- R&D Services
Real-Time Assays (qPCR)
Quantitative PCR (qPCR) or Real-Time PCR assays is a reaction that monitors the amplification of a targeted DNA molecule during the PCR in real-time, usually as a fluorescence intensity and not at its end, as in conventional PCR. These assays could be based on SYBR dye or TaqMan chemistry. Compared against end-point PCR approaches, qPCR provides superior sensitivity and precision.
Mapmygenome provides qPCR services using platforms:
Step-one Real-Time PCR machine (ThermoFisher Scientific).
7500 Real-Time PCR Machine (ThermoFisher Scientific).
Quantstudio (Open array system, ThermoFisher Scientific).
Gene expression analysis (validation of Microarray and NGS data, custom gene expression profiling depending on the client needs)
Splice variant analysis (a splice site mutation affects the amount of mRNA synthesis and qPCR analysis helps to understand these changes)
Diagnostic studies for infectious diseases such as TB
SNP Genotyping/allelic discrimination: A custom design of a set of SNPs (16-256 SNPs) can be analyzed in study subjects for client requirements/research studies
Microarray & NGS Services
Microarray Services: Gene expression profiling is provided using Agilent microarray platform
NGS: Whole Genome Sequencing and Whole Exome Sequencing services are provided - from sample preparation to data analysis