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Sickle cell anaemia is an inherited blood disorder, with ~50% of the global cases arising in India. The condition is caused by a faulty "sickle gene" which is inherited in autosomal recessive mode- a single copy of this gene causes sickle cell trait (benign) and two copies cause sickle cell anaemia.(Haemoglobinopathies/Blood disorders)

Pro-thrombin and Factor V Leiden

Target variant

G-A transition at nucleotide 20210 in the 3' untranslated region of the prothrombin geneF2 3'UTR F, F2 3'UTR R


G-A transition at nucleotide 1691 in exon 10F5 EX10F, F5 EX10R

Sample type:

  1. Molecular analysis : 2 ml of blood in an EDTA vacutainer (purple cap) collected aseptically.
  2. Prenatal Diagnosis : Chorionic villus sample (30-40 mg) or Amniotic fluid (20-30 ml ) or Purified DNA ( 2-3micro micrograms ) or Fetal chord blood sample ( 1-2 ml).

Transportation :

The samples may be sent at room temperature within 48 hrs from the sample collection time.


7-10 Days

Note :

TRF + Form G + Form F + Clinical history*

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