology of Mycobacterium bovis: usefulness in international trade

[PDF] from usda.gov

F Milian-Suazo, B Harris, CA Díaz… - Preventive veterinary …, 2008 - Elsevier... Spoligotyping was performed following the protocol described by Kamerbeek et al ... Eighty-four M. bovis strains from Mexico and the US were spoligotyped under the same protocol in two ... When comparing spoligotypes from the two laboratories, 50 (59%) out of the 84 strains were ...

Attempts to revive Mycobacterium tuberculosis from 300‐year‐old human mummies

E Lemma, O Zimhony, CL Greenblatt… - FEMS microbiology …, 2008 - Wiley Online Library... Spoligotyping was performed at day 250 following the instructions of the manufacturer of the ISOGEN Life Science Spoligotyping kit. Animal inoculations. Two sets of six female guinea pigs weighing an average of 250 g were used. ...

Mycobacterium tuberculosis Uganda genotype is the predominant cause of TB in Kampala, Uganda

[PDF] from mak.ac.ugBB Asiimwe, T Koivula, G Kallenius… - … of Tuberculosis and …, 2008 - ingentaconnect.com... Data analysis Spoligotypes were analysed using BioNumerics soft- ware (version 4.6, Applied Maths, Kortrijk, Belgium) as a character type. ... Spoligotyping To determine the predominant strain lineages, the 344 isolates were spoligotyped and binary outcomes compared with ...

Pyrazinamide resistance among South African multidrug-resistant Mycobacterium tuberculosis isolates

[HTML] from nih.gov

M Mphahlele, H Syre, H Valvatne… - Journal of clinical …, 2008 - Am Soc Microbiol

... (i) Spoligotyping.Spoligotyping was performed to further ... The resulting amplification products were then hybridized to a commercially available membrane (Isogen Bioscience BV, Bilthoven, The Netherlands), with covalently linked parallel rows of 43 synthetic oligonucleotides ...

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Genotyping North American animal Mycobacterium bovis isolates using multilocus variable number tandem repeat analysis

[HTML] from sagepub.com

LR Martinez, B Harris, WC Black… - Journal of Veterinary …, 2008 - vdi.sagepub.com... The 6 isolates that comprised MLVA type N were divided into 3 spoligotypes: 1 isolate into SP2 ...A larger database of MLVA typed isolates, in combination with spoligotyping and conventional epidemiological investigations, can provide a ... n. Spacer sequences, Isogen Life Science ...

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[CITATION] First Insight Into Mycobacterium tuberculosis Biodiversity in Sri Lanka: Evidence for Predominance of Beijing Genotype and Ancestral Lineages Among …US Rajapaksa, AJ Perera, TC Victor… - International Journal of …, 2008 - Elsevier

 

[BOOK] Molecular characterization of Mycobacterium tuberculosis complex from Kampala, Uganda

[PDF] from mak.ac.ug

B Asiimwe - 2008 - dspace.mak.ac.ug... and colleagues (69), which is commercially available as a kit (Isogen Bioscience BV ... the important lineages and sub-lineages in SpolDB4.0, the international spoligotyping database iii ... To investigate the association between the predominant spoligotypes, HIV sero- status and anti ...

IS6110 in oriC affects the morphology and growth of Mycobacterium tuberculosis and attenuates virulence in mice

Y Casart, L Turcios, I Florez, R Jaspe, E Guerrero… - Tuberculosis, 2008 - Elsevier... DR locus was carried out as described earlier, 15 with membranes supplied by Isogen Bioscience BV ... to mention that all the clones isolated from 1C8 or 1D3 displayed identical spoligotyping and MIRU ... A1 insertion 12 (Figure 1B), (ii) The IS6110-RFLPs and spoligotypes did not ...

 

Intérêt du typage moléculaire pour l'épidémiologie de la tuberculose en Limousin (1998–2006)

[HTML] from em-consulte.com

H Bezanahary, MC Baclet, C Sola… - Médecine et Maladies …, 2008 - Elsevier... Keywords: MIRU15 typing; Mycobacterium tuberculosis; Spoligotyping. ... L'hybridation (1H à 60°C) des amplicons a été réalisée sur une membrane Isogen ® (Bioscience BV ... sous forme d'uncode octal et comparés aux données de la banque de spoligotypes SpolDB4 (Institut ...

 

[PDF] TABLA 8.1 _ GEL DE CORRIMIENTO

[PDF] from ula.ve

TNN - Identificación y diagnóstico de Actinomicetales … - saber.ula.ve... caontb. rivm. nl/Manuals/principl. html. Por su parte, Isogen Biosciences (http://www. isogen.nl) ofrece membranas con ADN inmobilizado de espaciadores, para utilizarse en pruebasde spoligotyping específicas para cepas de M. bovis/M. tuberculosis. ...

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Diversidade genética de estirpes de mycobacterium tuberculosis em pacientes com tuberculose nos distritos de Buzi e Manhiça, em Moçambique

[PDF] from utl.pt

CLS Mucavele - 2008 - repository.utl.pt... number of spoligotypes were identical to profiles found in the region. The data reported showedthat migration and HIV might be factors of dissemination of TB in Humans in Mozambique. Factors that can compromise the performance of Spoligotyping technique and other ...

[BOOK] Molecular Characterisation of Antibiotic Resistance in Mycobacterium Tuberculosis

[PDF] from ki.se

P Juréen - 2008 - diss.kib.ki.se... personnel (91). In order to overcome this, spoligotyping was evolved. This ... repeat) (91).MIRU-VNTR analyses copy-numbers of repeats, however, in contrast to spoligotyping,these have several sites scattered around the genome. Since ...

 

 

2007

 

Deletion-targeted multiplex PCR (DTM-PCR) for identification of Beijing/W genotypes of Mycobacterium tuberculosis

[PDF] from mvlab-fudan.cn

J Chen, AG Tsolaki, X Shen, X Jiang, J Mei… - Tuberculosis, 2007 - Elsevier... Spoligotyping was performed by using an Isogen Bioscience spoligotyping kit (Isogen Life Science ...Figure 3. Beijing/W Spoligotypes observed in 182 isolates with deletion RD105. Comparison of the multiplex PCR with spoligotyping revealed 100% sensitivity and specificity for ...

Contribution of spoligotyping to the characterization of the population structure of Mycobacterium tuberculosis isolates in Portugal

[TXT] from min-saude.pt

S David, DR Ribeiro, A Antunes, C Portugal… - Infection, Genetics and …, 2007 - Elsevier... Spoligotyping was carried out according to the manufacturer's instructions (Isogen BioscienceBV, Maarsen, Netherlands). The resulting spoligotypes were documented under a binary coderepresenting either a positive or negative hybridization result (n and o) and analyzed ...

Spoligotyping of< i> Mycobacterium tuberculosis</i> DNA from Archival Ziehl–Neelsen-stained sputum smears

N Suresh, J Arora, H Pant, T Rana… - Journal of microbiological …, 2007 - Elsevier... available kit, according to the instructions supplied by the manufacturer (Isogen Bioscience, BV ...a positive signal in spoligotyping, thus demonstrating the efficiency of spoligotyping in a ... analysisof DNA extracted from stored ZN slides identified tuberculosis spoligotypes in 41 ...

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Improvement of spoligotyping with additional spacer sequences for characterization of Mycobacterium bovis and M. caprae isolates from Spain

MT Javed, A Aranaz, L De Juan, J Bezos… - Tuberculosis, 2007 - Elsevier... using two membranes: a commercial membrane containing the 43 spacers described earlier23 (Isogen Bioscience BV ... Number of spoligotyping patterns of M. bovis and M. caprae.Thirty-one different spoligotypes were identified among the 308 M. bovis (26 patterns) and 88 ...

Utility of mycobacterial interspersed repetitive unit typing for differentiating Mycobacterium tuberculosis isolates in Wuhan, China

[HTML] from sgmjournals.org

H Han, F Wang, Y Xiao, Y Ren… - Journal of medical …, 2007 - Soc General Microbiol... To test the reproducibility of MIRU typing, we blindly typed 20 isolates in two independentexperiments. Spoligotyping. Spoligotyping was performed with a commercial spoligotyping kit(Isogen Bioscience), according to the instructions supplied by the manufacturer. ...

[PDF] … tuberculosis Beijingg family isolates from Hong Kong and comparisonn with 1S6110 restriction fragment lengthh polymorphism typing and spoligotyping

[PDF] from uva.nl K Kremer, BKY Au, PCW Yip, R Skuce, P Supply… - dare.uva.nl... with a commercial membranee containing the standard set of 43 spacer oligonucleotides (Isogen Bio- sciencee BV ... The usee of spacers 56, 57, 66, 67, and 68 in spoligotyping increased the ... patterns among the Beijing lineage strains in this study from three to five spoligotypes.. ...

 

Identification of Beijing lineage Mycobacterium tuberculosis with combined mycobacterial interspersed repetitive unit loci 26, 31, and ETR-A

[HTML] from nih.gov

PJ Chin, CC Chiu… - Journal of clinical microbiology, 2007 - Am Soc Microbiol... These isolates were characterized by IS6110 RFLP following standard protocols (12) andspoligotyping with a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands)(6). The characteristic ... All other spoligotypes are defined as non-Beijing genotypes. ...

Znaczenie metody spoligotyping w epidemiologicznych dochodzeniach gruźlicy

[PDF] from viamedica.pl

E Augustynowicz-Kopeć… - Pneumonologia i …, 2007 - pneumonologia.viamedica.pl... Autorzy dziękują Pani J. Scheenhart (Isogen Bioscience BV) za konsultację warunków repro ...IS6110 restriction fragment length polymor- phism patterns and spoligotypes determined by ...Spoligotyping of Myco- bacterium tuberculosis isolates from patients with pulmo- nary ...

[HTML] Dedicated decontamination: A necessity to prevent cross contamination in high throughput mycobacteriology laboratories

[HTML] from jpgmonline.comC Rodrigues, D Almeida, S Shenai, N Goyal… - Indian Journal of …, 2007 - Medknow

... Spoligotyping Spoligotyping was performed with a commercially available kit (Isogen Bioscience). ...Remaining two isolates grown from smear positive specimens had different spoligotypes hence were ... Since spoligotyping is known to be less refractory for differentiation of M ...

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Special Article-Dedicated decontamination: A necessity to prevent cross contamination in high throughput mycobacteriology laboratories

[PDF] from bioline.org.br

C Rodrigues, D Almeida, S Shenai… - Indian Journal of …, 2007 - bioline.org.br... Spoligotyping was performed with a commercially available kit (Isogen Bioscience). ... Remainingtwo isolates grown from smear positive specimens had different spoligotypes hence were ... Sincespoligotyping is known to be less refractory for differentiation of M. tuberculosis, we ...

 

[HTML] Characterisation of mycobacteria isolated from slaughter cattle in pastoral regions of Uganda

[HTML] from biomedcentral.com

J Oloya, R Kazwala, A Lund… - BMC …, 2007 - biomedcentral.com... in mid-central and mid-western areas of Uganda and detected new and specific spoligotypesnot reported ... Cultures belonging to the M. tuberculosis complex were submitted for furtherdifferentiation by Spoligotyping [20] as described by the producer (Isogen, Life science 

Genetic diversity of Mycobacterium tuberculosis isolates at the Military Medical Academy in Ankara, Turkey

O Kisa, A Albay, O Baylan, E Tozkoparan… - Research in …, 2007 - Elsevier

... Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen, The

Netherlands) according to the ... 2.6. Comparison of spoligotypes with the international spoligotype

database. Spoligotyping results were entered in a binary format and octal code on Excel ...

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Evolution of the extensively drug-resistant F15/LAM4/KZN strain of Mycobacterium tuberculosis in KwaZulu-Natal, South Africa

[HTML] from oxfordjournals.org

M Pillay… - Clinical infectious diseases, 2007 - cid.oxfordjournals.org

... Spoligotyping. Spoligotyping was performed using a kit from Isogen-Life Science in accordance with the manufacturer's instructions. The presence or absence of 43 spacers in the direct-repeat region of isolates of M. tuberculosis ...

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[HTML] Longitudinal assessment of an ELISPOT test for Mycobacterium tuberculosis infection

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PC Hill, RH Brookes, A Fox, D Jackson-Sillah… - PLoS medicine, 2007 - dx.plos.org

... We performed spoligotyping using membranes from Isogen Biosciences (http://www.isogen- lifescience.com), as previously described [18] and analysed the results with software that we designed using Matlab software (MathWorks, http://www.mathworks.com). ...

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[HTML] M. tuberculosis genotypic diversity and drug susceptibility pattern in HIV-infected and non-HIV-infected patients in northern Tanzania

[HTML] from biomedcentral.comGS Kibiki, B Mulder, WMV Dolmans… - BMC …, 2007 - biomedcentral.com... with samples which were INH-resistant, contained phenotypic rifampicin-resistant strains, or hadselected spoligotypes; phenotypic rifampicin ... Spoligotyping. ... 1 U/μl). Amplification and hybridizationwere performed according to the manufacturer's manual (Isogen Bioscience BV ...

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Outbreak of Mycobacterium bovis infection in a wild animal park

SM Schmidbauer, P Wohlsein, G Kirpal… - Veterinary …, 2007 - veterinaryrecord.bmj.com... and subtype of the MTC agent, 17 isolates from various organs were spoligotyped (Kamerbeekand ... FIG 2: Spoligotyping pattern of 17 mycobacterial isolates from various organs from seven ...in wild animals in Germany and in these cases the spoligotypes normally correspond to ...

