- TB testing
TB Is Not To Be
At Mapmygenome, we understand that TB diagnostics can be a tricky business. There are many tests available, each with its own set of limitations. We understand the need for an efficient and effective diagnostic test that can give accurate results quickly, at a reasonable cost. We offer PCR and RT PCR based solutions, with proven sensitivity and specificity. Our products include
Spoligo TBTM is a CE-Certified, effective PCR-based method to simultaneously detect and categorize Mycobacterium tuberculosis complex. Spoligo TBTM utilizes the technology of DNA polymorphism, which is present at a particular chromosomal locus, the Direct Repeat (DR) region, which is unique to Mycobacterium tuberculosis complex bacteria.
SMART Tuberculosis is a Realtime PCR method for detection of the mycobacterium tuberculosis Complex. SMART Tuberculosis and its components are suitable for clinical and diagnostic labs. A complete, ready-to-use master mix is supplied with the kit. Users just have to add the extracted DNA sample to the master mix and run the test.
When run according to the instructions, members of the Tuberculosis Complex (M. tuberculosis, M. bovis, M. canetti, M. microti, M. pinnipedii, and M. africanum) are detected through a conserved region in the DNA gene coding for 16s RNA. It is specific for members of the Tuberculosis Complex and does not detect non-tuberculosis Mycobacterium.
Expertise in TB diagnostic kits
Our SpoligoTB TM kits are used by world-class research and diagnostic labs across the globe. This is a list of published studies that used SpoligoTB kits to identify and type Mycobacterium tuberculosis.
Veterinary TB genotyping
Barandiaran, Soledad & Vivot, Marcela & Falzoni, Elvira & Marfil, Maria & Pérez Tort, Gabriela & Rovatti, Paula & Fernández, Mónica & Iachini, R.H. & Satek, Fernanda & Duchene, Adriana & Zumarraga, Martin. (2017). Mycobacterioses in dogs and cats from Buenos Aires, Argentina. Journal of Veterinary Diagnostic Investigation. 29. 104063871771379. doi:10.1177/1040638717713795.
Drug resistant TB in HIV infection
Neesha Rockwood, Frederick Sirgel, Elizabeth Streicher, Robin Warren, Graeme Meintjes, Robert J Wilkinson; Low Frequency of Acquired Isoniazid and Rifampicin Resistance in Rifampicin-Susceptible Pulmonary Tuberculosis in a Setting of High HIV-1 Infection and Tuberculosis Coprevalence, The Journal of Infectious Diseases, Volume 216, Issue 6, 15 September 2017, Pages 632–640, https://doi.org/10.1093/infdis/jix337
Zheng Jie Marc Ho, Cynthia Bin Eng Chee, Rick Twee-Hee Ong, Li Hwei Sng, Woei Ling Justine Peh, Alex R. Cook, Li Yang Hsu, Yee Tang Wang, Han Fang Koh, Vernon Jian Ming Lee, Investigation of a cluster of multi-drug resistant tuberculosis in a high-rise apartment block in Singapore, International Journal of Infectious Diseases, Volume 67, 2018, Pages 46-51, ISSN 1201-9712, https://doi.org/10.1016/j.ijid.2017.12.010.
Molecular typing of Pulmonary TB
Bedewi Z, Worku A, Mekonnen Y, et al. Molecular typing of Mycobacterium tuberculosis complex isolated from pulmonary tuberculosis patients in central Ethiopia. BMC Infectious Diseases. 2017;17:184. doi:10.1186/s12879-017-2267-2.
Molina-Moya B, Lacoma A, García-Sierra N, et al. PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping. Scientific Reports. 2017;7:6777. doi:10.1038/s41598-017-06760-5.
MTB isolates from Central India
Desikan P, Chauhan DS, Sharma P, et al. Genetic diversity of Mycobacterium tuberculosis isolates from central India. The Indian Journal of Medical Research. 2016;143(4):481-486. doi:10.4103/0971-5916.184287.