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Molecular investigation of recurrent tuberculosis in patients from Rwanda

AN Umubyeyi, IC Shamputa… - … of Tuberculosis and …, 2007 - ingentaconnect.com... DNA fingerprintingtechniques Spoligotyping was performed using a commercial kit (Isogen

Bioscience BV ... 4 of the 12 previously cured MDR-TB patients showed different spoligotypes,suggesting re ... of their recurrence (Figures 1 and 2). In contrast, the spoligotyping pat- terns of ...

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[HTML] Using ELISPOT to expose false positive skin test conversion in tuberculosis contacts

[HTML] from plos.org

PC Hill, DJ Jeffries, RH Brookes, A Fox… - PLoS One, 2007 - dx.plos.org... spectrophotometry. We performed Spoligotyping using membranes (IsogenBiosciences), as previously described,[14] scanned the results and analysed themwith software designed in Matlab. Data management and analysis. ...

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[PDF] Usefulness of molecular techniques to identify ongoing tuberculosis transmission in Saudi Arabia

[PDF] from smj.org.sa

SA Al-Hajoj, VK Mohammed… - Saudi medical journal, 2007 - smj.org.sa... The labeled PCR product was used as a probe to hybridize with 43 synthetic spacer oligonucleotides attached to a carrier membrane (Isogen Bioscience BV,Maarssen, TheNetherlands). The 2 isolates were identical by both RFLP and spoligotyping methods Figure ...

Molecular clues of a microepidemy among homeless tuberculosis patients in Budapest due to a new and local Mycobacterium tuberculosis clade

C Ködmön, S Niemann, MC Gutierrez… - Infection, Genetics and …, 2007 - Elsevier... Spoligotyping was performed with a commercially available kit (Isogen BioscienceBV, Maarssen, The Netherlands) according to the instructions of the manufacturer(Kremer et al., 1999). Briefly, genomic mycobacterial DNA ...

Discovery of a novel Mycobacterium tuberculosis lineage that is a major cause of tuberculosis in Rio de Janeiro, Brazil

[HTML] from nih.govLCO Lazzarini, RC Huard, NL Boechat… - Journal of clinical …, 2007 - Am Soc Microbiol... Spoligotyping.All M. tuberculosis strains were spoligotyped using a commercial kit (IsogenBioscience BV ... as an octal code (9). The person who performed the spoligotyping was blindedto ... information on the shared-type distributions of M. tuberculosis spoligotypes worldwide (N ...

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Genotype and phenotype relationships and transmission analysis of drug-resistant tuberculosis in Singapore

YJ Sun, ASG Lee, SY Wong… - … of Tuberculosis and …, 2007 - ingentaconnect.com

... Spoligotyping Spoligotyping was performed using a commercial kit obtained from Isogen

Bioscience BV ... as previously described.21,22 IS6110 RFLP patterns and/or MIRU-VNTR patterns were used to determine genotypes for a few isolates with indeterminate spoligotypes. ...

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Evidence of 'amplifier effect'in pulmonary multidrug-resistant tuberculosis: report of three casesA Umubyeyi, IC Shamputa, L Rigouts… - International Journal of …, 2007 - Elsevier... Spoligotyping. Spoligotyping was performed with a commercial kit (Isogen Bioscience BV, Maarssen, The Netherlands) by the previously described method. 10 Briefly, 4 μl of the heat-killed bacterial suspension from each sample ...

[PDF] STUDY OF ISONIAZID-RESISTANT TUBERCULOSIS: MYCOBACTERIAL GENOTYPIC CHARACTERIZATION AND CLINICAL FEATURES IN HUMAN[PDF] from usc.edu

P Escalante - 2007 - digitallibrary.usc.edu... BioImage Whole Band Analyzer™, version 3.2 software (BioImage, Ann Arbor, MI). Spoligotypingwas performed using the Isogen™ kit (Isogen, Bioscience, BV Maarseen, Netherlands) for allisolates according to the manufacturer instructions. In addition, all ...

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[BOOK] Study of isoniazid-resistant tuberculosis: Mycobacterial genotypic characterization and clinical features in human subjects

P Escalante - 2007 - books.google.com software (BioImage, Ann Arbor, MI). Spoligotyping was performed using the Isogen kit (Isogen, Bioscience, BV Maarseen, Netherlands) for all isolates according to the manufacturer instructions. In addition, all isolates also ...

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First insight into the population structure of Mycobacterium tuberculosis in Saudi Arabia

[HTML] from nih.govSAM Al-Hajoj, T Zozio, F Al-Rabiah… - Journal of clinical …, 2007 - Am Soc Microbiol... to inter-DR spacer oligonucleotides covalently bound to a membrane (Isogen Life Science ... All spoligotypes were converted into octal format within Excel spreadsheets (8) and ... The individual spoligotyping patterns were compared with an updated SpolDB4 database named ...

Transmission of< i> Mycobacterium tuberculosis</i> in a Family Proved by Genotyping

CY Yang, R Jou, PC Chuang, JT Chang… - Journal of the Formosan …, 2007 - Elsevier Spoligotyping Spoligotyping is a polymerase chain reaction (PCR)-based analysis that uses commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands). ... The re-solved spoligotypes were M. tuberculosis complex strain-specific. ...

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[PDF] Molecular characterization of Mycobacterium tuberculosis isolates from Izmir, Turkey

[PDF] from microbiologica.net

C Bicmen, N Esen, EA Graviss… - … -Quarterly Journal of …, 2007 - microbiologica.net. were analyzed with the BioImage (Ann Arbor, Mich.,USA) Whole Band Analyzer, version 3.4.2.Spoligotyping was per- formed with a commercially available kit (Isogen Bioscience BV, Maarssen,

The Netherlands) in accordance with the manufacturer's recom- mendations. ...

Instability of IS6110 patterns in multidrug-resistant strains of Mycobacterium tuberculosis

[HTML] from nih.gov

P Farnia, MR Masjedi, B Nasiri… - Epidemiology and …, 2007 - Cambridge Univ Press [12] with a commercially avail- able kit (Isogen Bioscience BV ... Stability of M. tuberculosis IS6110restriction fragment length polymorphism patterns and spoligotypes determined by ... expandingclones of M. tuberculosis and their global distribution assessed by spoligotyping in an …

Identification of unique genetic markers in Rv0927c among< i> Mycobacterium tuberculosis</i> W-Beijing strainsX Jiang, Y Zhang, W Zhang, F Gao, J Jin… - Microbes and …, 2007 - Elsevier... SDS) at 60 °C and then incubated in 1:4000 diluted streptavidin–peroxidase conjugate (IsogenBioscience) for ... This finding, which showed a spoligotyping pattern with hybridization only to thespacer 35 to 43 ... 1. Spoligotypes of the 56 M. tuberculosis clinical isolates from China. ...

Laboratory diagnosis of pulmonary tuberculosis in TB and HIV endemic settings and the contribution of real time PCR for M. tuberculosis in bronchoalveolar lavage …

[PDF] from ru.nl

GS Kibiki, B Mulder… - Tropical Medicine & …, 2007 - Wiley Online Library

... Spoligotyping patterns were analysed with Bionumerics 4.0 (Applied Maths, St-Martin-Latem,

Belgium). ... strains in 25 of the 28 BAL samples with positive MTB culture could be spoligotyped.

In all 25, the spoligotypes indicated that the isolates belonged to M. tuberculosis complex ...

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[PDF] MYCOBACTERIOSIS IN WILD BOAR: RESULTS OF 2000-2006 ACTIVITY IN NORTH-WESTERN ITALY

[PDF] from vet-alfort.fr

A Dondo, S Zoppi, F Rossi, L Chiavacci, A Barbaro… - aeema.vet-alfort.fr ... 37 a PCR based reverse line blot method covalently (Isogen, Netherland). M. bovis strains isolated were further characterized by VNTR typing (ETR A, B, C, D, E) according to Frothingham[Frothingham et al., 1998]. The strains unidentified by Spoligotyping were processed by a ...

Molecular epidemiology and prevalence of mutations conferring rifampicin and isoniazid resistance in Mycobacterium tuberculosis strains from the southern Ukraine

VV Nikolayevskyy, TJ Brown… - Clinical …, 2007 - Wiley Online Library[23] using commercially available membranes (Isogen, Maarssen, The ... binding to the corresponding targets, but an association between these specific spoligotypes and mutations in ... Spoligotyping results demonstrated a relative diversity in the genetic structure of M. tuberculosis ...

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Discrepant Results Between Pyrazinamide Susceptibility Testing by the Reference BACTEC 460TB Method and pncA DNA Sequencing in Patients Infected With …

J Dormandy, A Somoskovi, BN Kreiswirth… - Chest, 2007 - chestjournal.chestpubs.org... Spoligotyping, a polymerase chain reaction-based genotyping method that detects 43 known spacer sequences that are interspersed between the direct repeats in the genomic repeat regionof M tuberculosis, was performed with a commercially available kit (Isogen Bioscience ...

Új Mycobacterium tuberculosis törzs okozta mikroepidémia budapesti hajléktalanokban C Ködmön, J Lukács, S Dávid, I Vadász… - Orvosi Hetilap, 2007 - akademiai.com A spoligotyping kevésbé érzékeny, mint az IS6110-RFLP fin- gerprint módszer, de kitûnôen elkülöníthetôk vele a világszer- te ... identifikáltuk [5, 6]. A mycobacteriális DNS kinyerését és a spoligotipizálást ke- reskedelmi forgalomban kapható teszttel (Isogen Bioscience BV ...

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2006

 

A case of urinary tuberculosis due toMycobacterium bovissubspeciescaprae

V Sintchenko, P Jelfs, M Dally… - Pathology-Journal of …, 2006 - journals.lww.com... Spoligotyping (Isogen Bioscience, The Netherlands), which involves PCR amplification of the MTBC direct repeat ... of reference laboratories to differentiate members of the MTBC by spoligotyping, 11 based on ... Several spoligotypes have been recognised among M. bovis subsp. ...

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… tandem-repeat genotyping of Mycobacterium bovis and comparison of the method with IS6110 restriction fragment length polymorphism analysis and spoligotyping

[HTML] from nih.gov

C Allix, K Walravens, C Saegerman… - Journal of clinical …, 2006 - Am Soc Microbiol ... PCR products were detected by hybridization onto a spoligotyping membrane (Isogen Bioscience BV ... IS6110 RFLP, spoligotyping, and MIRU-VNTR patterns of isolates obtained from animals ... based on MIRU-VNTR types and IS6110 fingerprints and spoligotypes was evaluated ...

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Spoligotyping of Mycobacterium tuberculosis isolates from Pakistan reveals predominance of Central Asian Strain 1 and Beijing isolates

[HTML] from nih.gov

Z Hasan, M Tanveer, A Kanji, Q Hasan… - Journal of clinical …, 2006 - Am Soc Microbiol positive. Spoligotyping.All 314 isolates were spoligotyped and analyzed (Fig. 1). A total of 197 (63%) isolates were grouped into 22 different clusters, while119 (37%) isolates had unique spoligotypes. Previously ...

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Molecular epidemiology of multidrug-resistant Mycobacterium bovis isolates with the samespoligotyping profile as isolates from animals

[HTML] from nih.gov

B Romero, A Aranaz, L de Juan… - Journal of clinical …, 2006 - Am Soc Microbiol... Spoligotyping was performed on a commercial membrane (Isogen Bioscience BV, Maarssen,The ... of techniques is required for appropriate discrimination, even for those spoligotypes that have a ... Furthermore, the design of a new spoligotyping membrane that complements the ...

< i> rpoB</i> gene sequencing and spoligotyping of multidrug-resistant< i> Mycobacterium tuberculosis</i> isolates from India

N Suresh, UB Singh, J Arora, H Pant… - Infection, Genetics and …, 2006 - Elsevier... to the AIIMS hospital, 149 MDR TB strains and 38 drug-susceptible strains were sequenced to identify mutations associated with RIF r in the rpoB gene and spoligotyped. 2.2. ... Spoligotyping. A total of 80 distinct spoligotypes were obtained from 149 MDR M. tuberculosis isolates. ...

Usefulness of restriction fragment length polymorphism and spoligotyping for epidemiological studies of< i> Mycobacterium bovis</i> in Madagascar: Description of …

V Rasolofo Razanamparany, R Quirin… - Veterinary …, 2006 - Elsevier

... containing 43 oligonucleotides derived from spacer DNA sequences (obtained from Isogen Bioscience BV, Utrecht, The Netherlands). PCR and hybridization were performed as described by Kamerbeek et al. (1997). Spoligotyping patterns or spoligotypes were confirmed by ...

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[HTML] Variantes genéticas de Mycobacterium tuberculosis aisladas de pacientes de la Xª Región de Chile

[HTML] from scielo.cl

M Mancilla E, A Martínez H… - Revista chilena de …, 2006 - SciELO Chile... A significant number of spoligotypes were identical to profiles found in Brazil followed by Argentina and Spain. ... utilizando los partidores biotinilados DRa (5'-GGTTTTGGGTCTGACGAC) y DRb (5'- CCGAGA GGGGACGGAAAC), incluidos en el sistema de spoligotyping (ISOGEN. ...

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Mycobacterium africanum elicits an attenuated T cell response to early secreted antigenic target, 6 kDa, in patients with tuberculosis and their household contacts

[HTML] from oxfordjournals.org

BC de Jong, PC Hill, RH Brookes… - Journal of Infectious …, 2006 - jid.oxfordjournals.org

... were assessed by spectrophotometry (Eppendorf BioPhotometer). Spoligotypingwas performed using membranes (Isogen Biosciences), in accordance withstandardized methods [19]. Spoligotyping results were scanned and ...

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rpoB gene mutations and molecular characterization of rifampin-resistant Mycobacterium tuberculosis isolates from Shandong Province, China

[HTML] from nih.gov

X Ma, H Wang, Y Deng, Z Liu, Y Xu… - Journal of clinical …, 2006 - Am Soc Microbiol... (20), with data analyzed by Whole Band Analysis software (version 3.2; BioImage,Ann Arbor, MI). Spoligotyping was carried out by using commercial kits from Isogen Bioscience BV (Maarssen, The Netherlands). Mycobacterial ...

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[HTML] Tuberculosis associated with Mycobacterium tuberculosis Beijing and non-Beijing genotypes: a clinical and immunological comparison

[HTML] from biomedcentral.com

YJ Sun, TK Lim, A Ong, B Ho… - BMC infectious …, 2006 - biomedcentral.com... Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen, theNetherlands ... The remaining one isolate (N07) was not detected by spoligotyping, but MIRU-VNTR ...Based on spoligotypes and/or MIRU-VNTR patterns, the remaining 20 isolates were ...

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Prevalence of Haarlem I and Beijing types of Mycobacterium tuberculosis strains in Iranian and Afghan MDR-TB patients

[PDF] from mrc.ac.ir

P Farnia, MR Masjedi, M Mirsaeidi, F Mohammadi… - Journal of Infection, 2006 - Elsevier... Spoligotyping. Spoligotyping was performed as described by Kamerbeek et al. 5 with acommercially available kit, according to the instructions supplied by the manufacturer (IsogenBioscience BV, Maarsen, The Netherlands). ... Distribution of the 263 MDR-TB spoligotypes. ...

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Molecular epidemiology of drug-resistant tuberculosis in Hungary

[HTML] from nih.gov

C Ködmön, S Niemann, J Lukács… - Journal of clinical …, 2006 - Am Soc Microbiol... described previously (3, 14, 17). Spoligotyping was performed with a commerciallyavailable kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to theinstructions of the manufacturer (10). The IS6110 fingerprint and ...

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Tuberculosis in mandrills at the Lisbon zooA Amado, T Albuquerque, A Gonçalves… - Veterinary …, 2006 - veterinaryrecord.bmj.com... In order to investigate the infection source, spoligotyping was performed using conventional 43oligonucleotide Isogen membranes as described by Kamerbeek and others (1997). The twoisolates shared the same spoligotype described as type 237 (Anon 2006). ...

[HTML] Molecular characterisation of Mycobacterium tuberculosis isolates in the First National Survey of Anti-tuberculosis Drug Resistance from Venezuela

[HTML] from biomedcentral.com

L Aristimuño, R Armengol, A Cebollada… - BMC …, 2006 - biomedcentral.com... to a set of 43 oligonucleotides covalently bound to the spoligo-membrane (Isogen BiosciencesBV ... of Mycobacterium tuberculosis and their global distribution assessed by spoligotyping in anInternational study Global distribution of Mycobacterium tuberculosis spoligotypes. ...

 

[PDF] 2. Lipoarabinomannans—structurally diverse and functionally enigmatic macroamphiphiles of mycobacteria and related actinomycetes•

[PDF] from pasteur.ac.ir

I Sutcliffe, T Matsumoto, H Ano, T Nagai, K Danno… - 2006 - journals2005.pasteur.ac.ir

... Spoligotyping The clones of M. tuberculosis with less than five IS6110 RFLP bands were subjectedto the second typing, spoligotyping. Spoligotyping membranes were purchased from Isogenbioscience BV (Biltho- ven, The Netherlands). The procedures were ...

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Beijing genotype of Mycobacterium tuberculosis is significantly associated with human immunodeficiency virus infection and multidrug resistance in cases of …

[HTML] from nih.gov

M Caws, G Thwaites, K Stepniewska… - Journal of clinical …, 2006 - Am Soc Microbiol... Spoligotyping.Spoligotyping of isolates was performed according to the standard protocol (6).Briefly, DNA ... Dra (biotin labeled) and DRb, hybridized to the spoligotype membrane (IsogenBioscience BV ... Spoligotypes were available for isolates from 35 HIV-positive patients (Fig. ...

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The most prevalent Mycobacterium tuberculosis superfamilies among Iranian and Afghan TB cases

[PDF] from mrc.ac.ir

AA Velayati, P Farnia, M Mirsaeidi… - … journal of infectious …, 2006 - informahealthcare.com... [7] with a commercially available kit, according to the instructions supplied by the manufacturer(Isogen Bioscience BV, Maarsen, The Netherlands). ... Spoligotyping ... Figure 1. Shows thepredominant spoligotypes which are prevalent among Iranian and Afghan-born immigrants. ...

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Extensively drug-resistant tuberculosis: 2 years of surveillance in Iran

[HTML] from oxfordjournals.org

MR Masjedi, P Farnia, S Sorooch… - Clinical infectious …, 2006 - cid.oxfordjournals.org[13] with a commercially available kit (Isogen Bioscience). ... On the basis of spoligotyping results, these strains were determined to belong to the East African Indian 3 M. tuberculosis superfamily.Table 1. Table 1 ... Distribution of the 12 XDR TB spoligotypes. ...

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[HTML] Predominance of ancestral lineages of Mycobacterium tuberculosis in India

[HTML] from 9med.net

MC Gutierrez, N Ahmed, E Willery… - Emerg Infect …, 2006 - journal.9med.net... Spoligotyping was performed by using a commercial kit (Isogen Bioscience BV, Maarsen, the Netherlands) according to ... was built based on spoligotypes alone or on a combination of VNTR and spoligotypes, although the resolution was lower when spoligotyping was used ...

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High resolution typing system for pathogenic Mycobaterium tuberculosum

PS Keim, RS Spurgiesz, JM Schupp - US Patent 7,026,467, 2006 - Google Patents

... 7:390-6. 2001. Sola, C. et al., "Mycobacterium tuberculosis phylogeny reconstruction based on combined numerical analysis with IS1081, IS6110, VNTR, and DR-based spoligotyping sug- gests the existence of two new phlyogeographical clades". J Molecr Ev. 53:680-9. 2001. ...

Mycobacterium tuberculosis genotypes and their relationships with clinical and immunological phenotypes in Singapore

[PDF] from 137.132.14.55

SUN YONGJIANG - 2006 - 137.132.14.55

... Furthermore, this method is highly reproducible and easy to perform (Kremer et al., 1999); particularly spoligotypes can be digitized into binary or octal code formats (Dale et al., 2001) which greatly facilitate the management and interlaboratory comparison of spoligotyping data. ...

Recent transmission of tuberculosis involving retired patients

PI Cafrune, LW Riley, LG Possuelo, ARM Valim… - Journal of Infection, 2006 - Elsevier... Spoligotyping. Spoligotyping was performed as described by Kamerbeek et al. 19 ...

probed. Hybridization was performed with a commercial kit, according to the manufacturer instructions (Isogen Biosciences BV, The Netherlands). ...

 

[PDF] INFORME FINAL DE PROYECTO DE INVESTIGACIÓN

[PDF] from ins.gob.pe

EN MULTIDROGORESISTENCIA… - bvs.ins.gob.pe

... Salud e interpretadas en el software ALFWin TM Sequence Analyser 2.0 1998. Ensayode genotipificación mediante Spoligotyping Fue usado un kit comercialmentedisponible (Isogen Bioscience BV, Maarssen, The Netherlands ...

Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis

[PDF] from vetres-archive.org

A Aranaz, L De Juan, J Bezos, J Álvarez… - Veterinary …, 2006 - vetres.org

... Thirty-six M. bovis isolates were char- acterised by spoligotyping and MIRU- VNTR: the 23 M ... The biotin-labelled amplified product was hybridised onto a membrane (Isogen Bio- science BV,

Maarssen ... Four spoligotypes were found, and three of them, numbered spb-7, spb-9 ...

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INTÉRÊT DU TYPAGE MOLÉCULAIRE POUR L'ÉPIDÉMIOLOGIE DE LA TUBERCULOSE EN LIMOUSIN (1998–2006)

[HTML] from em-consulte.com

H Bezanahary, MC Baclet, C Sola… - Médecine et Maladies …, 2008 - Elsevier... Keywords: MIRU15 typing; Mycobacterium tuberculosis; Spoligotyping. ... L'hybridation (1H à 60°C) des amplicons a été réalisée sur une membrane Isogen ® (Bioscience BV ... sous forme d'uncode octal et comparés aux données de la banque de spoligotypes SpolDB4 (Institut ...

… TANDEM-REPEAT GENOTYPING OF MYCOBACTERIUM BOVIS AND COMPARISON OF THE METHOD WITH IS6110 RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS AND SPOLIGOTYPING

[HTML] from nih.gov

C Allix, K Walravens, C Saegerman… - Journal of clinical …, 2006 - Am Soc Microbiol... PCR products were detected by hybridization onto a spoligotyping membrane (Isogen BioscienceBV ... IS6110 RFLP, spoligotyping, and MIRU-VNTR patterns of isolates obtained from animals ... based on MIRU-VNTR types and IS6110 fingerprints and spoligotypes was evaluated ...

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A CASE OF URINARY TUBERCULOSIS DUE TOMYCOBACTERIUM BOVISSUBSPECIESCAPRAE

V Sintchenko, P Jelfs, M Dally… - Pathology-Journal of …, 2006 - journals.lww.com... Spoligotyping (Isogen Bioscience, The Netherlands), which involves PCR amplification of the MTBC direct repeat ... of reference laboratories to differentiate members of the MTBC by spoligotyping, 11 based on ... Several spoligotypes have been recognised among M. bovis subsp. ...

SPOLIGOTYPING OF MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM PAKISTAN REVEALS PREDOMINANCE OF CENTRAL ASIAN STRAIN 1 AND BEIJING ISOLATES

[HTML] from nih.gov

Z Hasan, M Tanveer, A Kanji, Q Hasan… - Journal of clinical …, 2006 - Am Soc Microbiol

... positive. Spoligotyping.All 314 isolates were spoligotyped and analyzed (Fig.1). A total of 197 (63%) isolates were grouped into 22 different clusters, while119 (37%) isolates had unique spoligotypes. Previously ...

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MOLECULAR EPIDEMIOLOGY OF MULTIDRUG-RESISTANT MYCOBACTERIUM BOVIS ISOLATES WITH THE SAMESPOLIGOTYPING PROFILE AS ISOLATES FROM ANIMALS

[HTML] from nih.gov

B Romero, A Aranaz, L de Juan… - Journal of clinical …, 2006 - Am Soc Microbiol... Spoligotyping was performed on a commercial membrane (Isogen Bioscience BV, Maarssen,The ... of techniques is required for appropriate discrimination, even for those spoligotypes that havea ... Furthermore, the design of a new spoligotyping membrane that complements the ...

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< I> RPOB</I> GENE SEQUENCING AND SPOLIGOTYPING OF MULTIDRUG-RESISTANT< I> MYCOBACTERIUM TUBERCULOSIS</I> ISOLATES FROM INDIA

N Suresh, UB Singh, J Arora, H Pant… - Infection, Genetics and …, 2006 - Elsevier

... to the AIIMS hospital, 149 MDR TB strains and 38 drug-susceptible strains were sequenced to

identify mutations associated with RIF r in the rpoB gene and spoligotyped. 2.2. ... Spoligotyping.

A total of 80 distinct spoligotypes were obtained from 149 MDR M. tuberculosis isolates. ...

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2005

[HTML] Spoligotyping of Mycobacterium bovis isolates found in Manitoba

[HTML] from nih.gov

C Lutze-Wallace, C Turcotte… - Canadian journal of …, 2005 - ncbi.nlm.nih.gov... Table I. Table I Summary of spoligotyped isolates by year, species, and location. ... types are 656573377603600 (MB-1) and 656473377603600 (MB-2). These spoligotypes have not … Spoligotyping is particularly useful for differentiating isolates harbouring only a single copy of ...

Characterization of Mycobacterium tuberculosis complex isolates from Greek patients with sarcoidosis by spoligotyping

[HTML] from nih.gov

M Gazouli, J Ikonomopoulos… - Journal of clinical …, 2005 - Am Soc Microbiol... hybridization with membrane-bound spacer oligonucleotides and a spoligotyping kit (ISOGEN Bioscience, Maarsen ... The spoligotyping results were entered in a binary format as Excel spreadsheets ... All of the identified spoligotypes have been submitted to the World Spoligotype ...

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… Mycobacterium tuberculosis Beijing family isolates from Hong Kong and comparison with IS6110 restriction fragment length polymorphism typing and spoligotyping

[HTML] from nih.gov

K Kremer, BKY Au, PCW Yip, R Skuce… - Journal of clinical …, 2005 - Am Soc Microbiol... In addition to spoligotyping with a commercial membrane containing the standard set of 43 spaceroligonucleotides (Isogen Bioscience BV, Maarssen, The ... (35), who also subjected Beijing strains to spoligotyping with novel spacers, at least seven spoligotypes can be ...

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Characterization of clonal complexity in tuberculosis by mycobacterial interspersed repetitive unit-variable-number tandem repeat typing

[HTML] from nih.gov

DG de Viedma, NA Rodriguez, S Andrés… - Journal of clinical …, 2005 - Am Soc Microbiol... Spoligotyping.Spoligotyping was performed using primers of a spoligotyping kit (Isogen, The Netherlands ... with the loci showing different alleles within a box) and spoligotypes for the ... The two clones with different MIRU types were indistinguishable by spoligotyping and RFLP (Fig ...

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Discrimination of isolates of Mycobacterium bovis in Northern Ireland on the basis of variable numbers of tandem repeats (VNTRs)

RA Skuce, SW McDowell, TR Mallon… - Veterinary …, 2005 - veterinaryrecord.bmj.com

... The isolates were spoligotyped according to the instructions of the manu- facturer (Isogen). ... the

inclusion of more than one isolate per herd (VNTR D=0·90, spoligotyping D=0 ... The difference

between years in the prevalence of VNTR pro- files and spoligotypes was not statistically ...

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[HTML] Molecular genotyping of Mycobacterium tuberculosis isolates from Turkey

[HTML] from biomedsearch.com

B Gencer… - Am J Infect Dis, 2005 - biomedsearch.com

... Of the 374 isolates spoligotyped, 87 (23%) displayed unique spoligotypes and 287 (77 ...Spoligotyping: Spoligotyping was performed using a commercial kit (Isogen Bioscience BV, Maarssen ... Spoligotyping: Spoligotypes were obtained for 374 isolates (Table 2): 87 isolates (23 ...Cited by 5 - Related articles - Cached - All 6 versions - Import into BibTeX

[CITATION] Epidemiologische Studie bei 109 Tuberkulosefällen im Regierungsbezirk Oberfalz basierend auf Spoligotyping

B Werner, C Ellermeier, L Naumann… - Pneumologie, 2005

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Inter-laboratory validation of PCR-based detection of Mycobacterium tuberculosis in formalin-fixed, paraffin-embedded tissues

[PDF] from charite.de

C Schewe, T Goldmann, M Grosser, A Zink… - Virchows Archiv, 2005 - Springer... All samples with positive amplification of IS6110 were subjected to further analysis by spoligotyping. The method was performed as described previously [22, 32, 44]. We used a commercial Spoligotyping Kit (Isogen, Maarsen, The Netherlands, http://www.isogen.nl/). ...

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[CITATION] Case-Report: Lungentuberkulose einer MTA–Nachweis von 3 Neuinfektionen durchSpoligotyping B Werner, C Ellermeier, L Naumann… - Pneumologie, 2005

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Molecular characterization of< i> Mycobacterium tuberculosis</i> isolates in a region of Brazil with a high incidence of tuberculosis

S Borsuk, MM Dellagostin, SG Madeira, C Lima… - Microbes and …, 2005 - Elsevier

... Hybridization of the PCR product to the spoligo-membrane (Isogen Life Sciences) was ... The strains

clustered by spoligotyping were analyzed by IS6110-RFLP to confirm any ... The spoligotypes were

compared to SpoligoDatabase3 (http://www.pasteur-guadeloupe.fr/tb/spoldb3 ...

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S< i> 6110</i> DNA fingerprinting analysis of individually separated colonies of< i> Mycobacterium tuberculosis</i>

T Matsumoto, H Ano, T Nagai, K Danno… - Tuberculosis, 2005 - Elsevier

... to the second typing, spoligotyping. Spoligotyping membranes were purchasedfrom Isogen bioscience BV (Bilthoven, The Netherlands). The procedures werecarried out according to the manufacturer's instructions. Results. ...

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Distribution of the Beijing family genotypes of Mycobacterium tuberculosis in Taiwan

[HTML] from nih.govR Jou, CY Chiang… - Journal of clinical microbiology, 2005 - Am Soc Microbiol... Genotyping method.Spoligotyping was used to identify and differentiate the M. tuberculosiscomplex isolates in ... A commercially available kit (Isogen Bioscience BV, Maarssen, The

Netherlands) was used as ... The spoligotypes resolved were M. tuberculosis complex strain specific ...

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Characterization of Mycobacterium caprae isolates from Europe by mycobacterial interspersed repetitive unit genotyping

[HTML] from nih.gov

WM Prodinger, A Brandstätter… - Journal of clinical …, 2005 - Am Soc Microbiol

... Spoligotyping and IS6110 RFLP analysis.Spoligotyping was carried out as described previously (23) by using membranes (Isogen Bioscience BV, Maarsen, The Netherlands). The octal codes for spoligotypes were determined as proposed previously (14). ...

Novos dados sobre os Spoligotypes de estirpes do complexo Mycobacterium tuberculosis isoladas no Hospital Fernando Fonseca (Amadora-Sintra, Portugal)

[TXT] from min-saude.pt

S Davis, V Barros, C Portugal… - Rev Port …, 2005 - repositorio.hff.min-saude.pt... out in accordance with the recommendations of the mem- brane Kit manufacturer (IsogenBioscience BV ... contributed the most in this field, seeing that it allows the Spoligotypes to be ...Spoligotyping make it easier to differentiate between the species of the M. tuberculosis complex ...

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Efficient discrimination within a Corynebacterium diphtheriae epidemic clonal group by a novel macroarray-based method

[HTML] from nih.gov

I Mokrousov, O Narvskaya… - Journal of clinical …, 2005 - Am Soc Microbiol .. in parallel slots (channels) by using an MN45 miniblotter apparatus (Isogen Biosciences,Maarssen ... both ribotypes of the epidemic clone, while the other 33 spoligotypes included strains ...had unique profiles (Table 2). The discriminatory power of the spoligotyping assessed with ...

Place du spoligotypage lors d'une enquête épidémiologique devant un cas de suspicion de tuberculose pulmonaire nosocomiale F Jauréguy, MC Gutierez, CJ Marie, C Poirier… - Pathologie Biologie, 2005 - Elsevier ... Keywords: Nosocomial tuberculosis; Epidemiology; Spoligotyping. ... inter-DR ou spacers ont été synthétisées et immobilisées sur une membrane de nylon commercialisée (Isogen Bioscience BV, BT ... Les résultats des différents spoligotypes obtenus sont présentés dans la Fig. ...

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[CITATION] 波兰肺结核患者耐药结核分枝杆菌分子流行病学研究A Sajduda, A Brzostek, M PopXawska, N Rastogi… - 结核与肺部疾病杂志, 2005

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[HTML] SPOLIGOTYPING OF MYCOBACTERIUM BOVIS ISOLATES FOUND IN MANITOBA

[HTML] from nih.govC Lutze-Wallace, C Turcotte… - Canadian journal of …, 2005 - ncbi.nlm.nih.gov... Table I. Table I Summary of spoligotyped isolates by year, species, and location. ... types are656573377603600 (MB-1) and 656473377603600 (MB-2). These spoligotypes have not ...

Spoligotyping is particularly useful for differentiating isolates harbouring only a single copy of ...

CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX ISOLATES FROM GREEK PATIENTS WITH SARCOIDOSIS BY SPOLIGOTYPING

[HTML] from nih.gov

M Gazouli, J Ikonomopoulos… - Journal of clinical …, 2005 - Am Soc Microbiol... hybridization with membrane-bound spacer oligonucleotides and a spoligotyping kit (ISOGENBioscience, Maarsen ... The spoligotyping results were entered in a binary format as Excelspreadsheets ... All of the identified spoligotypes have been submitted to the World Spoligotype ...

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… MYCOBACTERIUM TUBERCULOSIS BEIJING FAMILY ISOLATES FROM HONG KONG AND COMPARISON WITH IS6110 RESTRICTION FRAGMENT LENGTH POLYMORPHISM TYPING AND SPOLIGOTYPING

[HTML] from nih.gov

K Kremer, BKY Au, PCW Yip, R Skuce… - Journal of clinical …, 2005 - Am Soc Microbiol

... In addition to spoligotyping with a commercial membrane containing the standard set of 43 spacer

oligonucleotides (Isogen Bioscience BV, Maarssen, The ... (35), who also subjected Beijing strains

to spoligotyping with novel spacers, at least seven spoligotypes can be ...

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RECHERCHE DE MARQUEURS GÉNÉTIQUES DE SOUCHES DE MYCOBACTÉRIUM TURBERCULOSIS MULTIRÉSISTANTES AUX ANTIBIOTIQUES EN RÉPUBLIQUE CENTRAFRICAINE

[PDF] from univ-toulouse.fr

LX Nouvel - 2005 - oatao.univ-toulouse.fr... 61 FIGURE 14 : CARTE DES 41 LOCI MIRU SUR LE CHROMOSOME DE M.TUBERCULOSIS H37RV ..... 63 FIGURE 15 : DENDROGRAMME DES 79 PROFILSDE SPOLIGOTYPES DIFFERENTS DES 284 SOUCHES TYPEES. ...

[CITATION] CASE-REPORT: LUNGENTUBERKULOSE EINER MTA–NACHWEIS VON 3 NEUINFEKTIONEN DURCHSPOLIGOTYPING

B Werner, C Ellermeier, L Naumann… - Pneumologie, 2005

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[PDF] EDITORIAL BOARD• EDITORIAL BOARD

[PDF] from pasteur.ac.ir

T Bhatnagar, S Boutaiba… - FEMS …, 2005 - journals2005.pasteur.ac.irFEMS MICROBIOLOGY LETTERS Volume 248, Issue 2, Pages 133-274 (15 July 2005) 1. Editorialboard• EDITORIAL BOARD Pages iii-vi Research Letters 2. Lipolytic activity from Halobacteria:Screening and hydrolase production• SHORT COMMUNICATION Pages 133-140 ...Related articles - All 2 versions - Import into BibTeX

 

2004

 

PCR-based genotyping of Mycobacterium tuberculosis with new GC-rich repeated sequences and IS6110 inverted repeats used as primers

[HTML] from nih.gov

R Kotłowski, IC Shamputa, NA El Aila… - Journal of clinical …, 2004 - Am Soc Microbiol... In experimental studies, 27 M. tuberculosis isolates were analyzed. Ten colonies isolated from one sputum sample were subjected to spoligotyping (Isogen; Bioscience, Maarssen, The Netherlands) and IS6110-restriction fragment length polymorphism (RFLP) assay (13). ...

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[HTML] PCR-based genotyping of Mycobacterium tuberculosis with new GC-rich repeated sequences and IS6110 inverted repeats used as primers

[HTML] from ukpmc.ac.uk

R Kotlowski, IC Shamputa, NA El Aila… - JOURNAL OF …, 2004 - ukpmc.ac.uk... In experimental studies, 27 M. tuberculosis isolates were analyzed. Ten colonies isolated from one sputum sample were subjected to spoligotyping (Isogen; Bioscience, Maarssen, The Netherlands) and IS6110-restriction fragment length polymorphism (RFLP) assay (13). ...

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Molecular fingerprinting of Mycobacterium bovis subsp. caprae isolates from Central Europe

[HTML] from nih.gov

W Erler, G Martin, K Sachse… - Journal of clinical …, 2004 - Am Soc Microbiol... method (12) and subjected to direct-repeat-specific PCR and hybridization with membrane-boundspacer oligonucleotides and a spoligotyping kit (ISOGEN Bioscience, Maarsen, The ... Spoligotypingof the 79 isolates revealed 11 different spoligotypes (designated C1 to ...

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Analysis of clonal composition of Mycobacterium tuberculosis isolates in primary infections in children

[HTML] from nih.gov

DG de Viedma, M Marín, MJ Ruiz… - Journal of clinical …, 2004 - Am Soc Microbiol... The PCR for amplification of the DR region was performed with the primers in thespoligotyping kit (Isogen, Maarsen, The Netherlands) and according to the instructions ofthe manufacturer. All spoligotypes were confirmed by repeating the assay. ...

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Spoligotyping profile change caused by deletion of a direct variable repeat in a Mycobacterium tuberculosis isogenic laboratory strain

[HTML] from nih.gov

A Aranaz, B Romero, N Montero… - Journal of clinical …, 2004 - Am Soc Microbiol... The test was carried out with membranes obtained from Isogen Bioscience BV, Maarssen, The ...associated with the DR locus and their influence on the interpretation of spoligotyping. ...corresponding to relapses or infections at different sites showed identical spoligotypes (6, 10, ...

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Bovine tuberculosis (Mycobacterium bovis) in wildlife in Spain

[HTML] from nih.gov

A Aranaz, L De Juan, N Montero… - Journal of Clinical …, 2004 - Am Soc Microbiol... of the biotin-labeled PCR product onto a spoligotyping membrane (Isogen Bioscience BV ...Schematic representation of spoligotyping patterns identified among wildlife and areas where the patterns ... Six spoligotypes were identified among the isolates from Doñana National Park. ...

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[PDF] Simultaneous detection and typing of Mycobacterium tuberculosis complex bacteria

[PDF] from cli-online.com

M de Boer, A van der Zanden… - cli-online.com... of the mem- brane or by specific imaging systems developed by Isogen Life Science ... in thelaboratory, because different M. tuberculosis strains or DNA can yield different spoligotypes. Thusspoligotyping can be used for quality control of microscopy of AFB, cultures or nucleic ...

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Transmission of tuberculosis from smear negative patients: a molecular epidemiology study

[HTML] from highwire.org

E Hernandez-Garduno, V Cook, D Kunimoto… - Thorax, 2004 - thorax.highwire.org... A spoligotyping kit (Isogen Bioscience, Maarssen) was used as a secondary typingtechnique for strains with five copies or less of IS6110. 13 Smear specimens werestained with Auramine O and confirmed by Ziehl Neelsen staining. ...

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Characterization of ancestral Mycobacterium tuberculosis by multiple genetic markers and proposal of genotyping strategy

[HTML] from nih.gov

YJ Sun, ASG Lee, ST Ng, S Ravindran… - Journal of clinical …, 2004 - Am Soc Microbiol... Spoligotyping.Spoligotyping was performed using a commercial kit (Isogen Bioscience BV,Maarssen, The Netherlands) according to the instructions of the manufacturer. The spoligotypeswere presented by using pseudo-original patterns or 15 octal codes, as described by ...

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Genotypic and phenotypic heterogeneity among Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients

[HTML] from nih.gov

IC Shamputa, L Rigouts, LA Eyongeta… - Journal of clinical …, 2004 - Am Soc Microbiol... Spoligotyping.Spoligotyping was performed with a commercial kit (Isogen Bioscience BV,Maarssen, The ... All 10 clones from each of the 97 patients were tested by spoligotyping. ... of the 97patients tested, including the 7 Beijing genotype strains, showed identical spoligotypes. ...

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Relating the size of molecularly defined clusters of tuberculosis to the duration of symptoms

[HTML] from oxfordjournals.org

TP Giordano, H Soini, LD Teeter… - Clinical infectious …, 2004 - cid.oxfordjournals.org... BioImage). Isolates containing <5 IS6110 elements were spoligotyped [27] with acommercially available kit (Isogen Bioscience BV). Spoligotyping is an alternativemethod for molecular characterization of M. tuberculosis isolates. ...

Molecular epidemiology of drug-resistant Mycobacterium tuberculosis strains isolated from patients with pulmonary tuberculosis in Poland: a 1-year study

A Sajduda, A Brzostek, M Poplawska… - … of Tuberculosis and …, 2004 - ingentaconnect.com

... Spoligotyping was per- formed using a commercially available kit (Isogen Bioscience BV, Maarssen,The Netherlands) accord- ing to the ... In accordance with previous findings, spoligotyping was lessdiscrimina- tory than IS6110 RFLP.8,9 A total of 91 spoligotypes ...

Transmission characteristics of tuberculosis in the foreign-born and the Canadian-born populations of Alberta, Canada

D Kunimoto, K Sutherland… - … of Tuberculosis and …, 2004 - ingentaconnect.com... Two or more isolates with the same DNA fingerprint were called a cluster. A spoligotyping kit (Isogen Bioscience, Maarssen, The Netherlands) was used for secondary analysis of all strains with five copies or less of IS6110.6 ...

 

Species differentiation and antibiotic susceptibility testing with DNA microarrays

[PDF] from aseanbiotechnology.info

G Vernet, C Jay, M Rodrigue… - Journal of applied …, 2004 - Wiley Online Library... (Please refer to Fig. 3a.). 4.2.1 Reference spoligotyping. (Please refer to Fig. ... (1997). Reagents were provided by Isogen Life Science (The Netherlands). ... 4.2.2 Comparison of spoligotypes obtained by dot-blot and by the microarray with a strain of M. bovis. (Please refer to Fig. ...

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High rates of clustering of strains causing tuberculosis in Harare, Zimbabwe: a molecular epidemiological study

[HTML] from nih.gov

PJ Easterbrook, A Gibson, S Murad… - Journal of clinical …, 2004 - Am Soc Microbiol... primer 5′-CCAAGAGGGGACGGAAAC-3′ and biotinylated primer 5′-GGTTTTGGGTCTGACGAC-3′). The PCR products were then hybridized to a Biodyne C membrane (Isogen Bioscience, Maarsen ... By using spoligotyping alone, 54 distinct spoligotypes were identified ...

Identification of MDR-TB Beijing/W and other Mycobacterium tuberculosis genotypes in Nairobi, Kenya

WA Githui, AM Jordaan, ES Juma… - … of Tuberculosis and …, 2004 - ingentaconnect.com... Spoligotyping and analysis The method is based on in vitro PCR amplification of the ... Membranes and primers were obtained from Isogen, Bioscience BV, Utrecht, The Netherlands. ... were scanned and added to an existing spoligotype data- base containing spoligotypes from all ...

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First isolation of< i> Mycobacterium microti</i>(Llama-type) from a dog

L Deforges, HJ Boulouis, JL Thibaud… - Veterinary …, 2004 - Elsevier

... Definitive subspecies identification was established by spoligotyping (for “Spacer Oligotyping ...Easthill, NY, USA) sensitised with 43 aminolink–oligonucleotides, according to the procedures recommended by the manufacturer of the oligonucleotides (Isogen Bioscience, Maarssen ...

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Impact of drug resistance on fitness of Mycobacterium tuberculosis strains of the W‐Beijing genotype

OS Toungoussova, DA Caugant… - FEMS Immunology …, 2004 - Wiley Online Library ... 20,21]. Spoligotyping was performed by using a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the instructions supplied by the manufacturer, as previously described [22]. M. tuberculosis ...

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6 Molecular Epidemiology of Mycobacterium bow's

RA SKUCE… - Tuberculosis, 2004 - books.google.com

... e Spoligotyping result of M. tuberculosis H37Rv and M. bovis field isolates (rows), A membrane ...Courtesy of Isogen Bioscience BV representative panels of M. tuberculosis complex bac- teria(van ... 2000) and relatively large databases of spoligotypes are being curated (Sola et al. ...

Genomic characterization of an endemic Mycobacterium tuberculosis strain: evolutionary and epidemiologic implications

[HTML] from nih.gov

D Nguyen, P Brassard, D Menzies… - Journal of clinical …, 2004 - Am Soc Microbiol... Spoligotyping (or spacer oligonucleotide typing) was done by using a PCR-based technique which detects the presence or absence of 43 unique sequence spacers in the direct repeat (DR) locus (Isogen Bioscience BV, Maarssen, The Netherlands) (20). ...

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[HTML] Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques

[HTML] from scielo.br

PM Parreiras, FCF Lobato, AP Alencar… - Memórias do Instituto …, 2004 - SciELO Brasil... 2002), applied on the membrane using a Miniblotter MN45 (Isogen, the Netherlands) and after 1 min incubation at RT, the membrane was ... This observation was confirmed by the generation of M. bovis genotypes after submitting the isolates to spoligotyping (data not shown). ...

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[PDF] Antimicrobial resistance among Campylobacter strains, United States, 1997–2001

[PDF] from cdc.gov

A Gupta, JM Nelson, TJ Barrett, RV Tauxe… - Emerg Infect …, 2004 - wwwnc.cdc.gov Page 130. We summarize antimicrobial resistance surveillance data in human andchicken isolates of Campylobacter. Isolates were from a sentinel county study from1989 through 1990 and from nine state health departments ...

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Multicenter evaluation of reverse line blot assay for detection of drug resistance in Mycobacterium tuberculosis clinical isolates

[PDF] from rivm.nl

I Mokrousov, NV Bhanu, PN Suffys… - Journal of …, 2004 - Elsevier... Furthermore, the proposed method requires the same simple equipment as that used forspoligotyping and permits simultaneous analysis of ... Biodyne ®C membrane (Pall GelmanLaboratory, Ann Arbor, MI, USA) using the MN45 miniblotter apparatus (Isogen Biosci., Maarssen ...

PCR-BASED GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS WITH NEW GC-RICH REPEATED SEQUENCES AND IS6110 INVERTED REPEATS USED AS PRIMERS

[HTML] from nih.gov

R Kotłowski, IC Shamputa, NA El Aila… - Journal of clinical …, 2004 - Am Soc Microbiol... In experimental studies, 27 M. tuberculosis isolates were analyzed. Ten colonies isolated from one sputum sample were subjected to spoligotyping (Isogen; Bioscience, Maarssen, The Netherlands) and IS6110-restriction fragment length polymorphism (RFLP) assay (13). ...

ANALYSIS OF CLONAL COMPOSITION OF MYCOBACTERIUM TUBERCULOSIS ISOLATES IN PRIMARY INFECTIONS IN CHILDREN

[HTML] from nih.gov

DG de Viedma, M Marín, MJ Ruiz… - Journal of clinical …, 2004 - Am Soc Microbiol... The PCR for amplification of the DR region was performed with the primers in thespoligotyping kit (Isogen, Maarsen, The Netherlands) and according to the instructions ofthe manufacturer. All spoligotypes were confirmed by repeating the assay. ...Cited by 20 - Related articles - BL Direct - All 11 versions - Import into BibTeX

[HTML] PCR-BASED GENOTYPING OF MYCOBACTERIUM TUBERCULOSIS WITH NEW GC-RICH REPEATED SEQUENCES AND IS6110 INVERTED REPEATS USED AS PRIMERS

[HTML] from ukpmc.ac.uk

R Kotlowski, IC Shamputa, NA El Aila… - JOURNAL OF …, 2004 - ukpmc.ac.uk ... In experimental studies, 27 M. tuberculosis isolates were analyzed. Ten colonies isolated fro one sputum sample were subjected to spoligotyping (Isogen; Bioscience, Maarssen, The Netherlands) and IS6110-restriction fragment length polymorphism (RFLP) assay (13). ...

SPOLIGOTYPING PROFILE CHANGE CAUSED BY DELETION OF A DIRECT VARIABLE REPEAT IN A MYCOBACTERIUM TUBERCULOSIS ISOGENIC LABORATORY STRAIN

[HTML] from nih.gov

A Aranaz, B Romero, N Montero… - Journal of clinical …, 2004 - Am Soc Microbiol... The test was carried out with membranes obtained from Isogen Bioscience BV, Maarssen, The ...associated with the DR locus and their influence on the interpretation of spoligotyping. ...corresponding to relapses or infections at different sites showed identical spoligotypes (6, 10, ...

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BOVINE TUBERCULOSIS (MYCOBACTERIUM BOVIS) IN WILDLIFE IN SPAIN

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A Aranaz, L De Juan, N Montero… - Journal of Clinical …, 2004 - Am Soc Microbiol... of the biotin-labeled PCR product onto a spoligotyping membrane (Isogen Bioscience BV ...Schematic representation of spoligotyping patterns identified among wildlife and areas wherethe patterns ... Six spoligotypes were identified among the isolates from Doñana National Park. ...

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TRANSMISSION OF TUBERCULOSIS FROM SMEAR NEGATIVE PATIENTS: A MOLECULAR EPIDEMIOLOGY STUDY

[HTML] from highwire.org

E Hernandez-Garduno, V Cook, D Kunimoto… - Thorax, 2004 - thorax.highwire.org... A spoligotyping kit (Isogen Bioscience, Maarssen) was used as a secondary typingtechnique for strains with five copies or less of IS6110. 13 Smear specimens were stained with Auramine O and confirmed by Ziehl Neelsen staining. ...

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CHARACTERIZATION OF ANCESTRAL MYCOBACTERIUM TUBERCULOSIS BY MULTIPLE GENETIC MARKERS AND PROPOSAL OF GENOTYPING STRATEGY

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YJ Sun, ASG Lee, ST Ng, S Ravindran… - Journal of clinical …, 2004 - Am Soc Microbiol... Spoligotyping.Spoligotyping was performed using a commercial kit (Isogen Bioscience BV,Maarssen, The Netherlands) according to the instructions of the manufacturer. The spoligotypes were presented by using pseudo-original patterns or 15 octal codes, as described by ...

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GENOTYPIC AND PHENOTYPIC HETEROGENEITY AMONG MYCOBACTERIUM TUBERCULOSIS ISOLATES FROM PULMONARY TUBERCULOSIS PATIENTS

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IC Shamputa, L Rigouts, LA Eyongeta… - Journal of clinical …, 2004 - Am Soc Microbiol... Spoligotyping.Spoligotyping was performed with a commercial kit (Isogen Bioscience BV,Maarssen, The ... All 10 clones from each of the 97 patients were tested by spoligotyping. ... of the 97patients tested, including the 7 Beijing genotype strains, showed identical spoligotypes. ...

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HIGH RATES OF CLUSTERING OF STRAINS CAUSING TUBERCULOSIS IN HARARE, ZIMBABWE: A MOLECULAR EPIDEMIOLOGICAL STUDY

[HTML] from nih.gov

PJ Easterbrook, A Gibson, S Murad… - Journal of clinical …, 2004 - Am Soc Microbiol... primer 5′-CCAAGAGGGGACGGAAAC-3′ and biotinylated primer 5′-GGTTTTGGGTCTGACGAC-3′). The PCR products were then hybridized to a Biodyne C membrane (IsogenBioscience, Maarsen ... By using spoligotyping alone, 54 distinct spoligotypes were identified ...Cited by 37 - Related articles - BL Direct - All 12 versions - Import into BibTeX

 

2003

 

Evaluation of the epidemiologic utility of secondary typing methods for differentiation of Mycobacterium tuberculosis isolates

[HTML] from nih.gov

A Kwara, R Schiro, LS Cowan… - Journal of clinical …, 2003 - Am Soc Microbiol

... Spoligotyping (Isogen Bioscience BV, Maarssen, The Netherlands) was performed in accordance with previously published methods (8). Results were recorded as an octal code (4). MIRU loci (10) were amplified with primers specific for sequences flanking each locus (Table 1 ...

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Characterization of Mycobacterium tuberculosis complex DNAs from Egyptian mummies byspoligotyping

[HTML] from nih.gov

AR Zink, C Sola, U Reischl, W Grabner… - Journal of clinical …, 2003 - Am Soc Microbiol

... For this analysis, we used a commercial spoligotyping kit (Isogen, Maarsen, The Netherlands ...it has been shown that the absence of spacers 33 to 36 in the spoligotyping signatures of ... absence of spacers 33 to 36 (Table 4), most of the M. tuberculosis spoligotypes obtained from ...

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Characterization of Finnish Mycobacterium tuberculosis isolates by spoligotyping

[HTML] from nih.gov

K Puustinen, M Marjamäki, N Rastogi… - Journal of clinical …, 2003 - Am Soc Microbiol... Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,The Netherlands) according to the instructions of the manufacturer. ... We analyzed 380 M. tuberculosis isolates by spoligotyping and obtained a total of 138 spoligotypes. ...

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Widespread pyrazinamide-resistant Mycobacterium tuberculosis family in a low-incidence setting

[HTML] from nih.gov

D Nguyen, P Brassard, J Westley… - Journal of clinical …, 2003 - Am Soc Microbiol... IS6110 RFLP by Southern blotting (38) and spoligotyping (Isogen Bioscience BV) (26) were performed using ... the observation that the DNA fingerprints by both IS6110 RFLP and spoligotyping are similar ... approximately 2 to 3.2 years (13, 42) and that of spoligotypes is believed to ...

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Molecular epidemiology and drug resistance of Mycobacterium tuberculosis isolates in the Archangel prison in Russia: predominance of the W-Beijing clone family

[HTML] from oxfordjournals.org

OS Toungoussova, A Mariandyshev… - Clinical infectious …, 2003 - cid.oxfordjournals.org... Spoligotyping was performed using a commercially available spoligotyping kit (Isogen BioscienceBV) in ... were represented by 6 and 3 isolates each, and 4 spoligotypes comprised 2 ... All isolateswith identical IS6110 RFLP patterns were found to be identical by spoligotyping. ...Cited by 62 - Related articles - BL Direct - All 12 versions - Import into BibTeX

Molecular typing of Mycobacterium tuberculosis by using nine novel variable-number tandem repeats across the Beijing family and low-copy-number IS6110 isolates

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RS Spurgiesz, TN Quitugua, KL Smith… - Journal of clinical …, 2003 - Am Soc Microbiol... In a database of 3,319 spoligotypes from isolates collected in geographically distinct areas, seven spoligotype ... Advanced Nucleic Acids Technology Core Facility, San Antonio, Tex.) and commercially available spoligotyping membranes (Isogen, Maarssen, The ...

The molecular epidemiology of tuberculosis in western CanadaJM FitzGerald, A Fanning… - … of Tuberculosis and …, 2003 - ingentaconnect.com... All isolates matched as identical by computer were manually confirmed by visually comparingthe original autoradiographs. A spoligotyping kit (Isogen Bioscience, Maarssen, The Netherlands)was used for secondary analysis of 90 strains with five copies or less of IS6110. ...

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Genetic biodiversity of Mycobacterium tuberculosis complex strains from patients with pulmonary tuberculosis in Cameroon

[HTML] from nih.gov

SN Niobe-Eyangoh, C Kuaban, P Sorlin… - Journal of clinical …, 2003 - Am Soc Microbiol

... (i) Spoligotyping.Spoligotyping was performed ... as a probe to hybridize with 43 synthetic spacer oligonucleotides covalently bound to a membrane (Isogen Biosciences BV ... Spoligotypes were analyzed with the Bionumerics program version 2.5 (Applied Maths, Kortrijk, Belgium). ...

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Detection of pathogenic mycobacteria of veterinary importance

RA Skuce, MS Hughes, MJ Taylor… - METHODS IN MOLECULAR …, 2003 - Springer... 2.5.3. Membrane-Based Analysis 1. Spoligotyping Kit (Isogen Bioscience BV, Maarssen,Netherlands). 2. SSPE buffer (2X): 1X SSPE is 0.18 M HCl, 10 mM NaH2PO4, 1 mM EDTA.To be supplemented with 0.1 % sodium dodecyl sulfate (SDS). ...

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Analysis of multidrug-resistant Mycobacterium bovis from three clinical samples from Scotland

VM Hughes, R Skuce, C Doig… - … of Tuberculosis and …, 2003 - ingentaconnect.com... from each isolate was subjected to spoligotyping,11 exactly as specified by the kit manufacturers(Isogen Bioscience BV ... linked as they shared spoligotyping and DRr-RFLP profiles. The DRr-RFLPprofiles and spoligotypes shared by Cases I/94 and II/98 have never previously ...Cited by 8 - Related articles - BL Direct - All 5 versions - Import into BibTeX

[PDF] ÇOĞUL DİRENÇLİ MYCOBACTERİUM TUBERCULOSİS İZOLATLARINDA SEKONDER İLAÇLARA DİRENÇ VE

[PDF] from ulakbim.gov.tr

E TAKİP - 2003 - uvt.ulakbim.gov.tr... Spoligotyping için ticari kit kullanılmıştır (Isogen Bioscience BV, Maarssen, The Netherlands ... inCuba outside of Havana, July 1994- June 1995: utility of spoligotyping versus IS6110 ... BN, KremerK., Valetudie G. et al, Global distribution of Mycobacterium tuberculosis spoligotypes. ...

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Investigations into an outbreak of tuberculosis in a flock of sheep in contact with tuberculous cattle

FE Malone, EC Wilson, JM Pollock… - Journal of Veterinary …, 2003 - Wiley Online Library... Molecular strain typing was undertaken on 39 cattle and three sheep M. bovis isolates usingspoligotyping (Kamerbeek et al., 1997) exactly as directed by the manufacturer (Isogen Bioscience,Netherlands) and a selection of six VNTR markers (Skuce and Neill, 2001). ...

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IS6110-mediated deletion polymorphism in the direct repeat region of clinical isolates of Mycobacterium tuberculosis

[HTML] from nih.gov

SL Sampson, RM Warren, M Richardson… - Journal of …, 2003 - Am Soc Microbiol... These isolates were chosen on the basis of spoligotypes, which indicated a deletion of ...Spoligotyping.DNA polymorphism in the DR locus was detected by spoligotyping according toa ... products were then hybridized to a commercially available membrane (Isogen Biosciences ...

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Heterogeneity of Mycobacterium tuberculosis isolates in Yangon, Myanmar

S Phyu, R Jureen, T Ti, UR Dahle… - Journal of clinical …, 2003 - Am Soc Microbiol... susceptibility test, niacin accumulation test, nitrate reduction test, and pyrazinamidase activity)were performed for phenotyping (4). Spoligotyping was performed with a commercial kit asrecommended by the manufacturer (Isogen Bioscience BV, Maarssen, The Netherlands). ...

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Single nucleotide polymorphisms in genes associated with isoniazid resistance in Mycobacterium tuberculosis

[HTML] from nih.gov

SV Ramaswamy, R Reich, SJ Dou… - Antimicrobial agents …, 2003 - Am Soc Microbiol

... Spoligotyping and major genetic group assignment.Low-copy-number isolates were subjected to a secondary typing method by use of spoligotyping. This method was performed with a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to ...

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Apport épidémiologique du typage moléculaire des bacilles de la tuberculose

V Vincent… - Médecine et Maladies Infectieuses, 2003 - Elsevier... à partir d'un lysat cellulaire utilisé comme source d'ADN, puis hybridée à une membrane sur laquelle sont fixés les 43 séquences inter DR les plus fréquentes (la membrane est commercialisée par Isogen Bioscience BV, Maarsen, Hollande) (Fig. 2). Le spoligotyping est ...

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[CITATION] Multicenter Evaluation of Reverse Line Blot Assay to Predict Drug Resistance in Mycobacterium tuberculosis Clinical IsolatesI Mokrousov, NV Bhanu, PN Suffys, GV Kadival…

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EVALUATION OF THE EPIDEMIOLOGIC UTILITY OF SECONDARY TYPING METHODS FOR DIFFERENTIATION OF MYCOBACTERIUM TUBERCULOSIS ISOLATES

[HTML] from nih.gov

A Kwara, R Schiro, LS Cowan… - Journal of clinical …, 2003 - Am Soc Microbiol

... Spoligotyping (Isogen Bioscience BV, Maarssen, The Netherlands) was performed in accordance with previously published methods (8). Results were recorded as an octal code (4). MIRU loci (10) were amplified with primers specific for sequences flanking each locus (Table 1 ...

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CHARACTERIZATION OF FINNISH MYCOBACTERIUM TUBERCULOSIS ISOLATES BY SPOLIGOTYPING

[HTML] from nih.gov

K Puustinen, M Marjamäki, N Rastogi… - Journal of clinical …, 2003 - Am Soc Microbiol... Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,The Netherlands) according to the instructions of the manufacturer. ... We analyzed 380 M.tuberculosis isolates by spoligotyping and obtained a total of 138 spoligotypes. ...

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WIDESPREAD PYRAZINAMIDE-RESISTANT MYCOBACTERIUM TUBERCULOSIS FAMILY IN A LOW-INCIDENCE SETTING

[HTML] from nih.gov

D Nguyen, P Brassard, J Westley… - Journal of clinical …, 2003 - Am Soc Microbiol... IS6110 RFLP by Southern blotting (38) and spoligotyping (Isogen Bioscience BV) (26) wereperformed using ... the observation that the DNA fingerprints by both IS6110 RFLP and spoligotypingare similar ... approximately 2 to 3.2 years (13, 42) and that of spoligotypes is believed to ...

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CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX DNAS FROM EGYPTIAN MUMMIES BYSPOLIGOTYPING

[HTML] from nih.gov

AR Zink, C Sola, U Reischl, W Grabner… - Journal of clinical …, 2003 - Am Soc Microbiol... For this analysis, we used a commercial spoligotyping kit (Isogen, Maarsen, The Netherlands ...it has been shown that the absence of spacers 33 to 36 in the spoligotyping signatures of ... absenceof spacers 33 to 36 (Table 4), most of the M. tuberculosis spoligotypes obtained from ...

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2002

 

Tuberculosis recurrences: reinfection plays a role in a population whose clinical/epidemiological characteristics do not favor reinfection

[HTML] from ama-assn.org

DG de Viedma, M Marin, S Hernangomez… - Archives of internal …, 2002 - Am Med Assoc... The polymerase chain reaction (PCR) for amplifying the directed repeats region was performed using primers of the spoligotyping kit (Isogen Bioscience, Maarssen, the Netherlands), following the manufacturer's instructions. All spoligotypes showing differences for the strains ...

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[HTML] Molecular differentiation of Mycobacterium tuberculosis strains without IS6110 insertions

[HTML] from nih.gov

KH Lok, WH Benjamin Jr, ME Kimerling… - Emerging infectious …, 2002 - ncbi.nlm.nih.gov... Spoligotyping membranes were purchased from Isogen Bioscience BV (Isogen, Bilthoven, theNetherlands). ... repeats results of three zero-copy IS6110 isolates with identical spoligotyping andpolymorphic ... Fourteen of 32 spoligotyped strains are similar and have the same CDC ...

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Variable-number tandem repeat typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110 by using mycobacterial interspersed repetitive units

[HTML] from nih.gov

LS Cowan, L Mosher, L Diem… - Journal of clinical …, 2002 - Am Soc Microbiol... Spoligotyping.Spoligotyping was performed using a commercial kit (Isogen Bioscience BV,Maarssen, The ... Q could also be divided by IS6110 RFLP and spoligotyping into subclusters characterized by different insertion sites and divergent spoligotypes, as described ...

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Infection of red deer, cattle, and humans with Mycobacterium bovis subsp. caprae in western Austria

[HTML] from nih.gov

WM Prodinger, A Eigentler… - Journal of clinical …, 2002 - Am Soc Microbiol... on membranes with covalently bound, spacer sequence-specific oligonucleotides purchased from Isogen Bioscience BV ... region I isolates (3 cattle, 1 deer); lanes 5 to 9, spoligotypes from four ...wax-embedded tissue from that animal yielded the region II spoligotyping pattern (Fig. ...

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Transmission of drug-resistant tuberculosis in Texas and Mexico

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TN Quitugua, BJ Seaworth, SE Weis… - Journal of clinical …, 2002 - Am Soc Microbiol... Locally synthesized and biotinylated PCR primers (Advanced Nucleic Acids TechnologyCore Facility, UTHSCSA) were used with commercially available spoligotyping membranes (Isogen, Maarssen, The Netherlands). Spoligotyping ...

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Spread of drug-resistant Mycobacterium tuberculosis strains of the Beijing genotype in the Archangel Oblast, Russia

[HTML] from nih.gov

OS Toungoussova, P Sandven… - Journal of clinical …, 2002 - Am Soc Microbiol... Spoligotyping was performed by using a commercially available kit (Isogen Bioscience BV ...Spoligotyping.The spoligotyping method was used as an additional tool to further ... to the Beijing genotype by their IS6110 RFLP patterns showed identical spoligotypes, lacking spacers 1 ...

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[HTML] Molecular typing of Mycobacterium tuberculosis strains with a common two-band IS6110 pattern

[HTML] from nih.gov

KH Lok, WH Benjamin Jr, ME Kimerling… - Emerging infectious …, 2002 - ncbi.nlm.nih.gov... Spoligotyping membranes were used according to manufacturer's recommendations (Isogen Bioscience BV, Bilthoven ... well between these sizes, which could result in different spoligotypes of a ... Using VNTR and spoligotyping techniques to reexamine some of these clusters, we ...

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Sputum induction problems identified through genetic fingerprinting

B Nivin, T O'Flaherty, E Leibert, BY Zhao… - Infection control and …, 2002 - JSTOR... Because spoligotyping is less discriminatory than IS6110-based analysis, identical spoligotypesmay indi- cate the need for further confirmation using the latter method. ... The spoligotyping analysiswas performed using a commercially available kit (Isogen Bioscience BV ...

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Isoniazid resistance, mycobacterial genotype and outcome in Vietnamese adults with tuberculous meningitis

GE Thwaites, TTH Chau, M Caws… - … of Tuberculosis and …, 2002 - ingentaconnect.com... was car- ried out in accordance with a standardised protocol for spoligotyping.16 Briefly ... were heat-denatured and hybridised to the spoligotype membrane (Isogen Biosciences, Asmaars ...Patterns were compared against the Beijing and 'Vietnam' spoligotypes described by Anh et ...

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Biochemical and genetic evidence for the transfer of Mycobacterium tuberculosis subsp. caprae Aranaz et al. 1999 to the species Mycobacterium bovis Karlson and …

[PDF] from sgmjournals.org

S Niemann, E Richter… - International journal of …, 2002 - Soc General Microbiol... Spoligotyping, which is based on the detection of various non- repetitive spacer sequences located between small repetitive units (direct ... blotting technique, was performed as described previously (Kamerbeek et al., 1997) by using a commercially available membrane (Isogen). ...

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Molecular epidemiology and drug resistance of Mycobacterium tuberculosis isolates from Ethiopian pulmonary tuberculosis patients with and without human …

[HTML] from nih.gov

J Bruchfeld, G Aderaye, IB Palme… - Journal of clinical …, 2002 - Am Soc Microbiol... synthetic spacer oligonucleotides which are covalently bound to a membrane (Isogen Bioscience BV ... 24, 41); and they all lacked the typical M. canetti spoligotyping patterns (presence of ... 3. Schematic representation of the spoligotypes of M. tuberculosis isolates belonging to four ...

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[HTML] Genotyping analyses of tuberculosis cases in US-and foreign-born Massachusetts residents

[HTML] from nih.gov

S Sharnprapai, AC Miller, R Suruki… - Emerging infectious …, 2002 - ncbi.nlm.nih.gov... with the molecular weight standards provided by CDC. Spoligotyping was performed with a commercially available kit, in accordance with the manufacturer's instructions(Isogen Bioscience BV, Maarseen, the Netherlands). ...

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[HTML] Statewide Molecular Epidemiology of Mycobacterium tuberculosis Transmission in a Moderate-to Low-Incidence State: Are Contact Investigations Enough?

[HTML] from nih.gov

WA Cronin, JE Golub, MJ Lathan… - Emerging Infectious …, 2002 - ncbi.nlm.nih.gov... Spoligotyping was performed for all matching strains that had six or more IS6110copies by using a commercially available kit, according to the manufacturer'sinstructions (Isogen Bioscience BV, Maarssen, the Netherlands) (13). ...

Tuberculosis

[PDF] from ascpjournals.org

Q Eichbaum… - American Journal of Clinical …, 2002 - ajcp.ascpjournals.org... Quentin Eichbaum, MD, PhD, MPH,1 and Eric J. Rubin, MD, PhD2 Key Words: Susceptibility;Rapid culture; Molecular beacons; Nested PCR; Ligation; Spoligotyping; Strains DOI:

10.1309/XX1NBANX8JKMTUQN Page 2. Eichbaum and Rubin / TUBERCULOSIS ...

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[HTML] STATEWIDE MOLECULAR EPIDEMIOLOGY OF MYCOBACTERIUM TUBERCULOSIS TRANSMISSION IN A MODERATE-TO LOW-INCIDENCE STATE: ARE CONTACT INVESTIGATIONS ENOUGH?

[HTML] from nih.gov

WA Cronin, JE Golub, MJ Lathan… - Emerging Infectious …, 2002 - ncbi.nlm.nih.gov... Spoligotyping was performed for all matching strains that had six or more IS6110copies by using a commercially available kit, according to the manufacturer'sinstructions (Isogen Bioscience BV, Maarssen, the Netherlands) (13). ...

BIOCHEMICAL AND GENETIC EVIDENCE FOR THE TRANSFER OF MYCOBACTERIUM TUBERCULOSIS SUBSP. CAPRAE ARANAZ ET AL. 1999 TO THE SPECIES MYCOBACTERIUM BOVIS KARLSON AND …

[PDF] from sgmjournals.org

S Niemann, E Richter… - International journal of …, 2002 - Soc General Microbiol... Spoligotyping, which is based on the detection of various non- repetitive spacer sequences locatedbetween small repetitive units (direct ... blotting technique, was performed as described previously(Kamerbeek et al., 1997) by using a commercially available membrane (Isogen). ...

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2001

 

Transmission of Mycobacterium tuberculosis to a Funeral Director During Routine Embalming*

[HTML] from chestpubs.org

M Lauzardo, P Lee, H Duncan… - Chest, 2001 - chestjournal.chestpubs.org... Secondary strain typing of M tuberculosis was performed by spoligotyping (IsogenBioscience, BV; Maarssen, The Netherlands) as described by Kamerbeek et al. 10Spacer regions within the direct repeat locus of the chromosome ...Cited by 9 - Related articles - BL Direct - All 5 versions - Import into BibTeX

Epidemiologic Usefulness of Spoligotyping for Secondary Typing ofMycobacterium tuberculosis Isolates with Low Copy Numbers of IS6110

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WA Cronin, JE Golub, LS Magder… - Journal of clinical …, 2001 - Am Soc Microbiol... Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered strains ... investigation of low-copy clusters to those with rare spoligotypes may also ... In conclusion, spoligotyping did not appreciably improve strain differentiation among isolates with ...

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Mycobacterium tuberculosis complex DNA from an extinct bison dated 17,000 years before the present

[HTML] from oxfordjournals.org

BM Rothschild, LD Martin, G Lev… - Clinical Infectious …, 2001 - cid.oxfordjournals.org... The sequence was analyzed in an ABI PRISM 310 automated sequencer (PE Applied Biosystems).Spoligotyping. To determine the species of Mycobacterium within the M. tuberculosis complex,the spoligotyping system (Isogen) was applied in the Jerusalem laboratory. ...

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Spread of drug-resistant pulmonary tuberculosis in Estonia

[HTML] from nih.gov

A Krüüner, SE Hoffner, H Sillastu… - Journal of clinical …, 2001 - Am Soc Microbiol... Membranes for spoligotyping were obtained from Isogen, Bioscience BV, Utrecht, The Netherlands. ...Genetic homogenity of family A confirmed by spoligotyping.The 12 strains from family A showedan ... The spoligotypes of the non-Beijing family strains were highly diverse (Table 2 ...

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Transmission dynamics and molecular characterization of Mycobacterium tuberculosis isolates with low copy numbers of IS6110

[HTML] from nih.gov

H Soini, X Pan, L Teeter, JM Musser… - Journal of clinical …, 2001 - Am Soc Microbiol... Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV,Maarssen, The Netherlands) according to the instructions of the manufacturer. ...Spoligotyping.A total of 72 spoligotypes was identified among the 377 isolates

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Identification of a contaminating Mycobacterium tuberculosis strain with a transposition of an IS6110insertion element resulting in an altered spoligotype

[HTML] from nih.gov

WH Benjamin Jr, KH Lok, R Harris… - Journal of clinical …, 2001 - Am Soc Microbiol... 36-bp direct repeats of M. tuberculosis, was done using membranes from Isogen BiosciencesBV ... of direct repeat regions and thus loss of spacers on spoligotyping have been ... The second report explains differences between two common spoligotypes found in the IS6110 Harlem ...

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Spoligotype diversity of Mycobacterium bovis strains isolated in France from 1979 to 2000

[HTML] from nih.gov

N Haddad, A Ostyn, C Karoui… - Journal of clinical …, 2001 - Am Soc Microbiol... “None” corresponds to departments where two or more spoligotypes were codominant ... (6), was used for the spoligotyping of M ... of 43 aminolink oligonucleotides by following the procedures recommended by the manufacturer of the oligonucleotides (Isogen Bioscience, Maarssen ...

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Risk of Mycobacterium tuberculosis transmission in a low-incidence country due to immigration from high-incidence areas

[HTML] from nih.gov

T Lillebaek, ÅB Andersen, J Bauer… - Journal of clinical …, 2001 - Am Soc Microbiol... For spoligotyping, DNA extraction was performed by mechanical disruption of the cells with glass beads by vortexing either a few colonies from solid media or 100 μl of the frozen culture stock(27). The membranes used (Isogen; Bioscience BV, Utrecht, The Netherlands ...

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TRANSMISSION OF MYCOBACTERIUM TUBERCULOSIS TO A FUNERAL DIRECTOR DURING ROUTINE EMBALMING*

[HTML] from chestpubs.org

M Lauzardo, P Lee, H Duncan… - Chest, 2001 - chestjournal.chestpubs.org... Secondary strain typing of M tuberculosis was performed by spoligotyping (Isogen Bioscience, BV; Maarssen, The Netherlands) as described by Kamerbeek et al. 10 Spacer regions within the direct repeat locus of the chromosome ...

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EPIDEMIOLOGIC USEFULNESS OF SPOLIGOTYPING FOR SECONDARY TYPING OFMYCOBACTERIUM TUBERCULOSIS ISOLATES WITH LOW COPY NUMBERS OF IS6110

[HTML] from nih.gov

WA Cronin, JE Golub, LS Magder… - Journal of clinical …, 2001 - Am Soc Microbiol ... Maryland culture-positive tuberculosis (TB) patients with low-copy IS6110-spoligotyped clustered strains ... investigation of low-copy clusters to those with rare spoligotypes may also ... In conclusion, spoligotyping did not appreciably improve strain differentiation among isolates with ...

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MYCOBACTERIUM TUBERCULOSIS COMPLEX DNA FROM AN EXTINCT BISON DATED 17,000 YEARS BEFORE THE PRESENT

[HTML] from oxfordjournals.org

BM Rothschild, LD Martin, G Lev… - Clinical Infectious …, 2001 - cid.oxfordjournals.org... The sequence was analyzed in an ABI PRISM 310 automated sequencer (PE Applied Biosystems). Spoligotyping. To determine the species of Mycobacterium within the M. tuberculosis complex, the spoligotyping system (Isogen) was applied in the Jerusalem laboratory. ...

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2000

Characterization of Mycobacterium tuberculosis isolates from patients in Houston, Texas, byspoligotyping

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H Soini, X Pan, A Amin, EA Graviss… - Journal of clinical …, 2000 - Am Soc Microbiol... Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen, The ... square analysis was used to test the association of clustered spoligotypes with IS6110 ... The sensitivity and specificity of spoligotyping were calculated by a method described ...Cited by 110 - Related articles - BL Direct - All 9 versions - Import into BibTeX

Use of spoligotype analysis to detect laboratory cross-contamination

B Nivin, J Driscoll, T Glaser, P Bifani… - Infection control and …, 2000 - JSTOR... The spoligotyping analysis was performed using a commercially available kit (Isogen Bioscience BV ... In several cases of identical spoligotypes, the Wadsworth Center indicated that the ...Spoligotyping is an effective, relatively inexpensive (approximately 10% the cost of RFLP ...Cited by 12 - Related articles - BL Direct - All 6 versions - Import into BibTeX

Differentiation among Members of the Mycobacterium tuberculosis Complex by Molecular and Biochemical Features: Evidence for Two Pyrazinamide-Susceptible …

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S Niemann, E Richter… - Journal of clinical …, 2000 - Am Soc Microbiol... sequences which were bound on a nylon membrane by reverse line blotting (Isogen, Maarssen,The ... PZA, and source of isolates.All isolates have been analyzed by spoligotyping and were ...comprising at least two isolates could be found (Fig.1). The spoligotypes among isolates ...Cited by 87 - Related articles - BL Direct - All 11 versions - Import into BibTeX

Drug resistance rates of Mycobacterium tuberculosis strains in Austria between 1995 and 1998 and molecular typing of multidrug-resistant isolates

F Stauffer, A Makristathis, JP Klein… - Epidemiology and …, 2000 - Cambridge Univ Press... se- quences of M. tuberculosis H37Rv and M. bo is BCG P3, being immobilized on a membranedelivered by Isogen Bioscience (Maarssen ... 1. Spoligotyping of MDR MTB strains isolated be-tween 1995 and 1998 in Austria: Lanes 1 and 19 represent the spoligotypes of the ...Cited by 4 - Related articles - BL Direct - All 10 versions - Import into BibTeX

Principe et intéręt du typage moléculaire appliqué a Mycobacterium tuberculosis

V Vincent… - Revue Francaise des Laboratoires, 2000 - Elsevier... La methode 26 spoligotyping repose sur la detection du polymorphisme des ces sequences non conservees. ... douteux (figure .2). La realisation de la methode est facilitee par la commercialisation de membranes portent l'ensemble de ces 43 sequences (Isogen Bioscience ...

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1999

 

Usefulness of Spoligotyping To Discriminate IS6110Low-Copy-Number Mycobacterium tuberculosis Complex Strains Cultured in Denmark

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J Bauer, ÅB Andersen, K Kremer… - Journal of clinical …, 1999 - Am Soc Microbiol... culture stock) with glass beads by vortexing (26). Membranes for spoligotyping were obtained from Isogen, Bioscience BV, Utrecht, The Netherlands. The membranes contained oligonucleotides derived from the spacer DNA ...

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DNA typing of a nonviable culture of Mycobacterium tuberculosis in a homeless shelter outbreak

[HTML] from nih.gov

JR Driscoll, MA McGarry… - Journal of clinical …, 1999 - Am Soc Microbiol ... Spoligotyping was performed on all the 1997 isolates from individuals involved in the outbreak, and from a sample of nonviable cells from the 1992 culture of patient A, by using a commercially available spoligotyping kit (Isogen, Maarssen, The Netherlands). ...

Comparative Evaluation of Ligation-Mediated PCR and Spoligotyping as Screening Methods for Genotyping ofMycobacterium tuberculosis Strains

[HTML] from nih.gov

S Bonora, MC Gutierrez, G Di Perri… - Journal of clinical …, 1999 - Am Soc Microbiol

... (i) Spoligotyping.Spoligotyping was performed ... probe to hybridize with 43 synthetic spacer oligonucleotides attached to a carrier membrane (Isogen Bioscience BV ... The software-assisted evaluation of spoligotypes was done with GelCompar software, version 3.1b (Applied Maths ...

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Molecular fingerprinting confirms extensive cow-to-cow intra-herd transmission of a single Mycobacterium bovis strain

VS Perumaalla, LG Adams, J Payeur, D Baca… - Veterinary …, 1999 - Elsevier... Spoligotyping was performed according to protocol described by the manufacturer (IsogenBioscience BV, Maarssen, The Netherlands). The method is based on amplification of the

polymorphic DR locus that is specific to organisms in the M. tuberculosis complex. ...

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DNA typing of Mycobacterium bovis strains from the Castlepoint area of the Wairarapa

DM Collins - New Zealand Veterinary Journal, 1999 - Taylor & Francis... For spoligotyping, filters containing the appropriate array of primers (Isogen Bioscience, TheNetherlands ... 24 restriction types were differenti- ated into 11 PGRS types and 7 spoligotypes.Combining the results of both PGRS typing and spoligotyping, dif- ferentiated the isolates ...

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Extensive Cross-Contamination of Specimens withMycobacterium tuberculosis in a Reference Laboratory

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MC Ramos, H Soini, GC Roscanni… - Journal of clinical …, 1999 - Am Soc Microbiol... Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,The Netherlands ... obtained between 8 May and 18 July 1995 were characterized by spoligotypingand IS6110 ... Seventeen different spoligotypes were identified among the 91M. ...

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Transmission dynamics of tuberculosis in a high-incidence country: prospective analysis by PCR DNA fingerprinting

[HTML] from nih.gov

WH Haas, G Engelmann, B Amthor… - Journal of clinical …, 1999 - Am Soc Microbiol... Spoligotyping.Spoligotyping was performed as previously described by Kamerbeek et al. (10). ...A total of 20 μl of the amplification product was hybridized with 43 oligonucleotides covalently

bound to a membrane (Isogen Bioscience BV ... Analysis of spoligotypes was done by eye. ...

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Evolution and clonal traits of Mycobacterium tuberculosis complex in Guinea-Bissau

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G Källenius, T Koivula… - Journal of clinical …, 1999 - Am Soc Microbiol... synthetic spacer oligonucleotides which are covalently bound to a membrane (Isogen BioscienceBV ... of copies of IS6110 elements, and frequently lack the spoligotyping spacers 33 ... M.tuberculosisisolates in terms of biochemical traits, RFLP patterns, and spoligotypes when they ...

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Molecular Markers Demonstrate that the First Described Multidrug-Resistant Mycobacterium bovisOutbreak Was Due to Mycobacterium tuberculosis

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MC Gutierrez, JC Galan, J Blazquez… - Journal of clinical …, 1999 - Am Soc Microbiol... (v) Characterization of the DR region by spoligotyping.The DR cluster DNA of the strains wasamplified by PCR. The labeled PCR product was used as a probe to hybridize with 43 syntheticspacer oligonucleotides attached to a carrier membrane (Isogen Bioscience BV), as ...

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MOLECULAR FINGERPRINTING CONFIRMS EXTENSIVE COW-TO-COW INTRA-HERD TRANSMISSION OF A SINGLE MYCOBACTERIUM BOVIS STRAIN

VS Perumaalla, LG Adams, J Payeur, D Baca… - Veterinary …, 1999 - Elsevier

... Spoligotyping was performed according to protocol described by the manufacturer (IsogenBioscience BV, Maarssen, The Netherlands). The method is based on amplification of the polymorphic DR locus that is specific to organisms in the M. tuberculosis complex. ...

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DNA TYPING OF MYCOBACTERIUM BOVIS STRAINS FROM THE CASTLEPOINT AREA OF THE WAIRARAPA

DM Collins - New Zealand Veterinary Journal, 1999 - Taylor & Francis

... For spoligotyping, filters containing the appropriate array of primers (Isogen Bioscience, The Netherlands ... 24 restriction types were differenti- ated into 11 PGRS types and 7 spoligotypes. Combining the results of both PGRS typing and spoligotyping, differentiated the isolates ...

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MOLECULAR CLUES OF A MICROEPIDEMY AMONG HOMELESS TUBERCULOSIS PATIENTS IN BUDAPEST DUE TO A NEW AND LOCAL MYCOBACTERIUM TUBERCULOSIS CLADE

C Ködmön, S Niemann, MC Gutierrez… - Infection, Genetics and …, 2007 - Elsevier... Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands) according to the instructions of the manufacturer (Kremer et al., 1999). Briefly, genomic mycobacterial DNA ...

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TRANSMISSION DYNAMICS OF TUBERCULOSIS IN A HIGH-INCIDENCE COUNTRY: PROSPECTIVE ANALYSIS BY PCR DNA FINGERPRINTING

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WH Haas, G Engelmann, B Amthor… - Journal of clinical …, 1999 - Am Soc Microbiol... Spoligotyping.Spoligotyping was performed as previously described by Kamerbeek et al. (10). ...A total of 20 μl of the amplification product was hybridized with 43 oligonucleotides covalently bound to a membrane (Isogen Bioscience BV ... Analysis of spoligotypes was done by eye. ...

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MYCOBACTERIUM TUBERCULOSIS GENOTYPES AND THEIR RELATIONSHIPS WITH CLINICAL AND IMMUNOLOGICAL PHENOTYPES IN SINGAPORE

[PDF] from 137.132.14.55

SUN YONGJIANG - 2006 - 137.132.14.55

... Furthermore, this method is highly reproducible and easy to perform (Kremer et al., 1999);particularly spoligotypes can be digitized into binary or octal code formats (Dale et al., 2001) which greatly facilitate the management and interlaboratory comparison of spoligotyping data. ...

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EVOLUTION AND CLONAL TRAITS OF MYCOBACTERIUM TUBERCULOSIS COMPLEX IN GUINEA-BISSAU

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G Källenius, T Koivula… - Journal of clinical …, 1999 - Am Soc Microbiol... synthetic spacer oligonucleotides which are covalently bound to a membrane (Isogen Bioscience BV ... of copies of IS6110 elements, and frequently lack the spoligotyping spacers 33 ... M .tuberculosisisolates in terms of biochemical traits, RFLP patterns, and spoligotypes when they ...

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MOLECULAR MARKERS DEMONSTRATE THAT THE FIRST DESCRIBED MULTIDRUG-RESISTANT MYCOBACTERIUM BOVISOUTBREAK WAS DUE TO MYCOBACTERIUM TUBERCULOSIS

[HTML] from nih.gov

MC Gutierrez, JC Galan, J Blazquez… - Journal of clinical …, 1999 - Am Soc Microbiol... (v) Characterization of the DR region by spoligotyping.The DR cluster DNA of the strains was amplified by PCR. The labeled PCR product was used as a probe to hybridize with 43 synthetic spacer oligonucleotides attached to a carrier membrane (Isogen Bioscience BV), as ...Cited by 31 - Related articles - BL Direct - All 9 versions - Import into BibTeX

 

USEFULNESS OF SPOLIGOTYPING TO DISCRIMINATE IS6110LOW-COPY-NUMBER MYCOBACTERIUM TUBERCULOSIS COMPLEX STRAINS CULTURED IN DENMARK

[HTML] from nih.gov

J Bauer, ÅB Andersen, K Kremer… - Journal of clinical …, 1999 - Am Soc Microbiol... culture stock) with glass beads by vortexing (26). Membranes for spoligotyping were obtained from Isogen, Bioscience BV, Utrecht, The Netherlands. The membranes contained oligonucleotides derived from the spacer DNA ...

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DNA TYPING OF A NONVIABLE CULTURE OF MYCOBACTERIUM TUBERCULOSIS IN A HOMELESS SHELTER OUTBREAK

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JR Driscoll, MA McGarry… - Journal of clinical …, 1999 - Am Soc Microbiol... Spoligotyping was performed on all the 1997 isolates from individuals involved in the outbreak,and from a sample of nonviable cells from the 1992 culture of patient A, by using a commercially available spoligotyping kit (Isogen, Maarssen, The Netherlands). ...

COMPARATIVE EVALUATION OF LIGATION-MEDIATED PCR AND SPOLIGOTYPING AS SCREENING METHODS FOR GENOTYPING OFMYCOBACTERIUM TUBERCULOSIS STRAINS

[HTML] from nih.gov

S Bonora, MC Gutierrez, G Di Perri… - Journal of clinical …, 1999 - Am Soc Microbiol... (i) Spoligotyping.Spoligotyping was performed ... probe to hybridize with 43 synthetic spacer oligonucleotides attached to a carrier membrane (Isogen Bioscience BV ... The software-assisted evaluation of spoligotypes was done with GelCompar software, version 3.1b (Applied Maths ...

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EXTENSIVE CROSS-CONTAMINATION OF SPECIMENS WITHMYCOBACTERIUM TUBERCULOSIS IN A REFERENCE LABORATORY

[HTML] from nih.gov MC Ramos, H Soini, GC Roscanni… - Journal of clinical …, 1999 - Am Soc Microbiol... Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen,The Netherlands ... obtained between 8 May and 18 July 1995 were characterized by spoligotyping and IS6110 ... Seventeen different spoligotypes were identified among the 91M. ...

 

1998

 

TOWARDS A STANDARDIZED APPROACH TO DNA FINGERPRINTING OF MYCOBACTERIUM BOVIS

DV Cousins, RA Skuce, RR Kazwala… - … of Tuberculosis and …, 1998 - ingentaconnect.com... in word processing program Perform PGRS RFLP on common Spoligotypes - use Alu I digest ...Spoligotyping A standard set of oligonucleotides for amplification of spacer regions as ... A commercially available kit (Isogen, Bioscience BV, The Netherlands), containing spoligotyping ...

DRUG RESISTANCE RATES OF MYCOBACTERIUM TUBERCULOSIS STRAINS IN AUSTRIA BETWEEN 1995 AND 1998 AND MOLECULAR TYPING OF MULTIDRUG-RESISTANT ISOLATES

F Stauffer, A Makristathis, JP Klein… - Epidemiology and …, 2000 - Cambridge Univ Press... se- quences of M. tuberculosis H37Rv and M. bo is BCG P3, being immobilized on a membrane delivered by Isogen Bioscience (Maarssen ... 1. Spoligotyping of MDR MTB strains isolated be-tween 1995 and 1998 in Austria: Lanes 1 and 19 represent the spoligotypes of the ...

[PDF] MYCOBACTERIOSIS IN WILD BOAR: RESULTS OF 2000-2006 ACTIVITY IN NORTH-WESTERN ITALY [PDF] from vet-alfort.fr A Dondo, S Zoppi, F Rossi, L Chiavacci, A Barbaro… - aeema.vet-alfort.fr... 37 a PCR based reverse line blot method covalently (Isogen, Netherland). M. bovis strains isolatedwere further characterized by VNTR typing (ETR A, B, C, D, E) according to Frothingham[Frothingham et al., 1998]. The strains unidentified by Spoligotyping were processed by a ..

N 1995 AND 1998 AND MOLECULAR TYPING OF MULTIDRUG-RESISTANT ISOLATES

F Stauffer, A Makristathis, JP Klein… - Epidemiology and …, 2000 - Cambridge Univ Press... se- quences of M. tuberculosis H37Rv and M. bo is BCG P3, being immobilized on a membrane delivered by Isogen Bioscience (Maarssen ... 1. Spoligotyping of MDR MTB strains isolated between 1995 and 1998 in Austria: Lanes 1 and 19 represent the spoligotypes of the ...

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[PDF] MYCOBACTERIOSIS IN WILD BOAR: RESULTS OF 2000-2006 ACTIVITY IN NORTH-WESTERN ITALY

[PDF] from vet-alfort.fr A Dondo, S Zoppi, F Rossi, L Chiavacci, A Barbaro… - aeema.vet-alfort.fr... 37 a PCR based reverse line blot method covalently (Isogen, Netherland). M. bovis strains isolated were further characterized by VNTR typing (ETR A, B, C, D, E) according to Frothingham[Frothingham et al., 1998]. The strains unidentified by Spoligotyping were processed by a ...

[PDF] INFORME FINAL DE PROYECTO DE INVESTIGACIÓN

[PDF] from ins.gob.pe EN MULTIDROGORESISTENCIA… - bvs.ins.gob.pe... Salud e interpretadas en el software ALFWin TM Sequence Analyser 2.0 1998. Ensayode genotipificación mediante Spoligotyping Fue usado un kit comercialmente disponible (Isogen Bioscience BV, Maarssen, The Netherlands ...